Oncology Probes

ON hTERT (5p15) / 5q31The hTERT / 5q31 dual-color probe can be used to detectdeletions involving band 5q31 in MDS and AML.The 5q- specific DNA probe is optimized to detect copynumbers at the CDC25C/EGR1 gene region at 5q31. The hTERTgene region at 5p15 is included to facilitate chromosomeidentification.ON MDS 5q- (5q31; 5q33)The presence of del(5q), either as the sole karyotypicabnormality or as part of a more complex karyotype, hasdistinct clinical implications for myelodysplastic syndromes(MDS) and acute myeloid leukemia. Interstitial 5q deletions arethe most frequent chromosomal abnormalities in MDS and arepresent in 10% to 15% of MDS patients. Two different criticalregions are described, one at 5q31-q33 containing the CSF1Rand RPS14 gene regions ,characteristic for the ‘5q-‘ syndrome,and a more proximal located region at 5q13-q31 containingthe CDC25C and EGR1 gene regions.The 5q- specific DNA probe is optimized to detect copy numbersat the CDC25C/EGR1 gene region at 5q31 and the CSF1R/RPS14gene region at 5q33 simultaneously in a dual-color assay.Cat.# KBI-10208 hTERT (5p15) / 5q31Cat.# KBI-10209 MDS 5q- (5q31; 5q33)5p15.3RH48032D5S1981hTERTD5S2269190 KBCDC25CEGR1D5S597645 KB5q31.25RH480325q31.2CDC25CEGR1645 KB5D5S2618CSF1R5q33.1 RPS14D5S2014650 KBD5S597hTERT (5p15) / 5q31 probe hybridized to a normal interphase/metaphase (2R2G).Literature:Zhao et al, 1997, PNAS, 94; 6948-6053Horrigan et al, 2000, Blood, 95; 2372-2377Ordering information Color Tests Cat#ON hTERT (5p15) / 5q31 red/green 10 KBI-10208MDS 5q- (5q31; 5q33) probe hybridized to patient materialshowing a 5q33 deletion (1R2G).Image kindly provided by Dr Mohr, DresdenLiterature:Boultwood J e.a., Blood 2002; 99: 4638-4641Zhao N e.a., PNAS 1997; 94: 6948-6953Wang e.a., Haematologica 2008; 93: 994-1000Ebert BL e.a., Nature 2008: 451: 335-339Mohamedali A and Mufti GJ, Brit J Haematol 2008; 144: 157-168Ordering information Color Tests Cat#ON MDS 5q- (5q31; 5q33) red/green 10 KBI-10209Oncology Probes - Hematology Probes25