Oncology Probes

SarcomaSarcoma is a general class of less common cancersin which the cancer cells arise from or resemblenormal cells in the body known as “connectivetissues” (fat, muscle, blood vessels, deep skintissues, nerves, bones, and cartilage). The benignand malignant forms have related karyotypicchanges which provide an important resource foridentifying the additional genetic changes thatoccur in the malignant compared with the benignform. In fact, the molecular biology of soft-tissuesarcomas has provided the perfect example ofhow cytogenetic and molecular approaches cancontribute toward a clearer understanding of thedevelopment of soft-tissue sarcomas.ON EWSR1 (22q12) BreakThe translocation t(11;22)(q24;q12) has been independentlydescribed by several groups in the malignant cells of patientswith Ewing sarcoma and can be detected in more than 90%of these tumors. The translocation involves the fusion ofthe human FLI1 gene on chromosome 11, with the codingsequence of the EWS gene on chromosome 22. In about 5%of the cases, however, the EWS gene is involved in varianttranslocations. These translocations, t(21;22)(q12;q12) andt(7;22)(p22;q12) result in the fusion of EWS with ERG andETV1, respectively.The Poseidon break or split probe for EWS is optimizedto identify translocation involving the EWS gene onformalin fixed paraffin embedded tissue for routine clinicaldiagnosis.Cat.# KBI-10708 EWS (22q12) BreakRH 120772760 KB22q12EWS221200 KBD22S1023EWS (22q12) Break probe hybridized to patient materialshowing a translocation involving the EWRS1 gene region at22q12 (1RG1R1G).Literature:Zucman-Rossi, et al, 1998, PNAS, 95; 11786-11791.Bernstein et al, 2006, Oncologist, 11; 503-519.Ordering information Color Tests Cat#ON EWSR1 (22q12) Break red/green 10 KBI-10708Oncology Probes - Solid Tumors55