Oncology Probes

Technical AppendixThe Poseidon Probe MapsAll locus specific probes are provided with a map showingthe genome region and/or genes the probe will cover. The STSmarkers indicating the most proximal and distal end coveredby the probe are added as well. Those markers can be used todescribe in situ hybridization results in accordance with theInternational System for Human Cytogenetic Nomenclature(ISCN, 2009). For more information see e.g. Genome Browserat genome.ucsc.edu or Ensemble at www.ensembl.orgRB1Most proximal marker covered by probeD13S147713q14 RFP2DLEU1D13S272650 KB220 KBTarget Gene (or Genes) covered by ProbeProbe size and color indication13D13S25450 KBProbe maps not to scaleMost distal marker covered by probeDistance to another region or gene of interestKreatech’s fluorophores and filter recommendationsStandard fluorophores used forPoseidon FISH probesFluorophore Color Excitation/EmissionPlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright550 Red 550/580Fluorophores used in PlatinumBright andFISHBright Labeling KitsFluorophore Color Excitation/EmissionPlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright505 Green 500/528PlatinumBright550 Red 550/580Technical Appendix112Fluorophores used in PGS kits(KBI-40050; 40051; 40060-40064)Fluorophore Color Excitation/EmissionPlatinumBright405 Dark Blue 410/455PlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright547 Light Red 547/565PlatinumBright590 Dark Red 587/612PlatinumBright647 Far Red 647/665PlatinumBright547 Light Red 547/565PlatinumBright570 Dark Red 570/591PlatinumBright647 Far Red 647/665When ordering a filter from Chroma or other suppliers pleasemake sure to either have a cube or slider available for yourspecific brand and type of microscope.Filters from other suppliers covering the same excitation andemission wavelengths as described above will also workperfectly with Poseidon probes. For further questions pleasecontact customerservice@kreatech.com