ACE Super C18 Brochure - Winlab.com.au

ACE SuperC18 Ultra-Inert UHPLC and HPLC Columns with Extended pH StabilityEncapsulated Bonding Technology (EBT) for Improved Chromatography and StabilityTraditional C18 BondingACE SuperC18 with Encapsulated Bonding Technology (EBT)C18Endcapping groupUnbonded silanol groupC18■■■The unique Encapsulated Bonding Technology (EBT) developed for ACE SuperC18 columns dramatically increasesligand coverage of the silica surface and effectively eliminates the effect of unbonded silanol groups from separations.This higher ligand coverage results in improved inertness, chromatographic performance and stability.Under aggressive acidic conditions (see Figure 1) ACE SuperC18 is highly resistant to ligand cleavage, due to acombination of the Encapsulated Bonding Technology (EBT) and ultra-inert ACE silica. Many C18 bonded columnswill exhibit ligand cleavage under acidic conditions, resulting in retention shifts and/or increased peak tailing.Under basic conditions with LC/MS compatible mobile phases (see Figure 2), the Encapsulated Bonding Technology(EBT) shields the ACE SuperC18 surface from dissolution whilst maintaining excellent chromatographic performance.Traditional C18 bonded silica columns are prone to silica dissolution under such aggressive conditions, which can resultin premature column deterioration.Figure 1 - Excellent Acidic Stability at pH 1.8Application # 1511Acidic flow conditionsColumn: ACE Excel 2µm SuperC18, 50 x 2.1mmMobile Phase: 50:50 MeOH/0.1% TFA in H 2 O (pH 1.8)Temperature: 40°C Flow Rate: 0.20ml/minFigure 2 - Excellent Basic Stability at pH 10.7Application # 1512Basic flow conditionsColumn: ACE Excel 3µm SuperC18, 150 x 4.6mmMobile Phase: 50:50 MeCN/0.1% NH 3 in H 2 O (pH 10.7)Temperature: 40°C Flow Rate: 1.00ml/minDay 11 2k (phenanthrene) = 16.91Day 112N0.5 (biphenyl) = 169,100pl/m3453450 1 2 3 4 5 6 7 8 9 10 11 12 13 140 1 2 3 4 5 6 7 8 9 10 11 12 13 14Acidic Mobile Phase (pH 1.8)(continuous flow)>20,000 column volumes>2,000 injectionsBasic Mobile Phase (pH 10.7)(continuous flow)>20,000 column volumes>2,000 injectionsDay 301 234k (phenanthrene) = 16.855Day 30123N0.5 (biphenyl) = 168,700pl/m450 1 2 3 4 5 6 7 8 9 10 11 12 13 14Time - MinutesEvaluation conditionsSample: 1) uracil 2) dimethyl phthalate 3) toluene 4) biphenyl 5) phenanthreneMobile Phase: 70:30 MeOH:H 2 OTemperature: 22°C Flow Rate: 0.20ml/min Wavelength: 254nm0 1 2 3 4 5 6 7 8 9 10 11 12 13 14Time - MinutesEvaluation conditionsSample: 1) uracil 2) dimethyl phthalate 3) toluene 4) biphenyl 5) phenanthreneMobile Phase: 80:20 MeOH:H 2 OTemperature: 22°C Flow Rate: 1.00ml/min Wavelength: 254nm2www.ace-hplc.comAustralia & New Zealand contact: infor@winlab.com.au or call +61 7 3205 5233

ACE SuperC18 Ultra-Inert UHPLC and HPLC Columns with Extended pH StabilityComparison of Column Inertness at Intermediate pH■ Leading column brands in 50 x 2.1mm LC/MS compatible dimensions at pH 5.8■ Silica, Hybrid and Superficially Porous particle technologies compared■ Comparison of column efficiency for pyridine, a basic molecule■ Efficiency measured at 5% peak height to account for peak tailing effectsPeak Efficiency ComparisonReproduced with kind permission of The Open University, UK.Application # 1513ACE Excel 2 SuperC18Zorbax Eclipse 1.8 XDB C1842,400pl/m68,900pl/mLower TailingAgilent Poroshell 2.7 120 EC C1841,900pl/mWaters XBridge 2.5 BEH C18 XP30,800pl/mWaters Acquity 1.7 BEH C1830,700pl/mPhenomenex Kinetex 2.6 C1826,800pl/mWaters XBridge 2.5 C1825,600pl/mPhenomenex Luna 2.5 C18(2) HST a21,900pl/mNucleoshell 2.7 RP18 a21,200pl/mPhenomenex Kinetex 1.7 C1820,700pl/mZorbax Extend 1.8 C18Thermo Accucore 2.6 C1810,300pl/m7,300pl/mHigher Tailing0 20,000 40,000 60,000 80,000 100,000N0.05 (pyridine) (plates/m)ACE Excel 2 SuperC18(fully porous ultra-inert silica)N 0.05 (pyr) = 68,900pl/mWaters ACQUITY 1.7 BEH C18(hybrid particle)N 0.05 (pyr) = 30,700pl/mPhenomenex Kinetex 1.7 C18(core-shell particle)N 0.05 (pyr) = 20,700pl/m2223313110 1 2 3 4 5 0 1 2 3 4 5 0 1 2 3 4 5Time - Minutes Time - Minutes Time - MinutesColumn Dimensions: 50 x 2.1mm ( a 50 x 2.0mm) Sample: 1) uracil 2) pyridine 3) phenol Mobile Phase: 30:70 MeOH/10mM NH 4 OAc in H 2 O (pH 5.8)Flow Rate: 0.20ml/min Temperature: 22°C Wavelength: 254nmComparative data may not be representative of all applications. Please see page 5 for acknowledgement of trademarks.Conclusion:Significant differences in efficiency, peak shape and selectivity are seen when analysing pyridine– a basic molecule.Increased tailing and retention, combined with reduced efficiency, are indicative of undesirablesecondary interactions between pyridine and silanol groups on the stationary phase surface.These interactions can also result in poor column reproducibility.ACE C18 columns have gained a well deserved reputation for providing excellent efficiency, peakshape and reproducibility. ACE SuperC18 columns further build upon this proven reputation toprovide the analyst with even better chromatographic performance across an extended range ofpH conditions under LC/MS compatible conditions.Si-OHACE ® StationaryPhases VirtuallyEliminate theNegative Effectsof Silanols on UHPLC& HPLC Separations3www.ace-hplc.comAustralia & New Zealand contact: infor@winlab.com.au or call +61 7 3205 5233