Dr. sc. Mladen Picer ASSESSMENT OF THE SELECTED POPs ...

DETAILS OF DESTRYED E.T.S.CROATIA

Kosovo

WAR

OrganisationnameRECETOX –TOCOEN &,Brno, CzechRepublicShort nameRECETOXMode ofcontractorContractorMaininvestigatorProf.dr.IvanHoloubekRUDJERBOSKOVIC,Zagreb,CroatiaRBIZ.CMRContractorDr. sc. M.Picer,Res.advGeologicalInstitute,Zagreb,CroatiaGEOZGBSubcontractorDr. sc. S.Kapelj, res.assoc.

Institute ofPublic Health,Zadar, CroatiaUniversityPristina, Facultyof Science,Pristina, Serbia& MontenegroIPHSZAD SubcontractorZ.DmitrovicB. Sc.FSCPRIDr. sc. T.SubcontractorArbneshi,DocentUniversity of NoviSad, Faculty ofSciences Instituteof Chemistry,Serbia &MontenegroUNS.FS.ICContractorProf. dr. M.VojinovicMiloradov

Univ. of Belgrade,Faculty of Miningand Geology,Serbia &MontenegroPublic HealthInstitute ofSerbia, Centre forEcotoxicologicalDiagnostic,Belgrade, Serbia& MontenegroFACMGBEO SubcontractorPUBHIBEOSubcontractorDr. sc. I.MatićDr.sc. D.Djarmati,Assoc.ProfGeological survey,Sarajevo, B&HGEOSURVContractorN. Miošić,B.Sc

EnvironmentalProtectionInstitute, Maribor,SloveniaIPHSI.EP.HSAContractorDr.sc. E.VončinaDemokritosUniversity ofThrace,Faculty ofEngineering,Xanthi GreeceUTHR.DEE.APLContractorDr. sc. S.RapsomanikisFederalEnvironmentAgency, Vienna,AustriaUMBContractorDr.sc. MartinKralik, Doc.

IPHSI.EP.HSA RECETOX UTHR.DEE.APL UNI.FS.IC & SUB.WP6 WP1 WP5WP2 WP4 WP3RBIZ.CMR & SUB GEOSURV UBM

IPHSI.EP.HSA UMASA.RECETOX UMBWP1 WP2A WP2BRBIZ.CMR & SUBCONTRACTORSGEOZGBIPHSZADFSCPRIWP2C WP4A WP 5WP6GEOSURV UTHR.DEE.APL UNS.FS.IC

IPHSI.EP.HSARECETOXWP1 WP3A WP3BUNS.FS.IC &SUBCONTRACTORSFACMGBEOPUBHIBEOWP4AWP 5RBIZ.CMRUTHR.DEE.APL

WORKPACKAGES AND CO-ORDINATORS OF ACTIVITIES• WP1: Intercalibration of some POPsanalysis in environmental samples (PhaseI and Phase II). Co-ordinator: JanaKlanova, Czech Republic

Intercalibration crm nist standardFigure 1: CRM NIS T18,0016,0014,0012,0010,001808,001536,001384,00118ce rtifie dUMASA.RECETOXRBIS .CMRIPHS I.EP.HSAUTHR.DEE.APLUNS.FS.ICPUBHEAL.CED2,000,001015228ng/ml

6000500040003000p,p´-DDT200010000p,p´-DDEIntercalibration crm SOILstandardFig ure 2: CRM AS LABLindan1801531381015228RBIS .CMRIPHS I.EP.HSAUTHR.DEE.APLUNS.FS.ICPUBHEAL.CEDUMASA.RECETOXce rtifie dng/g

WP2 : The level and hydrogeological fate of somePOPs in several Croatian, Bosnian & Herzegovinaand Kosovo areas as a consequence of wardamages: Co-ordinator: Mladen Picer, Croatia• WP2a: The level and hydrogeological fate ofsome POPs in several Croatian areas• Co-ordinator: S. Kapelj, Croatia• WP2b: The level and hydrogeological fate ofsome POPs in several Bosnian & Herzegovinaareas: Co-ordinator: N. Miošić, Bosnia &Herzegovina,• WP2c: The level of some POPs in severalKosovo areas. Co-ordinator: T. Arbneshi,Kosovo

Soil sample points at Zadar electro trafostation( 1 square is 1 m 2 :0 Zero point)

ETS ZADAR, ALL PCB data of samples withoutsample from the hole10 510 410 310 210 118161412108642 00PCB (ng/g)DISTANCE FROM ZERO POINT (m)51015202530DISTANCE FROM ZERO POINT (m)

ETS Zadar, soil samples collected 2003 (7 + 6 +3)4 mRESEARCHHOLE 1( 1,6 m depth)2 mRESEARCHHOLE 2( 1,6 m depth)1,6 mC1 C2 C3H1H2H31 m

LEVELS OF PCB IN SOIL SAMPLES FROM THE OUTERRESEARCH HOLE(ANALYSIS PERFORMED BY IPHSI.EP.HSA, MARIBOR)1000LEVEL OF PCB CONGENERS(mg/Kg) d.w.1001010,10,01SUM 7 CONG.PCB 28PCB 520,0010 0,2 0,4 0,6 0,8 1 1,2DEPTH OF RESEARCH HOLE (m)

WP3: The level and hydrogeological fate ofsome POPs in several FR Yugoslavian areas asa consequence of war damages. Co-ordinatorI. Matić, Serbia and Montenegro• WP3a :The level and hydrogeological fate ofsome POPs in several Serbia areas. Co-ordinatorD. Djarmati, Serbia and Montenegro• WP3b: The level and hydrogeological fate ofsome POPs in several Vojvodina areas. CoordinatorM. Vojinovic-Miloradov, Serbia andMontenegro

PCBs(ppm)1 / 4,400PCDD/Fs(ppt)74,000 / 10,200

4 3 2 1• A-1• B-4 • B-3 • B-2• B-5• B-1AB• C-1Sewage collectorCDSampling locations : A-1(0.4 m), B-2(0.6 m), B-1(0.2 m), B-4(0.4 m), B-3(0.2 m), B-5(1.0 m) andC-1 (0.2 m)

Content of total PCBs in the sampling spotsng/g40003500300025002000150010005000(0.4 m) (0.2 m) (1.0 m) (0.2 m) (0.6 m) (0.2 m) (0.4 m)A-1 B-1 B-5 C-1 B-2 B-3 B-4Sampling spots

WP4 :The level and atmospheric transportation of somePOPs in Croatian, Bosnian & Herzegovina, and Serbian& Montenegro areas as consequence of war damages.Co-ordinator: Ivan Holoubek, Czech RepublicWP4a: The level and atmospheric transportation ofsome POPs in Croatian, and Bosnian &Herzegovina, areas. Co-ordinators: I. Holoubekand M. Picer, Czech Republic and Croatia• WP4b: The level and atmospheric transportationof some POPs in FR Yugoslavian areas. Coordinators:S. Rapsomanikis and D. Djarmati,Greece and Serbia&Montenegro

Air sampling campaign in CroatiaMay 13-23, 2003, ZadarZadar – historical centerGPS: N 44°06’45,5“ E 15°13’43,3“ 33 mZadar – Adria, industrial zone (kod CA)GPS: N 44°05’47,6“ E 15°15’38,9“ 1 mZadar – transformer station between industrial and residential areaGPS: N 44°06’23,9“ E 15°15’48,9“ 18 mZadar – meteorological stationGPS: N 44°07’48,3“ E 15°12’21,2“ 6 m

PCBs levels in ambient airAir sampling campaign in CroatiaMay 13-23, 2003, Zadar0,2500,2007 PCB LEVEL(ng/m 3 )0,1500,1000,050ZavizanMeteoAdriaCentre-CentreAdriaMeteoZavizan1 2 3 4 5 6 7 8 9 10DAYS

PCBs levels in ambient air(ng/m 3 )Air sampling campaign in CroatiaMay 13-23, 2003, Zadar6,005,004,003,002,001,00ZavizanMeteoAdriaCentreTrafo0,0012345678910TrafoCentreAdriaMeteoZavizan

WP 5:• Investigation of intake and someecotoxicological consequence ofexposure of living organisms toPOPs in some Serbian &Montenegroand Croatian areas as consequenceof war damages. Co-ordinator: M.Vojinovic-Miloradov, Serbia&Montenegro

Interview questions• Average consumption of total freshwaterfish (Kg/month) for all family• Average consumption of total sea fish(Kg/month) for all family• Average consumption of total sea fishhatched from Zadar coastal area(Kg/month) for all family• List three species of fish the mostconsumables• Name and profession of person whomade interview

FISH CONSUPTION OF 50 INTERVIEWEDFISHERMEN FAMILIES876543210CONS. T.F.CONS. ZAD. F..CROAT AVER.FISH CONSUPTION PER PERSON PER MONTH(kg)135791113151719212325272931333537394143454749NTERVIEWED FISHERMEN FAMILY

Micro-EROD bioanalysis of soil samples (P-2, P-6) in primaryrat hepatocytes culture.GC/ECDPCBs:

PyraleneP-2P-6Estimated EC 25PCB126 and bioTEQEC 25PCB1260.84 µg / 0.1 ml64 mg / 0.1 ml16 mg / 0.1 mlbio-TEQng/g107.841.425.71Values were calculated using maximumEROD activity in primary hepatocytes cultureinduced by 10 -8 M PCB 126GC/ECDPCBs: 4.5 ppmGC/ECDPCBs: 3.9 ppmIn spite that results obtained byGC/ECD demonstrated absence ofPCBs in soil samples P-2 and P-6,micro-EROD analysis indicates thepresence of relatively high amountof EROD-inducing compounds inthe investigated samples (bio-TEQvalues 1.4 and 5.7 ng/g).On the other hand, GC/ECDdemonstrated that sediment samples(Z1-0.4, Z1-0.2) contained ppmlevels of PCBs, and in accordancewith that, micro-EROD analysisindicate the presence of very highamount of EROD-inducingcompounds in the investigatedsamples. It suggest that besidesPCBs, some other dioxin-likecompounds are possible present inthe soil samples.

WP 6:• Laboratory and field PCBs biotransformationstudies for remediation of soil contaminated bytransformer station oil-spill. Co-ordinator: D.Hršak, Croatia

Enrichment of biphenyl-degradingmicrobial communities– microcosm enrichment approach was employed– microbial growth was stimulated by incubating soil withbiphenyl– top soil layer (0-5 cm) from PCB polluted sites were used:- 7 samples at different positions of the transformerstation area- 2 samples at Omišalj and Trogir airports, Croatia– biphenyl-degrading bacteria were isolated on biphenyl-MMagar plates

Figure 1.Remaining congeners in PCB50 mixture (initialconcentration 50 ppm) after 14-day cultivation ofselected culture originated from airport soil,TrogirAIR-TR1.experiment 12.05.03 2.experiment 05.06.03.1009080% of remaining peak7060504030201001 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46peak

Figure 2.Remaining congeners in PCB50 mixture (initialconcentration 50 ppm) after 14-day cultivation ofselected culture originated from power substationsoil, ZadarTS Z71.experiment 12.05.03. 2.experiment 05.06.03.10090807060504030201001 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46peak

LABORATORY REMEDIATIONEXPERIMENT- BOTTLES

LABORATORY REMEDIATION1) CONTROL(STERILIZED SOILTSZ)2) BACT.TSZ-23) BACT.TSZ-64) BACT.TROGIR5) BACT.OMIŠALJ6) AUTOCHTONEBACTERIES IN TSZSOILS

LABORATORY REMEDIATIONEXPERIMENT ON ETS ZADAR SOIL SPIKEDWITH 100 PPM AROCLOR 1248140120AROCLOR 1248 IN SOIL (mg/kg)10080604020XEXT IEXT IISOXHSUM(MEAN)SUM0K I K II 1 I 1 II 2 I 2 II 3 I 3 II 4 I 4 II 5 I 5 IICONTROLBACT.TSZ-2 BACT.TSZ-6BACT.TROGIR BACT.OMIŠALJ AUTOCHTONEMODE OF LABORATORY REMEDIATION PROCESS

TAKE AWAY THE MOST POLLUTED(WE HOPE) SOIL

EXPERIMENTAL FIELDS (PHASEII –FILLING THE OPEN BOXES)