Oncology Probes

ON RARA (17q21) BreakThis break apart probe can detect the numerous types ofrecurrent rearrangement of the RARα (Retinoid acid receptor,alpha) gene with various gene partners (e.g., PML, NPM,MLL, FIP1L1, NuMA1, PLZF, amongst the others), leadingto the formation of different reciprocal fusion proteins. Theimportance of retinoid metabolism in acute promyelocyticleukemia (APL) is highlighted by the numerous recent studies,but the different leukemogenic functions of the RARα fusionproteins in the neoplastic myeloid development still has tobe defined, as well as the distinct clinical outcome of thepatients with the variant forms of APL.ON CBFB t(16;16); inv(16) BreakInv(16)(p13;q22) and t(16;16)(p13;q22) are recurringchromosomal rearrangements in AML. In both the inversionand translocation, the critical genetic event is the fusion ofthe CBFB gene at 16q22 to the smooth muscle myosin heavychain (MYH11) at 16p13. A deletion of between 150 and350 kb centromeric to the p-arm inversion breakpoint clusterregion can be observed in some patients containing the 5’portion of the myosin heavy chain (MYH11) gene.The CBFB t(16;16) inv(16) break probe is optimized to detectthe inversion of chromosome 16 involving the CBFB generegion at 16q22 in a dual-color, split assay.Cat.# KBI-10305 RARA (17q21) BreakCat.# KBI-10304 CBFB t(16;16); inv(16) BreakD17S214717q21RARA530 KBRH113713CBFB275 KB17D17S1196610 KB1616q22RH32600335 KBRARA (17q21) Break probe hybridized to patient materialshowing a translocation at 17q21 (1RG1R1G).Ordering information Color Tests Cat#ON RARA (17q21) Break red/green 10 KBI-10305CBFB t(16;16); inv(16) Break probe hybridized to a normalmetaphase (2RG).Literature:Dauwerse et al, 1993, Hum.Mol.Genet., 2: 1527-1534.Marlton et al, 1995, Blood, 85: 772-779.Ordering information Color Tests Cat#ON CBFB t(16;16); inv(16) Break red/green 10 KBI-10304Oncology Probes - Hematology Probes31