Novel genetic and epigenetic alterations in ... - Ous-research.no

Figure 2. Mutation frequency differences between MSI-H and MSI-L tumors. Tumors with a low degreeof microsatellite instability (1 out of 5 consensus markers display instability) are less prone to acquire indelswithin repetitive sequences.Mutation verification by sequencing confirmed 95% of the mutations described withfragment analysis. Five of the 107 sequences differed from the fragment analysis results,yielding a false discovery rate of 5%. Figure 3 illustrates the comparisons of fragmentanalysis and dye-nucleotide sequencing.13