DEACTIVATED CAPILLARY TUBING - Cromlab

METHYL AND PHENYLMETHYL DEACTIVATED TUBINGPERFORMANCECRITERIADeactivated tubing is an essentialingredient in maintaining the highestperformance of your chromatographicsystem. Being multi-functional,the quality of this tubing needs to beequal to that of our analytical column.Tubing used as retention gaps/guardcolumns need to achieve the highestpossible level of chemical inertnessand thermal stability, ensuring nointerference with the quantitative andqualitative processes of an analysis.In addition to the quality of the surfacedeactivation, deactivated tubing usedas retention gaps/guard columns needto fulfil certain other criteria:¥ Wettability by Solvent of Interest¥ Minimal Retention or Interaction ofSolute¥ No Retention of SolventGUARANTEEDPERFORMANCETo guarantee the quality of the methyland phenylmethyl deactivated surfacesin tubing used as retention gaps/guardcolumns (0.22mm, 0.32mm and0.53mm I.D.), SGE individually teststhe performance of each 25 metrelength from which all shorter(2 and 5 metre) lengths are prepared.This test report is included with eachcolumn providing a history of it'sperformance.Methyl and phenylmethyl deactivatedtubing of diameters 0.22mm-0.53mmI.D. are prepared by a uniqueprocedure enabling operatingtemperatures up to 400¡C.METHYL DEACTIVATED CAPILLARY TUBINGID OD Length Part No. Description(mm) (mm) (m)0.025 0.285 2 062442 2VSD/0250.050 0.230 2 062444 2VSD/0500.060 0.230 2 0662445 2VSD/0600.075 0.230 2 0624450 2VSD/0750.100 0.300 25 0624455 25VSD/1000.110 0.170 2 062446 2VSD/1100.150 0.230 2 0624460 2VSD/1500.150 0.23 5 0624461 5VSD/1500.150 0.325 2 0624465 2VSD/1500.170 0.300 5 062449 5VSD/1700.220 0.325 2 0624469 2VSMD/2200.220 0.325 5 062447 5VSMD/2200.220 0.325 25 0624474 25VSMD/2200.320 0.43 2 0624470 2VSMD/3200.320 0.43 5 0624471 5VSMD/3200.320 0.43 25 0624473 25VSMD/3200.530 0.68 2 0624479 2VSMD/5300.530 0.68 5 062448 5VSMD/5300.530 0.68 25 064034 25VSMD/530ECONOMICAL FIVE-PACKS- METHYL DEACTIVATED CAPILLARY TUBINGID OD Length Part No. Description(mm) (mm) (m)0.22 0.325 5 x 5 064050 FK-5VSMD/2200.32 0.43 5 x 5 064052 FK-5VSMD/3200.53 0.68 5 x 5 064054 FK-5VSMD/530TRANSFER LINESSGE also offers an extensive rangeof small internal diameter(0.025mm-0.17mm) methyldeactivated tubing ideal for use astransfer lines in GC/MS and multidetectorsystems.Manufactured to the highest standards,this tubing can be used to re-directeffluent flow to two or more detectorssystems without any sacrifice toresolution or absorption of compounds.Used in mass spectrometers, small I.D.deactivated tubing can simplify columnchange-over procedures. Restrictingthe flow into the mass spectrometer byusing short length of small I.D. tubingcan allow the analytical column to bechanged without turning the vacuumdown.Larger bore deactivated tubing(0.22mm-0.53mm) also provideschemically inert flow paths for sampleintroduction systems.¥ Purge & Trap Systems¥ Headspace Analysers¥ Multidemensional Systems

PHENYLMETHYL DEACTIVATED CAPILLARY TUBINGID OD Length Part No. Description(mm) (mm) (m)0.22 0.325 2 064100 2VSPD/2200.22 0.325 5 064102 5VSPD/2200.22 0.325 25 064104 25VSPD/2200.32 0.43 2 064106 2VSPD/3200.32 0.43 5 064108 5VSPD/3200.32 0.43 25 064110 25VSPD/3200.53 0.68 2 064112 2VSPD/5300.53 0.68 5 064114 5VSPD/5300.53 0.68 25 064116 25VSPD/530ECONOMICAL FIVE-PACKS- PHENYLMETHYL DEACTIVATED CAPILLARY TUBINGID OD Length Part No. Description(mm) (mm) (m)0.22 0.325 5 x 5 064150 FK-5VSPD/2200.32 0.43 5 x 5 064152 FK-5VSPD/3200.53 0.68 5 x 5 064154 FK-5VSPD/530PHENYLMETHYLDEACTIVATIONIn addition to the widely used nonpolarmethyl surface deactivatedtubing, SGE now introduces a newmedium polarity range ofphenylmethyl deactivated tubing.This range has been developed toprovide an additional choice of surfacedeactivation, ensuring goodchromatography is obtained for thecomplete range of solvents used insample preparation. The additionalsurface polarity improves the wettingor wettability of the quartz surface forchlorinated and some aromaticsolvents, therefore ensuring good peakshapes are obtained for both early andlate eluting compounds.2 METRE 0.32MM I.D. PHENYLMETHYL DEACTIVATEDGUARD COLUMNGuard Column:Part No. 0641062VSPD/320Analytical ColumnPhase:BPX5, 0.5µm filmColumn:12m x 0.32mm I.D.Initial Temp.:80°C, 0.5 minRate:10°C/minFinal Temp.: 280°CCarrier Gas:He, 4 psiInjection Mode: Split, 1µlDetector:F.I.D.1 234 5Solvent: Dichloromethane1. Decane2. Naphthalene3. Undecanol4. 4-Nitrophenol5. 4-Nitroaniline6. Phenanthrene7. Anthracene8. Eicosane768

CARBOWAX DEACTIVATEDSGE now offers a range of carbowaxdeactivated tubing suitable for use asguard columns for direct aqueousinjection. The surface deactivation iswater resistant and, therefore, suitablefor both vapourising and coolon-column injections of solutionscontaining substantial quantities ofwater.Column deterioration is oftenattributed to the introduction of nonvolatile("dirty") components, whereas,in reality, the loss in performance canbe caused by the interaction of waterwith the quartz surface.Samples from LC analyses can beanalysed by GC without any furtherpreparation. Reverse-phase HPLC isdifficult to interface directly withcapillary gas chromatography as nearlyall samples contain mixtures of waterwith either methanol, acetonitrile ortetrahyrofuran. The aggressive natureof water is such that only a fewinjections are needed to produce anactive guard column which can thenselectively absorb compounds from asample.Large aqueous injections are nowpossible when a short length ofcarbowax deactivated tubing is used,enabling the detection of compoundspresent in water at only low ppmlevels. The polar nature of thecarbowax tubing allowsnon-functionalised compounds liken-paraffins and simple aromaticsdissolved in aqueous solutions to beanalysed directly, without any furthersample preparation.CARBOWAX DEACTIVATED TUBINGGuard Column:Part No. 064204Phase:Column:Initial Temp.:Rate:Final Temp.:Carrier Gas:1 235VSCD/530BP20, 1.0µm film25m x 0.32mm I.D.90°C, 4 min8°C/min220°C, 5 minHe, 15 psiInjection Mode: Cool on-columnInjection Volume: 6µlSample Concentration: 2-6ppm each componentSolvent:60/40 water/methanolChromatogram of the 20th consecutive injection.4CARBOWAX DEACTIVATED CAPILLARY TUBING567891. m-Xylene2. p-Xylene3. o-Xylene4. Pentadecane5. Octanol6. Octadecane7. Dimethylaniline8. Dimethylphenol9. BiphenylID OD Length Part No. Description(mm) (mm) (m)0.32 0.325 5 064200 5VSCD/3200.32 0.43 25 064202 25VSCD/3200.53 0.68 5 064204 5VSCD/5300.53 0.68 25 064206 25VSCD/530ECONOMICAL FIVE-PACKS- CARBOWAX DEACTIVATED CAPILLARY TUBING0.32 0.43 5 x 5 064220 FK-5VSCD/3200.53 0.73 5 x 5 064222 FK-5VSCD/530

COLUMN CONNECTIONSMINI UNIONS¥ Glass lined bore - GLTÔ¥ Low dead volumeThese unions are specifically designedfor connecting flexible silica columns andtubing. The glass lined bore ensures aninert connection and precise insidediameter. Two columns can beconnected or a broken column joinedwith negligible loss in column efficiencyor inertness.A kit contains a special GLT lined unionand all ferrules required for installation.Column: 25QC2/BP1-1.01. Decane 4. UndecanolTemp.: 180°C2. Chlorophenol 5. BiphenylInjection: Split, 0.1µl3. n-Decylamine 6. PentadecaneCarrier Gas: 10 psi, H 2Neff. 77,6005 6Neff. 76,50015 644123Without UnionCOLUMN CONNECTIONS23With UnionPart No. Code Union I.D. Capillary Column I.D.103430 MVSU/003 0.3mm 0.1mm103431 MVSU/004 0.4mm 0.22mm103432 MVSU/005 0.5mm 0.32mmMVSU103433 MVSU/008 0.8mm 0.53mmINLET SPLITTERS¥ For columns from 0.22 to 0.53mm I.D.¥ Ideal for confirmation retention data¥ Small internal volumes¥ Inert flow path GLT and fused silicaWhere an injected sample is to be splitbetween two capillary columns forsimultaneous analyses, the VSIS isrecommended.The low internal volume, glass lineddesign, ensures an inert low dead volumeconnection.The two capillary columns used (usuallydiffering in polarity) must have the samelength and inside diameter to obtain equalsample splitting.Three kits are available to suit 0.22, 0.32and 0.53mm I.D. capillary columns. Thekits consist of a special GLT lined unionand all ferrules required for installation.VSIS - 5InjectorRetention Gap/Guard ColumnVSIS - 2&3Column 1 Column 2COLUMN CONNECTIONSPart No. Code Description123632 VSIS-2 For 0.22mm I.D. Capillary Columns123633 VSIS-3 For 0.32mm I.D. Capillary Columns123634 VSIS-5 For 0.53mm I.D. Capillary ColumnsWeb Site: www.sge.comFused silica capillary columns are manufactured under license granted by Hewlett Packard Company.Specifications are subject to change without notice.GLTÔ is a Trademark of SGE International Pty. Ltd.SGE International Pty Ltd SGE Australia Pty Ltd SGE, Incorporated (USA) SGE Europe Ltd SGE Japan Inc.Tel: (+613) 9837 4200 Toll Free: 1800 800 167 Toll Free: (800) 945 6154 Tel: +44 (0) 1908 568844 Tel: (045) 263 1571Fax:(+613) 9874 5672 Fax: (03) 9872 5103 Fax: (512) 836 9159 Fax: +44 (0) 1908 566790 Fax: (045) 263 1565e-mail: support@sge.com.au e-mail: sales@sge.com.au e-mail: usa@sge.com.au e-mail: uk@sge.com.au e-mail: sgejpn@itjit.ne.jpSGE (France) Sarl SGE Italia Srl SGE Deutschland GmbH SGE China Service Centre SGE IndiaTel: (01) 4382 2943 Tel: (06) 4429 0206 Tel: (06150) 2078 Tel: 86 (10) 8656 1666 Tel: (022) 411 5896Fax: (01) 4382 4268 Fax: (06) 4429 0724 Fax: (06150) 14768 Fax: 86 (010) 6587 2917 Fax: (022) 415 6592e-mail: france@sge.com.au e-mail: sge.italia@tin.it e-mail: germany@sge.com.au e-mail: sge@bj.col.com.cn e-mail: sgeindia@bom4.vsnl.net.inPublication No. PD-0002-C Rev:01 12/99