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The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK
Poster Session Abstract P11
KAPOSI’S SARCOMA-ASSOCIATED HERPESVIRUS (KSHV) INFECTED PRIMARY
EFFUSION LYMPHOMA CELLS SHOWS A DISTINCT GENE EXPRESSION PROFILE
FROM BURKITT LYMPHOMA CELLS EITHER WITH OR WITHOUT EPSTEIN-BARR
VIRUS AND THE OTHER T CELL LYMPHOBLASTIC LEUKEMIA CELLS
Keiji Ueda, Eriko Ohsaki, Tohru Suzuki and Masato Karayama
Dept. of Infectious Disease, Hamamatsu University School of Medicine,
1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
Abstract
Analysis on a gene expression profile using various kinds of normal or abnormal tissues,
including tumors, and cell lines etc., is very informative to know the nature of the cells,
which leads to further analyses and exploring new methods to treat diseased cells.
Though many reports have already published on gene expression profiles of KSHV
infected tissues, either in vitro or in vivo, we will report here gene expression profiles of
cultured cell lines; KSHV infected primary effusion lymphoma cell lines such as BC1, BC3,
BCBL1 and TY1, Burkitt cell lines such as BJAB, Ramos, which are not infected with
Epstein-Barr virus (EBV) and Daudi, Raji, Akaka, which are infected with EBV, and T
lymphoblastic cell lines as Sup T1, Molt 3 and Jurkat, and HIV-1 infected MT4.
What we found is that each categorized group of cell lines showed distinct gene
expression profiles and Burkitt cell lines infected with or without EBV showed a similar
profile. Since all PEL cell lines are infected with KSHV and BC1 is infected with both EBV
and KSHV, we can not conclude that KSHV infection governs the gene expression profile
but it is likely that EBV status does not affect the profile.
We are further studying how a typical gene is activated in the PEL cell lines and will
discuss about it.
Presenting author Email: kueda@hama-med.ac.jp
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