Fisher Chemical

Molecular Biology | Electrophoresis of Nucleic AcidsEnzymes (Modifying) | Molecular Biology250 Base-Pair LadderDNA-Hind III/phi X-174 RF DNA-Hae III DigestpackagingMfr. No200 µg PolyMicroTube BP2552-100packagingMfr. No25 µg PolyMicroTube BP2555-25Applications: For precise sizing of double-stranded DNA fragments from250-2000 bp on agarose gels.Supplied at 1µg/µÒ in TE buffer (pH 7.5).Consists of multiple repeats of a 250 bp fragment.Not designed for quantifying DNA content in a sample.Recommended Gel: 1% agarose with loading amount of 2.0µg/lane.Do not heat before loading.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.Applications: For sizing DNA fragments from 72 to 23.130 bp on agarose gels.Contains a combination of lambda DNA and phi X-174 RF DNA that has beendigested with restriction enzyme.Recommended Gel: 1% agarose with loading amount of 0.5-1.0µg/lane.Heat before loading at 60°-65°C for 2 minutes.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.50 Base-Pair LadderpackagingMfr. No200 µg PolyMicroTube BP2550-100DNA-Hind III/phi X-174 RF DNA-Hae III DigestLyophilized PowderpackagingMfr. No25 µg PolyMicroTube BP2554-25MFR No.MFR No.Applications: For precise sizing of average-sized PCR products and other DNAsfrom 50-500 bp on agarose gels.Supplied at 1µg/µÒ in TE buffer (pH 7.5).Consists of multiple repeats of a 50 bp fragment.Can be visualized by ethidium bromide staining or by autoradiography (afterradiolabeling).Recommended Gel: 2% agarose/ 6% polyacrylamide with loading amount of2.0µg/lane.Do not heat before loading.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.Applications: For sizing DNA fragments from 72 to 23.130 bp on agarose gels.Contains a combination of lambda DNA and phi X-174 RF DNA that has beendigested with restriction enzyme.Recommended Gel: 1% agarose with loading amount of 0.5-1.0µg/lane.Heat before loading at 60°-65°C for 2 minutes.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.Kilobase DNA MarkerpackagingMfr. No50 µg PolyMicroTube BP2553-100Ø X-174 RF DNA-Hae III DigestpackagingMfr. No10 µg PolyMicroTube BP2558-10100 µg PolyMicroTube BP2558-100Applications: For quick and precise sizing of double-stranded DNA fragmentsfrom 500 bp to 10.0kb on agarose gels.Supplied at 0.5µg/µÒ in TE buffer (pH 8.0).Consists of 11 easily identifiable bands - 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0,6.0, 8.0, and 10.0kb - when run on a 0.8% agarose gel.Not designed for quantifying DNA content in a sample.Recommended Gel: 0.8% agarose with loading amount of 0.5µg/lane.Do not heat before loading.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.Applications: For sizing DNA fragments from 72 to 1.353 bp on agarose gels.Recommended Gel: 1% agarose with loading amount of 0.5µg/lane.Do not heat before loading.Contains phi X-174 RF DNA that has been digested with restriction enzyme.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.MFR No.MFR No.DNA-Hind III DigestpackagingMfr. No100 µg PolyMicroTube BP2556-200Collagenase From Clostridium histolyticumYellowish Brown Lyophilized PowderIslet Isolation GradepackagingMfr. No1 g AmberGlass BP2649-1CAS: 9001-12-1Applications: For sizing DNA fragments from 125 to 23.130 bp on agarose gels.Recommended Gel: 1% agarose with loading amount of 0.5µg/lane.Contains lambda DNA that has been digested with restriction enzyme.Recommended Storage: -20°CNot on TSCA inventory: for R and D use only; not for manufacturing orcommercial purposes.Activity>=3.500units/gApplications: Collagenase is most suitable for pancreatic islet isolation.Unit Definition: One unit will liberate peptides from collagen equivalent inninhydrin color to 3.0µmole of L-leucine in 18 hr. at pH 7.4 and 37°C.Inhibitors:Hg 2+ , Pb 2+ , Cd 2+ , Cu 2+ , Zn 2+ , EDTA, o-phenanthroline.Recommended Storage: 0° to 5°C, desiccate394395