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A Handbook of Rice Seedborne Fungi TW Mew and P ... - IRRI books

A Handbook of Rice Seedborne Fungi TW Mew and P ... - IRRI books

A Handbook of Rice Seedborne Fungi TW Mew and P ... - IRRI books

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ab→d→→→→f→→eg→→→→cFig. 30. Habit character <strong>of</strong> Fusarium moniliforme Sheld. on (a) whole seed (10X) <strong>and</strong> (b) awn portion (17X). (c)Mycelial growth <strong>of</strong> F. moniliforme showing false heads (49X). Photomicrograph <strong>of</strong> F. moniliforme showing (d)mycelia, (e) microconidiophores, (f) microconidia, <strong>and</strong> (g) macroconidia at 40X <strong>and</strong> stained with lactophenol blue.→Observed frequency (%)120100806040200SterilelemmasAwnPartitionbet. lemma<strong>and</strong> paleaSeed partEntireseedLemma/paleaonly orbothFig. 31. Observed frequency <strong>of</strong> Fusarium moniliformeoccurrence on the seed.Fig. 32. Plate cultures <strong>of</strong> Fusarium moniliforme Sheld.showing colony growths on PDA, PSA, <strong>and</strong> oatmeal agar(OA) incubated at ART, 21 °C, <strong>and</strong> 28 °C at 15 d afterinoculation.plate is zonated <strong>and</strong> white to dark purple. At 28 °Cunder alternating 12-h fluorescent light <strong>and</strong> 12-hdarkness, colonies grow moderately fast <strong>and</strong> attain a5.73-cm diam in 5 d. They are slightly zonated, floccosewith even margins, <strong>and</strong> white with light purplishcoloration at the center. The colony on the reverseside <strong>of</strong> the agar plate appears slightly zonated <strong>and</strong>dark purple <strong>and</strong> lighter outward.34

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