ab128503 PI3 Kinase Assay Kit - Abcam

4. Preparation of ReagentsA. Preparation of [γ 32 P] ATP MixPrepare a mix of ATP and [γ 32 P]-ATP to obtain an activity of 1 µCi ata concentration of 240 µM.10 µl [γ 32 P]-ATP Mix are required perassay point.B. Preparation of Lipid Substrate VesiclesEach Lipid Substrate vial contains the amount of PI required for thepreparation of 12 assay points. Fill up the Lipid Substrate vial with125 µl water per vial and vortex thoroughly. Sonicate the emulsionfor 1 hour in a water bath sonicator.6