ACCLAIM oa - Cromlab

Acclaim OAChapter 6: Acclaim OA forOrganic AcidsOptimized and application-tested for the analysis ofhydrophilic organic acidsAcclaim OA organic acid columns are special reversed-phasesilica columns, designed for the separation of aliphatic and aromaticorganic acids at low pH with UV-Visible detection. TheAcclaim OA uses a patented polar-embedded stationary phasethat allows a broad range of operating conditions, including 100%aqueous mobile phases.The Acclaim OA is the recommended column for determiningsmall hydrophilic organic acids, C1 to C7 aliphatic acids, andhydrophilic aromatic acid and is also valuable for the analysis andquality assurance of food and beverage products, pharmaceuticalpreparations, plating baths for semiconductor manufacturing,manufacturing chemicals, and chemical intermediates.The Acclaim OA columns are packed in metal-free PEEK columnbodies, to eliminate unwanted interactions between the analytesand the column body. Benefits of the Acclaim OA columnsinclude:• Use-tested to guarantee consistent organic acidseparations• Compatible with 100% aqueous mobile phases, optimumfor reversed-phase retention of hydrophilic aliphaticorganic acids• Hydrolytic stability at low-pH conditions, optimumfor reversed-phase retention of organic acids• Ideal selectivity for separating a wide spectrum oforganic acids, including hydrophilic aliphatic acids,C1 to C7 aliphatic acids, aromatic acids, and selectedamino acids• Excellent peak shapes for organic acids• High reversed-phase capacity with retention similar tostandard C18 phases• Suitable for analysis of foods, beverages, pharmaceuticals,chemical intermediates, and environmentalsamples• Ideal for quality assurance offering fast run times andminimal sample preparationOARecommended Ranges of OperationpH Range 2.0–8.0Pressure limit 4500 psi (2000 psi above 50%organic solvent content)Organic solventsHydrophilic Organic AcidsHO OHO OO OHOO OHOHOOH OHOHOO OHH OHHOOOHOHO1. Oxalic acid 2. Tartaric acid 3. Formc acid 4. Malic acid 5. iso-Citric acidOHOOO OHOOOHOH OH OHH OHOHO3 CH 3 COHHOOOOHO6. Lactic acid 7. Acetic acid 8. Citric acid 9. Succinic acid 10. Fumaric acidHOOO O O OOH OHOH OH11. cis-Acontic acid 12. trans-Aconitic acidOOH0–100% acetonitrile or methanol19511Surface chemistryUSP codeEndcappingPore sizePhysical SpecificationsProprietarynaYes120–140 ÅSurface area 290–320 m 2 /gParticle diameter 4.2–4.5 µmGuaranteed Performance for Organic AcidsThe performance of the Acclaim OA columns is guaranteedfor the separation of aliphatic and aromatic organic acids at lowpH. These columns undergo extensive testing to ensure columnto-columnreproducibility, and are shipped with quality assurancereports detailing these tests. The Acclaim OA columns are usetestedfor two specific applications—isocratic and gradient.These operating conditions provide a good starting point formethods development.Performance SpecificationsLot Metal activity ratio 0.80–1.50Gradient organic acids Baseline drift