14 JOURNAL OF APPLIED SEED PRODUCTION, VOL. 3, 1985assimilate was still down the plant to the vegetative tillers.Results from this trial suggest that even in non-lodged paclobutrazoltreated plants, the area of actively photosynthesisingtissue was insufficient to meet all the demands from thevarious sinks. Delaying leaf tissue senescence through fungicideapplication may have provided a greater photosyntheticsource and allowed more seeds per spikelet to be retainedthrough until harvest.The use of fungicides in the perennial ryegrass seed croprequires further investigation. Trials in both lodged and uprightcrops have demonstrated that sequential fungicide applicationsdelayed leaf tissue senescence in the absence ofleaf pathogens, and possible reasons for this have been previouslydiscussed (Hampton and Hebblethwaite, 1984).However, there is still little information available as to theeffects of pathogens on seed production (Labruyere, 1980),and the effects of single critically timed fungicide applicationson seed yield, as sequential applications are not financiallyviable. Recent work (Hampton, 1983; Hampton andHebblethwaite, 1985a) has suggested that the photosyntheticcapacity of the plant between ear emergence and anthesisstrongly influences the number of seeds retained per spikelet.The effects of fungicide application at this time need to beevaluated.ACKNOWLEDGEMENTSWe gratefully acknowledge the financial assistance andgrowth retardant supplied by ICI (Plant Protection Division)Plc.; the financial assistance and fungicide provided byBayer (U.K.) Ltd., the technical assistance of Mr. J.Travers, Mrs. S. Manison and other staff of the Agronomysection. J.G.H. also gratefully acknowledges the NewZealand National Research Advisory Council for the awardof a Fellowship to undertake the Ph.D. studies from whichthis paper originated.REFERENCES1. Anon. 197 6. Manual of plant growth stages and disease assessmentkeys. MAFF., Harpenden, Herts., U.K.2. Clemence, T.G.A., and P.D. Hebblethwaite. 1984. An appraisalof ear, leaf and stem t4C0 2 assimilation, '4C-assimilatedistribution and growth in a reproductive seed crop of amenityLolium perenne. Ann. App. Biol. 105:319-327.3. Evans, L.T., I.F. Wardlaw, and R.A. Fischer. 1975. Wheat.pp. 101-149 In L.T. Evans (ed.) Crop physiology-some casehistories. Cambridge University Press.4. Froggatt, P.J., W.D. Thomas and J.J. Batch. 1982. The valueoflodging control in winter wheat as exemplified by the growthregulator PP333. pp. 71-87 In A.F. Hawkins and B. Jeffcoat(eds.) Opportunities for manipulation of cereal productivity,Monograph 7. British Plant Growth Regulator <strong>Group</strong>, Wantage.5. Hampton, J.G. 1983. Chemical manipulation of Loliumperenne grown for seed production. Ph.D. thesis, University ofNottingham, U.K.6. Hampton, J.G., and P.D. Hebblethwaite. 1983. Yield componentsof the perennial ryegrass (Lolium perenne L.) seedcrop. J. Appl. <strong>Seed</strong> Production. 1:23-25.7. Hampton, J.G., and P.D. Hebb1ethwaite. 1984. The effect offungicide application on seed yield in perennial ryegrass cv.S24. Ann. Appl. Biol. 104:231-239.8. Hampton, J.G., and P.D. Hebblethwaite. 1985a. The effect ofthe growth regulator paclobutrazol (PP333) on the growth,development and yield of Lolium perenne grown for seed.Grass and Forage Sci. 40:93-101.9. Hampton, J.G., and P.D. Hebblethwaite. 1985b. The effect ofgrowth retardant application on floret site utilization and assimilatedistribution in ears of perennial ryegrass cv. S24. Ann.Appl. Bioi. 107:127-136.10. Hebblethwaite, P.D., D. Wright, and A. Noble. 1980. Somephysiological aspects of seed yield in Lolium perenne. pp.71-90 In P.D. Hebblethwaite (ed.) <strong>Seed</strong> production. Butterworths,London.11. Hebblethwaite, P.D., J.G. Hampton, and J.S.McLaren. 1982. The chemical control of growth, develop-mentand yield of Lolium perenne grown for seed. pp. 502-523 InJ. S. McLaren ( ed.) Chemical manipulation of crop growth anddevelopment. Butterworths, London.12. laggard, J.W., D.K. Lawrence, and P.V. Biscoe. 1982. Anunderstanding of crop physiology in assessing a plant growthregulator on sugar beet. pp. 139-150 In J.S. McLaren (ed.)Chemical manipulation of crop growth and development. Butterworths,London.13. Labruyere, R.E. 1980. Fungal diseases of grasses grown forseed. pp. 173-187 In P.D. Hebblethwaite (ed.) <strong>Seed</strong> production.Butterworths, London.14. Mohamed, G.E.S. and C. Marshall. 1979. Physiological aspectsof tiller removal in spring wheat. J. Agric. Sci., Cambridge.93:457-463.15. de Wit, C.T. 1965. Photosynthesis ofleafcanopies. Agric. Res.Rep. No. 633, Verslagen Landbouwk Onderzoet. pp. 1-57.16. W oledge, J. 1972. The effect of shading on the photosyntheticrate and longevity of grass leaves. Ann. Bot. 36:551-561.
The Effect of Time of Application of the Growth Retardant Flurprimidol (ELSOO) on<strong>Seed</strong> Yields and Yield Components in Lolium perenne L.rP.D. Hebblethwaite2, J.G. Hampton 3,s, G.R. Batts 4 and S. Barrett 3ABSTRACTThe effects of the growth retardant flurprimidol (ELSOO) ongrowth, development and yield of perennial ryegrass (Loliumperenne L.) following application at double ridge (DR), spikeletinitiation (SI) and floret initiation (FI) were investigated. AllELSOO applications significantly increased seed yields in bothyears. In 1983, no significant differences in stem length, lodging,dry matter accumulation, photosynthetic area index or seedyield were recorded between ELSOO application times. Howeverin 1984, lack of rain following the FI application delayed growthretardant activity so that lodging occurred before anthesis, andalthough fertile tiller numbers were eventually increased, seedyield was significantly lower than that for DR and SI applicationbecause of a reduction in the number of seeds per spikelet.DR application produced the greatest number of seeds perunit area in each year because of an increased production offertile tillers. Reasons for this are discussed. However, seed yieldwas not significantly different from that of other applicationtimes because of a failure to fill seeds to the same thousand seedweight.Additional index words: perennial ryegrass, seed production,double ridge, spikelet initiation, floret initiation.reduces internode enlongation of a broad range of both monocotyledonousand dicotyledonous plants (Anon., 1983).The mode of action involves a reduction in gibberellin biosynthesis.Hampton and Hebblethwaite (1985b) compared the effectsof EL500 and PP333 on perennial ryegrass seed yields andalso compared EL500 application rates (1.0 and 2.0 kg a.i.ha -1). In this paper we report the effects of time of EL500application on perennial ryegrass growth, development andseed yield.MATERIALS AND METHODSExperiments were carried out at the University of Nottinghamexperimental farm, Sutton Barrington, Loughborough,Leics., on soil of the Astley Hall series. Certified basic seedof perennial rye grass cv. S24 was sown in the autum of 1982and 1983 at 12 kg ha-t with a row width of 15 em and in plots1. 5 x 12m. Details of experimental management are given inTable 1.Table 1. Experimental details.INTRODUCTIONRecent reports of stem retardation and lodging preventionin the perennial' rye grass (Lolium perenne L.) seed crop havediscussed the effects of paclobutrazol (PP333)(Hebblethwaite et al., 1982; Hampton and Hebblethwaite,1985a). However, other products have also been investigated(Hampton, 1983), one of which is flurprimidol(EL500) = -( 1-Methylethyl)- -[ 4-(trifluoromethoxy) phenyl]-5-pyrimidine-methanol. Like paclobutrazol, flurprimidol isa foliar and root absorbed plant growth regulator whichSowing datePrevious cropHerbicide:AutumnSpring198323 August 1982PotatoesCambilene,4.91 ha-lNortron,9.81 ha-lNortron,4.91 ha-t198426 August 1983PotatoesCambilene,4.91 ha-tNortron,9.81 ha-l'Contribution from Department of Agriculture and Horticulture,School of Agriculture, University of Nottingham, Sutton Bonington,Loughborough, Leics., U.K. Received for publication 28 June1985.2Reader in Agronomy, 3Graduate Research Fellows and 4undergraduatestudent, University of Nottingham, School of Agriculture,Sutton Bonington, Loughborough, Leics., U.K.5Present Address: Official <strong>Seed</strong> Testing Station, Ministry ofAgriculture and Fisheries, P.O. Box 609, Palmerston North, NewZealand.Fertilizer:Application dateApplication rateELSOO application:Double ridgeSpikelet initiationFloret initiation<strong>Seed</strong> Harvest14 April120 kg N ha-l1 March29 March15 April15 July30 March120 kg N,60 kg P,60 kg K ha-l24 February28 March20 April18 July15