Oncology Probes

ON FUS (16p11) BreakThe FUS gene was originally shown to be rearranged in myxoidliposarcomas harboring a t(12;16)(q13;p11) translocation.FUS has been shown to be involved also in otherrecombinations: with ERG in acute myeloid leukemia carryinga t(16;21), with ATF1 in band 12q13 in angiomatoid fibroushistiocytoma, and with CREB3L2 in fibromyxoid sarcoma.A break or split probe for FUS is best used to analyzetranslocation of the FUS (16p11) gene on formalin fixedparaffin embedded tissue for routine clinical diagnosis.ON FKHR (13q14) BreakThe t(2;13) is associated with alveolar rhabdomyosarcomas.This translocation results in the formation of a chimerictranscript consisting of the 5’ portion of PAX3, includingan intact DNA-binding domain fused to the FKHR gene onchromosome 13. The t(1;13)(p36;q14) also seen in alveolarrhabdomyosarcomas results in the fusion of anothermember of the PAX family, PAX7 to the FKHR gene onchromosome 13.A break or split probe for FKHR is best used to analyzetranslocation of the FKHR (13q14) gene on formalin fixedparaffin embedded tissue for routine clinical diagnosis.Cat.# KBI-10715 FUS (16p11)Cat.# KBI-10716 FKHR (13q14) BreakD16S2574ERH 4517816p11780 KB840 KBFUS13q14FKHR(FOXO1A)16D16S2933E550 KB13D13S647690 KBFUS (16p11) Break probe hybridized to liposarcomamaterial (2RG).Literature:Shing et al, 2003, Cancer Res, 63: 4568-4576.Storlazzi et al, 2003, Hum. Mol. Genet., 12: 2349-2358.Ordering information Color Tests Cat#ON FUS (16p11), Break red/green 10 KBI-10715FKHR (13q14) Break probe hybridized to a normalmetaphase (2RG).Literature:Barr et al, 1996, Hum. Mol. Genet., 5; 15-21.Coignet et al, 1999, Genes Chrom. Cancer, 25; 222-229.Ordering information Color Tests Cat#ON FKHR (13q14) Break red/green 10 KBI-10716Oncology Probes - Solid Tumors57