Oncology Probes

ON EVI t(3;3); inv(3) (3q26) BreakThe inv(3)(q21;q26) is a recurrent cytogenetic aberration ofmyeloid malignancy associated with fusion of EVI1 and RPN1and a poor disease prognosis. Genomic breakpoints in 3q26 areusually located proximal to the EVI1 locus, spanning a regionof several hundred kilobases. Other recurrent and sporadicrearrangements of 3q26 also cause transcriptional activationof EVI1 including the translocations t(3;3)(q21;q26) and t(3;21)(q26;q22). Breakpoints in the latter rearrangements spana wider genomic region of over 1 megabase encompassingsequences distal to EVI1 and neighboring gene MDS1.ON EVI t(3;3); inv(3), EVI (3q26) Break, TCThe EVI t(3;3) inv(3) Break, triple-color probe is optimized todetect the inversion of chromosome 3 involving the EVI generegion at 3q26 in a dual-color, split assay on metaphase/interphase spreads, blood smears and bone marrow cells. Byusing a third color breakpoint variations can now be easilydiscovered.The EVI t(3;3) inv(3) Break, dual-color probe is optimized todetect the inversion of chromosome 3 involving the EVI1gene region at 3q26 in a dual-color, split assay on metaphase/interphase spreads, blood smears and bone marrow cells.Cat.# KBI-10204 EVI t(3;3); inv(3) (3q26) BreakCat.# KBI-10205 EVI t(3;3); inv(3) (3q26), Triple-ColorRH103072420 KBD3S1243460 KBEVI1hTERC370 KBEVI1RH10606hTERC3q26.2370 KB3q26.2550 KBRH1060633RH123089Oncology Probes - Hematology ProbesEVI t(3;3);inv(3) (3q26) Break probe hybridized to patientmaterial showing a rearrangement involving the EVI gene regionat 3q26 (1RG1R1G).Image kindly provided by Dr. Reed, LondonLiterature:Levy et al, 1994, Blood, 83: 1348-1354.Wieser et al, 2003, Haematologica, 88: 25-30.Melo et al, 2007, Leukemia, 22, 434-437.Ordering information Color Tests Cat#ON EVI t(3;3); inv(3) (3q26) Break red/green 10 KBI-10204EVI t(3;3); inv(3) (3q26) TC probe hybridized to patientmaterial showing a rearrangement involving the EVI gene regionat 3q26 (1RGB1B1RG).Image kindly provided by Prof Jauch, HeidelbergLiterature:Levy et al, 1994, Blood, 83: 1348-1354.Wieser et al, 2003, Haematologica, 88: 25-30.Melo et al, 2007, Leukemia, 22, 434-437.Ordering information Color Tests Cat#ON EVI t(3;3); inv(3) (3q26) Break, TC red/green/blue 10 KBI-1020528Note: In t(3;3) the breakpoint cluster can span 1.3 Mb region. The described probe set willtherefore provide false negative results in cases with very distal breakpoints.