Proceedings of the 5th International Symposium on EQUINE ...

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Havemeyer Foundation Monograph Series No. 3CONTENTSEDITORS’ FOREWORD ....................................................................................................................Page viSESSION 1: HORSES AND HYBRIDSThe equids and embryos <strong>of</strong> J. Cossar Ewart: a glimpse <strong>of</strong> Victorian and Edwardian research inreproductionK. J. Betteridge ..................................................................................................................................Page 3Hysteroscopic uterotubal insemination <strong>of</strong> mares with low numbers <strong>of</strong> frozen-thawedejaculated and epididymal spermatozoaL. H. A. Morris, C. Tiplady, S. Wilsher and W. R. Allen ...................................................................Page 4Hysteroscopic insemination <strong>of</strong> non frozen and frozen unsexed and sexed equine sptermatozoaA. C. Lindsey, L. H. A. Morris, W. R. Allen, J. L. Schenk, J. K. Graham, J. E. Bruemmer andE. L. Squires .......................................................................................................................................Page 6Uterine blood flow during oestrous cycle and early pregnancy in maresH. Bollwein, R. Mayer and R. Stolla .................................................................................................Page 8SESSION 2: OOCYTES – MATURATION AND FERTILISATIONFollicular waves and selection <strong>of</strong> follicles in maresO. J. Gin<strong>the</strong>r, D. R. Bergfelt and F. X. Donadeu .............................................................................Page 15Organisation <strong>of</strong> <strong>the</strong> cytoskeleton during in vitro maturation <strong>of</strong> horse oocytesJ. L.Tremoleda, E. J. Schoevers, T. A. E. Stout, B. Colenbrander and M. M. Bevers .....................Page 19Effect <strong>of</strong> temperature and holding time on equine oocyte chromatin configurationH. G. Pedersen, E. E. Telfer and E. D. Watson ...............................................................................Page 22Effect <strong>of</strong> fetuin on <strong>the</strong> zona hardening and cortical ganules distribution in equineoocytes matured in vitroF. C. Landim-Alvarenga, S. E. A. Boyazoglu, G. E. Jr. Seidel and E. L. Squires ...........................Page 26Polyvinylalcohol is superior to bovine serum albumin in equine IVF mediumY. H. Choi, G. E. Seidel, Jr., S. Boyazoglu and E. L. Squires .........................................................Page 28The development <strong>of</strong> blastocysts after intracytoplasmic sperm injection <strong>of</strong> equine oocytesX. Li, L. H-A. Morris and W. R. Allen ............................................................................................Page 30Embryonic development <strong>of</strong> equine oocytes fertilised by ICSIC. Galli, G. Crotti, G. Mari and G. Lazzari ...................................................................................Page 32Effect <strong>of</strong> invasive adenylate cyclase during oocyte maturation on development <strong>of</strong>equine embryos following ICSIL. J. Maclellan, M. M. Sims and E. L Squires ................................................................................Page 35iii
Equine Embryo TransferChromatin configuration, meiotic competence and success <strong>of</strong> intra-cytoplasmic sperminjection in horse oocytes collected by follicular aspiration or scrapingM. E. Dell’Aquila, M. Masterson, F. Maritato and K. Hinrichs ....................................................Page 37Transvaginal intrafollicular sperm cell injection in <strong>the</strong> cyclic mareM. Meinjtes, B. Eilts, R. Cochran, K. Graff, R. Denniston, D. Paccamonti and R. Godke ...........Page 39Fertilisation rates <strong>of</strong> in vitro matured oocytes transferred into <strong>the</strong> oviduct <strong>of</strong> inseminated maresC. C. Love, S. P. Brinsko, D. D. Varner and K. Hinrichs ...............................................................Page 41Comparison <strong>of</strong> bovine and equine oocytes as host cytoplasts for equine nuclear transferK. Hinrichs, T. Shin, C. C. Love, D. D. Varner and M. E. Westhusin ............................................Page 43Nuclear transfer embryos in <strong>the</strong> horseB. C. Reggio, M. Sansinena, R. A. Cochran, A. Guitreau, J. A. Carter, R. S. Dennistonand R. A. Godke ..............................................................................................................................Page 45Effects <strong>of</strong> oocyte maturity and methods <strong>of</strong> collection, culture and insemination onequine oocyte transferE. M. Carnevale, L. J. Maclellan, T. J. Scott, M. A. Coutinho da Silva, C. F. Scogginand E. L. Squires .............................................................................................................................Page 47SESSION 3: EMBRYO COOLING AND FREEZINGNovel methods <strong>of</strong> embryo cryopreservationS. P. Leibo ......................................................................................................................................Page 51Comparison <strong>of</strong> glycerol and ethylene glycol in equine embryo freexing using confocalmicroscopy, dapi-staining and nonsurgical transferM. Huhtinen, A. Sjöholm and J. Paranko .......................................................................................Page 52Cryopreservation <strong>of</strong> equine embryos by OPS, cryoloop, or conventional slow cooling methodsN. Oberstein, M. K. O’Donovan, J. E. Bruemmer, M. Lane, G. E. Seidel, Jr.,E. M. Carnevale and E. L. Squires .................................................................................................Page 54Metabolic activity <strong>of</strong> equine blastocysts cryopreserved in ethylene glycol and galactoseM. K. O’Donovan, N. Oberstein, J. E. Bruemmer, M. Lane, G. E. Seidel, Jr.,D. K. Gardner and E. L. Squires ...................................................................................................Page 57Production <strong>of</strong> capsular material by equine trophoblast transplanted into immunodeficient miceA. Albihn, J. Samper, J. G. Oriol, B. A. Croy and K. J. Betteridge ................................................Page 60Does <strong>the</strong> embryonic capsule impede <strong>the</strong> freezing <strong>of</strong> equine embryos?E. Legrand, J. M. Krawiecki, D. Tainturier, P. Cornière, H. Delajarraud andJ. F. Bruyas .....................................................................................................................................Page 62When do equine embryos enter <strong>the</strong> uterine cavity: an attempt to answer?I. Battut, A. Grandchamp des Raux, J. L. Nicaise, F. Fieni, D. Tainturier and J.F. Bruyas ..........Page 66SESSION 4: COMMERCIALEMBRYO TRANSFERChanges <strong>of</strong> PGFM, progesterone and LH secretion patterns in relation to cycle lengthafter cervical manipulation in maresJ. Handler, M. Königsh<strong>of</strong>er, H. Kindahl, H.-O. Hoppen, J. E. Aurich and B. Aurich ..................Page 71The influences <strong>of</strong> maternal size, age and parity on placental and fetal developmentin <strong>the</strong> horseS. Wilsher and W. R. Allen .............................................................................................................Page 74iv
Page 1 and 2: HavemeyerFoundationHavemeyer Founda
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Equine Embryo TransferLANDIM-ALVARE
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