Agilent 2100 Bioanalyzer System - K'(Prime) Technologies

Agilent 2100 Bioanalyzer and Agilent 2200 TapeStation SystemsAgilent 3100 OFFGEL FractionatorSYSTEMS, CONSUMABLES AND SUPPLIES

Agilent 2100 Bioanalyzer SystemOne platform – Endless possibilitiesThe Agilent 2100 Bioanalyzer system offers fast and reliable separation, sizing and quantifi -cation of DNA, RNA and proteins by miniaturized on-chip electrophoresis surpassing laborintensive slab gels by speed, reproducibility and independence from user infl uences.A unique feature of the Agilent 2100 Bioanalyzer system allows the instrumnet to easilyswitch from electrophoresis to two-color fl ow cytometry analysis of fl uorescently-labeledcells. This versatility makes the Agilent 2100 Bioanalyzer system an indispensable tool forthe molecular biologist and biochemist.Key benefits• Industry standard for DNA quality control in next generation sequencing workflows.• RNA Integrity Number (RIN) is the gold standard for RNA integrity measurements.• Highest sensitivity with detection in the picogram range for DNA, RNA, andprotein analysis.• Highest assay accuracy and precision.• Minimal sample consumption (1 μL for nucleic acids and 4 μL for diluted proteins)with results in as little as 25 minutes.• Replaceable electrode cartridge can be easily exchanged for contamination-free switchof methods.• Digital data with various data display options – gel view, electropherograms,and tables – allows convenient analysis, archiving, and simple comparison of samplesfrom multiple chips.• Multiple exportable data formats (xml, csv, html, pdf, wmf, jpg, tif, bmp, and aia).• Minimum exposure to hazardous materials.• Supports 21 CFR Part 11 compliance.Ensuring high quality RNA for downstream applicationsThe Agilent 2100 Bioanalyzer system is the gold standard for integrity measurementson RNA. In less than 30 minutes, small RNA or total RNA is checked for quantity anddegradation. The unique RIN offers a measure for normalized RNA quality to assurereproducible results from downstream experiments, such as next generation sequencing,microarrays or qPCR.RIN:Incubation time (increasing degradation of RNA)Time (seconds)2

Agilent 2100 Bioanalyzer SystemThe Agilent 2100 Bioanalyzer systemis a versatile system for sizing,quantifi cation and quality controlof DNA, RNA, proteins and cellson a single platform.Agilent 2100 Bioanalyzer SystemsPart Number Description QuantityG2940CAG2943CAG2939AAG2947CAG2948CAG2938C2100 Bioanalyzer Desktop BundleIncludes the Bioanalyzer instrument, desktop PC with 2100 Expert software preinstalled, instrument license, printer, chippriming station, chip vortexer, and accessories.NOTE: Does not include cartridge. Requires Electrophoresis Set (G2947CA) or Flow Cytometry Set (G2948CA) for operation.2100 Bioanalyzer Laptop BundleIncludes the Bioanalyzer instrument, laptop PC with 2100 Expert software preinstalled, instrument license, and printer, chippriming station, chip vortexer, and accessories.NOTE: Does not include cartridge. Requires Electrophoresis Set (G2947CA) or Flow Cytometry Set (G2948CA) for operation.2100 Electrophoresis Bioanalyzer InstrumentFor electrophoretic assays only.Includes the electrophoresis-only Bioanalyzer instrument, electrode cartridge, 2100 Expert software and licenses, chip primingstation, chip vortexer, test chips, accessories, and installation and familiarization services.NOTE: Does not include PC.Electrophoresis SetIncludes the electrode cartridge, electrophoresis license, test chips, and installation and familiarization services.Flow Cytometry SetIncludes the pressure cartridge, fl ow cytometry license, cell check-out kit, test chip, and installation and familiarization services.2100 Bioanalyzer Add-on InstrumentThe Bioanalyzer instrument only. Ideal for experienced users requiring the addition of a second instrument to their system. TwoBioanalyzer instruments may be operated from one PC.1 system1 system1 system1 set1 set1 instrumentSoftwarePart Number Product QuantityG2946CAG2949CA2100 Expert Software UpgradePackage for upgrade to the latest revision of 2100 Expert software.Includes the required license keys to run the instrument.2100 Expert Security Pack LicenseFor CFR 21 Part 11 compliance.Includes compliance software upgrade and licenses.1 upgrade1 license- 2100 Expert Data Review SoftwareSoftware for analysis of data generated by the 2100 Bioanalyzer system. No purchase or licenses required.Download free of charge from: www.agilent.com/genomics/bioanalyzer3

Agilent 2100 Bioanalyzer SystemAccessories and Spare PartsPart Number Description Quantity5065-9951 Electrode Cleaner KitIncludes additional electrode cleaners for the maintenance of the electrode cartridge.G2938-68300 Test Chip KitFor running instrument diagnostics and troubleshooting electrophoretic assays.Includes autofocus chip, electrode/diode test chip, and documentation.G2938-68200 Cell Test Chip KitFor running instrument diagnostics and troubleshooting cell assays.Includes cell autofocus chip and documentation.5065-4478 Pressure Adapter KitFor use with pressure cartridge.Includes 1 mounting ring and 5 pressure adapters with gasket.5065-4401 Chip Priming StationUsed to load gel matrix into a chip with a syringe provided in each assay kit – used for RNA, DNA, and protein assays.Includes priming station, timer, and 1 syringe clip.5042-1398 Adjustable Clip for Priming StationUsed in combination with a syringe to apply defi ned pressure for chip priming.G2938-68716 Gasket Kit for Chip Priming StationIncludes 1 syringe adapter, 10 gaskets, and 1 mounting ring.5185-5990 Filters for Gel MatrixExtra spin fi lters for the gel matrix in RNA, DNA, and protein assays.2110-0007 Fuse for 2100 Bioanalyzer Power Supply1 A / 250 V.7 electrodecleaners1 kit1 kit1 kit1 kit1 clip1 kit25 fi lters1 fuseRS232-61601RS-232 CableConnector cable between desktop or laptop PC and Agilent 2100 Bioanalyzer.1 cable8121-1013 USB Serial Adapter CableConnects RS-232 cables to USB PC ports (for PCs without serial ports).5065-9966 Vortex Mixer AdapterDesigned to hold chip fi rmly in place while vortexing. Replacement part for IKA MS-2 vortexer (3 mounting screws).1 cable1 adapterServicesFor more details on our service portfolio, please contact your sales representative orAgilent’s Worldwide Sales and Support Phone Assistancewww.agilent.com/genomics/contactus4

DNA SolutionsThe Agilent DNA kits, together withthe Agilent 2100 Bioanalyzer system,are ideal for automated sizing andquantifi cation of PCR fragments,restriction digests or fragmented DNA.DNA Kits and ReagentsPart Number Description Quantity5067-4626 High Sensitivity DNA KitFor the separation, sizing and quantifi cation of dsDNA samples of very low abundance and range from 50 to 7000 bp.Includes 10 chips, reagents, ladder and consumables.NOTE: Only compatible with G2938B, G2938C and G2939A Bioanalyzer instruments.5067-4627 High Sensitivity DNA ReagentsIncludes reagents and ladder; no chips.5067-1504 DNA 1000 KitFor sizing and quantifi cation of dsDNA fragments from 25 to 1000 bp.Includes 25 chips, reagents, ladder and consumables.5067-1505 DNA 1000 ReagentsIncludes reagents and ladder; no chips.5067-1506 DNA 7500 KitFor sizing and quantifi cation of dsDNA fragments from 100 to 7500 bp.Includes 25 chips, reagents, ladder and consumables.5067-1507 DNA 7500 ReagentsIncludes reagents and ladder; no chips.5067-1508 DNA 12000 KitFor sizing and quantifi cation of dsDNA fragments from 100 to 12000 bp.Includes 25 chips, reagents, ladder and consumables.5067-1509 DNA 12000 ReagentsIncludes reagents and ladder; no chips.For 110 samplesFor 10 chipsFor 300 samplesFor 25 chipsFor 300 samplesFor 25 chipsFor 300 samplesFor 25 chips5

DNA SolutionsDNA SpecificationsAnalytical Specifications High Sensitivity DNA DNA 1000 DNA 7500 DNA 12000Sizing range 50–7000 bp 25–1000 bp 100–7500 bp 100–12000 bpSizing resolution 50–600 bp: ±10 %600–7000 bp: ± 20 %25–100 bp: 5 bp100–500 bp: 5 %500–1000 bp: 10 %100–1000 bp: 5 %1000–7500 bp: 15 %100–1000 bp: 5 %1000–12000 bp: 15 %Sizing accuracy ± 10 % CV* ± 10 % CV* ± 10 % CV* ± 15 % CV*Sizing reproducibility 5 % CV* 5 % CV* 5 % CV* 5 % CV*Quantification accuracy 20 % CV* 20 % CV* 20 % CV* 25 % CV*Quantification reproducibility50–2000 bp: 15% CV*2000–7000 bp: 10% CV*25–500 bp: 15 % CV*500–1000 bp: 5 % CV*100–1000 bp: 10 % CV*1000–7500 bp: 5 % CV*100–1000 bp: 15 % CV*1000–12000 bp: 10 % CV*Quantitative range 5–500 pg/µL* 0.5–50 ng/µL* 0.5–50 ng/µL* 0.5–50 ng/µL*Maximum sample bufferstrengthPhysical Specifications10 mM Tris1 mM EDTA250 mM for KCl250 mM for KCl250 mM for KCl15 mM for MgCl 215 mM for MgCl 215 mM for MgCl 2250 mM for NaCl250 mM for NaCl250 mM for NaClAnalysis time 45 minutes 35 minutes 30 minutes 30 minutesSamples per chip 11 12 12 12Sample volume 1 µL 1 µL 1 µL 1 µLKit stabilityMinimum 4 months(see box for storage temp.)Minimum 4 months(see box for storage temp.)Minimum 4 months(see box for storage temp.)Minimum 4 months(see box for storage temp.)Kit size DNA 11/chip =110 samples/kitDNA 12/chip =300 samples/kitDNA 12/chip =300 samples/kitDNA 12/chip =300 samples/kit* Determined by analyzing the DNA ladder as sampleDNA Application NotesPublication NumberDescription5989-3514EN Measuring the METH-2 promoter hypermethylation and transcript downregulation in non-small cell lung carcinomas with the Agilent 2100Bioanalyzer5989-6836EN5990-3948EN5990-4942EN5990-5008EN5990-8382ENUse of the Agilent 2100 Bioanalyzer for basmati rice authenticity testingComplete automation of the Stratagene Strata Prep 96 PCR Purifi cation kit with the Agilent Bravo Automated Liquid Handling platform andAgilent Automated CentrifugeAutomation of Agencourt AM Pure Purifi cation kit for the purifi cation of Next-Generation Sequencing sample preparation reactions on BravoImproving sample quality for target enrichment and next-gen sequencing with the Agilent High Sensitivity DNA kit and the AgilentSureSelect Target Enrichment platformLow input DNA size selection on the Pippin Prep System using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kitThis list only provides an overview of selected DNA application notes. Visit our website atwww.agilent.com/genomics/bioanalyzer for a complete list of all available application and technical notes.Or, see the Agilent 2100 Bioanalyzer – Application Compendium (Publication Number 5990-6987EN).6

RNA SolutionsThe Agilent RNA kits and RNA IntegrityNumber (RIN) are the industry standard forRNA quality assessment. Perform fast, easyand precise integrity checks and samplequantifi cation before any RNA-dependentapplication.RNA Kits and ReagentsPart Number Description Quantity5067-1511 RNA 6000 Nano KitFor analysis and quantifi cation of total RNA and mRNA samples of 25 to 500 ng/µL in concentration.Includes 25 chips, reagents, ladder and consumables.5067-1512 RNA 6000 Nano ReagentsIncludes reagents and ladder; no chips.5067-1529 RNA 6000 Nano LadderIncludes ladder only.5067-1513 RNA 6000 Pico KitFor the analysis of RNA samples of low abundance down to 50 pg/µL of total RNA or 250 pg/µL of mRNA.Includes 25 chips, reagents, ladder and consumables.5067-1514 RNA 6000 Pico ReagentsIncludes reagents and ladder; no chips.5067-1535 RNA 6000 Pico LadderIncludes ladder only.5067-1548 Small RNA KitFor the analysis and quantifi cation of small RNA samples ranging from 6 to 150 nt in size and 50 to 2000 pg/µL in concentration.Includes 25 chips, reagents, ladder and consumables.5067-1549 Small RNA ReagentsIncludes reagents and ladder; no chips.5067-1550 Small RNA LadderIncludes ladder only.For 300 samplesFor 25 chipsFor 25 chipsFor 275 samplesFor 25 chipsFor 25 chipsFor 275 samplesFor 25 chipsFor 25 chips7

RNA SolutionsRNA SpecificationsAnalyticalSpecificationsRNA 6000 NanoTotal RNARNA 6000 NanomRNARNA 6000 PicoTotal RNARNA 6000 PicomRNASmall RNAQuantitative range 25–500 ng/μL 25–250 ng/μL - - 50–2000 pg/μLof purifi ed miRNA inwaterQualitative range 5–500 ng/μL 25–250 ng/μL 50–5000 pg/μLin water250–5000 pg/μLin water50–2000 pg/μLof purifi ed miRNA inwaterSizing range - - - - 6-150 ntSensitivity(signal/noise > 3)5 ng/μL in water 25 ng/μL in water 50 pg/μL in water200 pg/μL in TE250 pg/μL in water500 pg/μL in TE50 pg/μL in water**Quantificationreproducibility(within a chip)Quantificationaccuracy*10 % CV 10 % CV 20 % CV 20 % CV 25 % CV20 % 20 % 30 % 30 % -Maximum samplebuffer strength100 mM Tris0.1 mM EDTAor 125 mM NaClor 15 mM MgCl 2100 mM Tris0.1 mM EDTAor 125 mM NaClor 15 mM MgCl 250 mM Tris0.1 mM EDTAor 50 mM NaClor 15 mM MgCl 250 mM Tris0.1 mM EDTAor 50 mM NaClor 15 mM MgCl 210 mM Tris0.1 mM EDTAPhysical SpecificationsAnalysis time 30 minutes 30 minutes 30 minutes 30 minutes 30 minutesSamples per chip 12 12 11 11 11Sample volume 1 μL 1 μL 1 μL 1 μL 1 μLKit stabilityMinimum 4 monthsat 4 °CMinimum 4 monthsat 4 °CMinimum 4 monthsat 4 °CMinimum 4 monthsat 4 °CMinimum 4 monthsat 4 °CKit sizeRNA Nano 12/chip= 300 samples/kitRNA Nano 12/chip= 300 samples/kitRNA Pico 11/chip= 275 samples/kitRNA Pico 11/chip= 275 samples/kitSmall RNA 11/chip= 275 samples/kit* Determined analyzing the RNA ladder as sample** Measured for the 40 nt fragment of the Small RNA ladderRNA Application NotesPublication NumberDescription5989-1165EN5989-7730EN5989-7870EN5989-8539EN5990-3558EN5990-3917EN5990-5557EN5990-8850ENRNA Integrity Number (RIN) – Standardization of RNA quality controlOptimizing real-time quantitative PCR experiments with the Agilent 2100 BioanalyzerAnalysis of miRNA content in total RNA preparations using the Agilent 2100 BioanalyzerAnalysis of small RNAs from Drosophila Schneider cells using the Small RNA assay on the Agilent 2100 BioanalyzerAutomation of Stratagene Absolutely RNA 96 Microprep kit with the Bravo Automated Liquid Handling platformGene expression microarray analysis of archival FFPE samplesRNA quality control in miRNA expression analysisAssessing integrity of plant RNA with the Agilent 2100 BioanalyzerThis list only provides an overview of selected RNA application notes. Visit our website atwww.agilent.com/genomics/bioanalyzer for a complete list of all available application and technical notes.Or, see the Agilent 2100 Bioanalyzer – Application Compendium (Publication Number 5990-6987EN).8

Protein SolutionsThe Agilent Protein kit portfolio provides afast and fl exible way for the assessment ofprotein concentration, identity, and purity ina wide variety of samples.Protein Kits and ReagentsPart Number Description Quantity5067-1515 Protein 80 kitFor sizing and quantifi cation of protein samples from 5 to 80 kDa.Includes 25 chips, reagents, ladder and consumables.5067-1516 Protein 80 reagentsIncludes reagents and ladder; no chips.5067-1517 Protein 230 kitFor sizing and quantifi cation of protein samples from 14 to 230 kDa.Includes 25 chips, reagents, ladder and consumables.5067-1518 Protein 230 reagentsIncludes reagents and ladder; no chips.5067-1575 High Sensitivity Protein 250 kitFor sizing and highly sensitive quantifi cation of protein samples from 10 to 250 kDa.Includes 10 chips, analysis and labeling reagents, and consumables.NOTE: Only supported for G2938B, G2938C and G2939A Bioanalyzer instruments.5067-1576 High Sensitivity Protein 250 reagentsIncludes reagents for Bioanalyzer analysis; no chips.5067-1577 High Sensitivity Protein 250 labeling kitIncludes reagents for labeling reaction.5067-1578 High Sensitivity Protein 250 ladderIncludes ladder.For 250 samplesFor 25 chipsFor 250 samplesFor 25 chipsFor 100 samplesFor 10 chipsFor 100 samplesFor 10 chips9

Protein SolutionsProtein SpecificationsAnalytical specifications Protein 80 Protein 230 High Sensitivity Protein 250Sizing range 5–80 kDa 14–230 kDa 10–250 kDaTypical sizing resolution 10 % 10 % 10 %Typical sizing accuracy 10 % CV (CAII, BLG) 10 % CV (BSA, CAII) 10 % CV (BSA)Sizing reproducibility 3 % CV (CAII, BLG) 3 % CV (BSA, CAII) 3 % CV (BSA)Sensitivity(signal/noise > 3)6 ng/μL CAII in PBS15 ng/μL BSA in PBS10 ng/μL CAII in 0.5 M NaCl30 ng/μL BSA in 0.5 M NaCl6 ng/μL CAII in PBS15 ng/μL BSA in PBS30 ng/μL BSA in 0.5 M NaCl1 pg/μL labeled BSA in water on chip5 pg/μL labeled BSA in PBS on chip(Labeling reaction at 1 ng/μL of totalprotein)Quantitative range 60–2000 ng/μL CAII in PBS 15–2000 ng/μL CAII in PBS30–2000 ng/μL BSA in PBSQualitative range 6–4000 ng/μL CAII and BLG 6–5000 ng/μL CAII in PBS15–5000 ng/μL BSA in PBS0.3–3000 ng/μL BSA-Quantification reproducibility 20 % CV (CAII, BLG) 20 % CV (BSA, CAII) 20 % CV (BSA)Physical specificationsAnalysis time 30 minutes 25 minutes 30 minutesSamples per chip 10 10 10Sample volume 4 μL 4 μL 5 μLKit stabilityMinimum 4 months(see box for storage temp.)Minimum 4 months(see box for storage temp.)Minimum 6 months at -20 °CKit sizeProtein 10/chip= 250 samples/kitProtein 10/chip= 250 samples/kitProtein 10/chip= 100 samples/kitCAII = carbonic anhydrase, BSA = bovine serum albumin, BLG = beta-lactoglobulinProtein Application NotesPublication Number5989-7735EN5989-8419EN5990-4097EN5990-8125ENDescriptionRapid wheat varietal identifi cation using the Agilent 2100 Bioanalyzer and automated pattern-matchingAlternative to 2D gel electrophoresis – OFFGEL electrophoresis combined with high-sensitivity on-chip protein detectionImmunoprecipitation and the High Sensitivity Protein 250 assayMilk protein analysis with the Agilent 2100 Bioanalyzer and the Agilent Protein 80 kitThis list only provides an overview of selected protein application notes. Visit our website atwww.agilent.com/genomics/bioanalyzer for a complete list of all available application and technical notes.Or, see the Agilent 2100 Bioanalyzer – Application Compendium (Publication Number 5990-6987EN).10

Cell SolutionsThe Agilent Cell Fluorescence kits areideally suited for easy, fast, and automatedtwo-color fl ow cytometry analyses offl uorescently labeled cells.Cell KitsPart Number Description Quantity5067-1519 Cell kitFor analysis of fl uorescently stained cells. Includes 25 chips, reagents and consumables.5067-1520 Cell checkout kitFor testing proper fl ow cytometry operation. Includes 7 cell chips and reagents.For 150 samplesFor 7 chipsCell SpecificationsCell Application NotesAnalytical SpecificationsCellPublication NumberDescriptionRed channelExcitation range 620–645 nm (maximum 630 nm)Emission range 674–696 nm (maximum 680 nm)5988-4319ENApoptosis detection by annexin V and activecaspase-3 with the Agilent 2100 BioanalyzerBlue channelExcitation range 458–482 nm (maximum 470 nm)Emission range 510–540 nm (maximum 525 nm)5988-4320ENMonitoring transfection effi ciency by greenfl uorescence protein (GFP) detectionDetection limit5,000 MESF (red channel)2,000,000 MESF (blue channel)5988-4322ENDetection of antibody-stained intracellular proteintargets with the Agilent 2100 BioanalyzerMinimum cell throughput 2.5 cells / secondRecommended cell density 2.0 x 10 6 / mL (expected counts = 500–1,000 cells)Physical SpecificationsAnalysis time30 minutesSamples per chip 6Sample volume 10 μL (recommended number of cells 2 x 10 4 )Kit stability Minimum 4 months at 4 °CKit sizeCell 6/chip = 150 samples/kit5989-2718EN5989-2934EN5989-7171ENCytometric analysis of upregulated functional geneexpression in primary cells by on-chip stainingDetection of apoptosis in primary cells by annexin Vbinding using the Agilent 2100 BioanalyzerIdentification of red and white blood cells from wholeblood samples using the Agilent 2100 BioanalyzerThis list only provides an overview of selected cell application notes.Visit our website at www.agilent.com/genomics/bioanalyzerfor a complete list of all available application and technical notes.Or, see the Agilent 2100 Bioanalyzer – Application Compendium(Publication Number 5990-6987EN).11

Agilent 2200 TapeStation SystemAccelerate sample QC with confidenceThe Agilent 2200 TapeStation system offers fast and reliable RNA, DNA and proteinelectrophoresis with scalable throughput, making it an ideal solution for quality control ofbiological samples in next-generation sequencing (NGS), microarray and qPCR workfl ows,as well as in protein purifi cation and antibody production.Sample analysis has never been so easy – simply load the 2200 TapeStation instrumentwith the credit card-sized, disposable ScreenTape, fi ltered loading tips, and your samplesin 16 tube strips or 96-well plates – and you will be reviewing results in approximately1 minute per sample.Key benefits• Up to 96 samples are analyzed with constant costs per sample providingscalable throughput.• Results are obtained in approximately one minute per sample, even for 96 samples.• Simplify your workflow with ready-to-use ScreenTape consumables and automation.• Achieve user-independent results with minimal manual intervention and excellentreproducibility.• Requires not more than 2 µL of DNA, RNA or proteins samples, even for highsensitivity analysis.• Switch with ease between DNA, RNA and protein ScreenTape for greatest fl exibility.• Rely on the market-leading RNA quality standard (RIN) by using the AgilentdevelopedRINe.• Carryover is eliminated with individual loading tips for each sample, the ScreenTapeanalyses each sample in a separate lane.DNA analysis during the NGS workflowThe 2200 TapeStation system was used at several quality control steps of the NGSworkfl ow: shearing of genomic DNA, library amplifi cation, and post-capture amplifi edlibrary after SureSelect targeted enrichment. The fi gure shows post-sheared genomicDNA analyzed on D1K ScreenTape.60600MW[bp] A0 (L) A14000150010007003000500300100Sample intensity [FU]200010002502510030050070010001500MW[bp]12

Agilent 2200 TapeStation SystemThe compact Agilent 2200 TapeStationsystem automates RNA, DNA and proteinsample QC, including sample loading,separation, and imaging, with scalablethroughput.Agilent 2200 TapeStation SystemsPart Number Description QuantityG2964AAG2965AA2200 TapeStation SystemFor RNA, DNA and protein analysis.Includes the 2200 TapeStation instrument, laptop with 2200 TapeStation software, accessories, consumables anduser information.2200 TapeStation Nucleic Acid SystemFor RNA and DNA analysis only.Includes the 2200 TapeStation Nucleic Acid instrument, laptop with 2200 TapeStation software, accessories,consumables and user information.1 system1 systemAccessories and Spare PartsPart Number Description Quantity5067-5155 Sample block (for tube strips) 1 block5067-5156 Sample block (for 96 well plate) 1 block5067-5158 Loading tip holder 1 holder5067-5150 96-well sample plates 10 plates5067-5154 96-well plate foil seal 100 foils5067-5152 Loading tips, 10 Pk 10 packs of 384 tips each5067-5153 Loading tips, 1 Pk 1 pack of 384 tips401428 Optical tube strips, 8x strip 1 box of 120401425 Optical caps, 8x strip 1 box of 120ServicesFor more details on our service portfolio, please contact your sales representative orAgilent’s Worldwide Sales and Support Phone Assistancewww.agilent.com/genomics/contactus13

DNA SolutionsThe D1K ScreenTape provides separationand analysis of DNA fragments, fragmentedDNA and NGS libraries from 35 bp to1000 bp.DNA Consumables and ReagentsPart Number Description Quantity5067-5361 D1K ScreenTapeFor the analysis of DNA from 35 to 1000 bp. Includes 7 ScreenTape consumables.5067-5362 D1K ReagentsFor the analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer.5067-5363 High Sensitivity D1K ScreenTapeFor the high sensitivity analysis of DNA from 35 to 1000 bp. Includes 7 ScreenTape consumables.5067-5364 High Sensitivity D1K ReagentsFor the high sensitivity analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer.For 112 samplesFor 112 samplesFor 112 samplesFor 112 samplesDNA SpecificationsDNA Application NotesAnalytical Specifications D1K ScreenTape High SensitivityD1K ScreenTapeSizing range 35–1000 bp 35–1000 bpPublication Number5990-8760ENDescriptionD1K ScreenTape simplifi es mycobacteriumtuberculosis genotypingResolution 1 35–300 bp: 15 %300–1000 bp: 10 %35–300 bp: 15 %300–1000 bp: 10 %5990-9051ENHLA typing analysis using D1K ScreenTape is fast,effi cient and safeSensitivity 2 0.05 ng/µL 5 pg/µL 3Sizing precision 5 % CV 5 % CVSizing accuracy 4 ± 10 % ± 10 %Quantitative precision 10 % CV 15 % CVQuantitative accuracy ± 20 % ± 20 %Quantitative range 0.1–50 ng/µL 75–1000 pg/µL5990-8761END1K ScreenTape allows mycoplasma PCR analysisfor cell line screeningThis list only provides an overview of selected DNAapplication notes. Visit our website atwww.agilent.com/genomics/tapestation for acomplete list of all available application and technical notes.Physical SpecificationsAnalysis time16 samples< 20 minutes96 samples ~100 minutes16 samples< 20 minutes96 samples ~100 minutesSamples per consumable 16 16Sample volume required 1 μL 2 µLKit stability 4 months 4 monthsKit size 112 samples/box 112 samples/box1. Resolution is defi ned as the separation of fragments at half peak height or better.2. Signal:noise ratio >3 for a single peak3. 2200 TapeStation Nucleic Acid System (G2965AA)4. Determined using the ladder as sample14

RNA SolutionsThe R6K ScreenTape provides a fullyautomated, effi cient and reliable RNAanalysis for RNA characterization andquality assessment. The RNA integritynumber equivalent (RINe) provides aninstant and objective evaluation ofeukaryotic total RNA degradation.RNA Consumables and ReagentsPart Number Description Quantitiy5067-5367 R6K ScreenTapeFor analysis of RNA down to a sensitivity of 2 ng/µL. Includes 7 ScreenTape consumables.5067-5368 R6K ReagentsFor the analysis of RNA from 25 to 500 ng/µL. Includes sample buffer.5067-5369 High Sensitivity R6K ScreenTapeFor the high sensitivity analysis of RNA down to 100 pg/µL. Includes 7 ScreenTape consumables.5067-5370 High Sensitivity R6K ReagentsFor the high sensitivity analysis of RNA down to 100 pg/µL. Includes sample buffer.For 112 samplesFor 112 samplesFor 112 samplesFor 112 samplesRNA SpecificationsAnalytical Specifications R6K ScreenTape High Sensitivity R6K ScreenTapeQuality score RIN e RIN eSensitivity 2 ng/µL 100 pg/µLQuantitative precision 1 15 % CV 20 % CVQualitative range 2–500 ng/µL 100–10,000 pg/µLPhysical SpecificationsAnalysis time16 samples < 20 minutes96 samples ~ 100 minutes16 samples < 15 minutes96 samples ~ 100 minutesSamples per consumable 16 16Sample volume required 1 µL 2 µLKit stability 4 months 4 monthsKit size 112 samples/box 112 samples/box1For the high sensitivity analysis of RNA down to 100 pg/µL.RNA Application NotesPublication Number5990-9613ENDescriptionComparison of RIN and RIN e algorithms for the Agilent 2100 Bioanalyzer and the Agilent 2200 TapeStation systemThis list only provides an overview of selected RNA application notes.Visit our website at www.agilent.com/genomics/tapestation for a complete list of all available application and technical notes.15

Protein SolutionsThe P200 ScreenTape is an automatedsystem for protein QC from 10 to 200 kDaallowing the determination protein size,product purity and the comparison ofdifferent protein profi les.Protein Consumables and ReagentsPart Number Description Quantity5067-5371 P200 ScreenTapeFor the analysis of proteins from 10 to 200 kDa.Includes 7 ScreenTape consumables.5067-5372 P200 ReagentsFor the analysis of proteins from 10 to 200 kDa.Includes ladder, 5X Labeling dye, labeling buffer, reducing and non-reducing sample buffer, pH buffer andmarkers (pre-stained).112 samples112 samplesProtein SpecificationsProtein Application NotesAnalytical SpecificationsP200 ScreenTapePublication NumberDescriptionSizing rangeSizing resolution 1 15 %Sizing accuracySizing precisionSensitivity 2Quantitative rangeQuantitative precisionQualitative rangePhysical SpecificationsAnalysis time10 to 200 kDa± 10% (CAII, lysozyme, BLG)3 % CV5 ng/µL (lysozyme, BSA)12.5 ng/µL (IgG)100–1000 ng/µL (IgG)15 % CV5–5000 ng/µL (BSA, lysozyme)12.5–5000 ng/µL (IgG)16 samples < 15 minutes5990-9052EN5990-9053EN5990-8762EN5990-9603ENReproducible integrity and purity testing ofantibodies with P200 ScreenTapeOptimization and control of protein purifi cationprocedures with P200 ScreenTapeSuppression of antibody reduction artifacts withNEM on P200 ScreenTapeAgilent P200 ScreenTape – Understanding protein,and protein sample buffer effects on staining,denaturation and electrophoresisThis list only provides an overview of selected proteinapplication notes. Visit our website atwww.agilent.com/genomics/tapestationfor a complete list of all available protein applicationand technical notes.Samples per consumable 16Sample volume required 2 µLKit stabilityKit size4 months112 samples1. For ladder2. Signal:noise ratio > 3CAII = carbonic anhydrase, BLG = beta-lactoglobulin, BSA = bovine serum albumin16

Agilent 3100 OFFGEL FractionatorEasy integration into any protein analysis workflowWhether your task is biomarker discovery, protein identifi cation or purifi cation of functionalproteins or peptides, the Agilent 3100 OFFGEL Fractionator fi ts into any protein analysisworkfl ow. Integration of the OFFGEL system is easy and ultimately increases sensitivity ofsubsequent MS detection.Key benefits• pI-based OFFGEL fractionation with liquid-phase recovery for easy transfer todownstream techniques such as LC/MS.• Reproducible fractionation with resolution as low as 0.1 pH for maximum MS sensitivity.• Compatibility with up- or downstream techniques such as immunodepletion, LC/MSor gel-based analysis for maximum fl exibility.• pI values obtained act as additional validation parameters of MS hits and can beused to search for peptides with charged posttranslational modifi cations (PTMs).• All additives can easily be removed after fractionation to avoid interference withnano-electrospray and MS detection.• Fractionation of up to 16 samples in parallel on two trays (eight samples each)for highest throughput.• Broad loading capacity from 50 μg to 5 mg of sample for analytical applicationsor maximum enrichment of low abundance proteins.• OFFGEL mode as well as conventional isoelectric focusing (IEF) based onimmobilized pH gradient (IPG) gels.2D-gel-type analysis of E. coli cell lysatesE. coli cell lysates in the absence and presence of ß-lactoglobuline were labeled andfractionated with the 3100 OFFGEL Fractionator (12-well setup, pH 3-10) and subsequentlyanalyzed using the 2100 Bioanalyzer system. The combination of both systems enables a2D-gel-type analyses with high resolution and high sensitivity, suitable for differential geneexpression applications.Isoelectric point (pl)Molecular weightSize[kDa]10070503015OFFGEL well pHno βLG4.3 4.8 5.3 5.8 6.3 6.7 7.2 7.7 8.2 8.7Size[kDa]10070503015OFFGEL well pH1 % βLG4.3 4.8 5.3 5.8 6.3 6.7 7.2 7.7 8.2 8.75517

Agilent 3100 OFFGEL FractionatorThe Agilent 3100 OFFGEL Fractionatorprovides excellent pI-based fractionationof proteins and peptides with liquidphase recovery prior to LC/MS analysis.It can dramatically increase the numberof proteins ultimately identifi ed.Agilent 3100 OFFGEL Fractionator SystemPart Number Description QuantityG3100AA3100 OFFGEL FractionatorIncludes local controller, preinstalled software and default methods, power cable, two electrode sets, two trays, and optionalinstallation and familiarization service.1 systemAccessories and Spare PartsPart Number Description Quantity5188-8012 12-well-Frame SetTo be used with 13 cm IPG gel strips and respective ampholytes; for the following kits: 5188-6444, 5188-6425, 5188-6427.Includes well frames (12 wells), cover seals, electrode pads, glycerol solution, cover fl uid and manual.5188-8013 24-well-Frame SetTo be used with 24 cm IPG gel strips and respective ampholytes; for the following kits: 5188-6444, 5188-6424, 5188-6426.Includes 12 well frames (24 wells), cover seals, electrode pads, glycerol solution, cover fl uid and manual.G3100-60007 Tray SetIncludes replacement trays.G3100-60002 Electrode AssemblyIncludes 1 fi xed and 1 movable electrode, and 1 tray.12 well frames12 well frames4 trays1 assemblyServicesFor more details on our service portfolio, please contact your sales representative orAgilent’s Worldwide Sales and Support Phone Assistancewww.agilent.com/chem/contactus18

OFFGEL Electrophoresis SolutionsThe Agilent 3100 OFFGEL Fractionatorperforms isoelectric focusing of proteins orpeptides in immobilized pH gradient (IPG)gel strips offering high and low resolutionin different pH ranges.OFFGEL Electrophoresis Kits and ReagentsPart Number Description Quantity5188-6444 Starter KitFractionation of protein or peptide samples.Includes IPG gel strips, well frames, cover seals, paper pads, cover fl uid and reagents for fractionation and in-liquidrecovery: three samples pH 3-10, 12 fractions; three samples pH 3-10, 24 fractions; three samples pH 4-7, 12 fractions;three samples pH 4-7, 24 fractions.5188-6425 Low Resolution Kit, pH 3-10Fractionation of protein or peptide samples into 12 fractions in the pH range 3-10.Includes IPG gel strips, well frames, cover seals, paper pads, cover fluid and reagents for fractionation and in-liquid recovery.5188-6424 High Resolution Kit, pH 3-10Fractionation of protein or peptide samples into 24 fractions in the pH range 3-10.Includes IPG gel strips, well frames, cover seals, paper pads, cover fluid and reagents for fractionation and in-liquid recovery.5188-6427 Low Resolution Kit, pH 4-7Fractionation of protein or peptide samples into 12 fractions in the pH range 4-7.Includes IPG gel strips, well frames, cover seals, paper pads, cover fl uid and reagents components for fractionation andin-liquid recovery.5188-6426 High Resolution Kit, pH 4-7Fractionation of protein or peptide samples into 24 fractions in the pH range 4-7.Includes: IPG gel strips, well frames, cover seals, paper pads, cover fluid and reagents for fractionation and in-liquid recovery.5188-6428 OFFGEL Protein Test SampleCheckout sample for testing the performance of the 3100 OFFGEL Fractionator.Includes a single protein which will focus into only one fraction when used with the low resolution kit, pH 3-10.For 12 samplesFor 12 samplesFor 12 samplesFor 12 samplesFor 12 samples1 test sampleOFFGEL Electrophoresis Application NotesPublication Number5990-6521EN5989-6419EN5989-5814EN5989-5206EN5989-8419ENDescriptionMonitoring antibody charge variants using a combination of Agilent 3100 OFFGEL Fractionation by isoelectric point and high sensitivity proteindetection with the Agilent 2100 BioanalyzerCombining immuno-depletion, protein and peptide OFFGEL electrophoresis for the effi cient fractionation of plasma prior to LC-MS analysisEffi cient protein fractionation and identifi cation with the Agilent 3100 OFFGEL FractionatorEffi cient peptide fractionation and improved protein identifi cation with the Agilent 3100 OFFGEL FractionatorAlternative to 2 gel electrophoresis – OFFGEL electrophoresis combined with high sensitivity on-chip protein detectionThis list only provides an overview of selected OFFGEL application notes. Visit our website at www.agilent.com/chem/offgelfor a complete list of all available application and technical notes.19

Learn morewww.agilent.com/genomics/bioanalyzerwww.agilent.com/genomics/tapestationwww. agilent.com/chem/offgelBuy onlinewww.agilent.com/chem/storeFind an Agilent customer center in your countrywww.agilent.com/chem/contactusU.S. and Canada1-800-227-9770agilent_inquiries@agilent.comEuropeinfo_agilent@agilent.comAsia Pacificinquiry_lsca@agilent.com© Agilent Technolgies, Inc. 2012Published in the USA, March 15, 20125990-9864EN