Oncology Probes

PreimpScreen PolB (13,16,18,21,22)PreimpScreen PolB is designed for determining chromosomecopy number in polar bodies.PreimpScreen Blas (13,18,21,X,Y)PreimpScreen Blas is designed for determination of chromosomecopy number in blastomeres.The first polar body is removed from the unfertilized oocyte,and the second polar body from the zygote, shortly afterfertilization. The main advantage of the use of polar bodiesin preimplantation genetic screening (PGS) is that theyare not necessary for successful fertilization or normalembryonic development, thus ensuring no deleterious effectfor the embryo. In some countries, where the legislationbans the selection of preimplantation embryos, polar bodyanalysis is the only possible method to perform PGS. Thebiopsy and analysis of the first and second polar bodies canbe completed before syngamy, which is the moment fromwhich the zygote is considered an embryo and becomesprotected by the law.Cleavage-stage biopsy is generally performed the morningof day three post-fertilization, when normally developingembryos reach the eight-cell stage. The biopsy is usuallyperformed on embryos with less than 50% of anucleatedfragments and at an 8-cell or later stage of development.The main advantage of cleavage-stage biopsy over polarbody (PB) analysis is that the genetic input of both parentscan be studied, and therefore currently is the prevalentmethod when doing in situ hybridizations in preimplantationgenetic screening.Pseudo color image using PreimpScreen PolB (KBI-40050) on ametaphase spread from lymphocytes showing two signals each ofchromosomes 13, 16, 18, 21, and 22, respectively.Ordering informationPseudo-color image on a healthy female blastomer usingPreimpScreen Blas (13,18,21,X,Y) FISH panel, KBI-40051.Image kindly provided by Prof. D. Griffin, University of Kent, United KingdomProduct Description Tests Cat#PreimpScreen PolB (13,16,18,21,22) Five-color FISH-mix consisting of DNA probes specific for chromosomes 13, 16, 18, 21, and 22 20 KBI-40050PreimpScreen Blas (13,18,21,X,Y) Five-color FISH-mix consisting of DNA probes specific for chromosomes 13, 18, 21, X, and Y 20 KBI-40051MultiStar 24 FISHFISH probe panel for visualizing all 24 chromosomes (including the four panels KBI-40061,KBI-40062, KBI-40063, and KBI-40064)10 KBI-40060MultiStar FISH Panel 1 FISH panel of centromeric probes for chromosomes 1, 3, 4, 6, 7, and 8 10 KBI-40061MultiStar FISH Panel 2 FISH panel of centromeric probes for chromosomes 9, 10, 11, 12, 17, and 20 10 KBI-40062MultiStar FISH Panel 3 FISH panel of centromeric probes for chromosomes 2, 15, 16, 18, X, and Y 10 KBI-40063MultiStar FISH Panel 4 FISH panel of unique sequence probes for chromosomes 5, 13, 14, 19, 21, and 22 10 KBI-40064Preimplantation Genetic Screening67