FINAL PROGR AM - American Society of Gene & Cell Therapy
FINAL PROGR AM - American Society of Gene & Cell Therapy
FINAL PROGR AM - American Society of Gene & Cell Therapy
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<strong>AM</strong>ERICAN SOCIETY OF GENE THERAPY • Final Program 57<br />
ABSTRACTS<br />
THURSDAY, JUNE 2<br />
177<br />
Analysis <strong>of</strong> the High Shear <strong>Cell</strong> Lysis Device and the Air-<br />
Mixed Neutralization Column Used in the Recovery <strong>of</strong><br />
Plasmid DNA from E. coli <strong>Cell</strong>s<br />
Sriram Ramakrishnan, Hugo Gonzalez, Henry L. Hebel<br />
178<br />
A Novel RNA Quantitation Method Using Hydrophobic<br />
Interaction Chromatography-High Performance Liquid<br />
Chromatography<br />
Shane Johnson, Andrew Doig, Henry Hebel, Hugo Gonzalez,<br />
Heather Attra<br />
179<br />
Transgenesis <strong>of</strong> B Lymphocytes by Bacterial DNA Is<br />
TLR9 Independent<br />
Mara Gerloni, Matthew Wheeler, Xocthil Cortez-Gonzales,<br />
Ilenia Pellicciotta, Maurizio Zanetti<br />
180<br />
pDNAVACC Vector Family: High Throughput Intracellular<br />
Targeting DNA Vaccine Plasmids Created by <strong>Gene</strong><br />
Self Assembly<br />
James A. Williams, Jeremy Luke, Lance Johnson, Clague P.<br />
Hodgson<br />
181<br />
The Effect <strong>of</strong> Dissolved Oxygen Fluctuations on <strong>Cell</strong><br />
Growth and Plasmid Yield<br />
Sriram Ramakrishnan, Justin Miller, Jeffrey Darnell, Henry L.<br />
Hebel<br />
182<br />
An Effective and Valuable Process Using Disposable<br />
Membrane-Based Technologies for Pure Plasmid DNA<br />
Isolation and Purification: Throughput, Quality, and<br />
Cleaning Validation Advantages<br />
Hugo L. Gonzalez, Sriram Ramakrishnan, Shane Johnson,<br />
Andrew Doig, Henry L. Hebel<br />
183<br />
Development <strong>of</strong> Ex-Vivo <strong>Gene</strong> <strong>Therapy</strong> Procedures for<br />
Central Nervous System Diseases, Based on Homologous<br />
Recombination in Human Neural Stem <strong>Cell</strong>s<br />
Carlos A. Bueno Lopez, Alberto Martinez Serrano<br />
184<br />
Nonvirally Electrotransfected Human Mesenchymal<br />
Stem <strong>Cell</strong>s: A Novel Platform for Bone <strong>Gene</strong> <strong>Therapy</strong><br />
Based on Autocrine and Paracrine Mechanisms<br />
Hadi Aslan, Yoram Zilberman, Jin Zhong Li, Gregory A.<br />
Helm, Zulma Gazit, Dan Gazit<br />
185<br />
Poly-lactide-co-glycolide Acid (PLGA) Nanoparticles for<br />
DNA Vaccination<br />
Jacob Gvili, Marcelle Machluf<br />
186<br />
Secreted Extracellular Vesicles <strong>of</strong> Baculovirus GP64<br />
Interact with Plasmid DNA to Enhance Transfection<br />
Ghiabe H. Guibinga, Andrew C. Hinton, Atsushi Miyanohara,<br />
Theodore Friedmann<br />
187<br />
Strategies <strong>of</strong> Intravascular Delivery <strong>of</strong> Naked DNA into<br />
Rat Skeletal Muscle<br />
Zhen Jia, István Dankó<br />
188<br />
Transfer <strong>of</strong> DNA in Tissues Using Local Fast Injection<br />
Franck M. Andre, Christel O. Cournil-Henrionnet, Paule<br />
Opolon, Lluis M. Mir<br />
189<br />
Improvement <strong>of</strong> In-Vivo Transfer <strong>of</strong> Plasmid DNA in<br />
Muscle: Comparison <strong>of</strong> Electroporation Versus Ultrasound<br />
Geke A. Hospers, Wendy A. Dam, Marc H. DeBaets, Mario H.<br />
Losen, Coby Meijer, Nanno H. Mulder<br />
190<br />
Improvement <strong>of</strong> Electroporation Devices and Methods<br />
for Use with Plasmid-Based Therapeutics and DNA<br />
Vaccine Applications in Small and Large Animals<br />
Amir S. Khan, Ruxandra Draghia-Akli<br />
191<br />
Identification <strong>of</strong> a Novel Functional Domain in the<br />
Sleeping Beauty Transposase: Towards Alleviating the<br />
Restriction <strong>of</strong> SB Overproduction Inhibition<br />
Julie Park, Stephen R. Yant, Mark A. Kay<br />
192<br />
Non-Viral, Long-Term <strong>Gene</strong> Expression in Hematopoietic<br />
<strong>Cell</strong>s Using the Sleeping Beauty Transposon System<br />
Linda N. Liu, Jeffrey J. Essner, Rama Shivakumar, Linhong Li,<br />
Shannon A. Wadman, Karl J. Clark, Aidas Nasevicious, Joseph<br />
C. Fratantoni, R. Scott McIvor, Perry B. Hackett<br />
193<br />
Direct Comparison <strong>of</strong> Integration Efficiency and Stable<br />
<strong>Gene</strong> Expression Mediated by the Sleeping Beauty<br />
Transposon System and by the PhiC31 Phage Integrase<br />
System in Cultured Human Fibroblasts and in Various<br />
Stem <strong>Cell</strong> Targets<br />
Andy Wilber, Fernando Montoya, Aron M. Geurts, David A.<br />
Largaespada, Uma Lakshmipathy, R. Scott McIvor<br />
194<br />
Characteristics <strong>of</strong> Site-Specific Integration as Mediated<br />
by PhiC31 Integrase in the Human Genome<br />
Joylette L. Portlock, Thomas W. Chalberg, Eric C. Olivares,<br />
Bhaskar Thyagarajan, Patrick J. Kirby, Michele P. Calos
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