Oncology Probes

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RNA LABELINGAMPLIFICATION AND aRNA LABELING KITSaRNA Labeling KitsThe Universal Linkage System (ULS) allows direct labeling ofunmodified, amplified RNA (aRNA). Specific aRNA labeling kitsare both available for the use with commercial platforms fromAgilent, Affymetrix ® (Genechips ® ) and CombiMatrix, and forthe use with self-spotted DNA arrays.RNA ampULSe KitsThe RNA ampULSe kit combines two successful technologies.The aRNA is generated with Ambion’s MessageAmp II aRNAamplification reagents followed by non-enzymatic labelingwith KREATECH’s proprietary Universal Linkage System (ULS)technology. Specific ampULSe kits are both available for theuse with commercial platforms from Agilent, Affymetrix ®(Genechips ® ) and CombiMatrix, and for the use with selfspottedDNA arraysUnique features of aRNA Labeling Kits• Higher yields of unmodified aRNA compared to modifiedaRNA• Storage of unmodified aRNA for subsequent use possible• No waste of labeled material; label only the amount aRNAneeded• Reduced labeling cost per hybridization when usingmultiple arrays per slideHigher yields of unmodified aRNA as compared to modified aRNALower cost if multiple arrays per slide are used160unmodified aRNAmodified aRNA100ULS labelingEnzymatic labelingaRNA yield (µg)140120100806040200HeLa U2OS JurkatHuman samples (starting with 5 µg total RNA)Relative costs per sample(compared to 1 array/slide)90807060504030201001 2 4 8 12Number of arrays on 1 slideRNA LabelingUnmodified antisense RNA (aRNA) vs. modified aRNACosts for ULS labeling of unmodified aRNA vs. costs for enzymatic labelinggenerating modified aRNA106
microRNA Labeling KitThe new ULS microRNA Labeling Kit provides a very simple andfast method for miRNA detection on microarray platforms. TheUniversal Linkage System (ULS) labeling technology is used todirectly label the naturally occurring miRNAs which are present inthe total RNA mixture without the need to enrich for small RNA.ULS can be used to label total RNA from all kind of organisms.Unique features of the ULS microRNA Labeling Kit• 15 minutes labeling step• Easy two-step procedure to label total RNA• Compatible with most existing miRNA platforms• Not size discriminativeTime of ULS labeling vs. competitive labeling technologies.ULSlabelingclean up1412Competitor ACompetitor B0 h0,5 h1 h1,5 h2 h2,5 h3 h3,5 h4 h6,5hMedian Cy3 (logBGC)1086correlation coefficient= 0.996 8 10 12 14Median Cy5 (logBGC)Ordering informationDescription Contents Cat#RNA LabelingULS microRNA Labeling Kit (with Cy3 and Cy5) for labeling 2 x 25 µg RNA EA-036ULS microRNA Labeling Kit (with Cy3) for labeling 50 µg RNA EA-037ULS microRNA Labeling Kit (with Cy5) for labeling 50 µg RNA EA-038ULS aRNA Labeling Kit (with Cy3 and Cy5) for labeling 2 x 50 µg aRNA EA-006ULS aRNA Labeling Kit (with Biotin for Affymetrix® Genechips®) for labeling 500 µg aRNA EA-010ULS aRNA Labeling Kit (with Biotin) for labeling 250 µg aRNA EA-018ULS Fluorescent Labeling Kit for Agilent arrays (with Cy3 and Cy5) for labeling 2 x 50 µg aRNA EA-021ULS Fluorescent Labeling Kit for Agilent arrays (with Cy5) for labeling 50 µg aRNA EA-022ULS Fluorescent Labeling Kit for Agilent arrays (with Cy3) for labeling 50 µg aRNA EA-023ULS Labeling Kit for CombiMatrix arrays (with Cy5) for labeling 125 µg aRNA EA-025ULS Labeling Kit for CombiMatrix arrays (with Biotin) for labeling 125 µg aRNA EA-027RNA Amplification and Labeling (20 amplifications each)RNA ampULSe Amplification and Labeling Kit (with Cy3 and Cy5) for labeling 2 x 20 µg aRNA GEA-001RNA ampULSe Amplification and Labeling Kit (with Biotin for Affymetrix® Genechips®) for labeling 40 µg aRNA GEA-004RNA ampULSe Amplification and Labeling Kit (with Biotin) for labeling 200 µg aRNA GEA-006RNA ampULSe Amplification and Labeling Kit (with Cy3 and Cy5 for Agilent arrays) for labeling 2 x 20 µg aRNA GEA-014RNA ampULSe Amplification and Labeling Kit (with Cy5 for Agilent arrays) for labeling 40 µg aRNA GEA-016RNA ampULSe Amplification and Labeling Kit (with Cy3 for Agilent arrays) for labeling 40 µg aRNA GEA-018RNA ampULSe Amplification and Labeling Kit (with Cy5 for CombiMatrix arrays) for labeling 100 µg aRNA GEA-022RNA ampULSe Amplification and Labeling Kit (with Biotin for CombiMatrix arrays) for labeling 100 µg aRNA GEA-026All RNA ampULSe Kits include Ambion’s MessageAmp II aRNA amplification reagents and the ULS-based labeling moduleRNA Labeling107
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KREATECH DiagnosticsProduct Guide
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Product Selection GuideI want to us
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HUGO Gene SymbolsThe table below gi
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Oncology probes - Hematology Probes
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Chronic Myeloproliferative Disorder
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ON BCR/ABL t(9;22), DC, S-Fusion, E
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Other Myeloproliferative Diseases:O
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Chronic Lymphocytic Leukemia (CLL)C
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ON hTERC (3q26) / 3q11Amplification
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ON hTERT (5p15) / 5q31The hTERT / 5
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ON MDS 7q- (7q22; 7q35) / SE 7 TCTh
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Acute Myeloid Leukemia (AML)At leas
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ON RARA (17q21) BreakThis break apa
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ON ETV6 (TEL) (12p13) BreakETV6 (TE
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ON MM 1q21 / 8p21Amplifications of
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ON MM 1q21 / SRD (1p36)Frequent los
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ON IGH (14q32) BreakChromosomal rea
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ON BCL6 (3q27) BreakChromosomal tra
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Oncology probes - Solid TumorsIn so
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ON TOP2A (17q21) / Her2 (17q12) / S
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Bladder CancerON p16 (9p21) / 9q21
Page 52 and 53:
NeuroblastomaAccording to the Inter
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ON p53 (17p13) / MPO (17q22) “ISO
Page 56 and 57: ON SYT (18q11) BreakThe characteris
Page 58 and 59: ON MDM2 (12q15) / SE 12Fibrosarcoma
Page 60 and 61: ON PTEN (10q23) / SE 10The gene ‘
Page 62 and 63: ON CCND1 (11q13) / SE 11CCND1 (also
Page 64 and 65: ON BCL6 (3q27) Break (tissue)Chromo
Page 66 and 67: Preimplantation Genetic ScreeningFI
Page 68 and 69: Prenatal probesPrenatal cytogenetic
Page 70 and 71: Microdeletion probesMicrodeletion s
Page 72 and 73: MD DiGeorge “TUPLE” (22q11) / 2
Page 74 and 75: MD NSD1 (5q35) / hTERT (5p15)NSD1 m
Page 76 and 77: MD Angelman UBE3A (15q11) / PML (15
Page 78 and 79: MD Wolf-Hirschhorn WHSC1 (4p16)Wolf
Page 80 and 81: MD STS (Xp22) / KAL (Xp22) / SE X T
Page 82 and 83: Sub-Telomere probesTelomeres are sp
Page 84 and 85: Satellite Enumeration probesThe pri
Page 86 and 87: Whole Chromosome probesThe Poseidon
Page 88 and 89: Arm Specific ProbesArm Specific Pro
Page 90 and 91: Mouse FISH ProbesNew applications f
Page 92 and 93: Chromogenic In Situ HybridizationCh
Page 94 and 95: cell culture mediaKREAvital cytogen
Page 96 and 97: PhytohaemagglutininPhytohaemaggluti
Page 98 and 99: Kits and ReagentsThe ready-to-use p
Page 100 and 101: FISHBright labeling kitsThe FISHBr
Page 102 and 103: Product Selection GuideULSArray CGH
Page 104 and 105: DNA LABELINGULS arrayCGH Labeling K
Page 108 and 109: PROTEIN LABELINGPROTEIN LABELING AN
Page 110 and 111: Antibody LABELINGPlatinumLink Antib
Page 112 and 113: Technical AppendixThe Poseidon Prob
Page 114 and 115: TERMS AND CONDITIONSTerms and Condi
Page 116 and 117: Product IndexOncology probes - Hema
Page 118 and 119: Satellite Enumeration probesSE = Sa
Page 120 and 121: Arm-Specific Probesready-to-useDesc
Page 122 and 123: Mouse FISHDescription Color Tests C
Page 124 and 125: DNA LabelingDescription Reactions C
Page 126 and 127: How To OrderHow to place an orderOr
Page 128: © 2010 KREATECH DiagnosticsPublish
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Oncology Probes

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