Oncology Probes

Antibody LABELINGPlatinumLink Antibody Labeling KitsThe PlatinumLink Kits provide high coverage of theproteome through specific labeling of methionine, cysteineand histidine residues. Unlike lysine, which is targeted byN-hydroxysuccinimidyl (NHS) esters, the amino acids labeledwith ULS are less likely to be involved in protein - proteininteractions. Therefore, labeling of proteins and antibodieswith ULS will reduce negative effects on interaction domainsand epitope recognition.The PlatinumLink Antibody Labeling Kits have been optimizedto label 100 μg of purified antibody (or recombinant protein)per reaction with fluorescent dyes or haptens.Unique features of the PlatinumLink AntibodyLabeling Kits• Easy and robust labeling of antibodies and recombinantproteins• ULS-Trap removes unreacted label, no size exclusion• Labeling of methionines, cysteines and histidines inantibodies and recombinant proteins• Labeling reaction is independent of pH• Compatible with commonly used detergents includingTris-HClSchematic overview of the PlatinumLink labeling procedure.Recovery comparison of different proteins.Step 1: Protein labelingStep 2: Removal of unreacted ULS-label100100 µg proteinULS-label labeling buffer908070o/n 37°Clabeled sampleapply to ULS-Trap columncentrifuge 1 min60504030purified labeledprotein sample% recovery20100catalasebovinealbuminconalbumin anti-humanIgGanti-humanIgGanti-mouseIgGAntibody Labeling110Ordering informationFig.18 Protein recovery using ULS-Trap columns 100 µg of protein was labeled and purified using PlatinumLink protein labeling kits. Protein concentration was determined usingBiorad’s Rc Dc protein assay. Recovery is presented as percentage protein recovered ascompared to unpurified sample.Description Reactions Cat#PlatinumLink Protein Labeling Kit (BIO) 4 single labelings PLK-007PlatinumLink Protein Labeling Kit (FLU) 4 single labelings PLK-009PlatinumLink Protein Labeling Kit (RHO) 4 single labelings PLK-010