ACCLAIM oa - Cromlab

Ch e m i c a l ApplicationsSurfactantsSeparation of Different Polyethylene GlycolsSeparation of Quaternary Imidazolinium CompoundsmVPEG-300PEG-400Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) H 2 OGradient: 3–15% A in 20 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSDmV2-Hydroxyethylbenzylcoco imidazoliniumchlorideDiethyl heptadecylimidazoliniumethylsulfateColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–85% A in 30 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSDPEG-6000 5 10 15 20Minutes0 5 10 15 20 25 30Minutes2107721117µS01Separation of Cationic Surfactants withSuppressed Conductivity Detection25345Minutes10Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 20 mM acetic acidGradient: 20–50% A in 15 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: Suppressed CD (AMMS III)Peaks: 1. Lauryl pyridinium chloride2. Lauryldimethylbenzylammonium chloride3. Octylphenoxyethoxyethyldimethylbenzyl ammonium chloride4. Cetyltrimethylammonium chloride5. Cetylpyridinium chloride1521086mVAnalysis of PEG-200 Distearate0 5 10 15 20MinutesOOColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) CH 3 CN,(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–85% A in 10 min,then hold 85% A for 10 minTemperature: 30 °CFlow Rate: 1.5 mL/minInj. Volume: 20 µLDetection: ELS detectorSample: PEG-200 DistearateOOOO22796POE(30) Ammonium Lauryl SulfateDyes15µSColumn: Acclaim PA2, 5 µmDimension: 4.6 × 150 mmMobile phase: Acetonitrile/25 mM borate buffer v/v 40/60Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 20 µL (1000 ppm)Detection: Suppressed conductivity detection(AMMS III)Sample: POE(30) Ammonium lauryl sulfate40mAUSudan Dyes on Acclaim 120 C18132 4Column: Acclaim 120 C18, 3 µmDimensions: 4.6 × 150 mmMobile Phase: 50/50/0.5 MeOH/acetonitrile/HOAcFlow Rate: 1.0 mL/minInj. Volume: 20 µLDetection: UV, 280 nmPeaks: 1. Sudan I2. Sudan I, impurity3. Sudan II4. Sudan III00 10 2025Minutes211370 1 2 3 4 5 6Minutes20411APPLICATIONSwww.dionex.com - 45 -