Oncology Probes

KREAvital Bone Marrow Karyotyping MediumAn increasing number of cytogenetic analyses are carriedout using bone marrow aspirates for studying chromosomalabnormalities in hematology.KREAvital Bone Marrow Karyotyping Medium is intended foruse in short-term cultivation of primary bone marrow cells forchromosome evaluation and has been optimized for providinga high mitotic index. It is based on RPMI-1640 basal mediumsupplemented with L-Glutamine, fetal bovine serum, andantibiotics. The medium does not contain any mitogens orconditioned medium.ColchicineColchicine is a secondary metabolite originally extracted fromplants of the genus Colchicum. In cell biology it is traditionallyused as a mitosis inhibitor to arrest cells in metaphase andallowing cell harvest and karyotyping to be performed.ColcemidColcemid, also known as demecolcine, is related to colchicine,but less toxic, and therefore increasingly popular as anequivalent mitosis inhibitor in cytogenetics.LymphocytesKREAvital Myeloid Cell MediumFresh cells or cells grown in short-term cultures often yield aninsufficient number of mitotic cells and repeated aspirationsare required. KREAvital Myeloid Cell Medium was developedto stimulate the proliferation of human hematopoietic cellsfrom bone marrow as well as peripheral blood. This medium isparticularly effective for karyotyping of acute non-lymphocyticleukemias and various stages of chronic myelogenousleukemia as well as other hematological disorders such asmyelodysplastic syndrome and polycythemia vera. KREAvitalMyeloid Cell Medium is based on MEM-Alpha basal mediumsupplemented with L-Glutamine, fetal bovine serum, antibioticsand conditioned medium.All KREAvital media include L-Glutamine and antibioticsTo secure rigorous quality assurance, each KREAvital batch is tested for cell growth in aleading clinical cytogenetics laboratory.Cell Culture Media95