Proceedings of the 5th International Symposium on EQUINE ...
Proceedings of the 5th International Symposium on EQUINE ...
Proceedings of the 5th International Symposium on EQUINE ...
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Havemeyer Foundati<strong>on</strong> M<strong>on</strong>ograph Series No. 323/43 (53%) than BSA, 5/46 (11%). Six <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> 23oocytes were polyspermic in <str<strong>on</strong>g>the</str<strong>on</strong>g> PVA group vs 1<str<strong>on</strong>g>of</str<strong>on</strong>g> 5 oocytes in <str<strong>on</strong>g>the</str<strong>on</strong>g> BSA group.CONCLUSIONSStalli<strong>on</strong> sperm penetrati<strong>on</strong> rates <str<strong>on</strong>g>of</str<strong>on</strong>g> bovine z<strong>on</strong>afreeoocytes and partial z<strong>on</strong>a-removed equineoocytes were superior with PVA compared to BSAsupplementati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> capacitati<strong>on</strong> and fertilisati<strong>on</strong>media. The reas<strong>on</strong>/mechanism for PVA superiorityto BSA for capacitati<strong>on</strong> and fertilisati<strong>on</strong> by stalli<strong>on</strong>sperm in <str<strong>on</strong>g>the</str<strong>on</strong>g> system used requires fur<str<strong>on</strong>g>the</str<strong>on</strong>g>rexperimentati<strong>on</strong>. However, <str<strong>on</strong>g>the</str<strong>on</strong>g> chemically definedmedium used in this study will simplifyinvestigati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> detailed mechanisms <str<strong>on</strong>g>of</str<strong>on</strong>g>capacitati<strong>on</strong>. Fur<str<strong>on</strong>g>the</str<strong>on</strong>g>r, bovine z<strong>on</strong>a-free oocyteswere useful for assaying in vitro capacitati<strong>on</strong> andfertilisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> stalli<strong>on</strong> sperm.REFERENCESBavister, B.D. (1995) Culture <str<strong>on</strong>g>of</str<strong>on</strong>g> preimplantati<strong>on</strong>embryos: facts and artifacts. Hum. Reprod. Update.1, 91-148.Choi, Y.H, Okada ,Y., Hochi, S., Braun, J., Sato, K. andOguri, N. (1994) In vitro fertilisati<strong>on</strong> rate <str<strong>on</strong>g>of</str<strong>on</strong>g> horseoocytes with partially removed z<strong>on</strong>ae. Theriogenol.42, 795-802.29