Texas, USA 2010 - International Herbage Seed Group
Texas, USA 2010 - International Herbage Seed Group Texas, USA 2010 - International Herbage Seed Group
Stem rust in perennial ryegrass seed crops: epidemiological and geneticresearch at USDA-ARSW.F. PfenderUSDA-ARS NFSPRC3450 SW Campus WayCorvallis, Oregon 97331 USABill.Pfender@ars.usda.govAbstractIn production of perennial ryegrass seed in the Northwest USA, stem rust is the primary diseaseconstraint in terms of management cost and potential yield loss. The USDA-ARS researchprogram on this problem is focused on improved decision making for fungicide use, and tools forgenetic improvement. Data from 10 years of field and greenhouse research was used to constructan epidemiological model. This model is based on the effects of weather (temperature, leafwetness) on plant growth and pathogen activity, and includes the effects of two major fungicideclasses (triazoles and strobilurins) on disease development, particularly on the spread of diseasewithin a plant. The model is implemented as a decision aid on a publicly-accessible internet site.Users enter scouting data and select a location for weather data to be loaded into the model. Thewebsite displays the model results, including the relationship of modeled disease development toan action threshold. The decision aid has performed well in large-scale field demonstration plotsacross 10 location-years, providing an average economic advantage of $39 per acre (range: $0 to$140) compared to standard fungicide programs. In genetic research, a perennial ryegrassmapping population was constructed from a cross between resistant and susceptible parents. Theprogeny (193 individuals) were tested by inoculating with two different pathotypes of the stemrust fungus, in separate experiments. Using a genetic map of these parents constructed with SSRand RAD markers, QTL analyses were conducted. The goal of this genetic research is toproduce genetic markers for marker-assisted selection of stem rust resistance, as well as toprovide information about genomic organization of disease resistance in perennial ryegrass.Stem rust on perennial ryegrass and tall fescue in the Pacific Northwest USAIn perennial ryegrass (Lolium perenne) and tall fescue grown for seed, the economically mostimportant disease in major seed production areas of the United States is stem rust caused byPuccinia graminis subsp. graminicola (Welty & Azevedo, 1984) Perennial ryegrass seed yieldlosses up to 98% due to stem rust damage have been recorded (Pfender, 2009a). The disease isalso a significant production constraint in New Zealand, where yield losses of 35% weredocumented (Hampton, 1986). Since the 1980s, there has been a research program at the USDA-ARS Forage Seed Research Laboratory in Corvallis, OR, USA. Starting in the late 1990s theresearch focused on developing an epidemiological model that could be used in a decision113
support system to optimize fungicide use in disease management. Within the last few yearsanother component, stem rust resistance genetics in Lolium, has been added to the effort.Epidemiological research and fungicide decision support systemThe goal of the epidemiological work at USDA-ARS has been to quantify the effects ofenvironmental factors on disease development, and to summarize that knowledge in the form ofa mathematical model. The model is intended to depict the linked disease cycle components, andis driven by weather data that can be collected with automated weather stations in the field.Stem rust epidemic modelWe determined that the probability of infection, given the presence of stem rust inoculum, can becalculated from temperature and leaf wetness occurring during nighttime hours and in the fewhours after sunrise. If the leaves are wet and temperature is greater than 2C, the fungus sporescan germinate and grow. At cold temperatures, a long period of wetness is required for adequategrowth to cause infection, but the required wetness duration is shorter at warmer temperatures.The temperature X wetness duration must be adequate during both the dark period (for sporegermination) and the early morning period (for penetration into the plant). We derived anequation for calculating infection probability from overnight and morning weather data recordedin the field (Pfender, 2003). After the fungus has successfully penetrated the plant, some time isrequired for the infection to develop sufficiently to produce the next generation of spores. Theduration of this time period between infection and production of new spores (the latent period) isdetermined primarily by temperature. The latent period duration can be calculated byaccumulation of heat units (e.g. degree days) (Pfender, 2001). The processes of infection and thelatent period form the core of the stem rust epidemic model.An additional important aspect of stem rust development is the ability of the fungus to spreadfrom infections on the sheath to the stem or flower that is extending from within the infectedsheath (Pfender, 2004). This disease spread occurs because the pustule on the sheath isproducing spores on the inner sheath surface as well as the outer surface. The result is that asingle infection on the flag sheath, for example, can produce hundreds of infections on theinflorescence head and stem. Our observations indicate that up to 80% of the infections thatappear on ryegrass stem sections at the height of an epidemic are the result of this process. Theamount of disease due to this within-plant component is dependent not only on the frequency oflesions on sheaths, but also on the rate of extension of the internodes. Therefore the epidemicmodel includes a component for stem elongation (Pfender, 2006), which is driven by heat units.The full epidemic model includes infection probability, latent period and within-plant spread.The model works on a daily time step, and the amount of inoculum at the beginning of each dayis proportional to the amount of disease (sporulating pustules) at the end of the previous day.Although within-plant spread seems to be a general feature of stem rust in perennial ryegrass, wehave noted that varieties differ in the prominence of the sheath lesions that act as sources for the114
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support system to optimize fungicide use in disease management. Within the last few yearsanother component, stem rust resistance genetics in Lolium, has been added to the effort.Epidemiological research and fungicide decision support systemThe goal of the epidemiological work at USDA-ARS has been to quantify the effects ofenvironmental factors on disease development, and to summarize that knowledge in the form ofa mathematical model. The model is intended to depict the linked disease cycle components, andis driven by weather data that can be collected with automated weather stations in the field.Stem rust epidemic modelWe determined that the probability of infection, given the presence of stem rust inoculum, can becalculated from temperature and leaf wetness occurring during nighttime hours and in the fewhours after sunrise. If the leaves are wet and temperature is greater than 2C, the fungus sporescan germinate and grow. At cold temperatures, a long period of wetness is required for adequategrowth to cause infection, but the required wetness duration is shorter at warmer temperatures.The temperature X wetness duration must be adequate during both the dark period (for sporegermination) and the early morning period (for penetration into the plant). We derived anequation for calculating infection probability from overnight and morning weather data recordedin the field (Pfender, 2003). After the fungus has successfully penetrated the plant, some time isrequired for the infection to develop sufficiently to produce the next generation of spores. Theduration of this time period between infection and production of new spores (the latent period) isdetermined primarily by temperature. The latent period duration can be calculated byaccumulation of heat units (e.g. degree days) (Pfender, 2001). The processes of infection and thelatent period form the core of the stem rust epidemic model.An additional important aspect of stem rust development is the ability of the fungus to spreadfrom infections on the sheath to the stem or flower that is extending from within the infectedsheath (Pfender, 2004). This disease spread occurs because the pustule on the sheath isproducing spores on the inner sheath surface as well as the outer surface. The result is that asingle infection on the flag sheath, for example, can produce hundreds of infections on theinflorescence head and stem. Our observations indicate that up to 80% of the infections thatappear on ryegrass stem sections at the height of an epidemic are the result of this process. Theamount of disease due to this within-plant component is dependent not only on the frequency oflesions on sheaths, but also on the rate of extension of the internodes. Therefore the epidemicmodel includes a component for stem elongation (Pfender, 2006), which is driven by heat units.The full epidemic model includes infection probability, latent period and within-plant spread.The model works on a daily time step, and the amount of inoculum at the beginning of each dayis proportional to the amount of disease (sporulating pustules) at the end of the previous day.Although within-plant spread seems to be a general feature of stem rust in perennial ryegrass, wehave noted that varieties differ in the prominence of the sheath lesions that act as sources for the114