11.07.2015 Views

ACCLAIM oa - Cromlab

ACCLAIM oa - Cromlab

ACCLAIM oa - Cromlab

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

Environmental ApplicationsEnvironmental50Brodifacoum in Rodent BaitColumn: Acclaim 120 C18, 5 µm,4.6 × 150 mmPump: Summit P580 HPG/4Mobile Phase: (A) Acetonitrile(B) 0.10% conc. phosphoric acid in watermAU1Isocratic A:B 82.5:17.52Flow: 1.20 mL/minTemperature: 30 °C0Injection: Summit ASI-100, 10 µLDetector:0 Minutes10Summit UVD 340U diode array,265 nm, and spectra 200–400 nmSample Prep.:- Mutually saturate ethyl acetate and waterfor use in the next step- Shake 1.0g of finely ground bait with 10.0 mLethyl acetate, 5.0 mL water and 0.10 mL acetic acid- Centrifuge to separate the emulsion- Evaporate 3.0 mL of ethyl acetate fraction to dryness- Reconstitute in 0.60 mL acetonitrile- FilterPeaks: 1. Brodifacoum isomer 12. Brodifacoum isomer 2215220.10AU012Separation of Phthalate Esters inEPA Method 606 Using Acclaim PA3 45 60 5 10 15MinutesColumn: Acclaim PA, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) D.I. H 2 O(B) MeOHGradient: Time (min) % B0 2510 10015 100Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: UV, 254 nmPeaks: (100 µg/mL each)1. Dimethyl phthalate2. Diethyl phthalate3. Bis(2-ethylhexyl) phthalate4. Di-n-butyl phthalate5. Di-n-octyl phthalate6. Butyl benzyl phthalate20107LC Separation of Nitrosamines in EPA 8270 Using Acclaim PAAU12 3 4 5 60 10 20 30Minutes78 9Column: Acclaim PA, 5 µmDimensions: 4.6 × 250 mmMobile Phase: (A) D.I. H 2 O(B) AcetonitrileGradient: Time (min) % B0 15 125 9030 90Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 15 µLDetection: UV, 230 nmPeaks: (20 µg/mL)1. N-Nitroso-dimethylamine2. N-Nitroso-morpholine3. N-Nitroso-methylethylamine4. N-Nitroso-pyrrolidine5. N-Nitroso-diethylamine6. N-Nitroso-piperidine7. N-Nitroso-dipropylamine8. N-Nitroso-dibutylamine9. N-Nitroso-diphenylamineNote: This gradient will also resolve benzidines.20109a0.45AU0Phenol Standards by EPA Method 604 on Acclaim PA13967102 4 5 80 5 10 15 20MinutesNote: The lower pH provided by methanesulfonic acid(compared to acetic acid in the standard method) givesoptimum separation for the Acclaim PA column.11Column: Acclaim PA, 3 µmDimensions: 4.6 × 150 mmMobile Phase: (A) 2.5 mM MSA in H 2 O(B) MethanolGradient: Time (min) % B0 604.5 6010.5 9520 95Temperature: 25 °CFlow Rate: 1 mL/minInj. Volume: 5 µLDetection: UV, 285 nmPeaks:(50– 250 µg/mL each)1. Phenol2. 2-Chlorophenol3. 4-Nitrophenol4. 2-Nitrophenol5. 2,4-Dimethylphenol6. 4-Chloro-3-methylphenol7. 2,4-Dichlorophenol8. 2,4-Dinitrophenol9. 2-Methyl-4,6-dichlorophenol10. 2,4,6-Trichlorophenol11. Pentachlorophenol20106APPLICATIONS054321LC/MS Detection of Herbicides on Acclaim 120 C18Column: Acclaim 120 C18, 3 µmDimensions: 2.1 × 100 mm0.0010Mobile Phase: 70/30% methanol/10.1% formic acidFlow Rate: 0.25 mL/minDetection: AQA MS, +ESI, 30 V, 200 °C,SIM as indicatedµA2Peaks: m/z mass injected1. Monuron 199 1 ng32. Atrazine 216.1 13. Propazine 230 24. Diuron 233 2.515. Linuron 249 2–0.00012 3 4 5 0 2.5 5.0MinutesMinutes7.5 10.017922Benzidines by EPA Method 605 on Acclaim 120 C18with Electrochemical DetectionColumn: Acclaim 120 C18, 3 µmDimensions: 2.1 × 100 mmMobile Phase: 50/50 acetonitrile/100 mM NaOAc, pH 4.7Flow Rate: 0.17 mL/minInj. Volume: 1 µLDetection: Glassy carbon electrode, 800 mV(1 µg/mL)Peaks 1. Benzidine2. 3.3'-Dimethylbenzidine3. 3.3'-DichlorobenzidineNote: Scaling down the column size and flowrate produces a superproportional increase insensitivity in the electrochemical detector.20400- 34 -www.dionex.com

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!