ACCLAIM oa - Cromlab

Ch e m i c a l ApplicationsSurfactantsAnalysis of Sodium Lauryl Sulfate Using LC-ESI-MSAnalysis of PEG Monoethyl Ether (MW-550)AbundanceColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: Acetonitrile/0.1 M NH 4 OAc,pH 5.4 v/v 75/25Temperature: 25 °CFlow Rate: 1 mL/min(0.2 mL/min to mass spectrometer)Inj. Volume: 25 µLDetection: MS (ESI negative, probe 300 °C,cone 20 V needle 2.5 KV,SIM 265.2, span 0.3 m/z)Peak: Sodium lauryl sulfate (1 ppm)mVColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) H 2 OGradient: 5–20% A in 25 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSD0 5 10 15 20Minutes211260 2 4 6 810 12 14 16 18 20 22 25Minutes21118AbundanceAnalysis of Triton X-100 Using LC-ESI-MS0 4 8 12 16 20 24MinutesColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: Acetonitrile/50 mMNH 4 OAc, pH 5.4 v/v 45/55Temperature: 25 °CFlow Rate: 1 mL/min (0.2 mL/minto mass spectrometer)Inj. Volume: 25 µLDetection: MS (ESI positive, probe 300 °C,cone 20 V, needle 2.5 kV, SIM 312.2,356.2, 400.4, 444.4, 488.4, 532.4,576.5, 620.6, 664.6, 708.6, 752.5,796.6, 840.7, 884.7, 928.7, 972.7,1016.8, 1060.7, 1104.7, span 0.5 m/z)Peak: Triton X-100 (100 ppm)C 8 H 17(OC 2 HCH 2 ) n OHmVSeparation of Hydrotrope, Anionic, Cationic,and Amphoteric SurfactantsColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) CH 3 CN,(B) 0.16 M NH 4 OAc, pH 5.4Gradient: 25% to 85% A in 30 min,then hold 85% A for 10 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 25 µL10Detection: ELS detector12 3 45 6 789111213Peaks:1. Xylene sulfonate2. Laurylpyridinium chloride3. Lauryldimethylbenzyl ammonium chloride4. Octylphenoxyethoxyethyl dimethylbenzylammonium chloride5. Myristamidopropyl betaine6. Cetyltrimethyl ammonium chloride7. Cetylpyridinium chloride8. Cetyl betaine9. Decyl sulfate10. Dodecyl sulfate11. C 10 -LAS12. C 11 -LAS13. C 12 -LAS14. C 13 -LAS14n ~ 10Triton X-1000 5 10 15 20 25 30 35Minutes2112822794UV, 225 nmDodecylbenzene sulfonateELSDAnalysis of a Laundry Washing DetergentDodecylbenzene sulfonateand Laureth sulfateColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–80% A in 30 min,then hold at 80% A for 15 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 5 µLDetection: ELSD, UVSample Prep: Dilute 10× with 70% acetonitrile,then filtered through 0.2-µmmembraneUV, 225 nmDodecylbenzenesulfonateELSDDodecylbenzene sulfonateand laureth sulfateAnalysis of a Dishwashing LiquidColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–80% A in 30 min,then hold at 80% A for 10 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSD, UVSample Prep: Dilute 10× with 70% acetonitrile,then filtered through 0.2-µmmembraneAPPLICATIONS0 5 10 15 20 25 30 35 40 45Minutes0 5 10 15 20 25 30 35 40Minutes2108421085www.dionex.com - 43 -