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Bonna-Agela Technologies

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Bonna-Agela Technologies— A Global Supplier forChromatography Solutions2011 Message From Bonna-Agela TechnologiesAs Bonna-Agela is poised to enter its fifth year with confidence and pride in itsinnovative separation, purification, and sample preparation products, we would liketo thank our many loyal customers for your continuous support and trust. With yoursupport and our effort in delivering the highest quality products to you, our companyhas grown remarkably. This has allowed us to expand our research and developmenteffort, and thus introduce more innovative products to better service your applicationneeds.In 2010, we had tremendous accomplishments: We cataloged over one thousanddifferent products. Our manufacturing and R&D operation were certified in compliancewith ISO 9001 and passed many quality audits by customers and distributors, includingVWR International. As a global wide company, we not only have our own internationalsales force but have also formed a marketing alliance with VWR. This will allow us toreach higher goals and to provide our customers with even better quality products andfaster service in the new year.Our 2011 mission statement and commitment:● Provide products with our innovative technologies at the best performanceto cost ratio.● Deliver products with guaranteed quality.● Provide global support with quick responses.

HPLC Columnsand MediaIntroductionHPLC Columns by FamilyNew ProductsUHPLC Technology and Column ProductsDurashell Columns ( C18, C8, Phenyl and NH 2 )Venusil HLPVenusil PAHVenusil XBP COOHVenusil PFP (USPL43)Chiral ColumnsInnoval ColumnsFeatured ProductsUnisol C18Unisol AmideVenusil ASB Series Columns (C1, C3, C8, C18 and Phenyl)Optimix Family of HPLC ColumnsStandard ProductsVenusil Family of HPLC ColumnsPromosil Family of HPLC ColumnsSelected Solutions for Popular ApplicationsOrdering Information by Type of Stationary PhasesReversed Phase ColumnsNormal Phase ColumnsIon-exchange ColumnsMixed Phase ColumnsSize Exclusion Columns (Silica Based)Chiral ColumnsPolymer Phase ColumnsGuard Cartridge HolderHPLC Accessories and SuppliesHPLC Column Selection GuideApplicationsAppendix001001001001003005006007007009012013013018022024027027040042053053057059060061061062065066068073092

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYIntroductionAs one of the emerging leaders in chromatography technology, Bonna-Agela Technologies proudly present five series of the featuredHPLC columns developed with our latest cutting-edge technologies. These products exhibit superior performance and are designed tomeet the ever-increasing demands of our customers.1.Unisol C18 - A type of true universal reversed phase column for separation of a broad spectrum of molecules such as acidic, basicand neutral compounds. It has a superior performance to other branded C18 phases in almost every aspects.2.Unisol Amide - It has a unique mechanism to separate molecules of different polarities. It can be used as either a reversed-phaseor normal phase column. It outperforms any other columns on the market in the HILIC category. When it is used as a HILIC column,it delivers desired retention for very polar compounds.3.Optimix columns – A class of columns provides optimized selectivity and balanced retention for hydrophilic and hydrophobiccompounds. It delivers the extension of retaining polar compounds and non-excessive retention of strong hydrophobic molecules.4.Durashell columns - A class of columns that can be used in a wide pH range of 1.0-12.0.5.Venusil ASB columns – This class of columns is tailored for acidic (pH=0.8) operation and LC-MS applications.HPLC Columns by Family001HPLC ColumnsNEW Products!UHPLC Technology and Column ProductsIn recent ultra-performance HPLC (UHPLC), sub-2μm particles are utilized to achieve higher efficiency and resolution and thus to result inimproved sensitivity. Due to the limit of back-pressure, conventional HPLC systems are not sufficient to drive the column packed with suchsmall particles. However, Bonna-Aglea UHPLC columns packed with 2 and 2.5μm particles of narrow size distribution minimize system backpressure,and allow application in conventional HPLC systems with comparable performance to UHPLC.The UHPLC packing material manufactured by Bonna-Agela is based on a new high purity spherical silica. A proprietary technology of surfacemodification and bonding process renders outstanding properties of the stationary phase with uniform surface and consistent chemistry on thespherical silica. As a result of this technology, interactions between polar analytes and silica surface are reduced significantly and subsequentlysymmetry for very basic compounds is maximized. A series of unique columns was developed to meeting the demanding requirements foranalysis of highly polar compounds. Lot-to-lot reproducibility is endured by a stable bonding or end-capping process.The UHPLC material is packed in newly designed column housings of extremely low void volume, which tolerate back-pressure up to 1000 baror 15000 psi.UHP AQ C18Characteristics: Metal Impurity

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersMain Features:● Greatly improved peak shape for basic compounds;● 100% water compatibility;● Low pH stability: stable at as low as pH=1.5;● Plate Count: 200000/m (2μm);● Tailing Factor: 0.98-1.12;● Pressure resistance: 1000bar (15000psi);UHP ASB C18Characteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYDurashell Columns(C18, C8, Phenyl and NH 2 )Bonna-Agela’s Durashell column has a pH range of 1.5-12.0. Different from the current marketed products that also can be used at highpH, Durashell’s stationary phase is made with a dentrimer technology followed by molecular modifications. Bonna-Agela’s technologyproduces a strong hydrophobic protection layer over the silica surface allowing the columns to be used at extremely high and low pH.This technology also reduces excessive hydrophobic interactions between the stationary phase and the compounds, and yet maintainsgreat interfacial kinetics for high efficiency. A line of stationary phases includes C18, C8, Phenyl and NH 2 .Characteristics: Metal Impurity

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersOrdering InformationSurface Area: 380m 2 /g, Pore Size: 100ÅDurashell RP: Surface Area: 200m 2 /g, Pore Size: 150ÅTypeParticle size(µm)Dimension(mm)Durashell C18 Durashell Phenyl Durashell NH 2 Durashell C8Durashell RPFast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysisAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativeSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativePreparativePreparative3333333335555555555555555555551010101010101010101010102.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250DC930302-0DC930502-0DC931002-0DC931502-0DC930102-0DC930102-0SDC930505-0DC931005-0DC931505-0DC950302-0DC950502-0DC951002-0DC951502-0DC950505-0DC951005-0DC951505-0DC952005-0DC952505-0DC950105-0DC950105-0SDC951510-0DC952510-0DC950520-0DC951520-0DC952520-0DC950110-0SDC950120-0DC951030-0DC951530-0DC952530-0DC901510-0DC902510-0DC900520-0DC901520-0DC902520-0DC900110-0SDC900120-0DC901030-0DC901530-0DC902530-0DC901550-0DC902550-0DC630302-0DC630502-0DC631002-0DC631502-0DC630102-0DC630102-0SDC630505-0DC631005-0DC631505-0DC650302-0DC650502-0DC651002-0DC651502-0DC650505-0DC651005-0DC651505-0DC652005-0DC652505-0DC650105-0DC650105-0SDC651510-0DC652510-0DC650520-0DC651520-0DC652520-0DC650110-0SDC650120-0DC651030-0DC651530-0DC652530-0DC601510-0DC602510-0DC600520-0DC601520-0DC602520-0DC600110-0SDC600120-0DC601030-0DC601530-0DC602530-0DC601550-0DC602550-0DN830302-0DN830502-0DN831002-0DN831502-0DN830102-0DN830102-0SDN830505-0DN831005-0DN831505-0DN850302-0DN850502-0DN851002-0DN851502-0DN850505-0DN851005-0DN851505-0DN852005-0DN852505-0DN850105-0DN850105-0SDN851510-0DN852510-0DN850520-0DN851520-0DN852520-0DN850110-0SDN850120-0DN851030-0DN851530-0DN852530-0DN801510-0DN802510-0DN800520-0DN801520-0DN802520-0DN800110-0SDN800120-0DN801030-0DN801530-0DN802530-0DN801550-0DN802550-0DC830302-0DC830502-0DC831002-0DC831502-0DC830102-0DC830102-0SDC830505-0DC831005-0DC831505-0DC850302-0DC850502-0DC851002-0DC851502-0DC850505-0DC851005-0DC851505-0DC852005-0DC852505-0DC850105-0DC850105-0SDC851510-0DC852510-0DC850520-0DC851520-0DC852520-0DC850110-0SDC850120-0DC851030-0DC851530-0DC852530-0DC801510-0DC802510-0DC800520-0DC801520-0DC802520-0DC800110-0SDC800120-0DC801030-0DC801530-0DC802530-0DC801550-0DC802550-0DS930302-0DS930502-0DS931002-0DS931502-0DS930102-0DS930102-0SDS930505-0DS931005-0DS931505-0DS950302-0DS950502-0DS951002-0DS951502-0DS950505-0DS951005-0DS951505-0DS952005-0DS952505-0DS950105-0DS950105-0SDS951510-0DS952510-0DS950520-0DS951520-0DS952520-0DS950110-0SDS950120-0DS951030-0DS951530-0DS952530-0DS901510-0DS902510-0DS900520-0DS901520-0DS902520-0DS900110-0SDS900120-0DS901030-0DS901530-0DS902530-0DS901550-0DS902550-0004HPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil HLPVenusil HLP columns are reversed-phase columns based on Bonna-Agela's amide technology. The stationary phase is made from highpurity spherical silica particles bonded with alkyl chain molecules and embedded amide polar group that literally capped the silica'sresidual silanols from interacting with highly basic analytes. It can be used to replace Waters Symmetry Shield RP18 and Agilent BonusRP.Characteristics: Metal Impurity

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersOrdering InformationSurface Area: 380m 2 /g, Pore Size: 100ÅTypeParticle size (µm)Dimension (mm)Venusil HLPAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCG55555554.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pkVHL950505-0VHL951005-0VHL951505-0VHL952005-0VHL952505-0VHL950105-0VHL950105-0SVenusil PAHVenusil PAH column is a polymerically bonded C18 column. It is recommended for the separation of PAHs and steric isomers of thearomatic compounds. PAHs are considered as priority pollutants and the analysis of these potentially carcinogenic compounds in water,soil and food is of major importance.Characteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil XBP COOHVenusil XBP COOH columns are made of high purity spherical silica particle, bonded with carboxylic acid functional group. They can beused for separation of basic, water-soluble compounds and bio-molecules.Characteristics: Metal Impurity

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersDopamine and Its MetabolinOHHOHONHHOOHNH 2DopamineIsoprenalineIntens.mAU6005101409 .D: UV Chromatogram, 280 nm402000 5 10 15 20 25 30 35 40 Ti me [mi n]Sample: Dopamine Isoprenaline SoprenalineColumn: Venusil PFP, 4.6×250mm, 5μmPart No.: VF952505-0Mobile Phase: MeOH: CH 3 COOH /CH 3 COONH 4Buffer (pH 4.5) =15:85Detection: UV 280nmFlow Rate: 0.8mL/minOrdering InformationSurface Area: 320m 2 /g, Pore Size: 120ÅTypeAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGParticle size (µm)5555555Dimension (mm)4.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pkVenusil PFPVF950505-0VF951005-0VF951505-0VF952005-0VF952505-0VF950105-0VF950105-0S008HPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge Holder

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYChiral ColumnsNormal Phase ColumnNormal phase chiral columns from Bonna-Agela Technologies are based on modified celluloses and starches. This type of chiral columnsrepresents the most effective means of analyzing chiral compounds and obtaining pure enantiomers, i.e., to levels of >99% enantiomeric excess.Main Festures:● Hydrogen bonding, π-π and the “embedded” composite integrated mechanism● Chiral AD-H and OD-H columns are available for 80% chiral compounds● Wide selectivity and high loading capacityChiral AD-HOverlayed Radical: Amylose - (3,5-dimethylphenyl carbamate)Separation Scale: Amide, Aromatic, carbonyl-group, nitro-group, sulfonyl-group, cyano-group, hydroxide radical, amine and carboxylicacid compoundsExample:009HPLC ColumnsNOL-val PMBNNNONHOPMBNONN ND-val PMBONHOPMB1: L-val PMB; 2: D-val PMBColumn: Chiral AD-H, 4.6×250mm, 5µm, 1000ÅMobile Phase: Hexane:Isopropanol=80:20Detection: 254nmFlow Rate: 1.0mL/minTemperature: 25℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersChiral OD-HOverlayed Radical: Cellulose - (3,5 - dimethylphenyl carbamate)Separation Scale: a phase suitable for the separation of beta-blockers and steroids, such as DHA, chlorine heart acyl amines, flavanones,metoprolol, etcExample:Column: Chiral OD-H, 4.6×250mm, 5µm, 1000ÅMobile Phase: Hexane:Isopropanol=98:2Flow Rate: 1.0mL/minDetection: 220nmTemperature: 25℃Chiral OJ-HOverlayed Radical: Cellulose - [4 - methyl benzoate]Separation Scale: the Chiral OJ-H column is used for the separation of carbonyl, amido, aryl, nitro, cyano ,sulfonyl, hydroxyl, amine andcarboxylic acid compounds.Example:010HPLC ColumnsOOHNOHNOHNOHNOOOVWD1 A, Wavelength=210 nm (ZJZ\10010605.D)Norm.3001250200150100500-500 5 10 15 20 25 30 35 40 452minColumn: Chiral OJ-H, 4.6×250mm, 5µm,1000ÅMobile Phase: Hexane:Isopropanol=93:7Flow Rate: 1.0mL/minDetection: 210nmTemperature: 30℃

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYChemical Bonded ColumnChiral Amide-1Chiral Amide-1 is packed with 5μm, 300Å silica bonded with a chiral compound whose structure is demonstrated as below:Consider this column as the first option to separate amide chiral compounds:● Can be used as reversed phase or normal phase● Unique selectivity and high resolution● More stable and longer column lifetimeSiCH 2 CH 2 CH 2 HNCOOOH 3CNExample: N-(3,5-Dinitrobenzoyl)-DL-leucineN011HPLC ColumnsColumn: Chiral Amide-1, 5μm, 300Å, 4.6×150mmMobile Phase: Methonal/10mM KH 2 PO 4 (pH=6.86)+0.5mMTetrabutyl ammonium hydroxide=30/70Flow Rate: 1.0mL/minDetection: UV254nmInjection: 5μLOrdering InformationTypeParticle size (µm)Dimension (mm)Chiral AD-HChiral OD-HChiral OJ-HChiral Amide-1AnalyticalAnalyticalPreparativePreparative55554.6×1504.6×25021.2×25030×250AD951505-0AD952505-0AD952520-0AD952530-0OD951505-0OD952505-0OD952520-0OD952530-0OJ951505-0OJ952505-0OJ952520-0OJ952530-0AM951505-1AM952505-1AM952520-1AM952530-1

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersInnoval ColumnsInnoval column is made of the purity silica particle' to 'high purity silica that is prepared by the ultra pure aggregation of silica sols. Thisclass of columns from Bonna-Agela shows excellent reproducibility and high efficiency. A variety of function groups is bonded to thepacking for broad applications.Characteristics: Pore Size 100Å; Available Particle Size: 5µm, Spectific: Surface Area: 240m 2 /gBalance Retention for Hydrophilic and Hydrophobic CompoundsInnoval SilicaSample: toluene, nitrobenzene, o-nitroaniline, m-nitroaniline and p-nitroanilineMobile Phase: Isooctane:ethanol:water=85:15:0.3Flow Rate: 1.00mL/minTemperature: room temperatureDetector: UV 254nmVenusil XBP Silica012HPLC ColumnsOrdering InformationSurface Area: 240m 2 /g, Pore Size: 100ÅTypeParticle size (µm)Dimension (mm)Innoval SilicaInnoval C18AnalyticalAnalyticalSemi-preparativeSemi-preparativePreparativePreparativePreparativePreparativePreparativePreparative55555555554.6×1504.6×25010×15010×25021.2×5021.2×15021.2×25030×10030×15030×250ISi 951505-0ISi 952505-0ISi 951510-0ISi 952510-0ISi 950520-0ISi 951520-0ISi 952520-0ISi 951030-0ISi 951530-0ISi 952530-0IX951505-0IX952505-0IX951510-0IX952510-0IX950520-0IX951520-0IX952520-0IX951030-0IX951530-0IX952530-0

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersGood Retention for Polar CompoundsMobile Phase: A: Water (Contain 0.01mM NaH 2 PO 4 and0.01mM Citric Acid, pH=4.5);B: ACN; A:B=95:5Sample: Cytosine, Uracil, GuanineTemperature: 35℃Detection: 254nmBalanced Retention for Hydrophilic and Hydrophobic CompoundsmAU500400300200100VWD1 A, Wavelength=254 nm (HLX\08021110.D)3.217 1.6416.4978.23314.13731.06148.842014HPLC Columns00 10 20 30 40 50 60 minmAU1VWD1 A, Wavelength=254 nm (MSW\08013140.D)4003002001003.5837.5309.13016.62100 10 20 30 40 50 60 minmAUVWD1 A, Wavelength=254 nm (MSW\08020100.D)400300200100013.0065.6166.732 7.42114.0120 10 20 30 40 50 6037.59958.32260.339minMobile Phase: 35% 20mM KH 2 PO 4 pH=7.0, 65% MethanolTemperature: 23℃Flow Rate: 1mL/minDetection: UV 254nmSample: UracilPropranolol hydrochlorideButyl PhthalateDimethyl PhthalateNaphthaleneAcenaphtheneAmitriptyline

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYExtremely Low Metal EffectsUnisol C18 5μm, 4.6mm×150mmBrand W, AQ Column 5μm, 4.6mm×150mmmAU40303.0084.084TF=1.31mAU5040302.2752.889TF=1.4020201010000 1 2 3 4 5 6 7min0 1 2 3 4 5 6minBrand H, C18 Column 5μm, 4.6mm×150mmBrand Z,C18 Column 5μm, 4.6mm×150mmmAU4035302.4503.471TF=1.69mAU25203.944TF=2.88252015151051055.93400 1 2 3 4 5 6 7min0 2 4 6 8 10min015HPLC ColumnsMobile Phase: A:Water; B:Methanol; A:B=65:35Flow Rate: 1mL/minTemperature: 25℃Detection: UV 230nmSample: 2,3-Dihydroxynaphthalene+2,7-DihydroxynaphthaleneGood Tolerance of Directly Injected Biological MatrixmAU60504030Fresh ColunmPlates = 322420100-100 1 2 3 4 5 6 7 minmAU60504030After 500 sample injectionsPlates = 281020100-100 1 2 3 4 5 6 7 minmAU60After column washing and re-equlibrium in50403020100% water (0.1% formic acid)Plates = 3045100-100 1 2 3 4 5 6 7 minSample: uridine in calf blood plasma (diluted 20 times with0.1% formic acid)Injection: 1μl (not be cleaned up with SPE)Column: Unisol C18, 2.1×50mm, 5μmTemperature: 30℃Flow Rate: 0.3mL/minMobile Phase: 0.1% formic acid solution (100% aqueous phase)Run Time: 8minAfter 500 injection, wash with methanol/0.1% formic acidsolution=50/50 for 30mins,column temperature 40℃rinse with 95% MeOH for 30mins, column temperature 45℃ .

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersEnable Methods to be Compatible with LC-MSmAU250200150100Analysis of Quaternary Ammonium Alkaloids from Copitidis witha Non-Volatile Ion Pair Reagent Using Brand Z C18 Column.500051 01 52 02 53 03 54 0minMobile Phase: ACN:0.05 mol/L KH 2 PO 4 Aq.(40:60, Contain 0.015 mol/L SDS)0.250.200.150.100.05Analysis of Quaternary Ammonium Alkaloids from Copitidiswithout a Non-Volatile Ion Pair Reagent Using Unisol C18Column .0.000.005.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00Mobile Phase: Water+0.2% TFA:acetonitrile=75:25016Compatibility with 100% Aqueous Mobile PhasemAU20 Unisol C18 , initial1000 2 4 6 8 10 12 14 16 18 minHPLC ColumnsmAU2010Unisol C18after 4 hours00 2 4 6 8 10 12 14 16 18 minmAU2010Luna C18 initial00 2 4 6 8 10 12 14 16 18 minmAU200100Luna C18 after 4 hours00 2 4 6 8 10 12 14 16 18 minmAU200100Brand K C8 initial00 2 4 6 8 10 12 14 16 18 minmAU400200Brand K after 4 hours00 2 4 6 8 10 12 14 16 18 minSample: UridineColumn: 4.6x150mm, 5μmMobile Phase: 100% waterFlow Rate: 1mL/min; the flow was stopped for 5 minutesduring the testing period for each columnTemperature: 30℃

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYGreater Volume Loading Capacity Than Other BrandsmAU20015010050Unisol C18,Plates=913700 1 2 3 4 5mAUmin2001501005000 1 2 3 4 5mAU20015010050Brand A-C18,Plates=202900 1 2 3 4 5minBrand Z-C18,Plates=2118minLarge Volume Injection (Injection volume=100μL)Sample: 4-methoxybenzenesulfon amide,10μg/mL in MeOH/Water (1:1)Column: 4.6x150mm, 5μmMobile Phase: MeOH/WaterFlow Rate: 1mL/minTemperature: 30℃017HPLC ColumnsStability of Unisol C18 at pH=1.5mAU403020100After 4 hour s0 1 2 3 4 5 6 7 8 9 minmAU40After 500 hour s30201000 1 2 3 4 5 6 7 8 9 minSample: Uracil, Doxepin, Amitriptyline and Toluene.Column: Unisol C18, 4.6x150mm, 5μmMobile Phase: TFA (0.25%, pH=1.5) in 60% acetonitrileFlow Rate: 1mL/ minTemperature: 40℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersPerfect Peak Symmetry and Good Stability at Mid-pH for Basic CompoundsmAU4035302520151052.0802.3204.0686.9619.77514.276Initial00 5 10 15 20 25 30 minmAU40After 150 hours353025201510500 5 10 15 20 25 30 min2.0402.3114.3376.0909.96514.271Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline and TrimipramineColumn: Unisol C18, 4.6×250mm, 5μmMobile Phase: 0.01M sodium phosphate:ACN=25:75, pH=7.0Flow Rate: 1.3mL/min.Temperature: 30℃018Unisol Amide (HILIC)Unisol Amide stationary phase is made from high purity spherical silica particles bonded with a neutral hydrophilic amide group. It can beused for normal phase, reversed phase, or hydrophilic interaction HPLC. The Unisol Amide (NP) column is a good replacement for NH 2 andsilica columns that are currently marketed as HILIC. Compared with traditional silica and NH 2 columns, the Unisol Amide columns have betterreproducibility and column lifetime. It is especially useful for the separation of strong hydrophilic compounds, whether they are acidic, basic orneutral. It is compatible with aqueous mobile phases in the pH range of 2.0-8.0. Unisol Amide presents unique selectivity for many moleculesbecause of its difference in separation mechanism from most of the conventional stationary phases.HPLC ColumnsCharacteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYSeparation of ValidamycinAu0.120.100.08CH 2 OHHOOHOHNHOHHOH 2 COHO OCH 2 OHHOOH0.06OH0.040.020.00-0.022.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 minMobile Phase: A: 0.1% TFA in Water; B: AcetonitrileGradient: 40% A to 85% A in 30 minFlow Rate: 1mL/minTemperature: 25℃Detection: UV 210nmSample: ValidamycinAu0.200.180.160.140.120.10019HPLC Columns0.080.060.040.020.00-0.020.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 minSeparation of Shikimic AcidMobile Phase: A:0.005M NaH 2 PO 4 (pH=7.0);B:MeOH; A:B=98:2Flow Rate: 1mL/minTemperature: 25℃Detection: UV 210nmSample: ValidamycinDetector(UV), Channel 1, Start Wavelength 210nm2.9178.108Column: Unisol Amide, 5μm, 4.6mm×150mmPart No.: UA951505-0Mobile Phase: A:1% Fomic Acid in Water; B: AcetonitrileGradient: 60% A to 90% A in 20 minFlow Rate: 1mL/minTemperature: 25℃Detection: UV 210nmSample: Shikimic Acid

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersWater-soluble VitaminsUnisol Amide, 5μm, 4.6mm×150mmPart No.: UA951505-0Brand W, NH 2 Column, 5μm, 4.6×150mmD AD 1 A, S ig =2 80 ,4 R ef =3 60 ,100 ( AG EL A\ 070 1300 0. D)mA U50 01.9572.300D AD 1 A, S ig = 280 ,4 R ef =3 60 ,100 ( AG EL A\ 07021 10 9. D)mA U25 01.6581.7684.67940 020 030 015 020 03.39210 010 05.573503.573000 1 2 3 4 5 6mi n0 1 2 3 4 5 6mi nBrand W Silica Column,5μm,4.6×150mmD AD 1 A, S ig =2 80 ,4 R ef =3 60 ,1 00 ( AGEL A\ 07 02 11 14 .D )mA U20 03.2083.4873.83515 0Mobile Phase: A:0.1% TFA in Water; B:0.1% TFA in Acetonitrile10 0A:B=90:10Flow Rate: 1mL/min5000 1 2 3 4 5 6Ordering InformationSurface Area: 410m 2 /g, Pore Size: 100Å4.404mi nTemperature: 30℃Detection: UV 280nmSample: VB1+VB6+VC+VB2020HPLC ColumnsTypeFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysisFast analysisFast analysisFast analysisParticle Size(µm) Dimension (mm) Unisol C18 Unisol Amide(HILIC)2.52.52.52.52.52.52.52.53333333333332.1×302.1×502.1×1003.0×303.0×503.0×1004.6×504.6×1002.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk3.0×303.0×503.0×1004.6×504.6×1004.6×150UO920302-0UO920502-0UO921002-0UO920303-0UO920503-0UO921003-0UO920505-0UO921005-0UO930302-0UO930502-0UO931002-0UO931502-0UO930102-0UO930102-0SUO930303-0UO930503-0UO931003-0UO930505-0UO931005-0UO931505-0VH930302-0VH930502-0VH931002-0VH931502-0VH930102-0VH930102-0SVH930303-0VH930503-0VH931003-0VH930505-0VH931005-0VH931505-0

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYSurface Area: 410m 2 /g, Pore Size: 100Å021HPLC ColumnsTypeAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativeSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativePreparativePreparativeParticle Size(µm) Dimension (mm)Unisol C18 Unisol Amide(HILIC)555555555555555555555551010101010101010101010102.1×302.1×502.1×1002.1×1503.0×303.0×503.0×1004.6×504.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250UO950302-0UO950502-0UO951002-0UO951502-0UO950303-0UO950503-0UO951003-0UO950505-0UO951005-0UO951505-0UO952505-0UO950105-0UO950105-0SUO951510-0UO952510-0UO950520-0UO951520-0UO952520-0UO950110-0SUO950120-0UO951030-0UO951530-0UO952530-0UO901510-0UO902510-0UO900520-0UO901520-0UO902520-0UO900110-0SUO900120-0UO901030-0UO901530-0UO902530-0UO901550-0UO902550-0VH950302-0VH950502-0VH951002-0VH951502-0VH950303-0VH950503-0VH951003-0VH950505-0VH951005-0VH951505-0VH952505-0VH950105-0VH950105-0SVH951510-0VH952510-0VH950520-0VH951520-0VH952520-0VH950110-0SVH950120-0VH951030-0VH951530-0VH952530-0VH901510-0VH902510-0VH900520-0VH901520-0VH902520-0VH900110-0SVH900120-0VH901030-0VH901530-0VH902530-0VH901550-0VH902550-0G: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersVenusil ASB Series Columns (C1, C3, C8, C18 and phenyl)The Venusil ASB series columns are specially designed for the separation of polar compounds from low (extremely stable at pH=1.0)to medium pH. The stationary phase is bonded with unique bulky silanes that sterically protect the siloxane bond. We offer a line ofbonding chemistry of C1, C3, C8, C18 or Phenyl groups presenting a broad selection of different polarity for various applications. Thiscolumn can be used to replace Zorbax SB.Characteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYOrdering InformationSurface Area: 200m 2 /g, Pore Size: 150ÅTypeParticle(μm)Dimension(mm)2.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250VenusilASB C18VenusilASB C8VenusilASB PhenylVenusilASB C3VenusilASB C1023HPLC ColumnsAnalyticalAnalyticalAnalyticalAnalyticalGDCGAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativePreparativePreparative33333333355555555555555555101010101010101010101010VS930302-0VS930502-0VS931002-0VS931502-0VS930102-0VS930102-0SVS930505-0VS931005-0VS931505-0VS950302-0VS950502-0VS951002-0VS951502-0VS950505-0VS951005-0VS951505-0VS952505-0VS950105-0VS950105-0SVS951510-0VS952510-0VS950520-0VS951520-0VS952520-0VS950110-0SVS950120-0VS901510-0VS902510-0VS900520-0VS901520-0VS902520-0VS900110-0SVS900120-0VS901030-0VS901530-0VS902530-0VS901550-0VS902550-0VS830302-0VS830502-0VS831002-0VS831502-0VS830102-0VS830102-0SVS830505-0VS831005-0VS831505-0VS850302-0VS850502-0VS851002-0VS851502-0VS850505-0VS851005-0VS851505-0VS852505-0VS850105-0VS850105-0SVS851510-0VS852510-0VS850520-0VS851520-0VS852520-0VS850110-0SVS850120-0VS801510-0VS802510-0VS800520-0VS801520-0VS802520-0VS800110-0SVS800120-0VS801030-0VS801530-0VS802530-0VS801550-0VS802550-0VS650302-0VS650502-0VS651002-0VS651502-0VS650505-0VS651005-0VS651505-0VS652505-0VS650105-0VS650105-0SVS350302-0VS350502-0VS351002-0VS351502-0VS350505-0VS351005-0VS351505-0VS352505-0VS350105-0VS350105-0SVS150302-0VS150502-0VS151002-0VS151502-0VS150505-0VS151005-0VS151505-0VS152505-0VS150105-0VS150105-0SG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersSurface Area: 80m 2 /g, Pore Size: 300ÅTypeParticle(μm)Dimension(mm)VenusilASB C18VenusilASB C8AnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticaGDCG55555555552.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVS950302-TVS950502-TVS951002-TVS951502-TVS950505-TVS951005-TVS951505-TVS952505-TVS950105-TVS950105-TSVS850302-TVS850502-TVS851002-TVS851502-TVS850505-TVS851005-TVS851505-TVS852505-TVS850105-TVS850105-TSG: Guard CartridgeDCG: Direct-connection Guard CartridgeOptimix Family of HPLC ColumnsConventional silica based HPLC stationary phases are usually bonded with mono-type alkyl chain molecules, e.g., C18, C8, etc. Recentstudies have revealed that synergistic effects can be obtained when the bonded surface consists of C18 mixed with a shorter chainsuch as C8, amide or nitro-phenyl, etc. These new products offer different selectivity, coordinating multi-functionality, as well as a spacereffect (C18/C8).It is difficult to produce a uniformly bonded C18 surface on silica due to steric hindrance of the bulky C18 chain. This problem canbe easily overcome when the surface is bonded with mixed chain lengths, C18 and a shorter silane. As a result, there are manyadvantages: improved surface uniformity, better penetration of molecules during partitioning due to less steric hindrance, and differentselectivity compared to a mono-chain type stationary phase.As one of the leaders in separation technologies, Bonna-Agela Technologies has innovatively developed a family of non-ionic, mixedphaseHPLC/SFC columns.024HPLC ColumnsConsider these columns as options for your HPLC method developmentOptimix C18/C8:Improved performance from a mixed-phase C18/C8 (1:1) column compared to mono-chain C18 or C8 columns● Alternative selectivity;● Improved selectivity for stereo isomers, better differentiation of shapes;● Better peak shape due to uniformity of the bonding;● Balanced hydrophilicity and hydrophobicity: extended retention for polar compounds; non-excessive retention for strong hydrophobiccompounds.Characteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYAnalysis of Vancomycin and Its ImpurityH 3 CHONH 2CH 3OOOHHOOHVancomycinH 3 CHONH 2CH 3OOOHHOOHImpurityOOCIOOOOOOOHOHNHHONHHCIH HNOHNHOHNHOHOHONH HH 3 CHNHCH 3OHOHNHHONHHCIH HNOHNHOHNHOHOHONH HH 3 CHNHCH 3HOOCONH 2HOOCONH 2CH 3CH 3HOOHOHHOOHOH025HPLC Columns1.0e+29.5e+19.0e+18.5e+18.0e+17.5e+17.0e+16.5e+112.526.0e+15.5e+15.0e+14.5e+14.0e+13.5e+13.0e+12.5e+12.0e+11.5e+11.0e+111.525.0VancomycinImpurity5.00 10.00 15.00 20.00 25.00 TimeColumn: Optimix C18 /C8, 5μm, 4.6×250mmPart No.: OP952505-OCMobile Phase: A: 0.7% TEA and 3% Acetic Acid in WaterB: Methanol; C: THF ; A:B:C(88:10:2)Flow Rate: 1mL/minTemperature: 25℃Detection: UV 210nmSample: Vancomycin+Impurity1.0e+29.5e+19.0e+18.5e+18.0e+17.5e+1Vancomycin7.0e+16.5e+16.0e+15.5e+15.0e+14.5e+14.0e+13.5e+13.0e+12.5e+12.0e+11.5e+1ImpurityColumn: Brand W C18 Column, 5μm, 4.6×250mmMobile Phase: USP 24A: 0.2% TEA (pH3.2)-Acetonitrile-THF(92:7:1)B: 0.2% TEA (pH3.2)-Acetonitrile-THF(70:29:1)Gradient: USP 241.0e+15.07.5010.0012.5015.0017.5020.00TimeTime(min) 0 12 20 22 23 30B% 0 0 100 100 0 0

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersOptimix C18/AmideA mixed-phase of C18 and propionic amide provides uniqueselectivity which allows extremely polar and non-polar compounds tobe analyzed in a single run under isocratic conditions.Characteristics: Metal Impurity

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYStandard Products!Venusil Family of HPLC Columns -- A Full Line of High Quality ProductsIntroduction of Venusil HPLC ColumnsVenusil HPLC columns are manufactured from the highest purity spherical silica particles on the market. The outstanding propertiesof the HPLC phases are results of subjecting high quality silica to Bonna-Agela’s innovative surface modification and unique bondingprocesses. Our proprietary nanosurface modification generates a very smooth and even surface. This reduces the interaction betweenthe silica surface and polar compounds, resulting in symmetric peak shape even for very basic compounds. Based on this technology,a series of unique columns was developed to meet the tough requirements for the analysis of highly polar compounds. Lot-to-lotreproducibility is ensured by a stable bonding/end-capping process. The uniform, spherical particles have a nominal surface area of380m 2 /g, 200m 2 /g or 80m 2 /g, with a controlled pore size of 100Å, 150Å or 300Å, respectively. All columns are packed using a consistentslurry packing process to achieve uniform and stable beds for maximum column efficiency, lifetime and column-to-column reproducibility.Unprecedented Experience in ChromatographyYou will experience unprecedented performance using Venusil columns for your HPLC separations027HPLC ColumnsBest Peak Symmetry and EfficiencyThe Venusil columns generate symmetric peaks withhigh efficiency over their entire applicable pH range,outperforming competitors.The pH-independent high performance feature ofVenusil columns allows scientists to establish ruggedmethods with flexible choice of pH.Comparison of SpecificationsTightest SpecificationAll our columns have tighter specification (see the table) than otherbrand-name columns. This ensures that all columns have greatperformance and identical selectivity.Spec. on efficiencySpec. on tailing factor(toluene)Spec. on tailing factor-amitriptylineSpec. on selectivity(amitriptyline/acenaphthene)pH rangeSpecific SurfaceCarbon%Characteristics of Venusil ColumnsPerfect peak symmetry, independent of pHand mobile phaseBroad pH range (1.0-10.5)Compatible with 100% aqueous mobile phaseHighest surface coverageA wide variety of chemistriesMinimum buffer concentration neededTightest specificationExcellent column efficiencyGreat column lifetimeWaters Symmetry-C184.6×150mm, 5µmN/AN/A1.8+/- 10%2.0-8.030016%Zorbax XDB-C184.6×150mm, 5µm80000.98-1.25N/A(typically 1.5-2.0)N/A2.0-9.018012%Benefit to the Columns’ usersHighest flexibility for any application, better sensitivity,greater confidence on HPLC resultsFlexibility for method developmentMaximum versatilityTolerance of large volume and mass injectionBroad selection of polarity and maximum versatilityBetter sensitivity for LC/MS, extended column lifetimeBetter reproducibility, greater confidence on the HPLC resultsBetter and faster separationLower cost; greater convenienceVenusil XBP C18 (2)4.6×150mm, 5µm120000.98-1.121.4+/- 7%1.5-9.038018%

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersExpanding the Capabilities with the Venusil Family of C18 ColumnsBonna-Agela Technologies have developed a series of high quality C18 stationary phases to meet a wide range of application needs.These columns contain an ultra pure silica subjected to our patented surface deactivation process. By altering the column chemistry, weare able to tune the surface properties of the silica particles and alter selectivity to meet a variety of application requirement.Many of our columns have very unique properties to address some special needs. Here are some examples:Venusil XBP C18 (2): a phase designed for balanced adsorption to avoid excessive retention of hydrophobic compounds:• Great peak symmetry for all types of compounds• Improved separation of stereo isomers• Extremely narrow specification during manufacturing to offer high column-to-column reproducibility• Non-excessive retention for hydrophobic peaks (less peak broadening of later eluted compounds compared toother columns)Venusil XBP C18:a phase designed for maximum hydrophobicity and high pH tolerance:• The highest carbon loading and the most hydrophobic column on the market.• High pH tolerance• Not suggested for samples containing highly hydrophobic compoundsVenusil XBP C18 (L): a phase designed for larger molecules and highly hydrophobic compounds:• Larger pore size and lower surface area• Accelerated elution for highly hydrophobic compounds• Easier column clean-up for samples containing hydrophobic impurities or samples extracted by non-polar solvents• Better choice for molecules>500 DaltonVenusil AQ C18: a phase designed for polar and semi-polar compounds, to be compatible with 100% water :• Compatible with 100% aqueous to 100% organic mobile phases• Applicable to a variety of analytes: from very polar to non-polar• Operates over a wide pH range: 1.5~9.0• Applicable to a wide range of sample types: plasma, urine, drug formulation and food extraction• Available in a range of column diameters suitable for LC-MS, conventional analytical, and preparative scale028HPLC ColumnsVenusil ASB C18:a phase designed for low pH, low bleed (high sensitivity for LC-MS) and strong separation power for polarcompounds:• Extremely low pH stability: pH limit =0.8 at 70℃• Extremely low bleed offering high sensitivity for LC-MS under acidic conditions• Compatible with 100% aqueous to 100% organic mobile phases• Non-endcapped but with low surface acidity/activity compared to other non-endcapped stationary phases

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil XBP C18 (2) Series ColumnsVenusil XBP C18 (2) column packing material is made with ultra pure silica. The silica surface is processed with Bonna-Agela’s patentedsurface deactivation technology, followed by a unique bonding process that can reduce the carbon content while maintaining a uniformbonding coverage. The Venusil XBP C18 column does not have excessive retention for highly hydrophobic compounds, and it is greatfor the separation of acidic, basic, and neutral compounds. Moreover, this column also has superb resolution power for isomers. Theperfect peak symmetry offered by this RP column makes it a great first-choice for HPLC method development.Universal, Highly Inert C18 Columns• Balanced hydrophobic adsorption which results in a broadersuitability• Stronger separation power for isomers• Great column-to-column and batch-to-batch reproducibility• Perfect symmetry for basic, acidic and neutral compoundsHydrophobicity TestTechnical Parameters• Metal Impuriy 80,000/m (5μm)• TF: 0.98-1.12mAU1201.681100029HPLC Columns8060402000 1 2 3 4 54.711minColumn: Venusil XBP C18 (2), 4.6×150mm, 5μmSample: Uracil, TolueneMobile Phase: MeOH:25mM KH 2 PO 4 /K 2 HPO 4 (pH=6.0)=80:20Detection: UV 254nmFlow Rate: 1mL/minInertness TestColumn: Venusil XBP C18 (2), 4.6×150mm, 5μmSample: AmitriptylineMobile Phase: NaH 2 PO 4 /Na 2 HPO 4 (20mM, pH=6.9):MeOH=29:71Detection: UV 254nmFlow Rate: 1mL/minAmitriptyline is a strong basic antidepressant. It is recognizedas a compound difficult to obtain good peak shape;But the XBP C18(2) column performed outstandingly andgave a great peak symmetry (TF=1.12).

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersExtremely Low Metal EffectsSelectivity of Basic CompoundsVenusil XBP C18(2),4.6×150mmPart.No.:VX951505-2Brand Y C18,4.6×150mmBrand H,4.6×150mmMobile Phase: Water:Methanol=65:35Flow Rate: 1mL/minTemperature: 35℃Detection: UV 230nmSample: 2,3-Dihydroxynaphthalene+ 2,7-DihydroxynaphthaleneMobile Phase: MeOH: Acetic Acid/ sodium acetate aq.(pH=5.0) = 60:40Flow Rate: 1mL/minTemperature: 30℃Detection: UV 254nmSample: p-Methyl benzoic acid, Pyridine, Phenol,Acetophenone, Amino-dimethyl benzene and Toluene030HPLC ColumnsOrdering InformationSurface Area: 380m 2 /g; Pore Size: 100ÅType Particle size(µm)Dimension(mm) Venusil XBP C18 (2)AnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCG55555555552.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVX950302-2VX950502-2VX951002-2VX951502-2VX950505-2VX951005-2VX951505-2VX952505-2VX950105-2VX950105-2SG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil XBP Series ColumnsVenusil XBP C18 columns have the maximum bondingdensity and therefore provide highest hydrophobicity orlowest polarity. This allows for the least interactionbetween the analytes and the silanol groups. Venusil XBPcolumns have extraordinary column stability at high pHs.Pore size: 100Å, 300Å; Specific surface:380m 2 /g,80m 2 /g; Purity>99.999%;1) Inertness: one of most base-friendly columns.2) Efficiency: excellent at any applicable pH.3) Large volume injection: maintain very high efficiency withvery large injection volumes.4) Broad pH range (1.5~10.0 ): low pH, 0.2% TFA; high pH,0.010 M triethylamine.Other Venusil XBP Columns: A complete line of stationaryphases include C8, C4, C1, NH 2 , Phenyl, CN, Silica, SCX, SAX;0.6731.0312.016Quality of Column PackingBrand Z C8Perfect Peak Symmetry and Great Lifetime ofthe Column for Basic Compounds at Mid-pH2.9040.8553.384Initial0310.7381.1902.407Luna C80.7270.6075.8109.77616.755HPLC Columns0.7411.1872.7373.4593.832Venusil XBP C80.7010.6180.8583.8226.04710.257After 10 days17.021Sample: uracil, phenol,chloronitrobenzene,toluene(in methanol/water=50/50)Column: 2.1x50mm, 5μmMobile Phase: methanol:water=65:35Flow Rate: 0.20mL/minTemperature: 30℃Sample: Uracil, Doxepin, Nortriptyline, Amitriptyline,TrimipramineColumn: Venusil XBP-C18, 4.6x150mm, 5μmMobile Phase: 0.01M sodium phosphate:ACN=50:50, pH=7.6Flow Rate: 1.5mL/minTemperature: 45℃Comparison of Venusil C8 PhasesBrand Z-C8Luna C8Venusil XBP C8Plates (Toluene)380525464012Tailing Factor1.121.151.03

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersOrdering InformationSurface Area: 380m 2 /g, Pore Size: 100ÅType Particle(μm) Dimension(mm) Venusil XBP C18 Venusil XBP C8 Venusil XBP C4 Venusil XBP C1Fast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysisAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativeSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativePreparativePreparative3333333335555555555555555555551010101010101010101010102.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250VX930302-0VX930502-0VX931002-0VX931502-0VX930102-0VX930102-0SVX930505-0VX931005-0VX931505-0VX950302-0VX950502-0VX951002-0VX951502-0VX950505-0VX951005-0VX951505-0VX952005-0VX952505-0VX950105-0VX950105-0SVX951510-0VX952510-0VX950520-0VX951520-0VX952520-0VX950110-0SVX950120-0VX951030-0VX951530-0VX952530-0VX901510-0VX902510-0VX900520-0VX901520-0VX902520-0VX900110-0SVX900120-0VX901030-0VX901530-0VX902530-0VX901550-0VX902550-0VX830302-0VX830502-0VX831002-0VX831502-0VX830102-0VX830102-0SVX830505-0VX831005-0VX831505-0VX850302-0VX850502-0VX851002-0VX851502-0VX850505-0VX851005-0VX851505-0VX852005-0VX852505-0VX850105-0VX850105-0SVX851510-0VX852510-0VX850520-0VX851520-0VX852520-0VX850110-0SVX850120-0VX851030-0VX851530-0VX852530-0VX801510-0VX802510-0VX800520-0VX801520-0VX802520-0VX800110-0SVX800120-0VX801030-0VX801530-0VX802530-0VX801550-0VX802550-0VX450302-0VX450502-0VX451002-0VX451502-0VX450505-0VX451005-0VX451505-0VX452005-0VX452505-0VX450105-0VX450105-0SVX150302-0VX150502-0VX151002-0VX151502-0VX150505-0VX151005-0VX151505-0VX152005-0VX152505-0VX150105-0VX150105-0S032HPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYSurface Area: 380m 2 /g, Pore Size: 100ÅTypeAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGParticle(μm)55555555555Dimension(mm)2.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pkVenusilXBP NH 2VN850302-0VN850502-0VN851002-0VN851502-0VN850505-0VN851005-0VN851505-0VN852005-0VN852505-0VN850105-0VN850105-0SVenusilXBP PhenylVX650302-0VX650502-0VX651002-0VX651502-0VX650505-0VX651005-0VX651505-0VX652005-0VX652505-0VX650105-0VX650105-0SVenusilXBP CNVC950302-0VC950502-0VC951002-0VC951502-0VC950505-0VC951005-0VC951505-0VC952005-0VC952505-0VC950105-0VC950105-0SVenusilXBPVSi950302-0VSi950502-0VSi951002-0VSi951502-0VSi950505-0VSi951005-0VSi951505-0VSi952005-0VSi952505-0VSi950105-0VSi950105-0SSurface Area: 80m 2 /g, Pore Size: 300Å033HPLC ColumnsTypeAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGParticle(μm)555555555555555555Dimension(mm)2.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×10VenusilXBP C18VX950302-TVX950502-TVX951002-TVX951502-TVX950505-TVX951005-TVX951505-TVX952005-TVX952505-TVX950105-TVX950105-TSVX951510-TVX952510-TVX950520-TVX951520-TVX952520-TVX950110-TSVX950120-TVenusilXBP C8VX850302-TVX850502-TVX851002-TVX851502-TVX850505-TVX851005-TVX851505-TVX852005-TVX852505-TVX850105-TVX850105-TSVX851510-TVX852510-TVX850520-TVX851520-TVX852520-TVX850110-TSVX850120-TVenusilXBP C4VX450302-TVX450502-TVX451002-TVX451502-TVX450505-TVX451005-TVX451505-TVX452005-TVX452505-TVX450105-TVX450105-TSVenusilXBP C1VX150302-TVX150502-TVX151002-TVX151502-TVX150505-TVX151005-TVX151505-TVX152005-TVX152505-TVX150105-TVX150105-TSSurface Area: 80m 2 /g, Pore Size: 300ÅTypeParticle(μm)Dimension(mm)VenusilXBP NH 2VenusilXBP PhenylVenusilXBP CNVenusilXBPAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCG555555555552.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pkVN850302-TVN850502-TVN851002-TVN851502-TVN850505-TVN851005-TVN851505-TVN852005-TVN852505-TVN850105-TVN850105-TSVX650302-TVX650502-TVX651002-TVX651502-TVX650505-TVX651005-TVX651505-TVX652005-TVX652505-TVX650105-TVX650105-TSVC950302-TVC950502-TVC951002-TVC951502-TVC950505-TVC951005-TVC951505-TVC952005-TVC952505-TVC950105-TVC950105-TSVSi950302-TVSi950502-TVSi951002-TVSi951502-TVSi950505-TVSi951005-TVSi951505-TVSi952005-TVSi952505-TVSi950105-TVSi950105-TSG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil AQ C18 ColumnsThe Venusil AQ C18 column is designed for the separation of polar, medium-polar and non-polar compounds at low to medium pH.This column is more polar than XBP C18, but less polar than ASB C18. With a special surface treatment, Venusil AQ C18 is made to becompatible with 100% aqueous mobile phases, and it can be used to replace Waters Symmetry, Inertsil C18, Zorbax SB, Atlantis dC18,and other AQ columns. This column has a pH range of 1.5-9.0.Pore size:100Å; Specific surface: 380m 2 /g; Purity>99.999%;1) Inertness: one of most base-friendly columns, excellentpeak symmetry for basic compounds.2) Efficiency: The best column for polar compounds at any pH.3) Large volume injection: maintains very high efficiency evenif the injection volume is exceptionally large.4) 100% water compatible: very unique for a universal C18column; much better peak shape, retention, and efficiencythan other brand AQ columns.5) Low pH stability (pH=1.5): better stability than most of thepopular brand columns (including other brand AQ columns)on the market.Compatibility with 100% Aqueous Mobile Phase035HPLC ColumnsSample: RanitidineColumn: 4.6×150mm, 5μmMobile Phase: 100% WaterFlow Rate: 1mL/min; the flow was stopped for 5 minutesduring the testing period for each column.Temperature: 30℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersBalanced Retention for Hydrophilic and Hydrophobic CompoundsVenusil AQ ,4.6×100mm,Part No.: VA951005-0Brand Y AQ ,4.6×100mmMobile Phase: 35% v/v 20.0mM KH 2 PO 4 / K 2 HPO 4at pH=7.0 / 65% Methanol at 23℃Flow Rate: 1mL/minDetection: UV 254nmSample: Uracil Propranolol hydrochlorideButyl, Phthalate Dimethyl PhthalateNaphthalene, Acenaphthene Amitriptyline036Great Peak Shape for All Type of CompoundsmAU400Venusil AQ Column,4.6×250mmPart No.: VA952505-0HPLC Columns30020010000 2 4 6 8 10 12 14 16 18minmAU400Brand Y AQ Column,4.6×250mm300200100002468 10 12 14 16 18minMobile Phase: 1% Acetic Acid (pH=2.57):MeOH=50:50Flow Rate: 1mL/minTemperature: 30℃Detection: 275nmSample: Uracil, Paracetamol, acetophenone and2-Hydroxybenzoic acid

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYOrdering InformationSurface Area: 380m 2 /g, Pore Size: 100ÅTypeParticle(μm)33333333355555555555555555555101010101010101010101010Dimension(mm)2.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250VenusilAQ C18VA930302-0VA930502-0VA931002-0VA931502-0VA930102-0VA930102-0SVA930505-0VA931005-0VA931505-0VA950302-0VA950502-0VA951002-0VA951502-0VA950505-0VA951005-0VA951505-0VA952505-0VA950105-0VA950105-0SVA951510-0VA952510-0VA950520-0VA951520-0VA952520-0VA950110-0SVA950120-0VA951030-0VA951530-0VA952530-0VA901510-0VA902510-0VA900520-0VA901520-0VA902520-0VA900110-0SVA900120-0VA901030-0VA901530-0VA902530-0VA901550-0VA902550-0037HPLC ColumnsFast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysisAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativeSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativePreparativePreparativeG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersVenusil SCX ColumnsSCX Column: made of spherical silica particles of the highest purity (>99.999%), bonded with aromatic sulfonic acid group.They can be used for the separation of basic, water-soluble compounds and bio-molecules.Ordering InformationSurface Area: 200m 2 /g, Pore Size: 150ÅTypeParticle(μm)Dimension(mm)VenusilSCXAnalyticalAnalyticalAnalyticalGDCG555554.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVSc951005-0VSc951505-0VSc952505-0VSc950105-0VSc950105-0SSurface Aera: 80m 2 /g, Pore Size: 300ÅTypeParticle(μm)Dimension(mm)VenusilSCXAnalyticalAnalyticalAnalyticalGDCG555554.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVSc951005-TVSc951505-TVSc952505-TVSc950105-TVSc950105-TS038G: Guard CartridgeDCG: Direct-connection Guard CartridgeHPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYVenusil SAX ColumnsSAX Column: the stationary phase is made with high purity spherical silica particles and bonded with quaternary amine groups.The column can be used for the separation of acidic, water-soluble compounds and bio-molecules.Ordering InformationSurface Area: 380m 2 /g, Pore Size: 100ÅTypeParticle(μm)Dimension(mm)VenusilSAXAnalyticalAnalyticalAnalyticalGDCG555554.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVSa951005-0VSa951505-0VSa952505-0VSa950105-0VSa950105-0SSurface Aera: 80m 2 /g, Pore Size: 300ÅTypeParticle(μm)Dimension(mm)VenusilSAX039AnalyticalAnalyticalAnalyticalGDCG555554.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVSa951005-TVSa951505-TVSa952505-TVSa950105-TVSa950105-TSHPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersPromosil Family of HPLC ColumnsUsing the silica of high-purity and high mechanical strength, the Promosil C18s are made with high-purity monosilane through Bonna-Agela Technologies’ well controlled bonding process. They have high surface bonding coverage and are completely capped. The carboncontent is as much as 18%. They are stable at pH range 1.5-9.0 showing good peak shape for acidic and basic compounds. They haveexcellent tolerance to contamination and long life-time. They are the best choice of high performance-to-cost value.• Double End-cap, Good Inertness• Good Stability and Reproducibility• Excellent Performance-to-cost ValueTechnical Parameters:• High-purity silica, Metal Impurity80,000/m• Tailing Factor: 0.98-1.20Hydrophobicity TestmAU1401201.4961008060402000 1 2 3 4 5Hydrophobicity Test4.348minColumn: Promosil C18, 4.6×150mm, 5μmSample: Uracil, TolueneMobile Phase: MeOH:25mM KH 2 PO 4 /K 2 HPO 4 (pH=6.0)=80:20Detection: UV 254nmFlow Rate: 1mL/min040HPLC ColumnsmAU2001501.6675.440100500-50-1002.9231.8702.4287.3357.8099.87819.97434.155Column: Promosil C18, 4.6×150mm, 5μmSample: AmitriptylineMobile Phase: NaH 2 PO 4 /Na 2 HPO 4 (20mM, pH=6.9):MeOH=32:68Detection: UV 254nmFlow Rate: 1mL/min

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYOrdering InformationSurface Area: 320m 2 /g, Pore Size: 100ÅDimension(mm)2.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×250G 4.6×10, 4/pkDCG 4.6×10, 4/pkParticle(μm)5555555555PromosilC18PM950302-0PM950502-0PM951002-0PM951502-0PM950505-0PM951005-0PM951505-0PM952505-0PM950105-0PM950105-0SPromosilC8PM850302-0PM850502-0PM851002-0PM851502-0PM850505-0PM851005-0PM851505-0PM852505-0PM850105-0PM850105-0SPromosilNH 2PM750302-0PM750502-0PM751002-0PM751502-0PM750505-0PM751005-0PM751505-0PM752505-0PM750105-0PM750105-0SPromosilCNPM550302-0PM550502-0PM551002-0PM551502-0PM550505-0PM551005-0PM551505-0PM552505-0PM550105-0PM550105-0SPromosilSilicaPM050302-0PM050502-0PM051002-0PM051502-0PM050505-0PM051005-0PM051505-0PM052505-0PM050105-0PM050105-0SG: Guard CartridgeDCG: Direct-connection Guard CartridgeOther Manufacturers’ Brands041Bonna-Agela Technologies may also supply a number of major brand name HPLC columns made by other manufacturers, as listedbelow. Please contact us for availability and price information..These brands are: Zorbax, Kromasil, Hypersil, ES-Industry, Shodex, JordiHPLC Columns

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersSelected Solutions for Popular ApplicationsSolutions for Highly Water Soluble CompoundsVenusil AQ C18Venusil ASB C18Unisol AmideIt is known that highly water-soluble compounds often have difficulties in HPLC analysis, such as lack of retention, bad peak shape andinconsistent results. A large scientific effort has been made in developing HPLC stationary phases that can provide solutions to theseproblems. However, no single stationary phase may resolve all these difficulties. Bonna-Agela Technologies has developed a series ofstationary phases along with development schemes to help chemists systematically develop methods for the analysis of highly watersolublecompounds. Some application examples are included as a guide to your column selection.Venusil AQ C18 is a slightly-polar C18 column, and it is our most versatile high aqueous reversed phase column. Venusil AQ C18 has abroad pH suitability (1.5-9.0) and thus can be your first option for most of your HPLC applications. Venusil ASB C18 has pH range of 0.8-7.5. The column stationary phase is non-end capped polar C18 and good for low pH conditions. Unisol Amide (HILIC) has a pH range of2.0-8.0 . Unisol Amide column stationary phase is modified with strong hydrophilic and neutral functionalities, it has hydrophilic interactionwith the compounds. Unisol Amide provides the strongest retention to hydrophilic compounds among these three types of columns.In general, your method development may start with a Venusil AQ C18 column and a mobile phase containing a mixture of methanol oracetonitrile with an aqueous acidic buffer solution (pH=2.0-5.0). This approach may be applicable to the HPLC analysis of more than50% of small molecules (9, another alternative is to use a Unisol Amidecolumn in place of the AQ C18 column and operate at a low to medium pH (2.0-7.0). Figure 3 shows that even with a 100% aqueousmobile phase, validamycin (basic and highly water soluable) still has poor retention on a AQ C18 column. However, a Unisol Amidecolumn operating at pH=2.0 in the hydrophilic interaction mode resulted in a suitable validamycin separation.For neutral compounds, changing pH will have a limited effect on retention. If a suitable method cannot be developed using an AQ C18column, an ASB C18 or Unisol Amide column may be required.Usually during method development, the desired retention is achieved first, then the mobile phase pH and ratio is further adjusted to getthe desired resolution. When the desired retention or resolution cannot be obtained for highly water soluble compounds using a AQ C18or ASB C18 column, a Unisol Amide column will be a good alternative. Unisol Amide columns offer completely different selectivity fromreversed phase columns and offer much higher retentivity .The multiple selection of columns and this comprehensive method development scheme for the separation of highly water-solublecompounds will allow you to develop HPLC methods at ease.

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYFigure 1. HPLC Separation of Organic AcidsFigure 2. HPLC Analysis of Shikimic Acid(3,4,5-Trihydroxy-1-cyclohexene-1-carboxylic Acid)403530270027002522002200mAU20151050Area:21.2573mAU170012007002001.1431.6772.0502.2232 3.7034.7175.5036.0377.2239.22310.03711.39712.0104 13.170170012007002000 1 2 3 4 5 6 7 8 9min0 2 4 6 8 10 12 14minSample: Vitamin C, malonic acid, lactic acid, citric acid.Column: Venusil AQ C18, 4.6x150mm, 5μmMobile Phase: 20mmol phosphate buffer, pH=2.0Detection: UV 210nmFlow Rate: 1.0mL/minTemperature: 30℃Column: Unisol Amide (HILIC), 4.6x250mm, 5μmMobile Phase: ACN/1% formic acid 90-60% in 20minDetection: UV 210nmFlow Rate: 1.0mL/minTemperature: 30℃Figure 3. HPLC Analysis of Validamycin Raw Products043HPLC ColumnsColumn: Brand W, AQ Column, 5μm, 4.6x250mmCH 2OHHOOHOHNHOHOHHOH 2CO OCH 2OHHOOHOHValidamycinColumn: Unisol Amide, 5μm, 4.6x250mmMobile Phase: A:0.1% TFA in WaterB:AcetonitrileGradient: 40% A to 85% A in 30minFlow Rate: 1mL/minTemperature: 25℃Detection: UV 210nmSample: Validamycin

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersAcidic, Basic or NeutralCompounds- Venusil AQ C18 Columns- MeOH/water or ACN/water- pH=2.0-6.0 (TFA, formic acid or other buffer)Good Retention, But NotEnough ResolutionNot Enough Retention- adjust mobile phaseratio or pH- use Venusil ASB C18 orUnisol Amideuse Venusil ASB C18and adjust pH to1.0-2.0; or use UnisolAmideAcidicCompoundsadjust pH to9.0-10.0; oruse UnisolAmideBasicCompoundsuse Unisol Amideand adjustpH to 3.0-7.0NeutralCompoundsOther Stationary Phases from Bonna-Agela Technologies for Hydrophilic Compounds Separation:Unisol C18Venusil XBP Silica (HILIC II)Venusil XBP NH 2 (HILIC III)044HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYUnisol C18HOHOHOCIONHROHO OO N NHHNHHOOHO O HOHOH 3C NHHHOOCNHOOHCIHNTeicoplaninOONHONH 2HOOOHOHOOHOH OHRHOA 2-1A 2-2A 2-3A 2-4A 2-5HO(Z)-4-decenoic acid8-methylnonanoic aicdn-decanoic aicd8-methyldecanioc aicd9-methyldecanioc aicdTeicoplanin045Column: Unisol C18, 4.6×150mmMobile Phase: A:NH 4 AC Buffer (Adjust pH=6.0)B:ACNGradient: 10% B to 60% B in 20min.Flow Rate: 1.0mL/minSample: Teicoplanin+ImpurityTemperature: 35℃HPLC Columns9.08.011.4916332865Nicousamide7.06.0OCOOH5.0NO 2NHOH4.0CHOO3.02.01.00.0CH 3Time5.00 10.00 15.00 20.00 25.00 30.00Column: Unisol C18, 4.6×150mm (UO951505-0)Mobile Phase: Water:ACN:THF (50:50:1, 0.1%H 3 PO 4 )Flow Rate: 1mL/minSample: NicousamideDetection: UV 254nm

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersHILIC Column Family From Bonna-Agela TechnologiesUnisol Amide (Bonna-Agela HILIC )Venusil XBP Silica (Bonna-Agela HILIC II)Venusil XBP NH 2 (Bonna-Agela HILIC III)Comparison:● SelectivityXBP silica is slightly acidic (pH=5.6); while Unisol Amide is slightly basic (pH=7.0-9.0), and XBP NH 2 is more basic (pH=9.2).XBP silica has stronger retention of basic compounds, while Unisol Amide and XBP NH 2 have better retention of acidic compounds.Unisol Amide has a balanced retention power for neutral, acidic and basic compounds, and thus the highest versatility.● Reproducibility and LifetimeUnisol Amide has the best reproducibility and lifetime because of a bonded and close-to-neutral protection layer.Water-soluble Vitamins● Selectivity comparison on 3 columns● Stability comparison of Unisol Amide and XBP silicaVB1VB6VCVB2Unisol Amide Column,5μm, 4.6mm×150mm0 1234 56VB1VB2VB6VCXBP NH 2 Column,5μm, 4.6×150mm046HPLC Columns0 1234 56VB6 VB2VC VB10 1234 56XBP Silica Column,5μm, 4.6×150mmMobile Phase: A:0.1% TFA in Water;B:0.1% TFA in Acetonitrile; A:B=90:10Flow Rate: 1mL/minTemperature: 30℃Detection: UV 280nmSample: VB1+VB6+VC+VB2

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYStability Comparison of Unisol Amide and Venusil XBP SilicaVB6InitialVB6InitialVB1VCVB2VCVB1 VB2After agingAfter agingVB1VB6VCVB6VB2VCVB2VB1Unisol Amide,4.6×150mm,5μmBrabd W Silica,4.6×150mm,5μm047HPLC ColumnsSamples:VB1, VB6, VC and VB2Aging Conditions:MeOH: 20mM NaH 2 PO 4 (pH=7.0)=40:60; 1.0mL/min; Temperature: 40℃HPLC Conditions:Mobile Phase: 0.1%TFA:ACN=90:10;Detection: 280nm; Flow Rate: 1.0mL/min; Temperature: 30℃ ; Injection: 2μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersSolutions for Low pH and High pH ApplicationsBonna-Agela Technologies offer a family of stationary phases for applications at extremely low or extremely high pH. These columnsextend the capability of regular reversed phase HPLC columns to a typical pH range of 2.0-8.0, and provide more options for yourapplications and method development needs.Venusil ASB C18 (pH:0.8-7.5);Venusil ASB C8 (pH:1.0-7.5);Durashell C18 (pH:1.5-12.0)Low pH Stability of ASB C18504030201000 12345678 91401201008060402000 12345678 9High pH Stability of Durashell C18Column: Venusil ASB C18, 4.6×150mm, 5μmSample: NaphtholAging: 40℃ , TFA in 80% methanol (pH=1.0), 400 hoursMobile Phase: TFA in 80% methanol (pH=1.5)Flow Rate: 1.0mL/minInjection: 5μLTemperature: 30℃048HPLC Columns0.700.60Initial0.500.400.300.200.100.00-0.10After 100 h2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00Column: Durashell C18, 4.6×150mm, 5μmFlow Rate: 1.5mL/min, ACN:0.05M Ammonia(pH=9.0)=50:50Sample: Doxepin, nortriptyline, amitriptyline, trimipramineTemperature: 35℃

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYSolutions for LC-MSBonna-Agela Technologies provide columns that can meet the needs of LC-MS applications. The most popular LC-MS columns fromBonna-Agela include:● Unisol C18 (versatile).● Venusil ASB C18 and ASB C8 (extremely low bleed and long lifetime at low pH).● Unisol Amide (for extremely polar compounds).Benefits:● Low bleed and symmetric peak shape→Sensitivity.● High retention for polar compounds→Sensitivity, versatility, and low ion suppression for bioanalytical analysis.● Compatible with 100% water to 100% organic solvents→Simplified method development effort.● Stability→Long column life means a reduced cost for customers.Pseudoephedrine in PlasmaCIHC N NOOH2HCL049HPLC Columns2.8e52.6e52.4e52.2e52.0e51.8e51.6e51.4e51.2e51.0e58.0e46.0e44.0e42.0e40.0PseudoephedrinePseudoephedrineO0.20 .4 0.60 .8 1.01 .2 1.41 .6 1.82 .0 2.22 .4 2.62 .8 3.03 .2Time.min2.124.0e53.5e53.0e52.5e52.0e51.5e51.0e50.5e51.47Lignocaine0.20 .4 0.60 .8 1.01 .2 1.41 .6 1.82 .0 2.22 .4 2.62 .8 3.03 .2Time.minMass System: API Qtrap 3200, Applied BiosystemMass Condition: Positive Ion Mode, MRMHPLC Column: Venusil ASB C18, 2.1×50mm, 5μmSample: Sample prepared by SPE (Cleanert PCX 60mg/3mL)Mobile Phase: A:1% Formic Acid in Water;B:Methanol;Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, thenswitch to A:B (20:80)Flow Rate: 0.5mL/minTemperature: 25℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersAnalysis of 14 Kinds of HormoneMass System: Agilent 1100 Series LC/MSD TrapHPLC Column: Venusil XBP C18, 2.1×150mm, 5μmSample: Estriol, Adosterone, Estrone+Estradial, Testosterone,Dehydro-iso-androsterone Methyl-Testosterone,Epitestosterone, Medroxyprogestrone+Stanolone,Progesetrone, Androsterone, 5-pregen-3beta-20-oneMobile Phase: A:Water; B:MeOHGradient: 40%B to 80%B in 24 min, then hold for 12 min.Flow Rate: 0.8mL/minTemperature: 25℃Oleic Acid in PlasmaOleic acid050Mass System: API Qtrap 3200, Applied BiosystemMass Condition: Negative Ion Mode, MRMHPLC Column: Venusil ASB C18, 2.1×50mm, 5μmSample: Sample prepared by SPE (Cleanert PEP 60mg/3mL)Mobile Phase: A:13mmol/L ammonium acetate aq.,B:AcetonitrileGradient: 5%B to 95%B in 2min, hold for 2min,switch to A:B (95:5) then hold for 2 min.Flow Rate: 0.8mL/minTemperature: 25℃HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYSolutions for Fast AnalysisBonna-Agela Technologies provide two approaches for fast HPLC analyses with shorter run times and higher column efficiencies.● Small particle size or fused-core technology: Venusil AQ C18 (2.5μm), HALO (2.7μm)● Unique retention mechanism : Optimix C18/AmideT=7minMobile Phase: A:NH 4 AC Buffer (Adjust pH=6.0)B:ACNHPLC Column: Halo C18, 4.6mm×50mm, 2.7μmGradient: 5%B to 45%B in10 min.Flow Rate: 1.0mL/minSample: Teicoplanin+ImpurityTemperature: 35℃Conventional C18, 4.6 × 150 mmT=16minMobile Phase: A:NH 4 AC Buffer (Adjust pH=6.0)B:ACNHPLC Column: Conventional C18, 4.6mm×150mm, 5μmGradient: 10%B to 60%B in 20min.Flow Rate: 1.0mL/minSample: Teicoplanin+ImpurityTemperature: 35℃051HPLC ColumnsSolutions for Bio-moleculesBonna-Agela Technologies offers a broad line of HPLC columns for Bio-molecules, including reversed phase, normal phase, ionexchange,HILIC and size exclusion columns. All column packing materials are made of ultra pure silica, bonded with pure silanes toensure the surface inertness.General guide for the column selection:1. Small peptide: C18, C8, C4; 100Å or 150Å2. Large peptide and proteins: C8, C4, 300Å; ion-exchange; HILIC; size exclusion3. Mono- and oligo-saccharides: ion-exchange; NH 2 ; HILIC4. Polysaccharides: ion-exchange; size exclusion5. Oligo-nuclei: ion-exchange; reverse phase6. Nuclei acids: ion-exchange; size exclusionSee the section of “ordering information by type of stationary phase” for ordering or call our technical staff for helps inselecting the columns you need.

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersSolutions for Preparative HPLC(Refer to Purification Section for More Information)Bonna-Agela Technologies offer a full line of preparative HPLC columns to meet a variety of application needs.Our preparative columns feature:1. Great scalability2. Excellent bed stability3. High loading capacity4. Broad solvent compatibility from 100% aqueous to 100% organic solvents (Unisol C18, Unisol Amide and Venusil ASB C18)5. Broad pH range (1.0-12.0) from Durashell6. Unique selectivity from Unisol Amide and Venusil ASB C18Unisol-Amide: a unique phase from Bonna-Agela Technologies that offers a good alternative selectivity to reversed phase columns andan excellent solution for purifying highly polar compounds.Durashell: a high pH tolerance enable the use of high pH mobile phase, which results in great improvement of peak shape andloadability for basic compounds.Solutions for SFCBonna-Agela Technologies offer a broad line of normal phase columns of different selectivity for SFC applications, including:Unisol AmideVenusil XBP NH 2Venusil XBP SilicaVenusil XBP CNVenusil XBP NitrophenylThey are all made of high purity silica materials and are packed using our robust and reproducible process to ensure high efficiency andextended lifetime.052HPLC ColumnsSee the section of “Ordering Information by Type of Stationary Phase” for ordering or call our technical staff for helps.

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYOrdering Information by the Type of Stationary PhasesReversed Phase ColumnsReversed Phase Columns for Small MoleculesAnalytical Columns, Particle Size: 5µm053HPLC ColumnsDimension(mm)2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×250G 4.6×10, 4/pkDCG 4.6×10, 4/pkDimension(mm)2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×250G 4.6×10, 4/pkDCG 4.6×10, 4/pkPromosilC18General C18,100Å, 320m 2 /g;C%=18PM950302-0PM950502-0PM951002-0PM951505-0PM950303-0PM950503-0PM951003-0PM951503-0PM950505-0PM951005-0PM951505-0PM952005-0PM952505-0PM950105-0PM950105-0SVenusilXBP C18 (2)100Å, 380m 2 /g,C%=18VX950302-2VX950502-2VX951002-2VX951502-2VX950303-2VX950503-2VX951003-2VX951503-2VX950505-2VX951005-2VX951505-2VX952005-2VX952505-2VX950105-2VX950105-2SPromosilC8General C8,100Å, 320m 2 /g;C%=10PM850302-0PM850502-0PM851002-0PM851505-0PM850303-0PM850503-0PM851003-0PM851503-0PM850505-0PM851005-0PM851505-0PM852005-0PM852505-0PM850105-0PM850105-0SVenusilXBP C18 (L)150Å, 200m 2 /g;C%=15VX950302-LVX950502-LVX951002-LVX951502-LVX950303-LVX950503-LVX951003-LVX951503-LVX950505-LVX951005-LVX951505-LVX952005-LVX952505-LVX950105-LVX950105-LSPromosilCN100Å, 320m 2 /g,C%=5PM550302-0PM550502-0PM551002-0PM551505-0PM550303-0PM550503-0PM551003-0PM551503-0PM550505-0PM551005-0PM551505-0PM552005-0PM552505-0PM550105-0PM550105-0SInnovalC185μm, 100Å,240m 2 /g;C%=15IX950505-0IX951005-0IX951505-0IX952005-0IX952505-0IX950105-0IX950105-0SVenusilXBP Phenyl100Å, 380m 2 /g, 100Å, 380m 2 /g,C%=12 C%=7VX650302-0VX650502-0VX651002-0VX651502-0VX650303-0VX650503-0VX651003-0VX651503-0VX650505-0VX651005-0VX651505-0VX652005-0VX652505-0VX650105-0VX650105-0SVenusilHLP100Å, 380m 2 /g;C%=15VHL950505-0VHL951005-0VHL951505-0VHL952005-0VHL952505-0VHL950105-0VHL950105-0SVenusilXBP C4VX450302-0VX450502-0VX451002-0VX451502-0VX450303-0VX450503-0VX451003-0VX451503-0VX450505-0VX451005-0VX451505-0VX452005-0VX452505-0VX450105-0VX450105-0SVenusilPFP120Å, 320m 2 /g;VF950505-0VF951005-0VF951505-0VF952005-0VF952505-0VF950105-0VF950105-0SVenusilXBP C1100Å, 380m 2 /g;C%= 3VX150302-0VX150502-0VX151002-0VX151502-0VX150303-0VX150503-0VX151003-0VX151503-0VX150505-0VX151005-0VX151505-0VX152005-0VX152505-0VX150105-0VX150105-0SVenusilPAH200Å, 200m 2 /g;C%=17VP952505-LVP950105-LVP950105-LSVenusilXBP CN100Å, 380m 2 /g,C%=5VC950302-0VC950502-0VC951002-0VC951502-0VC950303-0VC950503-0VC951003-0VC951503-0VC950505-0VC951005-0VC951505-0VC952005-0VC952505-0VC950105-0VC950105-0SG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersDimension(mm)2.1×302.1×502.1×1002.1×150G 2.1×10, 4/pkDCG 2.1×10, 4/pk3.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×250G 4.6×10, 4/pkDCG 4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×250G 10×10G 21.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×250G 10×10G 21.2×1030×10030×15030×25050×15050×250Particle Size(μm)33333333333335555555555555555555555555101010101010101010101010VenusilASB C18150Å, 200m 2 /g;C%=12VS930302-0VS930502-0VS931002-0VS931502-0VS930102-0VS930102-0SVS930303-0VS930503-0VS931003-0VS931503-0VS930505-0VS931005-0VS931505-0VS950302-0VS950502-0VS951002-0VS951502-0VS950303-0VS950503-0VS951003-0VS951503-0VS950505-0VS951005-0VS951505-0VS952005-0VS952505-0VS950105-0VS950105-0SVS951510-0VS952510-0VS950520-0VS951520-0VS952520-0VS950110-0SVS950120-0VS951030-0VS951530-0VS952530-0VS901510-0VS902510-0VS900520-0VS901520-0VS902520-0VS900110-0SVS900120-0VS901030-0VS901530-0VS902530-0VS901550-0VS902550-0VenusilAQ C18100Å, 380m 2 /g,C%=18VA930302-0VA930502-0VA931002-0VA931502-0VA930102-0VA930102-0SVA930303-0VA930503-0VA931003-0VA931503-0VA930505-0VA930505-0VA931505-0VA950302-0VA950502-0VA951002-0VA951502-0VA950303-0VA950503-0VA951003-0VA951503-0VA950505-0VA951005-0VA951505-0VA952005-0VA952505-0VA950105-0VA950105-0SVA951510-0VA952510-0VA950520-0VA951520-0VA952520-0VA950110-0SVA950120-0VA951030-0VA951530-0VA952530-0VA901510-0VA902510-0VA900520-0VA901520-0VA902520-0VA900110-0SVA900120-0VA901030-0VA901530-0VA902530-0VA901550-0VA902550-0UnisolC18100Å, 410m 2 /g,C%=18UO930302-0UO930502-0UO931002-0UO931502-0UO930102-0UO930102-0SUO930303-0UO930503-0UO931003-0UO931503-0UO930505-0UO931005-0UO931505-0UO950302-0UO950502-0UO951002-0UO951502-0UO950303-0UO950503-0UO951003-0UO951503-0UO950505-0UO951005-0UO951505-0UO952005-1UO952505-0UO950105-0UO950105-0SUO951510-0UO952510-0UO950520-0UO951520-0UO952520-0UO950110-0SUO950120-0UO951030-0UO951530-0UO952530-0UO901510-0UO902510-0UO900520-0UO901520-0UO902520-0UO900110-0SUO900120-0UO901030-0UO901530-0UO902530-0UO901550-0UO902550-0DurashellRP150Å, 100m 2 /g,C%=15DS930302-0DS930502-0DS931002-0DS931502-0DS930102-0DS930102-0SDS930303-0DS930503-0DS931003-0DS931503-0DS930505-0DS931005-0DS931505-0DS950302-0DS950502-0DS951002-0DS951502-0DS950303-0DS950503-0DS951003-0DS951503-0DS950505-0DS951005-0DS951505-0DS952005-0DS952505-0DS950105-0DS950105-0SDS951510-0DS952510-0DS950520-0DS951520-0DS952520-0DS950110-0SDS950120-0DS951030-0DS951530-0DS952530-0DS901510-0DS902510-0DS900520-0DS901520-0DS902520-0DS900110-0SDS900120-0DS901030-0DS901530-0DS902530-0DS901550-0DS902550-0DurashellC18100Å, 380m 2 /g,C%=21DC930302-0DC930502-0DC931002-0DC931502-0DC930102-0DC930102-0SDC930303-0DC930503-0DC931003-0DC931503-0DC930502-0DC931002-0DC931505-0DC950302-0DC950502-0DC951002-0DC951502-0DC950303-0DC950503-0DC951003-0DC951503-0DC950505-0DC951005-0DC951505-0DC952005-0DC952505-0DC950105-0DC950105-0SDC951510-0DC952510-0DC950520-0DC951520-0DC952520-0DC950110-0SDC950120-0DC951030-0DC951530-0DC952530-0DC901510-0DC902510-0DC900520-0DC901520-0DC902520-0DC900110-0SDC900120-0DC901030-0DC901530-0DC902530-0DC901550-0DC902550-0VenusilXBP C18100Å, 380m 2 /g,C%=22VX930302-0VX930502-0VX931002-0VX931502-0VX930102-0VX930102-0SVX930303-0VX930503-0VX931003-0VX931503-0VX930505-0VX931005-0VX931505-0VX950302-0VX950502-0VX951002-0VX951502-0VX950303-0VX950503-0VX951003-0VX951503-0VX950505-0VX951005-0VX951505-0VX952005-0VX952505-0VX950105-0VX950105-0SVX951510-0VX952510-0VX950520-0VX951520-0VX952520-0VX950110-0SVX950120-0VX951030-0VX951530-0VX952530-0VX901510-0VX902510-0VX900520-0VX901520-0VX902520-0VX900110-0SVX900120-0VX901030-0VX901530-0VX902530-0VX901550-0VX902550-0VenusilXBP C8100Å, 380m 2 /g;C%= 14VX830302-0VX830502-0VX831002-0VX831502-0VX830102-0VX830102-0SVX830303-0VX830503-0VX831003-0VX831503-0VX830502-0VX831002-0VX831505-0VX850302-0VX850502-0VX851002-0VX851502-0VX850303-0VX850503-0VX851003-0VX851503-0VX850505-0VX851005-0VX851505-0VX852005-0VX852505-0VX850105-0VX850105-0SVX851510-0VX852510-0VX850520-0VX851520-0VX852520-0VX850110-0SVX850120-0VX851030-0VX851530-0VX852530-0VX801510-0VX802510-0VX800520-0VX801520-0VX802520-0VX800110-0SVX800120-0VX801030-0VX801530-0VX802530-0VX801550-0VX802550-0054HPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYDimension(mm)Particle Size(μm)055HPLC Columns2.1×302.1×502.1×1002.1×150G 2.1×10, 4/pkDCG 2.1×10, 4/pk3.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×250G 4.6×10, 4/pkDCG 4.6×10, 4/pk3333333333333555555555555555VenusilASB C8150Å, 200m 2 /g,C%=7VS830302-0VS830502-0VS831002-0VS831502-0VS830102-0VS830102-0SVS830303-0VS830503-0VS831003-0VS831503-0VS830505-0VS831005-0VS831505-0VS850302-0VS850502-0VS851002-0VS851502-0VS850303-0VS850503-0VS851003-0VS851503-0VS850505-0VS851005-0VS851505-0VS852005-0VS852505-0VS850105-0VS850105-0SReversed Phase Columns for Large MoleculesVenusilASB C3150Å, 200m 2 /g,C%=4VS330302-0VS330502-0VS331002-0VS331502-0VS330102-0VS330102-0SVS330303-0VS330503-0VS331003-0VS331503-0VS330505-0VS331005-0VS331505-0VS350302-0VS350502-0VS351002-0VS351502-0VS350303-0VS350503-0VS351003-0VS351503-0VS350505-0VS351005-0VS351505-0VS352005-0VS352505-0VS350105-0VS350105-0SVenusilASB C1150Å, 200m 2 /g,C%=2VS130302-0VS130502-0VS131002-0VS131502-0VS130102-0VS130102-0SVS130303-0VS130503-0VS131003-0VS131503-0VS130505-0VS131005-0VS131505-0VS150302-0VS150502-0VS151002-0VS151502-0VS150303-0VS150503-0VS151003-0VS151503-0VS150505-0VS151005-0VS151505-0VS152005-0VS152505-0VS150105-0VS150105-0SDurashellC8100Å, 380m 2 /g,C%=14DC830302-0DC830502-0DC831002-0DC831502-0DC830102-0DC830102-0SDC830303-0DC830503-0DC831003-0DC831503-0DC830505-0DC831005-0DC831505-0DC850302-0DC850502-0DC851002-0DC851502-0DC850303-0DC850503-0DC851003-0DC851503-0DC850505-0DC851005-0DC851505-0DC852005-1DC852505-0DC850105-0DC850105-0SParticle Size: 5µm; Surface Area: 80m 2 /g; Pore Size: 300Å;Column Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CNAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCG2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pkG: Guard CartridgeDCG: Direct-connection Guard CartridgeVX650302-TVX650502-TVX651002-TVX651502-TVX650303-TVX650503-TVX651003-TVX651503-TVX650505-TVX651005-TVX651505-TVX652005-TVX652505-TVX650105-TVX650105-TSVX450302-TVX450502-TVX451002-TVX451502-TVX450303-TVX450503-TVX451003-TVX451503-TVX450505-TVX451005-TVX451505-TVX452005-TVX452505-TVX450105-TVX450105-TSVX150302-TVX150502-TVX151002-TVX151502-TVX150303-TVX150503-TVX151003-TVX151503-TVX150505-TVX151005-TVX151505-TVX152005-TVX152505-TVX150105-TVX150105-TSVC950302-TVC950502-TVC951002-TVC951502-TVC950303-TVC950503-TVC951003-TVC951503-TVC950505-TVC951005-TVC951505-TVC952005-TVC952505-TVC950105-TVC950105-TS

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersColumn Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CNPreparativePreparativePreparativePreparativePreparativeGG10×15010×25021.2×5021.2×15021.2×25010×1021.2×10VX651510-TVX652510-TVX650520-TVX651520-TVX652520-TVX650110-TSVX650120-TVX451510-TVX452510-TVX450520-TVX451520-TVX452520-TVX450110-TSVX450120-TVX151510-TVX152510-TVX150520-TVX151520-TVX152520-TVX150110-TSVX150120-TVC951510-TVC952510-TVC950520-TVC951520-TVC952520-TVC950110-TSVC950120-TParticle Size: 5μm; Surface Area: 80m 2 /g; Pore Size: 300Å;Column Type Dimension (mm) Venusil ASB C18AnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGPreparativePreparativePreparativePreparativePreparativeGG2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×10G: Guard CartridgeDCG: Direct-connection Guard Cartridgeextreme low pHtolerance (0.8-7.5),low bleedVS950302-TVS950502-TVS951002-TVS951502-TVS950303-TVS950503-TVS951003-TVS951503-TVS950505-TVS951005-TVS951505-TVS952005-TVS952505-TVS950105-TVS950105-TSVS951510-TVS952510-TVS950520-TVS951520-TVS952520-TVS950110-TSVS950120-TVenusil ASB C8 Venusil XBP C18 Venusil XBP C8C8 for low pHand low bleedVS850302-TVS850502-TVS851002-TVS851502-TVS850303-TVS850503-TVS851003-TVS851503-TVS850505-TVS851005-TVS851505-TVS852005-TVS852505-TVS850105-TVS850105-TSVS851510-TVS852510-TVS850520-TVS851520-TVS852520-TVS850110-TSVS850120-ThighesthydrophobicityVX950302-TVX950502-TVX951002-TVX951502-TVX950303-TVX950503-TVX951003-TVX951503-TVX950505-TVX951005-TVX951505-TVX952005-TVX952505-TVX950105-TVX950105-TSVX951510-TVX952510-TVX950520-TVX951520-TVX952520-TVX950110-TSVX950120-TGeneral RPVX850302-TVX850502-TVX851002-TVX851502-TVX850303-TVX850503-TVX851003-TVX851503-TVX850505-TVX851005-TVX851505-TVX852005-TVX852505-TVX850105-TVX850105-TVX851510-TVX852510-TVX850520-TVX851520-TVX852520-TVX850110-TSVX850120-T056HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYNormal Phase ColumnsNormal Phase Columns for Small MoleculesParticle Size: 5μmTypeParticleSize(μm)Dimension(mm)Unisol Amide(HILIC)Venusil XBPSilica(HILIC II)Venusil XBPNH 2(HILIC III)VenusilXBP CNDurashellNH 2VenusilXBP Diol100Å,410m 2 /g,100Å,380m 2 /g,100Å,380m 2 /g;100Å,380m 2 /g,100Å,380m 2 /g,100Å,380m 2 /g;057HPLC ColumnsAnalytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5Analytical 5G5DCG5Semi-preparative 5Semi-preparative 5Preparative 5Preparative 5Preparative 5G5G5Preparative 5Preparative 5Preparative 5Semi-preparative 10Semi-preparative 10Preparative 10Preparative 10Preparative 10G10G10Preparative 10Preparative 10Preparative 10Preparative 10Preparative 102.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25010×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×25050×15050×250VH950302-0VH950502-0VH951002-0VH951502-0VH950303-0VH950503-0VH951003-0VH951503-0VH950505-0VH951005-0VH951505-0VH952005-0VH952505-0VH950105-0VH950105-0SVH951510-0VH952510-0VH950520-0VH951520-0VH952520-0VH950110-0SVH950120-0VH951030-0VH951530-0VH952530-0VH901510-0VH902510-0VH900520-0VH901520-0VH902520-0VH900110-0SVH900120-0VH901030-0VH901530-0VH902530-0VH901550-0VH902550-0VSi950302-0VSi950502-0VSi951002-0VSi951502-0VSi950303-0VSi950503-0VSi951003-0VSi951503-0VSi950505-0VSi951005-0VSi951505-0VSi952005-0VSi952505-0VSi950105-0VSi950105-0SVSi951510-0VSi952510-0VSi950520-0VSi951520-0VSi952520-0VSi950110-0SVSi950120-0VSi951030-0VSi951530-0VSi952530-0VSi901510-0VSi902510-0VSi900520-0VSi901520-0VSi902520-0VSi900110-0SVSi900120-0VSi901030-0VSi901530-0VSi902530-0VSi901550-0VSi902550-0VN850302-0VN850502-0VN851002-0VN851502-0VN850303-0VN850503-0VN851003-0VN851503-0VN850505-0VN851005-0VN851505-0VN852005-0VN852505-0VN850105-0VN850105-0SVN851510-0VN852510-0VN850520-0VN851520-0VN852520-0VN850110-0SVN850120-0VN851030-0VN851530-0VN852530-0VN801510-0VN802510-0VN800520-0VN801520-0VN802520-0VN800110-0SVN800120-0VN801030-0VN801530-0VN802530-0VN801550-0VN802550-0VC950302-0VC950502-0VC951002-0VC951502-0VC950303-0VC950503-0VC951003-0VC951503-0VC950505-0VC951005-0VC951505-0VC952005-0VC952505-0VC950105-0VC950105-0SVC951510-0VC952510-0VC950520-0VC951520-0VC952520-0VC950110-0SVC950120-0VC951030-0VC951530-0VC952530-0VC901510-0VC902510-0VC900520-0VC901520-0VC902520-0VC900110-0SVC900120-0VC901030-0VC901530-0VC902530-0VC901550-0VC902550-0DN850302-0 VD950302-0DN850502-0 VD950502-0DN851002-0 VD951002-0DN851502-0 VD951502-0DN850303-0 VD950303-0DN850503-0 VD950503-0DN851003-0 VD951003-0DN851503-0 VD951503-0DN850505-0 VD950505-0DN851005-0 VD951005-0DN851505-0 VD951505-0DN852005-0 VD952005-0DN852505-0 VD952505-0DN850105-0 VD950105-0DN850105-0S VD950105-0SDN851510-0DN852510-0DN850520-0DN851520-0DN852520-0DN850110-0SDN850120-0DN851030-0DN851530-0DN852530-0G: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersHigh Purity; Surface Aera: 320m 2 /g; Pore Size: 100Å;Type Particle Size (µm) Dimension (mm) Promosil Silica Promosil CN Promosil NH 2AnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparative5555555555555555555555555G: Guard CartridgeDCG: Direct-connection Guard Cartridge2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×250PM050302-0PM050502-0PM051002-0PM051502-0PM050303-0PM050503-0PM051003-0PM051503-0PM050505-0PM051005-0PM051505-0PM052005-0PM052505-0PM050105-0PM050105-0SPM051510-0PM052510-0PM050520-0PM051520-0PM052520-0PM050110-0SPM050120-0PM051030-0PM051530-0PM052530-0PM550302-0PM550502-0PM551002-0PM551502-0PM550303-0PM550503-0PM551003-0PM551503-0PM550505-0PM551005-0PM551505-0PM552005-0PM552505-0PM550105-0PM550105-0SPM750302-0PM750502-0PM751002-0PM751505-0PM750303-0PM750503-0PM751003-0PM751503-0PM750505-0PM751005-0PM751505-0PM752005-0PM752505-0PM750105-0PM750105-0S058HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYNormal Phase Columns for Large MoleculesUltra Pure Silica; Surface Area: 80m 2 /g; Pore Size: 300Å059HPLC HPLC Columns ColumnsTypeAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGGSemi-preparativeSemi-preparativePreparativePreparativePreparativeGGPreparativePreparativePreparativeParticleSize(μm)5555555555555555555555555Dimension(mm)2.1×302.1×502.1×1002.1×1503.0x303.0x503.0x1003.0x1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk10×15010×25021.2×5021.2×15021.2×25010×1021.2×1030×10030×15030×250VenusilXBP NH 2VN850302-TVN850502-TVN851002-TVN851502-TVN850303-TVN850503-TVN851003-TVN851503-TVN850505-TVN851005-TVN851505-TVN852005-TVN852505-TVN850105-TVN850105-TSVN851510-TVN852510-TVN850520-TVN851520-TVN852520-TVN850110-TSVN850120-TVN851030-TVN851530-TVN852530-TVenusilXBP DiolVD950302-TVD950502-TVD951002-TVD951502-TVD950303-TVD950503-TVD951003-TVD951503-TVD950505-TVD951005-TVD951505-TVD952005-TVD952505-TVD950105-TVD950105-TSVenusilXBP CNVC950302-TVC950502-TVC951002-TVC951502-TVC950303-TVC950503-TVC951003-TVC951503-TVC950505-TVC951005-TVC951505-TVC952005-TVC952505-TVC950105-TVC950105-TSVC951510-TVC952510-TVC950520-TVC951520-TVC952520-TVC950110-TSVC950120-TVC951030-TVC951530-TVC952530-TUnisol Amide(HILIC)VH950302-TVH950502-TVH951002-TVH951502-TVH950303-TVH950503-TVH951003-TVH951503-TVH950505-TVH951005-TVH951505-TVH952005-TVH952505-TVH950105-TVH950105-TSVenusilXBP SilicaVSi950302-TVSi950502-TVSi951002-TVSi951502-TVSi950303-TVSi950503-TVSi951003-TVSi951503-TVSi950505-TVSi951005-TVSi951505-TVSi952005-TVSi952505-TVSi950105-TVSi950105-TSVSi951510-TVSi952510-TVSi950520-TVSi951520-TVSi952520-TVSi950110-TSVSi950120-TVSi951030-TVSi951530-TVSi952530-TIon-exchange ColumnsTypeAnalyticalAnalyticalAnalyticalGDCGParticle Size(μm)55555Dimension(mm)4.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVenusilSAXVenusilSCX100Å, 380m 2 /g 150Å, 200m 2 /gVSa951005-0VSc951005-0VSa951505-0VSc951505-0VSa952505-0VSc952505-0VSa950105-0VSc950105-0VSa950105-0SVSc950105-0SG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersSurface Aera: 80m 2 /g, Pore Size: 300ÅTypeParticle Size(μm)Dimension(mm)VenusilSAXVenusilSCXAnalyticalAnalyticalAnalyticalGDCG555554.6×1004.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pkVSa951005-TVSa951505-TVSa952505-TVSa950105-TVSa950105-TSVSc951005-TVSc951505-TVSc952505-TVSc950105-TVSc950105-TSMixed Phase ColumnsSurface Area: 380m 2 /g, Pore Size: 100ÅType Dimension (mm) Particle size(µm) Optimix C18/C8(1:1) Optimix C18/amide(1:1) Optimix C18/phenyl(1:1)AnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGFast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysis2.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk2.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×15055555555555333333333OP950302-OCOP950502-OCOP951002-OCOP951502-OCOP950505-OCOP951005-OCOP951505-OCOP952005-OCOP952505-OCOP950105-OCOP950105-OCSOP950302-AMOP950502-AMOP951002-AMOP951502-AMOP950505-AMOP951005-AMOP951505-AMOP952005-AMOP952505-AMOP950105-AMOP950105-AMSOP930302-AMOP930502-AMOP931002-AMOP931502-AMOP930102-AMOP930102-AMSOP930505-AMOP931005-AMOP931505-AMOP950302-PHOP950502-PHOP951002-PHOP951502-PHOP950505-PHOP951005-PHOP951505-PHOP952005-PHOP952505-PHOP950105-PHOP950105-PHS060HPLC ColumnsG: Guard CartridgeDCG: Direct-connection Guard Cartridge

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYTypeFast analysisFast analysisFast analysisFast analysisGDCGFast analysisFast analysisFast analysisAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalAnalyticalGDCGDimension (mm) Particle size(µm) Optimix C18/SCX(1:1) Optimix C18/SAX(1:1) Optimix C18/Nitrophenyl(1:1)2.1×302.1×502.1×1002.1×1502.1×10, 4/pk2.1×10, 4/pk4.6×504.6×1004.6×1502.1×302.1×502.1×1002.1×1504.6×504.6×1004.6×1504.6×2004.6×2504.6×10, 4/pk4.6×10, 4/pk33333333355555555555OP930302-SCOP930502-SCOP931002-SCOP931502-SCOP930102-SCOP930102-SCSOP930505-SCOP931005-SCOP931505-SCOP950302-SCOP950502-SCOP951002-SCOP951502-SCOP950505-SCOP951005-SCOP951505-SCOP952005-SCOP952505-SCOP950105-SCOP950105-SCSOP950302-SAOP950502-SAOP951002-SAOP951502-SAOP950505-SAOP951005-SAOP951505-SAOP952005-SAOP952505-SAOP950105-SAOP950105-SASOP950302-NPOP950502-NPOP951002-NPOP951502-NPOP950505-NPOP951005-NPOP951505-NPOP952005-NPOP952505-NPOP950105-NPOP950105-NPSSize Exclusion Columns (Silica Based)061HPLC ColumnsTypeAnalyticalAnalyticalAnalyticalAnalyticalSemi-prep.Semi-prep.Semi-prep.Semi-prep.Dimension(mm)4.6x1504.6x2504.6x1504.6x25010x15010x25010x15010x250Particle Size(μm)551010551010GF GF 300 GF amide GFamide-300ultra pure, high porosity;bonded 120Å,GF151505-0GF152505-0GF101505-0GF102505-0GF151510-0GF152510-0GF101510-0GF102510-0ultra pure, high porosity;bonded 300Å,GF351505-0GF352505-0GF301505-0GF302505-0GF351510-0GF352510-0GF301510-0GF302510-0ultra pure, high porosity;bonded with amide 120Å,GF151505-AGF152505-AGF101505-AGF102505-AGF151510-AGF152510-AGF101510-AGF102510-Aultra pure, high porosity;bonded with amide 300Å,GF351505-AGF352505-AGF301505-AGF302505-AGF351510-AGF352510-AGF301510-AGF302510-AChiral ColumnsParticle size: 5μm; Pore Size: 1000ÅTypeDimension(mm)Chiral AD-HChiral OD-HChiral OJ-HAnalyticalAnalyticalGDCGPreparativeGG4.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pk21.2×25010×1021.2×10AD951505-0AD952505-0AD950105-0AD950105-0SAD952520-0AD950110-0SAD950120-0OD951505-0OD952505-0OD950105-0OD950105-0SOD952520-0OD950110-0SOD950120-0OJ951505-0OJ952505-0OJ950105-0OJ950105-0SOJ952520-0OJ950110-0SOJ950120-0G: Guard CartridgeDCG: Direct-connection Guard Cartridge

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersParticle size: 5μm; Pore Size: 300ÅTypeAnalyticalAnalyticalGDCGPreparativeGGDimension(mm)4.6×1504.6×2504.6×10, 4/pk4.6×10, 4/pk21.2×25010×1021.2×10Chiral Amide-1AM951505-1AM952505-1AM950105-1AM950105-1SAM952520-1AM950110-1SAM950120-1Polymer Phase ColumnsRPC-Analytical Prepacked ColumnsMatrixAKF-RP-M-154607AKF-RP-M-154610AKF-RP-M-154620AKF-RP-H-254605AKF-RP-H-254607AKF-RP-H-254610AKF-RP-H-254620RPC-Preparative Prepacked ColumnsMatrixAKF-RP-M-052007AKF-RP-M-102007AKF-RP-M-251007AKF-RP-M-252007AKF-RP-M-151010AKF-RP-M-152010AKF-RP-H-151010AKF-RP-H-152010AKF-RP-M-251010AKF-RP-M-252010AKF-RP-H-251010AKF-RP-H-252010AKF-RP-H-152020AKF-RP-H-252020AKF-RP-H-105020AKF-RP-H-255020AKF-RP-H-2510020G: Guard CartridgeDCG: Direct-connection Guard CartridgeDimension(mm)4.6×1504.6×1504.6×1504.6×2504.6×2504.6×2504.6×250Dimension(mm)20×5020×10010×25020×25010×15020×15010×15020×15010×25020×25010×25020×25020×15020×25050×10050×250100×250Particle Size(μm)71020571020Particle Size(μm)557710101010101010102020202020062HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYHydrophobic Interaction Chromatography ColumnsMatrixDimension(mm)Particle Size(μm)063HPLC ColumnsAKF-PHEMM-154607AKF-PHEMM-154610AKF-PHEMM-154620AKF-PHEMH-254607AKF-PHEMH-254610AKF-PHEMH-254620AKF-PHEMH-036410AKF-C4-MM-154607AKF-C4-MM-154610AKF-C4-MM-154620AKF-C4-MH-254607AKF-C4-MH-254610AKF-C4-MH-254620AKF-C4-MH-036410AKF-C8-MM-154607AKF-C8-MM-154610AKF-C8-MM-154620AKF-C8-MH-254607AKF-C8-MH-254610AKF-C8-MH-254620AKF-C8-MH-036410AKF-ETHMM-154607AKF-ETHMM-154610AKF-ETHMM-154620AKF-ETHMH-254607AKF-ETHMH-254610AKF-ETHMH-254620AKF-ETHMH-0364104.6×1504.6×1504.6×1504.6×2504.6×2504.6×2506.4×304.6×1504.6×1504.6×1504.6×2504.6×2504.6×2506.4×304.6×1504.6×1504.6×1504.6×2504.6×2504.6×2506.4×304.6×1504.6×1504.6×1504.6×2504.6×2504.6×2506.4×30710207102010710207102010710207102010710207102010Ion Exchange Chromatography Columns (Strong Cation)MatrixAKF-CISLM-050207AKF-CISLM-150207AKF-CISLM-154607AKF-CISLM-154610AKF-CISLM-154620AKF-CISLH-154607AKF-CISLH-154610AKF-CISLH-154620AKF-CISLM-254610AKF-CISLH-254610AKF-CISLH-251010AKF-CISLH-101010Dimension(mm)2.0×502.0×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×2504.6×25010×25010×100Particle Size(μm)55710207102010101010

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersIon Exchange Chromatography Columns (Weak Cation)MatrixAKF-CLV-050207AKF-CLV-150207AKF-CLV-154607AKF-CLV-154610AKF-CLV-154620AKF-CHV-154607AKF-CHV-154610AKF-CHV-154620AKF-CLV-254610AKF-CHV-254610AKF-CHV-251010AKF-CHV-101010Dimension(mm)2.0×502.0×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×2504.6×25010×25010×100Particle Size(μm)55710207102010101010Ion Exchange Chromatography Columns (Strong Anion)MatrixAKF-QLV-050207AKF-QLV-150207AKF-QLV-154607AKF-QLV-154610AKF-QLV-154620AKF-QHV-154607AKF-QHV-154610AKF-QHV-154620AKF-QLV-254610AKF-QHV-254610AKF-QHV-251010AKF-QHV-101010Dimension(mm)2.0×502.0×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×2504.6×25010×25010×100Particle Size(μm)55710207102010101010064HPLC ColumnsIon Exchange Chromatography Columns (Weak Anion)MatrixAKF-DEAL-050207AKF-DEAL-150207AKF-DEAL-154607AKF-DEAL-154610AKF-DEAL-154620AKF-DEAH-154607AKF-DEAH-154610AKF-DEAH-154620AKF-DEAL-254610AKF-DEAH-254610AKF-DEAH-251010AKF-DEAH-101010Dimension(mm)2.0×502.0×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×1504.6×2504.6×25010×25010×100Particle Size(μm)55710207102010101010

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYGuard Cartridge HolderTypeAnalyticalAnalyticalDimension(mm)4.62.1P/NCH-100CH-50TypePreparativeDimension(mm)10P/NSH-150TypePreparativeDimension(mm)20P/NCH-200Direct-connection Guard Cartridge HolderTypeAnalyticalAnalyticalDimension(mm)4.62.1P/NSH-100SH-100065HPLC Columns

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersHPLC Accessories and SuppliesHPLC Fitting and TubingCat.No.MaterialTubing Size/Nut ThreadsAF-120PM1960PM1945RBX-0400BX-0300BX-0200BX-0100BX-0070FF-1600FF2400PEEKPEEKPEEKStainless SteelStainless SteelStainless SteelStainless SteelStainless SteelTeflon pipeTeflon pipe1/16”, 10-321/16''OD, 0.010''(0.25mm)ID, 1m1/16''OD, 0.005''(125um)ID, 1m1/16"OD, 0.04"ID, 20cm1/16"OD, 0.03"ID, 20cm1/16"OD, 0.02"ID, 20cm1/16"OD, 0.01"ID, 20cm1/16"OD, 0.007"ID, 20cmID 1.6mmID 2.4mmCat.No.: AF-120Automatic Column Selector● Connect up to 6 HPLC columns● Controllable by both panel keys and computer● Easy key operation for setting method and sequence● Compact design and easy access to columns● Compatible with any HPLCHPLC Column HeaterCat.No.: LC-08066HPLC ColumnsTechnical specificationCat.No.Dimensions (H×W×D)Operating TemperatureTemperature AccuracyCompatible Column Quantity and LengthPowerWeightCC-100510mm×110mm×65mmAbove 5℃ ~60℃±0.1℃2×300mm220V/50Hz/1A4KgCat.No.: CC-100UV LampsBonna-Agela Technologies offers UV lamps of the same or better quality as a low costreplacement for many of those manufactured by original HPLC manufacturers, such asShimadzu.

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYShimadzuOriginal Instrument Part NumberSPD-10A,2000hLC2010,2000hSPD-20A,2000hUV AABonna-Agela Lamps Part NumberSHL001SHL002SHL003SUV001Pipettes and AccessoriesMechanical Pipette (Fixed Volume)Cat No.Volume (μL)Cat No.Volume (μL)A722001A722004A722010A722015A722020A722025510202550100A722030A722035A722040A722045A722050A722055200250500100020005000067HPLC ColumnsMechanical Pipette (Adjustable Volume)Single Channel PipetteCat No.A720005A720000A720020A720080A720050Volume (μL)0.1-2.50.5-105-502-2010-100Increment (μL)0.050.10.50.51Cat No.A720070A720030A720060A720040A720110Volume (μL)20-20050-200100-1000200-10001000-5000Increment (μL)1155508-Channel Pipette12-Channel PipetteCat No.Volume (μL)Increment (μL)Cat No.Volume (μL)Increment (μL)A720210A720220A7202400.5-105-5050-3000.10.55A720310A720320A7203400.5-105-5050-3000.10.55Pipette TipsCat No.QtyCat No.QtyAD781348AD781349AD780006AD780007FT10μLFT10μLFT300μLFT300μLAD780019AD780020AD780046FT1000μLFT1000μLFT1200μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersHPLC Column Selection GuideColumn Selection ParametersStationary Phases• Reversed Phase [C18 (Unisol, XBP, AQ, ASB), C8 (XBP,ASB), C4, Phenyl ]: most HPLC analytical andpreparative separations; use shorter chain if the retentionis too high on C18 columns; use shorter chain (C8, C4)for proteins and larger peptides.• Normal Phase [Silica, Amino, SAX, SCX]: for those notapplicable on reversed phase; polysaccharides(amino),ion-exchange chromatography, some preparative needs.• Bi-mode [Cyano, Venusil HILIC]: can be used in bothreversed and normal phase modes, alternative selectivityto hydrocarbon-based reversed phases, inert and betterreproducibility than silica columns.Particle Size• 3μm: fast analysis, high throughput analyticalapplications, micro/nano HPLC• 5μm: analytical and semi-preparative separation• 10μm: preparative separationParticle Size• Narrow pore (100-150Å): MW10mm for preparative).• Sample volume: small volume, small diameter; largevolume, large diameter and length.• Sample complexity: simple separation, short column;complex separation, long column.Instrument and Application• Traditional HPLC analysis: 3μm, 5μm; 4.6x100mm,4.6x150mm, 4.6x250mm• High throughput analysis: 3μm, 5μm; 2.1x30mm,2.1x50mm, 4.6x50mm.• LC-MS application: 3μm, 5μm; 1.0x30mm,1.0x50mm, 2.1x30mm, 2.1x50mm, 2.1x100mm.• Micro HPLC: 3μm, 5μm; 0.5x30mm, 0.5x50mm,0.5x100mm.• Preparative HPLC: 5μm, 10μm; 21.2x50mm,21.2x150mm, 21.2x250mm, 30x150mm,30x250mm, 50x250mm.HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYCross-reference for Bonna-Agela Venusil Series Columns069HPLC ColumnsAgilentSB C18SB C8XDB C18XDB C8SB AqWatersSunfire C18Sunfire C8Atlantis C18 (AQ)Atlantis HILICPhenomenexLuna C18Luna C8Luna C18 (2)SupelcoDiscovery C18Discovery C8Asentics C18Asentics C8GL ScienceInsertsil ODS-3E: Similar selectivity and superior performanceR: A better replacementXBP C18 XBP C18 (2) XBP C8 XBP(L) C18 XBP(L) C8 ASB C18 ASB C8 AQ C18 HILICREEREREEREComparison of Particle Characteristics of Typical HPLC Stationary PhasesRERRRRERStationary Phase Particle(μm) Pore(A) Surface(m 2 /g) C% End Capping High Purity SilicaVenusil XBP C18 (2)Venusil XBP C18Venusil AQ C18Venusil ASB C18Venusil XBP C18(L)ZORBAX SB C18ZORBAX XDB C18Waters SunFire C18Waters Symmetry C18Waters XTerra MS C18Hypersil BDS C18Hypersil ODSInertsil ODSInertsil ODS3Kromasil C18Supelco Discovery C18Luna C18(2)555555555555555551001001001501508080100100125130120100100100180100380380380200200180180340335—17017035045034020040018221812151012161915.511101415191217.5doubledoubleyesnodoublenoyesyesyesyesyesyesyesyesyesyesyesyesyesyesyseyesyesyesyesyes—nononoyesspecialyesyes

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersUSP Column Selection GuideL01Octadecyl silane (ODS or C18) chemically bondedto porous silica or ceramic particles,1.5~10μm indiameter.L08An essentially monomolecular layer of aminopropylsilane(NH 2 ) chemically bonded to totally poroussilica gel support, 3~10μm in diameter.L02BrandUnisol C18Venusil ASB C18Venusil XBP C18Venusil XBP C18 (2)Venusil XBP C18 (L)Durashell C18Promosil C18Halo C18Particle(μm)3, 5, 103, 5, 103, 5, 10553, 5, 1052.7FigurespherespherespherespherespherespheresphereOctadecyl silane (ODS or C18) chemically bonded tosilica gel of a controlled surface porosity bonded to asolid spherical core, 30~50μm in diameter.L09L10BrandVenusil XBP NH 2Promosil NH 23~10μm irregular, totally porous silica gel having achemically bonded strongly acidic cation exchangercoating (SCX).BrandVenusil SCXParticle(μm)3, 55Particle(μm)5FigurespheresphereFiguresphereNitrile groups (CN) chemically bonded to porous silicaparticles, 3~10μm in diameter.BrandC18 bulk mediaParticle(μm)50FigureIrregularBrandVenusil XBP CNParticle(μm)3, 5FiguresphereL03Porous silica particles, 1.5~10μm in diameter.L11Phenyl groups chemically bonded to porous silicaparticles, 1.5~10μm in diameter.070L04L05L06L07BrandVenusil XBP SilicaVenusil XBP Silica(L)Promosil SilicaBrandspherespheresphereSilica gel of a controlled surface porosity bonded to asolid spherical core, 30~50μm in diameter.Bulk media(Silica)IrregularAlumina of controlled surface porosity bonded to asolid spherical core, 30~50μm in diameter.Strong cation exchanger packing-sulfonated fluorocarbonpolymer coated on a solid spherical core,30~50μm in diameter.Octyl silane (C8) chemically bonded to porous silicaparticles,1.5~10μm in diameter.BrandVenusil ASB C8Venusil XBP C8Venusil XBP C8(L)Halo C8Particle(μm)3, 5, 1055Particle(μm)30~50Particle(μm)3, 5, 103, 5, 1052.7FigureFigureFigurespherespherespheresphereL12L13L14L15L16BrandVenusil XBP PhenylBrandsphereA strong anion exchanger packing madeby chemicallybonding a quaternary amine to a solid silica sphericalcore, 30~50μm in diameter.Trimethylsilane (C1) chemically bonded to poroussilica particles, 3~10μm in diameter.Venusil XBP C1BrandsphereSilica gel, 5~10μm in diameter having a chemicallybonded, strongly basic quaternary ammonium anionexchanger (SAX) coating.Venusil SAXParticle(μm)3, 5Particle(μm)3, 5Particle(μm)FigureFigureFiguresphereHexylsilane (C6) chemically bonded to a totally poroussilica particle, 3~10μm in diameter.Dimethylsilane (C2) chemically bonded to a totallyporous silica particles, 5~10μm in diameter.5HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYL17Strong cation exchange resin consisting of sulfonated,cross-linked styrene divinylbenzene copolymer in thehydrogen form, 7~11μm in diameter.L27Porous silica particles, 30~50μm in diameter.BrandAKF CISLHParticle(μm)7FiguresphereBrandBulk media(Silica)Particle(μm)50FigureIrregularL18L19BrandAmino (NH 2 ) and Cyano (CN) groups chemicallybonded to porous silica particles, 3~10μm in diameter.Strong cation exchange resin consisting of sulfonated,cross-linked styrene divinyl benzene copolymer in thecalcium form, about 9μm in diameter.AKF CISLMParticle(μm)7FiguresphereL28L29A multifunctional support which consists of a highpurity, 100Å, spherical silica ssubstrate that has beenbonded with anionic functionality in addition to aconventional reversed-phase C8 functionality.Gamma alumina, reversed-phase, low carbonpercentage by weight alumina-based polybutadienespherical particale, 5μm in diameter with a porediameter of 80Å.L20Dihydroxypropane groups chemically bonded toporous silica particles, 3~10μm in diameter.L30Ethyl silane chemically bonded to a totally poroussilica particle, 5~10μm in diameter.071HPLC ColumnsL21L22BrandVenusil DiolBrandsphereA rigid, spherical styrene-divinylbenzene copolymer,5~10μm in diameter.AKF RPBrandsphereA cation-exchange resin made of porous polystyrenewith sulfonic acid groups, 5~10μm in diameter.AKF CISLHParticle(μm)5Particle(μm)5, 7Particle(μm)7FigureFigureFiguresphereL31L32L33L34A strong anion-exchange resin-quaternary aminebonded on latex particles attached to a core of 8.5μmmacroporous particles having a pore size of 2000Åand consisting of ethylvinylbenzene cross- linked with55% divinyl benzene.A chiral-ligand exchange packing-L proline/coppercomplex covalently bonded to an irregularly shapedsilica particles, 5~10μm in diameter.Packing having the capacity to separate proteins of4000 to 40000 daltons. It is spherical, silica-basedand processed to provide pH stabitlity.Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in thelead form, about 9μm in diameter.L23A n a n i o n e x c h a n g e r e s i n m a d e o f p o r o u spolymethacrylate or polyacrylate gel with quaternaryammonium groups, about 10μm in diameter.L35Zirconium-stabilized spherical silica packing with ahydrophilic (diol-type) molecular mono layer bondedphase having a pore size of 150Å.L24A semi-rigid hydrophilic gel consisting of viny lpolymers with numerous hydroxyl groups on thematrix surface, 32~63μm in diameter.L363,5-dinitrobenzoyl derivative of L-phenylglycinecovalently bonded to a 5μm aminopropyl silica.L25Packing having the capacity to separate compoundswith a molecular weight range from 100 to 5000 (asdetermined by polyethylene oxide), applied to neutral,anionic and cationic water-soluble polymers. Apolymethacrylate resin base, cross-linked with polyhydroxylatedether surface contained some residualcarboxyl groups was found suitable.L37L38Packing having the capacity to separate proteins bymolecular size over a range of 2000-40000 daltons. Itis a polymethacrylate gel.A methacrylate-based size-exclusion packing forwater soluble samples.L26Butyl silane (C4) chemically bonded to porous silicaparticles, 5~10μm in diameter.L39A hydrophilic-polyhydroxy methacrylate gel of totallyporous spherical resin.BrandVenusil XBP C4Particle(μm)3, 5Figuresphere

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersL40Cellulose tris 3,5-dimethylphenyl carbamate coatedporous silica particles, 5~20μm in diameter.L51Amylose tris-3,5-dimethylphenyl carbamate-coated,porous, spherical, silica particles, 5 to 10μm indiameter.BrandChiral OD-HParticle(μm)5FiguresphereBrandChiral AD-HParticle(μm)5FigureIrregularL41Immobilized α1-acid glycoprotein on spherical silicaparticles, 5μm in diameter.L42Octylsilane and octadecylsilane groups chemicallybonded to porous silica particles, 5μm in diameter.BrandOptimix C18/C8Particle(μm)3, 5FiguresphereL43Pentafluorophenyl groups chemically bonded to silicaparticles, 5~10μm in diameter.BrandVenusil PFPParticle(μm)5FiguresphereL44L45L46A multifunctional support, which consists of a highpurity, 60Å, spherical silica substrate that has beenbonded with a cationic exchanger, sulfonic acidfunctionality in addition to a reversed- phase C8functionality.Beta cyclodextrin bonded to porous silica particles,5~10μm in diameter.Polystyrene/divinylbenzene substrate agglomeratedwith quaternary amine functionalized latex beads,10μm in diameter.072HPLC ColumnsBrandAKF QLVParticle(μm)5, 7FiguresphereL47L48L49L50High capacity anion-exchange microporous substrate,fully functionalized with a trimethylamine group, 8μmin diameter.Sulfonated, cross-linked polystyrene with anouter layer of submicron, porous, anion-exchangemicrobeads, 15μm in diameter.A reversed-phase packing made by coating a thinlayer of polybutadiene on to spherical porous zirconiaparticles, 3 to 10μm in diameter.Multifunction resin with reversed-phase retentionand strong anion-exchange functionalities. The resinconsists of ethylvinylbenzene, 55% cross-linked withdivinylbenzene copolymer, 3 to 15μm in diameter, anda surface area of not less than 350m 2 /g, substrateis coated with quaternary ammonium functionalizedlatex particles consisting of styrene cross-linked withdivinylbenzene.

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYApplications1.Antibiotics1.1 Benzylpenicillin SodiummAU300Benzylpenicillin Sodium2502001501005000 2.5 5 7.5 10 12.5 15minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: 0.1mol/L potassium dihydrogen phosphate buffer(pH=2.5):ACN=70:30Detection: UV 225nmFlow Rate: 1mL/minInjection: 4μL1.2 Amoxicillin SodiummAU80 Amoxicillin Sodium073HPLC Columns6040200HOH 2NHONHOOHHNON a-200 2 4 6SHCH 3CH 3minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Phosphate buffer(0.05mol/L, pH=5.0):ACN=97.5:2.5Detection: UV 254nmFlow Rate: 1mL/minInjection: 5μL1.3 CefradinemAUCefradine350300250H 2 NH2001501005000 5 10 15OONHHHONminSOHCH 3Column: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:MeOH:3.86% sodiumacetate solution/4% acetic acid solution=742:240:15:3Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers1.4 CefaclormAU706050403020100CefaclorH 2 NH0 2 4 6 8 10 12 minOONHHHONSOHCl,H 2 OColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: 0.05mol/L phosphatebuffer(pH=3.4):ACN=92:8Detection: UV 254nmFlow Rate: 1mL/minInjection: 5μL1.5 CefadroxilmAU2502001501005000 2 4 61.6 CefalexinNorm.100 Cefalexin806040200H 2NHO OHO ONHHHNSCH3,H 2O0 2 4 6 8 10minminColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: 0.05mol/L phosphatebuffer(pH=5.5):ACN=96:4Detection: UV 230nmFlow Rate: 1mL/minInjection: 2μLColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:MeOH:3.86%sodium acetate solution/4%acetic acid solution=742:240:15:3Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL074HPLC Columns1.7 Cefotaxime SodiumNorm.80 Cefotaxime Sodium706050403020100H 2NNSOONHN HHOCH3OONSNaOOC H 30 2 4 6 8 10 12 14 minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: phosphate buffer(0.4mMKH 2 PO 4 +8mM K 2 HPO 4 )/MeOH=89:11Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY1.8 Sulbactam Sodium + Cefoperazone SodiummAUOCefoperazone Sodium500NaOOH400N CH3300S CH3OH OSulbactam Sodium20010000 2.5 5 7.5 10 12.5 15 minColumn: Venusil XBP C18, 4.6×250mm, 5μmPart No.: VX952505-0Mobile Phase: 0.005 mol/L TABOH (adjust pH=5.0with H 3 PO 4 ):ACN=70:30Detection: UV 220nmFlow Rate: 1mL/minInjection: 2μL1.9 AcetylspiramycinmAUOON075HPLC Columns14012010080604020OO001 02 03 04 05 06 0m1.10 MeleumycinOOHOHOO109876543210-1051 01 52 02 53 03 54 04 55 05 56 06 57 01.2409.31511.21518.61520.64829.98249.04852.248O57.315NOOHOOHColumn: Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: ACN:NaClO4/H 3 PO 4 (pH=2.2)=30:70Temperature: 30℃Flow Rate: 0.8mL/minInjection: 20μLColumn: Venusil XBP C8, 4.6×150mm, 5μmPart No.: VX851505-0Mobile Phase: ammonium formate (0.2mol/L, adjust topH=7.3 with TEA):ACN=62:38Detection: UV 232nmFlow Rate: 1mL/minTemperature: 30℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers2.Synthetic Antimicrobial Agents2.1 NorfloxacinmAU200Norf loxacin1501005000 2. 5 5 7. 5 10 12 .52.2 Ofloxacinmi nColumn: Venusil XBP C18, 4.6×250mm, 5μmPart No.: VX952505-0Mobile Phase: H 3 PO 4 (0.025mol/L,adjust to pH=3.0 withTEA):ACN=87:13Detection: UV 278nmFlow Rate: 1mL/minInjection: 20μLmAU800700H 3CH600NOCH 3500NN400300FCO OH200O10000 2 4 6 8 102.3 Ciprofloxacin LactatemAUminColumn: Venusil XBP C18, 4.6×250mm, 5μmPart No.: VX952505-0Mobile Phase: NH 4 AC/KClO 4 (40mM, pH=2.0):ACN=85:15Detection: UV 294nmFlow Rate: 1mL/minInjection: 4μL076HPLC Columns200175Ciprofloxacin LactateHN150NN125100FCO OH75O502500 1 2 3 4 5 6 7minColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: Citric acid buffer (0.05mol/L, adjust to pH=3.5with TFA):ACN=82:28Detection: UV 277nmFlow Rate: 1mL/minInjection: 10μL

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY2.4 Gatifloxacin hydrochloride06060402006.988N=8763.37As=1.3905 10 15 20 25 30H NFNOONOO HH C l040200Column: Venusil XBP C18(L), 4.6×150mm, 5μmPart No.: VX951505-LMobile Phase: 1%TEA(pH=4.5):ACN=87:13Detection: UV 325nmFlow Rate: 1.5mL/minInjection: 10μLTemperature: 40℃2.5 FluconazolemAU300Fluconazole2500772001501005000 1 2 3 4 5 6 7minColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: KH 2 PO 4 buffer(adjust to pH=7.0with NaOH):MeOH=55:45Detection: UV 260nmFlow Rate: 1mL/minInjection: 10μLHPLC Columns3.Anti-virus Medicine3.1 RifampicinmAU250200150100500H 3 CH 3 COOH 3 CH 3 COO HC H O3O H O HH 3 CC H 3OOOC H 3N HO HO HRifampicin0 5 10 15 20C H 3NNNC H 3minColumn: Venusil XBP C8, 4.6×250mm, 5μmPart No.: VX852505-0Mobile Phase: MeOH/ACN/KH2PO4 0.075mol/L/citric acid1mol/L=30:30:36:4Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers3.2 GanciclovirNorm.Ganciclovir120 O1008060HNH 2 N NNN OOH40OH2000 2 4 6 8 10 12 14 minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:MeOH=95:5Detection: UV 252nmFlow Rate: 1mL/minInjection: 2μL4. Steriod Hormones4.1 EthinylestradiolNorm.Ethinylestradiol10008006004002000HOHHHOHCH 3CCH0 1 2 3 44.2 Triamcinolone AcetonideminColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: MeOH:Water=70:30Detection: UV 281nmFlow Rate: 1mL/minInjection: 2μL078HPLC ColumnsNorm.40035030025020015010050OH OO HHC H 3 HFOC H 3C H 3 O C H 3OHHTriamcinolone Acetonide00 2 4 6 8 minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: MeOH:Water=21:19Detection: UV 240nmFlow Rate: 1mL/minInjection: 2μL

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY4.3 HydrocortisoneNorm.Hydrocortisone200OOHCH 3HO H OH150CH 3 H100HHO5000 1 2 3 4 5 6 7minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: MeOH:Water=70:30Detection: UV 240nmFlow Rate: 1mL/minInjection: 10μL4.4 DexamethasoneNo rm .079HPLC Columns20 017 515 012 510 07550250Norm.200150100500OOH H 3 CH OC H 3 HFDexamethasone0 2 4 6 84.5 LevonorgestrelO HHC H 3250 Levonorgestrel0 2 4 6 8O HOHH 3 CHHHO HCC Hmi nminColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: Citric acid buffer (0.05mol/L, adjust to pH=3.5with TFA):ACN=82:28Detection: UV 277nmFlow Rate: 1mL/minInjection: 10μLColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:ACN=30:70Detection: UV 240nmFlow Rate: 1mL/minInjection: 2μL4.6 PrednisoneColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:THF:MeOH=668:250:62Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersNorm.Prednisone250O OHCH 3 OH200OCH 3 H150HH100O5000 2 4 6 8 10 12 14 minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Water:THF:MeOH=668:250:62Detection: UV 254nmFlow Rate: 1mL/minInjection: 2μL4.7 Clobetasol PropionateNorm.70 Clobetasol Propionate6050403020100OHOHCH 3FHOCH 30 2 4 6 8HOHClCH 3OCH 3minColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Phosphate buffer 0.05mol/L, pH=2.5/ACN/MeOH=425:475:100Detection: UV 240nmFlow Rate: 1mL/minInjection: 2μL4.8 FluocinonidemAU400FluoxinonideO350O C H 3300OC H250O H C H 33HO200C H 3 H OC H 3H150F H100O500F H0 1 2 3 4 5 6m inColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: MeOH:ACN:Water=60:10:30Detection: UV 240nmFlow Rate: 1mL/minInjection: 10μL080HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY5 Medicine for Gastric Ulcer5.1 Ranitidine HydrochloridemAU250 Ranitidine Hydrochloride200150100H 3CNCH 3OSHNHNCH 3NO 2HClColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: MeOH:0.77% Ammonium acetate aq.=285:11550Detection: UV 320nm00 1 2 3 4 5minFlow Rate: 1mL/minInjection: 10μL5.2 CimetidinemAU500 Cimetidine400300200H NNC H 3SHNNHNC H 3C H 3Column: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: MeOH:Water:H 3 PO 4 :TFA=200:800:0.3:0.2081HPLC Columns1000mAU1750 1 2 3 4 5 6 75.3 OmeprazoleOmeprazol emi nDetection: UV 220nmFlow Rate: 1mL/minInjection: 10μL1501251007550250C H 3ONHNSONC H 3O C H 30 2 4 6 8 10C H 3minColumn: Venusil XBP C8, 4.6×250mm, 5μmPart No.: VX852505-0Mobile Phase: NaH 2 PO 4 Buffer (0.01 mol/L, adjust topH7.6 with H 3 PO 4 ): ACN=60:40Detection: UV 302nmFlow Rate: 1mL/minInjection: 4μL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers6. Analysis of Alkaloids6.1 Quaternary Ammonium Alkaloids from CoptidisAU0.250.200.150.100.050.000.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 55.00 60.00Column: Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: Water(0.3%TEA):ACN=75:25Detection: UV254nmFlow Rate: 1mL/minTemperature: 25℃6.2 MatrineVWD1 A Wavelength=220nm(NEWSHIYP004.D)mAU809.04260402003.29505 10 15 20 25 min6.3 Ephedrine from CoptidisColumn: Venusil XBP NH 2 , 4.6×250mm, 5μmPart No.: VN852505-0Mobile Phase: ACN:Ethanol:3% H 3 PO 4 aq.=80:10:10Detection: UV 220nmFlow Rate: 1mL/minInjection: 20μL082HPLC ColumnsmAU25VWD1 A, Wavelength=210 nm (F:\GTQWJ\MH000045.D)201510Ephedrina hydrochloridum500 5 10 15 20 25 30minmAU17.5VWD1 A, Wavelength=210 nm (YXN\07091821.D)1512.5Ephedrina hydrochloridum107.552.50-2.50 5 10 15 20 25 30minMobile Phase: ACN:SDS/H 3 PO 4 =40:60Detection: UV 210nmFlow Rate: 1mL/min

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY6.4 Berberine Hydrochloride From PhellodendronVWD1 A, Wavelength=346 nm (TF\XBJ00079.D)mAU7060Berberine hydrochloride50403020CH 3OCH 3O+NOCl - , 2H 2OO1000 2.5 5 7.5 10 12.5 15 17.5minmAUVWD1 A, Wavelength=346 nm (TF\XBJ00072.D)120100Berberine hydrochloride8060402000 2.5 5 7.5 10 12.5 15 17.5minMobile Phase: ACN:1%TFA=35:65Detection: UV 346nmFlow Rate: 1mL/min6.5 Aconitine, Mesaconitine and Hypaconitin083HPLC ColumnsmAU250200150100500-50VWD1 A, Wavelength=235 nm (WUTOU\ZHENGJ26.D)mesaconitineaconitinehpyaconitine0 10 20 30minColumn: Promosil C18, 4.6×250mm, 5μmPart No.: PM952505-0Mobile Phase: ACN: 2% Acetate Buffer adjust pH=6.5with TEA=15:85Detection: UV 235nmFlow Rate: 1mL/min6.4 Berberine from PhellodendronmAU100VWD1 A, Wavelength=238 nm (YXN\07091805.D)Morphine80604020Codeine phosphate00 2.5 5 7.5 10 12.5 minColumn: Venusil XBP C18(2), 4.6×250mm, 5μmPart No.: VX952505-2Mobile Phase: MeOH: CH 3 COONa aq. (0.03mol/L,adjust pH to 3.5 with Acetic acid)=15:85Detection: UV 238nmFlow Rate: 1mL/min

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers7.Agricultural Chemical7.1 Analysis of 14 Herbicides0.00400.00350.00300.00250.0020A 0.00150.00100.00050.0000-0.00050.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00Sample: dazomet Cymoxanil Triadimefon TebuconazoleThiophanate-Methyl hexaconazole flutriafolIprodione Metalaxyl Procymidone carboxinProchlora Diethofencar Triadimenol1,2,3,6-tetrahydro-N-(trichloromethylthio)phthalimideColumn: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: A: Ammonium acetate buffer; B:ACNGradient: 40%B to 45%B in 8 min, hold for 40min.Detection: UV 225nm Flow Rate: 1mL/minInjection: 20μL Temperature: 25℃7.2 Haloxyfop-P-methyl0.80.70.60.50.4AU0.30.20.10.02.0 4.0 6.0 8.0 10.0 12.0 14.0 16.07.3 Fluazifop-p-butylAU1. 2Column: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: ACN:Water=70:30Detection: UV 225nmFlow Rate: 1mL/minInjection: 5μLTemperature: 25℃084HPLC Columns1. 00. 80. 60. 40. 20. 02. 0 4.0 6. 0 8. 0 10 .0 12 .0 14 .0 16 .0 18 .0 20 .0Column: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: ACN:Water=70:30Detection: UV 225nmFlow Rate: 1mL/minInjection: 5μLTemperature: 25℃7.4 1,2-Dibenzoyl-1-tert-butylhydrazine0. 800. 60AU 0. 400. 200. 001. 00 2. 00 3. 00 4. 00 5. 00 6. 00 7. 00 8. 00 9. 00 10 .0 0Column: Venusil XBP C18, 4.6×150mm, 5μmPart No.: VX951505-0Mobile Phase: Water:MeOH=25:75Detection: UV 254nmFlow Rate: 1mL/minInjection: 10μLTemperature: 25℃

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY8. Analysis of Amino AcidsColumn: Venusil AA, 4.6×250mm, 5μmPart No.: AA952505-0Mobile Phase: A: 0.1mol/L CH 3 COONa (Adjust pH=6.5 with Acetic Acid):Acetonitrile (93:7)B:Water: Acetonitrile (4:1)Gradient:Time(min)%B0011713.9121415293429.11003710037.10450Flow Rate: 1mL/minDetection: UV 254nmTemperature: 40℃Sample: PTC-AA (derivatization of amino acids with PITC)8.1 Standards of Amino Acids085HPLC Columns8.2 Amino Acids from Salmon Calcitonin

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers8.3 Amino Acid from TerlipressinSampleStandards of Amino Acids9. Applications in LC-MS9.1 Pseudoephedrine in Plasma086HPLC Columns2.8e52.6e52.4e52.2e52.0e51.8e51.6e51.4e51.2e51.0e58.0e46.0e44.0e42.0e40.0Pseudoephedrine0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min2.124.0e53.5e53.0e52.5e52.0e51.5e51.0e50.5e51.47Lignocaine0.20 .4 0.60 .8 1.01 .2 1.41 .6 1.82 .0 2.22 .4 2.62 .8 3.03 .2Time.minMass System: API Qtrap 3200 Applied BiosystemMass Condition: Cation Detector Mode, MRMHPLC Column: Venusil ASB C18, 2.1mm×50mm; Part No.:VS950502-0;Sample: Prepared sample by SPE(Cleanert PCX)Mobile Phase: A:1% Formic Acid in Water; B:MethanolGradient: 20%B to 95%B in 2 min, hold for 0.5 min, then switch to A:B (20:80)Flow Rate: 0.5mL/minTemperature: 25℃

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY9.2 Oleic Acid in PlasmaOHOOleic acid49921.44Intensity.cps40000300020001000Intensity.cps01.4e61.2e61.0e68.0e56.0e54.0e52.0e505.0e50.51 .0 1.52 .0 2.53 .0 3.54 .0 4.55 .0 5.56 .0Time.min3.430.51 .0 1.52 .0 2.53 .0 3.54 .0 4.55 .0 5.56 .0Time.min4.56087HPLC ColumnsIntensity.cps4.0e53.0e52.0e51.0e500.51 .0 1.52 .0 2.53 .0 3.54 .0 4.55 .0 5.56 .0Time.minMass System: API Qtrap 3200 Applied BiosystemMass Condition: Anion Detector Mode, MRMHPLC Column: Venusil ASB C18, 2.1mm×50mm; Part No.: VS950502-0Sample: Prepared sample by SPE (Cleanert PEP)Mobile Phase: A:13mmol/L ammonium acetate aq.; B:AcetonitrileGradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B(95:5) then hold for 2 min.Flow Rate: 0.8 mL/minTemperature: 25℃

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers10. Others10.1 GlipizideN =7587AS=1.03Column: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: Phosphate Buffer 0.1 mol/L,pH 6.0:MeOH=55:45Flow Rate: 1mL/minInjection: 20μL10.2 TerfenadineN=82 49As=1.0 010.3 Dopamine and Its MetabolinColumn: Unisol C18, 4.6×150mm, 5μmPart No.: UO951505-0Mobile Phase: MeOH:H3PO4/Triethylamine(0.1mol/L)=80:20Detection: UV 254nmFlow Rate: 1mL/minInjection: 10μL086HPLC ColumnsDopamineIsoprenalineIntens.mAU6005101409 .D: UV Chromatogram, 280 nm402000 5 10 15 20 25 30 35 40 Ti me [mi n]Sample: Dopamine, Isoprenaline, SoprenalineColumn: Venusil PFP, 4.6×250mm, 5μmPart No.: VF952505-0Mobile Phase: MeOH:CH 3 COOH/CH 3 COONH 4Buffer(pH 4.5)=15:85Detection: UV 280nmFlow Rate: 0.8mL/min

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY10.4 Organic AcidsCH 3StandardSample: Formic acid, acetic acid, maleic acid,succinic acid, fumaric acidColumn: Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: 0.02M NH 4 AC Aq.:MeOH=95:5Detection: UV 215nmFlow Rate: 1mL/minInjection: 10μLTemperature: 20℃10.5 Catechins in Leaves10 0089HPLC ColumnsVo ltsVo lts0. 50. 40. 30. 20. 1120. 0800 5 10 15 20 25 30 35 40 45 50Mi nu te s10.6 Saccharin Sodium in Milk Powder0. 150. 100. 0512340. 00800. 0 2. 5 5. 0 7. 5 10 .0 12 .5 15 .0 17 .5 20 .0 22 .5 25 .0 27 .5 30 .0Mi nu te s959085Pe rc en t10 0959085Pe rc entSample: Extract of leavesColumn: Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: 0.02%H 3 PO 4 :MeOH=81:19Detection: UV 278nmFlow Rate: 1mL/minTemperature: 40℃Sample: Benzoic acid, 2,4-Hexadienoic acid, Sodiumsaccharine, milk powderColumn: Unisol C18, 4.6×250mm, 5μmPart No: UO952505-0Mobile Phase: MeOH:CH 3 COONH 4 (0.02 M)=5:95Detection: UV 230nmFlow Rate: 1mL/minTemperature: 30℃10.7 DHA in the Health FoodColumn: Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: MeOH:Water=80:20Detection: UV 254nmFlow Rate: 1mL/minInjection: 10µL

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit Customers10.8 Tartrazine in FoodColumn:Unisol C18, 4.6×250mm, 5μmPart No.: UO952505-0Mobile Phase: A: Ammonium acetatebuffer pH 4.0; B:MeOHGradient: 35%B to 50%B in 5 min.Detection: UV 254nmFlow Rate: 1mL/minInjection: 10µL10.9 Melamine in Feed10.10 Tetrodotoxin(TTX)mA121086420H NNHNHH OHH OHO HO -HHOOHHC H 2 O HO H12 34 56 78 9m lColumn: Venusil ASB C18, 4.6×250mm, 5μmPart No.: VS952505-0Mobile Phase:10mM Citric acid+10mMPerfluorooctane sulfonate(pH=3.0):ACN=85:15Detection: UV 240nmFlow Rate: 1mL/minInjection: 10µLTemperature: 40℃Column: Venusil ASB C18, 4.6×250mm, 5μmPart No.: VS952505-0Mobile Phase: 0.02% H 3 PO 4 :MeOH=40:60Detection: UV 200nmFlow Rate: 0.5mL/minTemperature: 25℃090HPLC Columns10.11 Baicalin1000.15H OO HO750.10OO0.05O HOH OO HO O H0.000.02 .5 5.07 .5 10 12.51 5 17.52 0 22.52 5 27.53 05025Column:Venusil ASB C18, 4.6×150mm, 5μmPart No.: VS951505-0Mobile Phase: MeOH:1% acetic acid=50:50Detection: UV 274nmFlow Rate: 1mL/minInjection: 5μL

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHY10.12 2 - piperazine-N-(2,6-dimethylphenyl) acetamideTime(min)0152540A (%) B (%)9090404010106060Conditions:Column: Durashell-C18, 4.6×250mm, 5µm, 100ÅMobile phase: A: Phosphate buffer (pH=7.0)B: AcetonitrileFlow rate: 1.0mL/minDetection: UV220nmTemperature: 30℃Injection: 5μL10.13 Dyes归 一 化VWD1 A, Wavelength=280 nm ( 张 莹 \20110221000009 .D)6009150403.1194.6155.552Conditions:HPLC Columns30202.4103.8157.2648.6629.4031000 2 4 6 8 10 12 14minColumn: Durashell-C18, 4.6×150mm, 5µm, 100ÅMobile phase: 10% TEOA (pH=7.7) : Acetonitrile = 95:5Flow rate: 1.0mL/minDetection: UV280nmTemperature: 20℃Injection: 2μL1: p- Phenylenediamine; 2: 4-Aminophenol;3: methylbenzene 2,5Phenylenediamine;4: Hydroquinone; 5: m-Aminophenol; 6: o-Phenylenediamine;7:1, 4 Phenylenediamine; 8: p- methylaminophenol.

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556Best ValueGuaranteed Product QualityInnovation to Benefit CustomersAPPENDIXColumn Cleaning ProceduresDue to interactions between the stationary phase andsample components, HPLC columns may occasionallyrequire cleaning or regeneration. The following conditionsapply to silica-based columns. Flow rates should be1/5-1/2 of the typical flow rate.To estimate the column volume, use the followingequation:V=πr 2 ×LV = Column Volume in mLr = Column Radius in cmL = Column Length in cmUNBONDED SILICA COLUMNS (SILICA)REVERSED PHASE COLUMNS(C18, C8, C4, C2, C1, PHENYL, CN and NH 2 )REVERSED PHASE PROTEIN/PEPTIDECOLUMNS (C18, C8, C5, C4 and PHENYL)BONDED NORMAL PHASE COLUMNS(CN, NH 2 and DIOL)GFC/SEC COLUMNS FOR PROTEINSION-EXCHANGE COLUMNS(SAX, SCX, NH 2 and DEAE)Rinse with 10 column volumes each of: Hexane, Methylene Chloride,Isopropanol, Methylene Chloride. Mobile phase: Flush column with 30mL2.5% 2,2-dimethoxy- propane and 2.5% glacial acetic acid in hexaneRinse with 10 column volumes of: 95% Water/5% Acetonitrile (for bufferremoval) followed by 95% Acetonitrile/5% Water mobile phaseRinse with 20 column volumes of mobile phase with buffer removed rungradient (2x):A) 0.1% Aqueous TFA in WaterB) 0.1% TFA in Acetonitrile/Isopropanol (1:2) 25% B to 100% B for 30 minutesEquilibrate with 10 column volumes of mobile phase.Rinse with 10 column vol umes each of: Chloroform, Isopropanol, Methylenechloride, mobile phase. Exception: Recommended for cleaning Aminowhen used in reversed phase mode:1. Wash with at least 30 column volumes of Sodium Hydroxide (pH=11.0)2. Flush with at least 30 column volumes of Water (HPLC grade)3. Re-equilibrate to Mobile phase conditions.(300X7.8mm size columns) Rinse with 5 column volumes of:0.1M Phosphate buffer pH=3.0. For strongly retained proteins: Run 100%Water to 100% Acetonitrile to 100% Water over 60 minutes or wash with5 column volumes of SDS or 6M Guanidine Thiocyanate or 10% DMSORinse with 10 column volumes of:500mM Phosphate buffer pH=7.0 10% Acetic acid (Aq)5 Column Volumes of Water10 Column Volumes of Phosphate buffer pH=7.05 Column Volumes of Water10 Column Volumes of Methanol10 Column Volumes of WaterFor protein removalFollow the above procedure with this exception: Substitute 10 columnvolumes of Methanol with 10 column volumes of 5M Urea or 5MGuanidine Thiocyanate092HPLC Columns

Bonna-AgelaTechnologiesBETTER SOLUTIONS FOR CHROMATOGRAPHYThe Methods of Maintaining Good Column Lifetime and Performance● Inject only well-prepared (filtration, liquid/liquid extraction, SPE) clean samples● Minimize pressure surge; avoid mechanical and thermo shock● Use guard columns or on-line filtration● Flush columns frequently using an appropriate program● Remove unstable and strongly retained components of no-interest from samples● Use low pH (1.0-6.0) mobile phase if possible● Use organic buffer when operating at medium to high pH (6.0-10.0)● Avoid elevated temperature unless it is necessary● Add 200 ppm sodium azide in aqueous mobile phase to suppress the growth of the bacteria● Wash out all buffer salts and store the columns in high organic solution for overnight or long time storage093HPLC Columns