12.07.2015 Views

Oncology Probes

Oncology Probes

Oncology Probes

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cell culture mediaKREAvital cytogenetic mediaOur product line KREAvital and related products have been carefully selected to optimally addresscytogenetic applications. They have been further optimized for best performance and undergo strict qualityprocedures ensuring consistent performance and superior results.KREAvital Prenatal Medium (Complete)The in vitro cultivation of amniotic fluid cells and chorionic villiis an essential part of every diagnostic cytogenetics laboratory,since the preparation of metaphase chromosome spreads isdependent upon obtaining cells in division. Amniocentesisand chorionic villi sampling are the major invasive diagnosticprocedures used for the detection of fetal chromosomalabnormalities. KREAvital Prenatal Medium is specificallyoptimized for the primary culture of human amniotic fluidcells and chorionic villi samples used in prenatal diagnostictesting. The medium is contains serum, glutamine andantibiotics, and greatly reduces karyotyping time comparedto conventional media.In addition to the complete ready-to-use medium, we alsoprovide KREAvital Prenatal as basal medium and supplementas separate components.Human AmniocytesCell Culture Media94KREAvital Prenatal Medium PLUS (Complete)Growing cells from amniotic fluids yield a mixture of epithelialcells, fibroblasts and amniocytes. KREAvital Prenatal MediumPLUS is an optimized formulation specifically enriching forfibroblasts and amniocytes, which are the cells best suitedfor genetic analysis. This circumstance ensures optimalchromosome morphology and metaphase structure formicroscopic observation, while reducing the relatively highbackground generated from having too much epithelialcells.KREAvital Prenatal Medium PLUS provides• Less epithelial cells• Clearer chromosome morphology for optimal bandinganalysis• Enhanced buffering capacity in closed systems• Prolonged stability when stored at 4°CKREAvital Lymphocyte Karyotyping MediumKREAvital Lymphocyte Karyotyping Medium is intended foruse in short-term cultivation of peripheral blood lymphocytesfor chromosome evaluation. It is based on RPMI-1640 basalmedium supplemented with L-Glutamine, fetal bovine serumand antibiotics. We provide this medium with and without theaddition of phytohaemagglutinin (PHA), respectively.

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