Oncology Probes

Breast CancerON ERBB2, Her2/Neu (17q12) / SE 17The Her2/Neu gene encodes a receptor tyrosine kinase involvedin growth factor signaling. Overexpression of this gene is seen inabout 20% of invasive breast cancers and is associated with poorsurvival. Her2 gene amplification is a permanent genetic changethat results in this continuous overexpression of Her2. A drugcalled trastuzumab (commonly known as Herceptin) has beendeveloped to be effective against Her2-positive breast cancer.Her2/Neu amplification is also observed in a variety of othertumors, such as prostate, lung, colon and ovary carcinoma.The Her2/Neu (17q12) specific DNA probe is optimized to detectcopy numbers of the Her2/Neu (ERBB2) gene region at region17q12. The chromosome 17 satellite enumeration probe (SE 17)at D17Z1 is included to facilitate chromosome identification.ON TOP2A (17q21) / SE 17The Topoisomerase2A enzyme, which is vital for the cellbecause of its role in cell replication and repair, catalyzesthe relaxation of supercoiled DNA molecules to create areversible double-strand DNA break. This enzyme is alsothe target of a number of cytotoxic agents, namely TOP2Ainhibitors (anthracyclines, etoposide, teniposide).The dual-color probe is optimized to detect amplifications(copy numbers) or deletions of the TOP2A gene region atthe 17q21. The chromosome 17 satellite enumeration probe(SE 17) at D17Z1 is included to facilitate chromosomeidentification.Cat.# KBI-10701 ERBB2, Her2/Neu (17q12) / SE 17Cat.# KBI-10724 TOP2A (17q21) / SE 17D17Z1RH 11280517q12D17Z1D17S123817q21ERBB2460 KBTOP2A360 KB17D17S70017D17S2011ERBB2, Her2/Neu (17q12) / SE 17 probe hybridized to breast tumortissue showing amplification of Her2/Neu (ERBB2)/ SE 17.Literature:Pauletti et al, 1996, Oncogene, 13: 63-72.Xing, et al, 1996, Breast Cancer Res Treat, 39: 203-212.Ordering information Color Tests Cat#ON ERBB2, Her2/Neu (17q12) / SE 17 red/green 10 KBI-10701ON ERBB2, Her2/Neu (17q12) / SE 17 red/green 50 KBI-14701TOP2A (17q21) / SE 17 probe hybridized to breast tissue (2R2G).Literature:Järvinen et al, 1999, Genes, Chromosomes and Cancer 26; 142-150Järvinen et al, 2000, Am. J. Pathology 156; 639-647Ordering information Color Tests Cat#ON TOP2A (17q21) / SE 17 red/green 10 KBI-10724Oncology Probes - Solid Tumors47