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AGRONOMIJAS VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 2008The dry matter content was inspected in two intervals (September 7 and November 3). Allthe species after the first interval (September 7) showed a significant influence from the inoculationwith lupine bacterial strains, with the exception of the species L. luteus variety ‘Juno’(Fig. 3). Theaverage increase of dry matter by 27 % was observed as the effect of inoculation. This regularityremained during all the periods of vegetation. At the end of the flowering period it was found thatthe species L. luteus had increased its dry matter content in the variants inoculated with thebacterial strains, the lowest was found in the control variant. Species L. angustifolius showedincreased dry matter content in the variant inoculated with nitragin, the control variant had thelowest values. For the species L. albus the increased dry matter content was found in the variantinoculated with the collection strains, the lowest values were in the control variant. For the speciesL. luteus and L. albus increasing dry matter content was obtained in the variants inoculated with thecollection strains. Processing the acquired data shows a significant correlation between lupinespecies and bacterial strains on plant dry matter.dry matter, %20181614121086420J0 J1 J2 W0 W1 W2 S0 S1 S2treatments and species7. sept.3. nov.RS 0.05 =1.45variety, strainFigure 3. The plant weight depending on the species and the treatment of the nitrogen fixingbacteria strain (J- ‘Juno’, W-‘Wat’, S-‘Sonet’ – varieties, 0- untreated variant, 1- treated withnitragin and 2- treated with Institute collection strain)The experiments showed the effect of inoculation on the protein content of lupine. Till theflowering phase there was no significant effect of the inoculation on the protein content, but furtherprotein accumulation was influenced by the nitrogen supply. The formed symbiotic system did notassure a sufficient nitrogen supply to the plants.The total protein amounts in dry matter were higher in the control variants (10 times highernitrogen dosages). Comparing the total amounts of protein in the lupines inoculated with nitrogenand the collection strains gave the following results: For for species L. luteus variety ‘Juno’ the bestsymbiotic system was formed with the collection strains. The total nitrogen content increased in thedry matter by 6% in comparison with the nitrigin treatment. For the species L. angustifolius variety‘Wat’ the best symbiotic system was formed with the collection strains, giving a increase of totalnitrogen in the dry matter by 5 %. For the species L. albus variety ‘Sonet’ the best symbioticsystem was formed with nitragin increasing the total nitogen in the dry matter by 10 % compared tothe collection strain.ConclusionsThe plant length depends on plant species. The smallest length was observed in the speciesL. angustifolius variety ‘Sonet’, the largest are the plants of the species L. luteus variety ‘Juno’.The inoculation of plant seeds promotes an increase in the plant length untill the phase ofbud formation. There are no significant differences between Rhizobium lupini strains.196
AGRONOMIJAS VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 2008The largest fresh and dry weight was observed for the L. albus variety ‘Wat’, but species L.angustifolius had the smallest fresh and dry weight.The largest dry matter content in plants untill the stage of bud formation was observed forinoculated plants.The effectiveness of Rhizobium lupini strains are species dependent.References1. Adomas B., Piotrowicz-Cieslak A, (2004) Amino acid composition , hemicellulose and soluble sugarscontent in narrow- leaves lupin seeds (Lupinus angustifolius L.) under the effect of reglone turbo 200SL, Electronic Journal of Polish Agricultural Universities, Agronomy, vol. 7, issue 2.http://www.ejpau.media.pl/series/volume7/issue2/agronomy/art-03.html.2. Kirichenko EV. Study of chemotaxis of nodule bacteria of lupin to organic substances Mikrobiol Z. 200567(3), 19-26.3. Korol, V. (2003) Enrichment of food products and combined feeds with lupin protein / MolochnayaPromyshlennost', (No. 5), 45-464. Metting B. F. (1993) Soil microbial ecology. New York, , 623.5. Ovalle C.; S. Urquiaga ;A. Del Pozo ;E. Zagal; S. Arredondo (2006). Nitrogen fixation in six foragelegumes in Mediterranean central Chile . Acta Agriculturae Scandinavica, Section B - Plant Soil Science,V.56, I. 4 , 277 – 283.6. Scliegel H. G. (1992) Allgemeine Microbiologie, 7 überarbeitete, Georg Thieme Verlag Stuttgart,NewJork, 408 – 416.7. Spaink H. P, C. A.Wijffelman, Elly Pees, R. J. H. Okker & B. J. J. Lugtenberg (1987). Rhizobiumnodulation gene nodD as a determinant of host specificity / Nature 328, 337 – 340.8. Stephens J.H.G., Rask H.M.( 2000), Inoculation production and formulation, Field Crop Research, 65,249-2589. Tate R. L. (1994) Soil Microbiology, John Wiley and Sons, Inc, 307 – 333.VĀRPU FUZARIOZES IZPLATĪBA UN MIKOTOKSĪNU RISKS ZIEMASKVIEŠU SĒJUMOS LATVIJĀOCCURRENCE OF THE FUSARIUM SPECIES AND THE RISK OFMYCOTOXINSASSOCIATED WITH HEAD BLIGHT IN WINTER WHEAT IN LATVIATreikale O., Priekule I., Pugačova J., Lazareva L.Latvijas Augu aizsardzības pētniecības centrs, Lielvārdes 36/38, Rīga, Latvija, LV-1006,phone: +371 6753764, e-mail: olga.treikale@laapc.lvAbstractDuring 2005-2006 the monitoring study was conducted by the Latvian Plant ProtectionResearch Centre. The incidence of Fusarial head blight (FHB) in winter wheat was investigated invarious Latvian regions. The distribution of the Fusarium species, isolated from samplesrepresenting production fields and variety testing trials with several winter wheat varieties andidentified by V.Bilai (1977) methods is described. The incidence of FHB varied from 2 to 35% in2005, from 1.66 to 25.2% in 2006 and there were no differences between regions. In 2005 - 5, in2006 - 6 the Fusarium species the caused FHB were identified. The most frequent were: F. poae,F. culmorum, F. gibbosum, besides F. avenaceum var. herbarum, F. sporotrichoides, F.moniliforme were found. Contamination of grain with mycotoxins was examined – in 2005 forDON, in 2006 – for DON, ZEN, T-2. Of 7 samples only 1 in 2005 and of 70 samples only 3 in2006 were contaminated with DON. The contamination level was very low – 24, 36, 36 and 28 µgkg -1 , which is below the level fixed in the EC Regulation 466/2001 – 1250 µg kg -1 .Key wordsFusarium species, incidence, frequency, mycotoxins, winter wheat197
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