Contents - College of Medical and Dental Sciences - University of ...

More documents

Recommendations

Info

The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK Lytic Replication Abstract 37 NOVEL FUNCTIONS OF K-RTA AND K-BZIP AS SUMO-TARGETING LIGASES AND EPIGENETIC REGULATORS Hsing-Jien Kung, Pei-Ching Chang, Latricia Fitzgerald, Tom Ellison, Paul Luciw* and Yoshi Izumiya Dept. of Biochemistry and Molecular Medicine, *Dept of Pathology, UC Davis Cancer Center, Sacramento CA 95864 Abstract SUMO modification emerges as a major post-translational modification of viral and cellular proteins. Sumoylation in concert with histone methylation at H3K9 are hallmarks of heterochromatin. Like phospho-tyrosines, sumoylated lysines also serve as signals linking components of signal transduction. SUMO interacting motifs (SIM) have been identified in a number of cellular regulatory proteins including PML, DAXX and RNF4. RNF4, a SUMO-targeting ubiquitin ligase, preferentially facilitates the degradation of sumoylated proteins. Here we report the identification of K-Rta and K-bZIP as SUMO binding proteins, and have mapped the SIMs in these molecules. We have previously shown that K-bZIP behaves like a SUMO-ligase, via its ability to bind Ubc9 E2 SUMO conjugation enzyme (which is itself sumoylated). Studies from the labs of Hayward and Wood showed that K-Rta behaves like a ubiquitin ligase. We reported that K-Rta decreases the overall level of sumoylated proteins, and is counteracted by K-bZIP. We now show that K-Rta preferentially degrades sumoylated interacting protein. Taken in the present context, these data are consistent with the notion that K-bZIP is a SUMOtargeting SUMO ligase, and K-Rta, a SUMO-targeting Ubiquitin ligase. If true, they are the first SUMO-targeting viral ligases identified. The ability of K-Rta and K-bZIP to modulate the activity and stability of sumoylated proteins raises the possibility of their roles in the epigenetic regulation of viral latency. Data will be presented that JMJD2A, a H3K9me3 histone demethylase, is sumoylated and its demethylase activity is directly blocked by K-bZIP. Presenting author Email: yizumiya@ucdavis.edu 62
The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK Lytic Replication Abstract 38 X-BOX BINDING PROTEIN 1 DOES NOT INDUCE EBV LYTIC REPLICATION IN PRIMARY EFFUSION LYMPHOMA Imogen Yi-Chun Lai and Paul Kellam MRC Centre for Medical Molecular Virology, Department of Infection, University College London. 46 Cleveland Street, London W1T 4JF Abstract The plasma cell differentiation factor XBP-1 has been shown to transactivate the KSHV ORF50 promoter in primary effusion lymphoma (PEL) cells, leading to reactivation of KSHV from latency. PEL is a plasmablast lymphoma, arrested immediately before terminal differentiation into antibody secreting plasma cells. PEL are negative for the spliced transcriptional active form of XBP-1, namely XBP-1s. All PEL cells are infected with Karposi’s Sarcoma-associated Herpesvirus (KSHV), with about 75% also co-infected with Epstein - Barr virus (EBV). However little is known about the reactivation of EBV from latency in PEL cells. XBP-1s has been suggested to transactivate the immediate early gene BZLF-1 of EBV. Here we investigate the effect of different inducers of EBV lytic reactivation in PEL cells. By using Western blot, we show that XBP-1s does not induce the lytic reactivation of EBV defined by lack of expression of BZLF-1, despite inducing lytic reactivation of KSHV. Presenting author Email: imogen.lai@ucl.ac.uk 63
Page 1 and 2:
The 11 th International Workshop on
Page 3 and 4:
Scientific Programme Committee Davi
Page 5 and 6:
Page 7 and 8:
Travel Directions and Maps The 11 t
Page 9 and 10:
By rail The 11 th International Wor
Page 11 and 12: Agenda The 11 th International Work
Page 13 and 14: Wednesday, July 23 rd The 11 th Int
Page 15 and 16: Thursday, July 24 th The 11 th Inte
Page 17 and 18: Friday July 25 th The 11 th Interna
Page 19 and 20: The 11 th International Workshop on
Page 61: The 11 th International Workshop on
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Adam Grundhhoff Friedensallee 63 Ha
Page 119 and 120:
Dan Frampton Department of Infectio
Page 121 and 122:
Fang Cheng Vuolukiventie 1b g145 He
Page 123 and 124:
Keiji Ueda Dept.of Infectious Disea
Page 125 and 126:
Prof Juergen Haas Max-von-Pettenkof
Page 127 and 128:
Vladimir Majerciak 4817 36th Street
Page 129 and 130:
Page 131 and 132:
Page 133:
show all

Contents - College of Medical and Dental Sciences - University of ...

Create successful ePaper yourself

Delete template?

Save as template?