Oncology Probes

Array-Grade KREAcot DNAArray-Grade KREAcot DNA is extracted from human placentalDNA and subsequently fragmented, denatured, and re-annealedunder conditions that enrich for repetitive DNA sequences(1,2). Array-Grade KREAcot DNA can be used to suppresscross-hybridization to human repetitive DNA sequences. Array-Grade KREAcot DNA has specifically been optimized to be usedin array CGH applications. Amounts of Array-Grade KREAcotDNA needed in a microarray experiment should be determinedempirically, but will be in the order of 12.5-50 times excess tothe amount of labeled genomic DNA. Please visit the Kreatechwebsite (www.kreatech.com) for a detailed protocol for theanalysis of genomic DNA using array CGH.Megapool Reference DNAThis product is a homogeneous DNA pool from male orfemale human genomic DNA which has been isolated from100 different anonymous healthy individuals. The genomicDNA is of high quality and the DNA from each of these 100individuals contributes evenly to the DNA pool. This product isspecifically developed as a reference for genomic microarraybasedcomparative genomic hybridization experiments (arrayCGH).Unique features of Array-Grade KREAcot• Specifically optimized for use in array CGH applications• Quality controlled with array CGH analysisUnique features of the MegaPool Reference DNA• The product will guarantee perfectly comparable profiles formale and female individuals as well as complete absence ofpure homozygous deletions.• Sex-matched array CGH experiments will be possible andenable high-quality genotyping data for both chromosomesX and Y as well as unbiased CNV profiles between male andfemale test samples.Comparison of performance of Array-Grade KREAcot DNA andhuman C0t-1 DNA from competitor X.Ordering informationDescription Contents Cat#Array-Grade KREAcot2 log ratio for X chromosome = -0.65Mean absolute deviation forchrom 1-22 = 0.05Human C 0 t-1 DNA competitor X2 log ratio for X chromosome = -0.66Mean absolute deviation forchrom 1-22 = 0.11Shown are the chromosome plots obtained from an array CGH hybridization comparinghealthy male Cy5-ULS labeled genomic DNA and healthy female Cy3-ULS labeledgenomic DNA on BAC microarrays. Although the spread observed for the X-chromosomeis comparable for both blockers, the use of Array-Grade KREAcot minimizes the variationobserved for chromosomes 1-22.ULS cDNA synthesis and Labeling 10 dual labeling reactionsDSK-001Kit (with Cy3 and Cy5)(2 x 50 µg cDNA)ULS cDNA synthesis and Labeling 20 single labelingKit (with Cy5)reactions (100 µg cDNA)DSK-002ULS cDNA synthesis and Labeling 20 single labelingKit (with Cy3)reactions (100 µg cDNA)DSK-003ULS cDNA synthesis and Labeling 20 single labelingKit (with Biotin)reactions (100 µg cDNA)DSK-004ULS arrayCGH Labeling Kit for labeling 2 x 20 µg(with Cy3 and Cy5)DNAEA-005ULS arrayCGH Labeling Kit(with Cy3)for labeling 40 µg DNA EA-005AULS arrayCGH Labeling Kit(with Cy5)for labeling 40 µg DNA EA-005BArray-Grade KREAcot DNA 500 μg EA-020Array-Grade KREAcot DNA 10 mg EA-035Megapool Reference DNA (male) 200 µg EA-100MMegapool Reference DNA (female) 200 µg EA-100FDNA Labeling105