Novel genetic and epigenetic alterations in ... - Ous-research.no
Novel genetic and epigenetic alterations in ... - Ous-research.no
Novel genetic and epigenetic alterations in ... - Ous-research.no
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Previous studies have shown that RCC2 (also k<strong>no</strong>wn as TD-60) is associated with thesegregation of chromosomes <strong>in</strong> metaphase.[34] Indeed, RCC2 is critical for the <strong>in</strong>tegratio<strong>no</strong>f k<strong>in</strong>etochores <strong>in</strong>to the mitotic sp<strong>in</strong>dle, <strong>and</strong> may be required for overall sp<strong>in</strong>dleassembly.[35] siRNA suppression shows that RCC2 is absolutely required for progressionfrom prometaphase to metaphase <strong>and</strong> that its suppression activates the sp<strong>in</strong>dle assemblycheckpo<strong>in</strong>t, hence creat<strong>in</strong>g an effective G2/M arrest, <strong>in</strong>def<strong>in</strong>itely block<strong>in</strong>g cells fromcomplet<strong>in</strong>g mitosis.[35] The same study also suggested that the presence of RCC2 is criticalfor the recruitment of other prote<strong>in</strong>s <strong>in</strong>volved <strong>in</strong> cell division to <strong>in</strong>ner centromeres <strong>in</strong>mitosis, supported by a <strong>no</strong>vel study which show that RCC2 is important for AURORA B (aprote<strong>in</strong> <strong>in</strong>volved <strong>in</strong> the chromosomal passenger complex) localization, but <strong>no</strong>t activity, <strong>and</strong>that RCC2-depleted extracts were impaired <strong>in</strong> their ability to align chromosomes to themetaphase plate.[36]These f<strong>in</strong>d<strong>in</strong>gs support the assumption that mutation <strong>in</strong> RCC2 can be associated withimproved survival as cells with this mutation will be arrested before cell division if themutation causes transcriptional <strong>in</strong>activation. The (A)10 repeat <strong>in</strong> RCC2 is located <strong>in</strong> exon 1which is <strong>in</strong> the 5’ untranslated region (UTR) of the gene, 76 bases upstream of the startcodon. UTRs are k<strong>no</strong>wn to affect mRNA nuclear export, cytoplasmic localization <strong>and</strong>translational efficiency <strong>and</strong> stability.[37] The majority of translational control occurs at thelevel of <strong>in</strong>itiation, thus implicat<strong>in</strong>g the 5’ UTR as a major site of translationalregulation.[38;39] It has also been shown that mo<strong>no</strong>nucleotide repeats <strong>in</strong> UTRs areconserved due to possible selective pressure relat<strong>in</strong>g to a functional role, <strong>and</strong> that theseconserved repeats are frequently altered <strong>in</strong> MSI-cancers, <strong>and</strong> so are speculated to be <strong>in</strong>volved<strong>in</strong> regulat<strong>in</strong>g gene expression.[40] Whether or <strong>no</strong>t the <strong>in</strong>dels seen <strong>in</strong> RCC2 arrest the cells22