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<strong>AGRONOMIJAS</strong> VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 2008The interest in biological nitrogen fixation is current in organic agriculture. Fixed nitrogen isincorporated in the protein and increases not only yield, but also the quality of the yield. Significantinfluence for increasing legumes yields are seed materials treatment with active nitrogen fixingbacteria strains before sowing. Bacteria of lupine Bradyrhizobium sp. (Lupinus), differ insymbiotic properties (competitiveness and nitrogen-fixing activity), to chemotaxis with respect tothe organic substances revealed in root exudates of lupine (amino acids, sugars, organic acids andtheir salts). (Kirichenko E., 2005)In agricultural practice nitrogen fixing bacterial treatment is economically profitable. Theeffectiveness of nitrogen fixing bacteria is expressed as an increase in the plant fresh mass and thetotal nitrogen amount in the dry matter.Lupine is one of the most popular legumes in the world. It is used as forage, green manureand in the last decade it has become more and more popular as a protein source. Lupine has almostthe same protein content as soya, but contains more carotene and cellulose, low levels of starch andhigh levels of polysaccharides and raffinose. However while soy is widely used in human nutrition,lupine is mainly used as an animal feed. The main shortcoming of lupine as a food material isconsidered to be its alkaloid content, which means that it must be subjected to processing toremove alkaloids before it can be used as a food material.(Korol, 2003; Adomas B and Piotrowicz-Cieslak A, 2004)The aim of this investigation was to test the symbiotic fixation effectivity of the bacterialstrains on different lupine species during their ontogenesis. The tasks were to determine plantlength, fresh weight, dry matter per plant, % of dry matter and the total nitrogen amount in the drymatter.Materials and MethodsThe experiments were carried out at the greenhouse of Latvia University of Agriculture,Faculty of Agriculture, Institute Soil and Plant Sciences. Three lupines species L. luteus variety‘Juno’, L. angustifolius variety ‘Sonet’ and L. albus variety ‘Wat’ were used in the vegetation potexperiment. The experiments were placed in five replicas in 5 L Mitcherlich type pots with washedriver sand, and fertilized with Kemira Grow How NPK 0-5-20 with microelements. Nitrogen wasadded as ammonium nitrate, 0.024 g per 1 kg of sand for inoculated plants and ten times more(0.24 g kg -1 ) for the control variant, were plants were not inoculated. In each pot 5 seeds weresowed. Lupine seeds were inoculates with a commercial preparation, nitragin, marketed by thecompany ‘Bioefekts’ (www.bioefekts.lv) and with the strain Rhizobium lupini from the collectionof the Institute of Soil and Plant Sciences.All vegetation pots were placed in a greenhouse with a day temperature of 20 – 25 °C and anight temperature over 12 °C. The experiment lasted from August 18 till December 26 when plantswere in the phase of the end of flowering and form pods. From October plants were illuminatedwith artificial light at morning and evening to obtain a total photoperiod 14 hours.Plant length, fresh mass, dry matter, % of dry matter, and total nitrogen amount in the drymatter were measured. Plants for analysis were taken at intervals: juvenile plants (September 7);bud formation (November 3) and at the end of flowering, pod formation (December 26). Thecontent of total nitrogen was determined with the Kjeldahl method at Latvia University ofAgriculture Analytical Laboratory for Agronomy Research. (ISO -5983-2 : 2005)Results and DiscussionThe activity of Rhizobium lupini strains was characterized by yield and accumulated nitrogenin the plants. Plant seed treatment with bacteria stimulated lupine germination and the plant growthat the early stages of development. At the first interval (till September 7) all species showed asignificant influence from the seed inoculation (Fig. 1.). This tendency remained till the secondinterval (till November 3). The best results were obtained with the institute bacterial strain. At theend of flowering period the species L. luteus had larger length in its untreated variant, the smallestlength were observed in the variant with the institute collection strain treatment. Species L.angustifolius had largest length in the variant inoculated with ‘Bioefekts’ nitragin and the smallestlength was observed in the untreated variant. In the species L. albus the major length was in the194
<strong>AGRONOMIJAS</strong> VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 2008variant inoculated with the collection strain and the smallest length was observed in the untreatedvariant. The main length increase of lupines was obtained at the intensive grown phase fromNovember 3 till December 26. In the end of the flowering period the best results were from thespecies L. luteus variety ‘Juno’ The smallest length was observed in the species L. angustifoliusvariety ‘Sonet’, from the species L. luteus the length decrease was 34 %. Data processing showedthat the plant length had a significant correlation to the lupine species and bacteria strain.6050plant lenght, cm40302010026.dec.3. nov.7. sept.ControlB1B2ControlB1B2ControlB1B2'Juno' 'Wat' 'Sonet'RS 0.05 =3.58variety, strainFigure 1. Plant length depending on the species and the treatment of the nitrogen fixing bacteriastrain (Control - untreated variant; B1 – treated with company ‘Bioefekts’ nitragin; B2 – treatedwith collection strain).For the lupine fresh weight gain observed at the end of the flowering period the best resultswere obtained for the species L. albus variety ‘Wat’. Species L. angustifolius had the smallest freshweight gain (Fig.2.). The effect of seed inoculation was observed till flowering. Plant fresh weightincreased by 20.9 %. Lupine treated with the Institute strain weighed 8 % more than the“Bioefekts” one. At the end of the experiment data analysis showed a significant interconnectionfor the varieties ‘Wat’and ‘Sonet’: lupines in the control variant (receiving higher nitrogen dosage)showed the lowest length, but higher fresh weight. Inoculated plants had longer length, but smallerfresh weight.3025weight, g2015107. sept.3. nov.26. dec.50J O J 1 J 2 W 0 W 1 W 2 S 0 S 1 S 2treatments and speciesRS 0.05 =0.91variety, strainFigure 2. Plant weight depending on the species and the treatment of the nitrogen fixing bacteriastrain (J- ‘Juno’, W-‘Wat’, S-‘Sonet’ – varieties, 0- untreated variant, 1- treated with nitragin and2- treated with Institute collection strain)195
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AGRONOMIJAS VÄSTIS - Latvijas LauksaimniecÄ«bas universitÄte