AGRONOMIJAS VĒSTIS - Latvijas Lauksaimniecības universitāte

AGRONOMIJAS VĒSTIS (Latvian Journal of Agronomy), No.10, LLU, 20084Oil content in seeds (g/100g)3,532,521,510,5200420050Lirina (st.) Vietējais 1 Vietējais 6 Riga Freis Rigaer B Blue di RigaFigure 3. Oil content in seeds of flax accessions of Latvian origin in 2004 and 2005Calli culture. Calli were obtained from both used explant types. Calli formation started twoweeks after the culture initiation. Stem segments and leaves developed homogeneous callistructures.For obtaining a higher number of plants-regenerants the influence of different antioxidantson calli growing and regenerating capacity was tested. After four weeks of growing on a shotformation medium with antioxidants calli induced from leaves were, in general, larger than stemcalli (Fig. 4). On calli formed from leaves, only the influence of the synthetic antioxidants ambioland AV-153 were positive. Ambiol and ascorbic acid have a positive influence on stem calli weight(calli weight was 47 mg heavier on the medium with ambiol and 82 mg heavier on the mediumwith ascorbic acid). Calli growing was dependent on the interaction of the genotype, explant type,and the used antioxidant.Calli weight (mg) '600550500450400350300250200150100500LeavesExplantsFigure 4. Weight of calli grown on shot formation medium with different antioxidants for 4 weeksdepending of explant typeStemControlAmbiolAV-153Ascorbic acidTriovitFlax seed ex.Formation of plants-regenerants started after five weeks of calli cultivation. Regenerationzones on a medium without antioxidants were formed only by stem calli. The use of antioxidantsambiol and AV-153 initiate the regeneration also from calli leaves (Fig. 5). The best results wereobtained when antioxidant ascorbic acid or flax seed extract were added to the medium: theregeneration capacity of stem calli was increased up to 5 times. For all genotypes shoot formationwas observed after 8 weeks of calli cultivation.115