Oncology Probes

FISHBright labeling kitsThe FISHBright labeling kits are based on KREATECH’s Universal Linkage System (ULS). The ULS moleculeis a proprietary platinum compound linked to a hapten or a fluorophore. The FISHBright kits offer ULSmolecules coupled to a variety of fluorophores or haptens providing a non-enzymatic, easy-to-use alternativeto conventional techniques such as nick translation, random priming, end labeling, etc.The FISHBright labeling kits are specially developed for FISH applications: labeled DNA probes can be usedon all type of samples including metaphase spreads, direct interphase cell preparation (e.g. blood smears,bone marrow smears), urine samples, paraffin-embedded or frozen tissue sections.KREATECH’s Poseidon probes are labeled with the same technology and all Poseidon pretreatment kits can beused in combination with DNA probes which are labeled with the FISHBright labeling kits.Besides the ULS technology the kits also contain Cell specific Hybridization Buffer (CHB), and TissueHybridization Buffer (THB). In addition, the KREAboost solution, which is developed to generate a high signalto-noiseratio especially on paraffin-embedded tissue sections, is included in these kits.The FISHBright labeling kits will offer you alladvantages of the ULS technology:• Simple to use• Labeling procedure within 30 minutes• Use of KREApure columns: a unique purification columnwhich guarantees a very high removal of non reacted ULS(> 99%) and high sample recoveries (> 95%).FISHBright labeling kitsContent:• PlatinumBright component (fluorochromes or haptens)• 10 x labeling solution• KREApure purification columns• Hybridization buffer for cytological preps (CHB)• Hybridization buffer for paraffin preps (THB+KREAboost)FISHBright Labeling KitsOrdering informationThe FISHBright kit contains sufficient solution to label up to10 μg of BAC DNA, which generally allows to perform 100 ormore FISH assays using 10 μl labeled probe in hybridizationbuffer (amount of FISH assays is dependent on the finalconcentration of labeled DNA. Usually 50 – 100 ng/μl of labeledBAC DNA is necessary per assay).Description Contents Cat#FISHBright 415 Blue for labeling 10 µg DNA FLK-001FISHBright 495 Green for labeling 10 µg DNA FLK-002FISHBright 505 Green for labeling 10 µg DNA FLK-003FISHBright 550 Red for labeling 10 µg DNA FLK-004FISHBright 547 Light Red for labeling 10 µg DNA FLK-005FISHBright 647 Far Red for labeling 10 µg DNA FLK-006FISHBright Biotin for labeling 10 µg DNA FLK-007100FISH Grade CoT 500 µg KB-COT