Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK<br />
Gene Expression I Abstract 29<br />
SCREENING FOR XINJIANG KS ASSOCIATED GENES AND THE STUDY ON THEIR<br />
MECHANISMS<br />
Lei YANG, Yan ZENG, Dong-Mei LI,Ling-ling XIAN,Xiao –Fei ZHOU,Xiao-Hua TAN,Jin<br />
HUANG,Feng LI,Jian-Xin XIE,Hui ZHANG,Sheng Wang,Xian-Dao LUO<br />
Key Laboratory <strong>of</strong> Xinjiang Endemic <strong>and</strong> Ethnic Disease, Shihezi <strong>University</strong>,Shihezi,<br />
XinJiang, 832002, China<br />
Abstract<br />
In China, classical KS mainly occurs in Uygur <strong>and</strong> Kazak ethic group in Xinjiang<br />
Autonomous Region. The occurrence <strong>and</strong> development <strong>of</strong> sarcoma is linked with the<br />
abnormality <strong>of</strong> genes. For comprehensive clarifying the pathogenesis <strong>of</strong> KS in Xinjiang,<br />
We screened the differentially expressed genes between KS’ tissues <strong>and</strong> normal skin<br />
tissues from an classical patient <strong>of</strong> Uygur by Suppression subtractive hybridization (SSH).<br />
We cloned <strong>and</strong> sequenced them <strong>and</strong> established differential expression cDNA library on<br />
the base <strong>of</strong> SSH.Then we enlarged the samples <strong>and</strong> selected 4 classic patients to do the<br />
further screening by human genome U133 plus 2.0 microarray (Affymetrix). According to<br />
the bioinformatics analysis,we got the congenerous differentially expressed genes<br />
nearly 80. The function <strong>of</strong> them are focused on signal transduction <strong>and</strong> correlated with<br />
apoptosis or tumor occurrence. These studies identified a large number <strong>of</strong> genes whose<br />
expression was altered in KS. we have verified some <strong>of</strong> these differentially expressed<br />
genes by Real-time PCR <strong>and</strong> Immunohistochemistry, it’s consistent with the SSH <strong>and</strong><br />
gene chip results.We have carried on some functional study on these genes,such as 14-<br />
3-3β, HO-1, Neuritin, EGFR, MMP14, LYN, et al. We detected the expression level <strong>of</strong> KS<br />
associated genes in BC-3 cells <strong>and</strong> Human umbilical vein endothelial cells to analyze if<br />
infection <strong>of</strong> HHV8 have effect on the expression <strong>of</strong> these genes .Most <strong>of</strong> these genes were<br />
related with the infection <strong>of</strong> HHV8 <strong>and</strong> participate in cell proliferation, apoptosis <strong>and</strong> this<br />
might show particular relation in the pathogenesis <strong>of</strong> KS.<br />
Presenting author Email: zengyan910@yahoo.com.cn<br />
53
Change language