Oncology Probes

DNA LABELINGULS arrayCGH Labeling KitThe ULS arrayCGH Labeling Kit offers a novel procedurethat allows direct (non-enzymatic) labeling of both intactgenomic DNA as well as fragmented genomic DNA isolatedfrom formalin-fixed paraffin-embedded (FFPE) samples.The ULS arrayCGH Labeling Kit yields highly reproduciblefluorescent labeled DNA within minutes. The ULS technologyhas been validated on a wide range of array CGH platforms.All ULS kits for array CGH analysis include the KREApurepurification technology and KREAblock blocking reagents toensure efficient purification of labeled DNA samples and lowbackground levels during hybridization.ULS cDNA Synthesis and Labeling KitThe ULS cDNA Synthesis and Labeling Kit provides the optionto generate unmodified cDNA followed by direct labeling withthe ULS technology. These kits are superior to other enzymaticprotocols by allowing the use of natural nucleotides, thereforeresulting in better efficiency of the reverse transcriptase andreducing the chance of bias. The ULS cDNA Synthesis andLabeling Kits include all reagents for reverse transcription andsubsequent cDNA labeling.Unique features of ULS arrayCGH Labeling Kit• 30 minutes labeling procedure• Labeling independent of fragment size - ideal for FFPEsamples• ULS labeling is not affected by cross links present ingenomic DNA from FFPE samples• No enzymatic bias• KREApure column purification ideally suited forfragmented DNAUnique Features of the ULS cDNA Synthesisand Labeling Kit• Generation of cDNA with unmodified nucleotides to allowmost efficient transcription• Unmodified cDNA can be used for other assays• Labeling efficiency independent of fragment lengthULS Labeling vs Random Prime LabelingTime of ULS labeling vs. enzymatic labeling procedureA B triplo CcorrelationULScoefficientLabeling = 0.96ULSCompetitor Alabelingclean upD E triplo FDNA LabelingRandomcorrelationPrimecoefficientLabeling = 0.88Ratio plots of 3 independent array CGH hybridizations using genomic DNA from healthyliver which is the reference vs. genomic DNA isolated from a liver tumor FFPE sample.(A-C) ULS labeled samples. (D-F) Random Prime labeled samples.Competitor B0 h0,5 h1 h1,5 h2 h2,5 h3 h3,5 h4 h16 h104