Texas, USA 2010 - International Herbage Seed Group
Texas, USA 2010 - International Herbage Seed Group Texas, USA 2010 - International Herbage Seed Group
APOSTART-derived SCAR markers discriminate between apomictic andsexual Poa pratensis L. genotypesE. Albertini, R. Torricelli and M. FalcinelliDept. of Applied Biology, University of Perugia, Borgo XX giugno, 74, 06121 Perugia (Italy)Apomixis is a challenging trait and offers unique opportunities for developing superior varietiessince it enables us to develop hybrids or genotypes that breed true regardless of heterozygosity.Apomixis would make it possible to fix the genotype of a superior plant variety bred for aparticular environment or market niche so that clonal seeds could be continuously and cheaplyproduced independent of pollination. If apomixis were well understood and harnessed, it couldbe exploited to indefinitely propagate superior hybrids or specific genotypes bearing complexgene sets. Until the gene(s) that promote and control apomixis are molecularly understood, thistrait can only be introgressed into agricultural crops through traditional breeding methods, mostof which are slow and laborious and require progeny tests for the selection of apomicticgenotypes after each round of backcrossingIn our previous work by applying the cDNA-AFLP transcriptional profiling technique weisolated messengers from developmental staged inflorescences of Poa pratensis L. In particular,more than two thousands transcript-derived fragments were visualized 179 of which weredifferentially expressed between apomictic and sexual genotypes. A major finding is that most ofthe genes expressed in florets displayed similar patterns during sexual and apomictic pathways.Of the about 8% of mRNAs differentially expressed between apomictic and sexual genotypes,the vast majority were attributable to genes with a differentiated temporal expression duringflowering. As few as 1.6% mRNAs specific to sexual or apomictic genotypes were found,providing that a highly conserved developmental program exist in embryos during zygoticembryogenesis and apomeiotic parthenogenesis. In particular one of these genes (APOSTART)was found to be flower-specific and showed a differentiated expression in reproductive tissuesbetween apomictic and sexual genotypes of P. pratensis. We developed a SCAR marker from anAPOSTART apomictic-specific allele and then tested for its potential use in MAS programs.APO-SCAR primer pair was first tested on an F 1 population of 68 individuals segregating for themode of reproduction and on its parental genotypes. The APO-SCAR pair of primers produced asingle amplification product of 225 bp, present in the apomictic paternal genotype but absent inthe maternal sexual plant.Project supported by: Fondazione Cassa di Risparmio di Perugia. Project 2008.021.498:Genomica comparativa e funzionale per caratterizzare i geni candidati al controllo dellemodificazioni del sistema riproduttivo.145
Effects of intraspecific competition on growth and seed yield of contrastingsulla genotypesG. Amato & D. GiambalvoDipartimento di Agronomia ambientale e territorialeUniversità di Palermo – Viale delle Scienze, 90128 Palermo, Italy.Email: amato@unipa.itAbstractSulla (Hedysarum coronarium L.) is a short-lived perennial legume native to the Mediterraneanbasin, where it is grown extensively as a 2-year forage crop. Seed is often obtained as asecondary product from second-year crops established to produce forage. Very little informationis available on seed yield capacity in the first year of the crop cycle and on the influence ofagronomical techniques on the reproductive process. The present study aimed to evaluate theeffect of intraspecific competition on dry matter accumulation (both in epigeic organs and intaproots) and seed production in the first year of a crop cycle for two contrasting genotypes. Twofield trials were carried out in a Mediterranean environment during the 2003/2004 and2004/2005 growing seasons. The experiment was set up as a split-plot design with fourreplications. The treatments were as follows: 1) genotype: Gangi or Resuttano (both originatedfrom inside Sicily but differed in terms of growth rate during the 2 years of the cycle); and 2)intraspecific competition realized by means of two plant densities: 100 and 600 plants m -2 . Theresults showed that in the first year of the cycle, the two landraces had very different behavior:Resuttano tended to accumulate C reserves in taproots, whereas Gangi used the photoassimilatesmainly for epigeic growth and the gamic reproduction process. As a result, Gangi producedabout 550 kg ha -1 seed, whereas Resuttano had a negligible seed yield. The increase inintraspecific competition increased above- and belowground biomass at the beginning offlowering irrespective of genotype but had no effect on crop growth or seed yield at maturity.IntroductionSulla (Hedysarum coronarium L., syn. Sulla coronaria [L.] Medik.) is a short-lived perenniallegume native to the Mediterranean basin (Talamucci, 1998), where it is grown extensively as a2-year forage crop for grazing and/or hay or silage production. The species plays a key role incereal-based systems of semi-arid regions, particularly in organic and low-input agriculture. It iscommonly used to enhance the productivity and sustainability of farming systems (e.g., to supplynitrogen and to maintain soil organic matter). Recently there has been a newfound interest insulla both in traditional and in nontraditional areas because of its excellent adaptability tomarginal and drought-prone environments (Borreani et al., 2003; Annichiarico et al., 2008).Moreover, it has several non-agricultural uses; for example, it is planted for soil protection(Watson, 1982) and revegetation of disturbed lands (Flores et al., 1997) as well as for honey146
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Effects of intraspecific competition on growth and seed yield of contrastingsulla genotypesG. Amato & D. GiambalvoDipartimento di Agronomia ambientale e territorialeUniversità di Palermo – Viale delle Scienze, 90128 Palermo, Italy.Email: amato@unipa.itAbstractSulla (Hedysarum coronarium L.) is a short-lived perennial legume native to the Mediterraneanbasin, where it is grown extensively as a 2-year forage crop. <strong>Seed</strong> is often obtained as asecondary product from second-year crops established to produce forage. Very little informationis available on seed yield capacity in the first year of the crop cycle and on the influence ofagronomical techniques on the reproductive process. The present study aimed to evaluate theeffect of intraspecific competition on dry matter accumulation (both in epigeic organs and intaproots) and seed production in the first year of a crop cycle for two contrasting genotypes. Twofield trials were carried out in a Mediterranean environment during the 2003/2004 and2004/2005 growing seasons. The experiment was set up as a split-plot design with fourreplications. The treatments were as follows: 1) genotype: Gangi or Resuttano (both originatedfrom inside Sicily but differed in terms of growth rate during the 2 years of the cycle); and 2)intraspecific competition realized by means of two plant densities: 100 and 600 plants m -2 . Theresults showed that in the first year of the cycle, the two landraces had very different behavior:Resuttano tended to accumulate C reserves in taproots, whereas Gangi used the photoassimilatesmainly for epigeic growth and the gamic reproduction process. As a result, Gangi producedabout 550 kg ha -1 seed, whereas Resuttano had a negligible seed yield. The increase inintraspecific competition increased above- and belowground biomass at the beginning offlowering irrespective of genotype but had no effect on crop growth or seed yield at maturity.IntroductionSulla (Hedysarum coronarium L., syn. Sulla coronaria [L.] Medik.) is a short-lived perenniallegume native to the Mediterranean basin (Talamucci, 1998), where it is grown extensively as a2-year forage crop for grazing and/or hay or silage production. The species plays a key role incereal-based systems of semi-arid regions, particularly in organic and low-input agriculture. It iscommonly used to enhance the productivity and sustainability of farming systems (e.g., to supplynitrogen and to maintain soil organic matter). Recently there has been a newfound interest insulla both in traditional and in nontraditional areas because of its excellent adaptability tomarginal and drought-prone environments (Borreani et al., 2003; Annichiarico et al., 2008).Moreover, it has several non-agricultural uses; for example, it is planted for soil protection(Watson, 1982) and revegetation of disturbed lands (Flores et al., 1997) as well as for honey146