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Proceedings of the 5th International Symposium on EQUINE ...

Proceedings of the 5th International Symposium on EQUINE ...

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Equine Embryo Transferdisregarding degenerating oocytes) and as apercentage <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> total oocytes recovered.The fertilisati<strong>on</strong> rates for potentiallyfertilisable oocytes for <str<strong>on</strong>g>the</str<strong>on</strong>g> mM199, FF, and CHgroups (3, 3 and <strong>on</strong>e replicates, respectively) were11/15 (73%); 22/30 (73%) and 6/12 (50%),respectively. The fertilisati<strong>on</strong> rates based <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g>total oocytes recovered for each treatment were11/25 (44%), 22/37 (59%) and 6/16 (38%),respectively. The overall fertilisati<strong>on</strong> rate forpotentially fertilisable oocytes was 68% (39/57)and <str<strong>on</strong>g>the</str<strong>on</strong>g> fertilisati<strong>on</strong> rate for all oocytes recoveredwas 50% (39/78).One recovered oocyte was in Anaphase II witha dec<strong>on</strong>densing sperm head, and <strong>on</strong>e was inmitosis <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> first divisi<strong>on</strong>; <str<strong>on</strong>g>the</str<strong>on</strong>g>se were groupedwith <str<strong>on</strong>g>the</str<strong>on</strong>g> cells having 2 pr<strong>on</strong>uclei. Thus, <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>fertilised oocytes recovered, 20/39 (51%) had 2pr<strong>on</strong>uclei, 13/39 (33%) were 2-cell embryos, and6/39 (15%) were 3- to 4- cell embryos. There wasa significant difference in distributi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> embryostage am<strong>on</strong>g maturati<strong>on</strong> treatments. Theproporti<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g> fertilised oocytes that had cleavedto 2 or more - cell embryos in <str<strong>on</strong>g>the</str<strong>on</strong>g> mM199 and CHgroup were both significantly higher than that for<str<strong>on</strong>g>the</str<strong>on</strong>g> FF group (9/11, 82%; 5/6, 83%; and 6/22,27%, respectively; P

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