Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
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The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK<br />
Poster Session Abstract P15<br />
KSHV DNA QUANTIFICATION IN MATCHED PLASMA AND PBMCs SAMPLES OF<br />
HIV+ PATIENTS WITH KSHV-RELATED LYMPHOPROLIFERATIVE DISEASES<br />
Rosamaria Tedeschi 1 , Alessia Marus 1 , Ettore Bidoli 2 , Cecilia Simonelli 3 , <strong>and</strong> Paolo De<br />
Paoli 1<br />
1 Microbiology-Immunology <strong>and</strong> Virology Unit; 2 Epidemiology Unit; 3 <strong>Medical</strong> Oncology<br />
Dept.; Centro di Riferimento Oncologico, IRCCS, Aviano, Italy<br />
Abstract<br />
Background: The quantitative evaluation <strong>of</strong> Kaposi’s sarcoma Herpesvirus (KSHV) viral<br />
load is not well described in the clinical management <strong>of</strong> KSHV-related lymphoproliferative<br />
diseases.<br />
Aim: To evaluate <strong>and</strong> to compare KSHV viral load in different blood compartments from<br />
HIV+ patients with Multicentric Castleman’s disease (MCD), Primary Effusion Lymphoma<br />
(PEL) <strong>and</strong> virus-associated solid lymphoma (SLY) <strong>and</strong> to establish which clinical sample<br />
would be preferable for KSHV DNA testing.<br />
Methods: We assessed KSHV DNA in plasma <strong>and</strong> PBMCs paired samples from 7 PEL, 8<br />
MCD, 2 SLY HIV+ patients at the diagnosis <strong>and</strong> during the course <strong>of</strong> the illness by using a<br />
real time PCR assay. EBV DNA, HIV RNA <strong>and</strong> CD4 cell counts were also measured.<br />
Results: KSHV viremia was always detectable at diagnosis among the three groups <strong>of</strong><br />
patients. KSHV DNA levels were correlated in matched pairs <strong>of</strong> samples at diagnosis <strong>and</strong><br />
also during follow-up (Spearman correlation coefficient: r=0.73; p