Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
Contents - College of Medical and Dental Sciences - University of ...
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The 11 th International Workshop on KSHV & Related Agents, Birmingham, UK<br />
Poster Session Abstract P4<br />
STUDIES ON THE ROLE OF MICROENVIRONMENT IN THE DEVELOPMENT OF<br />
PRIMARY EFFUSION LYMPHOMA<br />
Calabrò ML, 1 Gasperini P, 2 Di Gangi IM, 1 Indraccolo S, 1 Amadori A, 1 Chieco-Bianchi L. 2<br />
1 Immunology <strong>and</strong> Diagnostic Molecular Oncology, Istituto Oncologico Veneto, IRCCS,<br />
Padova; 2 Department <strong>of</strong> Oncology <strong>and</strong> Surgical <strong>Sciences</strong>, Oncology Section, <strong>University</strong> <strong>of</strong><br />
Padova, Italy.<br />
Abstract<br />
We used a SCID mouse model <strong>of</strong> primary effusion lymphoma (PEL) that mimics the<br />
aggressive course <strong>of</strong> this human KSHV-induced lymphoma to dissect the contribution <strong>of</strong><br />
the host microenvironment in PEL development. The activity <strong>of</strong> a murine, i.e. hostspecific,<br />
interferon (IFN)-α1-expressing lentiviral vector (mIFN-α1-LV) was compared to<br />
that <strong>of</strong> a hIFN-α2b-LV on the in vitro <strong>and</strong> in vivo growth <strong>of</strong> PEL cells. A control EGFP-LV<br />
was used in parallel. Lentiviral vectors efficiently delivered the transgene to PEL cells <strong>and</strong><br />
conferred long-term transgene expression both in vitro <strong>and</strong> in vivo. Treatment <strong>of</strong> PELinjected<br />
SCID mice with hIFN-α2b-LV was found to significantly prolong the survival in<br />
the majority <strong>of</strong> animals compared to control treatments, <strong>and</strong> was associated with a<br />
remarkable reduction in ascites formation. Interestingly, the delivery <strong>of</strong> a host-specific<br />
cytokine showed an anti-neoplastic activity comparable to that observed with the hIFNα2b-LV,<br />
thus suggesting that the specific targeting <strong>of</strong> the host microenvironment may<br />
impair PEL cell growth in vivo.<br />
Presenting author Email: lcalabro@unipd.it<br />
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