AGRONOMIJAS VÄSTIS - Latvijas LauksaimniecÄ«bas universitÄte

More documents

Recommendations

Info

AGRONOMIJAS VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 2008The comparison of sequence results of the both allele 1 and allele 2 (Fig. 2A and B)allowed us to read the sequences we have obtained for resting three animals (Fig. 2C) and to makeconclusion on heterozygote genotype of gene portion analyzed (Table 1, Fig. 2).Based on the analysis of both homozygote and heterozygote conditions of allele 2 wesuggest that SNP A 766 →T could be linked with MS motif in one linkage block.In our pilot study we demonstrated that MSTN gene MS polymorphism is represented inLatvian Blue cattle breed at least by two alleles of different repeated portion motifs andneighbouring SNPs polymorphisms. In set of 7 animals analyzed allele 1 was more frequent (6 and3 alleles for 3 homozygotes and 3 heterozygotes correspondingly) and allele 2 was revealed as lessfrequent ( 2 and 3 alleles for 1 homozygote and 3 heterozygotes). As animals were not selectedspecially for any traits we could suggest that the same allele presentation and distribution couldcharacterize Latvian Blue cattle breed in general. However, broad population study is necessary toprove all our suggestions.ConclusionsMSTN gene MS polymorphism is represented in Latvian Blue cattle breed at least by twoalleles of different repeated portion motifs and neighbouring SNPs polymorphisms.Allele 2 is different from the reported sequence (GeneBank: AB076403) variant not only inMS core motif but also in several SNPs including SNP A 766 →T in 5’ region from the MS repeatedportion.Longest allele 1 was more frequent and shortest allele 2 was revealed as less frequent. Asanimals were not selected specially for any traits we could suggest that the same allele presentationand distribution could characterize Latvian Blue cattle breed in general.AcknowledgementsAuthors are grateful to Latvian Ministry of Agriculture for financial support, contractworks No 120706/S386 and No 201006/C-266. We thank Ilva Poudziunas for assistance.References1. Charlier, C., Coppiters, W., Farnir, F., Grobet, L., Leroy, P., Michaux, C., Mni, M., Schwers, A.,Vanmanshoven, P., Hanset, R. (1995) The mh gene causing double muscling in cattle maps to bovinechromosome 2. Mamm. Genome 6, 788-792;2. De la Rosa-Reyna, X. F., Perez, M. A. R., Sifuentes-Rincon, A. M. (2005) Microsattelite polymorphismin intron 1 of the bovine myostatin gene. J. Appl. Genet., 47, 1–3;3. Grislis, Z. (2006). Zilās Govs Vidzemē. Biedrība Širnes saglabāšanas apvienība “Zilā Govs”. Jelgava,36.4. Grobet, L., Martin, L., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., Schoeberlein, A., Dunner, S.,Mienissier, J., Joulia-Ekaza, D., Cabello, G. (2006) Myostatin regulation of muscle development: Molecularbasis natural mutations, physiopathological aspects. Exp. cell research 312,2401-2414;5. Kambadur, R., Sharma, M., Smith, T. P., Bass, J. J. (1997) Mutations in myostatin (GDF8) in doublemuscledBelgian Blue and Piedmontese cattle. Genome Res. 7, 910-916;6. Karim, L., Coppieters, W., Grobet, L., Valentini, A., Georges, M. (2000) Convenient genotyping of sixmyostatin mutations causinf double-muscling in cattle a multiplex oligonucleotide ligation assay.Anim.Genet. 31, 396-399;7. Kassar-Duchaaussoy, L., Gayraud-Morel, B., Gomes, D., Rocancourt, D., Buckingham, M., Shinin, V.,Tajbakhsh, S. (2004) Mrf4 determines skeletal muscle identity in Myf5: MyoD double-mutant mice. Nature431, 466-471;8. Lewin, B. (2004) Genes VΙΙΙ.Pearson,Prentice Hall.9. McPherron, A., Lee, S. (1997) Double muscling in cattle due to mutations in the myostatin gene.Proceeding of the National Academy of Science of the United States of America, 94, 12457–12461;10. Rudnicki, M., Braun, T., Hinuma, S., Jaenisch, R. (1992) Inactivation of MyoD in mice leads to upregulationof the myogenic HLH gene Myf-5 and results in apparently normal muscle development. Cell 71,383-390;11. Sjakste, T., Zhuk, A. (2006) Novel haplotype description and structural background of the eventualfunctional significance of the barley beta-amylase gene intron rearrangements. Theor.Appl.Genet. 113, 1063-1079;270
AGRONOMIJAS VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 200812. Tajbakhsh, S., Rocancourt, D., Buckingham, M. (1996) Muscle progenitor cells failing to respond topositional cues adopt non-myogenic fates in myf-5 null mice. Nature 384, 266-270;13. Zhu, Z., Miller, B. (1997) MRF4 can substitute for myogenin during early stages of myogenesis. Dev.Dyn.209, 233-241.KOMBINĒTĀS DUBULTIEDARBĪBAS PIEDEVU IETEKME UZ ZĀLESFERMENTĀCIJU UN IEGŪTĀS SKĀBBARĪBAS KVALITĀTIINFLUENCE OF COMBINED CONSERVATION ADDITIVES ON GRASSFERMENTATION AND OBTAINED SILAGE QUALITYOšmane B., JemeĜjanovs A., Konošonoka I.Latvijas Lauksaimniecības Universitāte Biotehnoloăijas un Veterinārmedicīnas Zinātniskais Institūts „Sigra”,Zinātnes iela 1, Latvija, LV-2150, Fakss: +371 7976655, e-pasts: sigra@lis.lvLatvian University of Agriculture Research Institute of Biotechnology and Veterinary Medicine „Sigra”,Zinātnes iela 1, Latvia, LV-2150, Fax: +371 7976655, e-mail: sigra@lis.lvAbstractAerobic instability is a problem on many farms. Use of biological inoculant can improvesilage fermentation, but is not effective in protecting silage exposed to air. Aerobic deteriorationgenerates looses and reduces hygienic quality during the feed-out. A combination of inoculant andchemical treatment has the potential to enhance aerobic stability. The aim of experiment was toevaluate the effect of combined use of biological inoculant and chemical additives Na benzoate andK sorbate on fermentation quality and aerobic stability of red clover/galega silage during the feedout. The application of an additives Na benzoate and K sorbate enhanced lactic acid content,reduced pH and eliminated butyric acid fermentation in silage. When the silages were open to air, adiference in the pH and mould growth was significant. After 6 days exposure in open air pH levelon control variant (untreated silage) increased from 4,00 to 4,75, but of silage with inoculant andadditives pH level was stable. After 8 days, when silage was open, the untreated silage containedhigher count of moulds ( 1x10 -8 CFU g -1 – colony forming units), than silage with inoculant andadditives (0 CFU g -1 ). The treated silage quality and aerobic stability was not significantly (p>0,05)different between silage which open in 1, 3, 8 days. Silage with biological inoculant and Nabenzoate or K sorbate provides significantly (p
Page 1 and 2:
LATVIJAS LAUKSAIMNIECĪBAS UNIVERSI
Page 4 and 5:
AGRONOMIJAS VĒSTIS (Latvian Journa
Page 6 and 7:
Page 8 and 9:
Page 10:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
Page 210 and 211:
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
Page 218 and 219:
Page 220 and 221: AGRONOMIJAS VĒSTIS (Latvian Journa
show all

AGRONOMIJAS VÄSTIS - Latvijas LauksaimniecÄ«bas universitÄte

Create successful ePaper yourself

Delete template?

Save as template?

AGRONOMIJAS VÄSTIS - Latvijas LauksaimniecÄ«bas universitÄte