Abnova Antibody Pairs for Duolink in situ PLA - Tebu-Bio
Abnova Antibody Pairs for Duolink in situ PLA - Tebu-Bio
Abnova Antibody Pairs for Duolink in situ PLA - Tebu-Bio
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<strong>Antibody</strong> <strong>Pairs</strong> <strong>for</strong><br />
<strong>Duol<strong>in</strong>k</strong> <strong>in</strong> <strong>situ</strong> <strong>PLA</strong><br />
<strong>Abnova</strong><br />
<strong>Abnova</strong> Corporation<br />
distributed <strong>in</strong> Europe by tebu-bio<br />
Dual Recognition<br />
<strong>Antibody</strong> Pair <strong>for</strong><br />
Prote<strong>in</strong> Phosphorylation<br />
Prote<strong>in</strong> Phosphorylation Studies<br />
Product Name: Phospho-DNMT1(Y399)<br />
& DNMT1 Dual Recognition <strong>Antibody</strong> Pair<br />
Catalog #: DP0002<br />
The phosphorylation of DNMT1 prote<strong>in</strong> on<br />
Y399 <strong>in</strong> HeLa cell. Each red dot represents<br />
a phosphorylated prote<strong>in</strong>.<br />
<strong>Duol<strong>in</strong>k</strong> ® <strong>in</strong> <strong>situ</strong><br />
<strong>PLA</strong> reagents<br />
Product Name: Phospho-PDGFRB(Y579)<br />
& PDGFRB Dual Recognition <strong>Antibody</strong> Pair<br />
Catalog #: DP0004<br />
The phosphorylation of PDGFRB prote<strong>in</strong> on<br />
Y579 <strong>in</strong> BJ hTert cell stimulated with<br />
PDGF-BB. Each red dot represents a<br />
phosphorylated prote<strong>in</strong>.<br />
A Revolution <strong>in</strong><br />
Prote<strong>in</strong> Detection<br />
A powerful plat<strong>for</strong>m <strong>for</strong> prote<strong>in</strong> phosphorylation and prote<strong>in</strong>-prote<strong>in</strong> <strong>in</strong>teraction studies <strong>in</strong> <strong>situ</strong> is created by<br />
comb<strong>in</strong><strong>in</strong>g <strong>Abnova</strong>’s dual recognition, primary antibody pair with <strong>Duol<strong>in</strong>k</strong> ® <strong>in</strong> <strong>situ</strong> proximity ligation assay (<strong>in</strong><br />
<strong>situ</strong> <strong>PLA</strong>) from Ol<strong>in</strong>k <strong>Bio</strong>science. This revolutionary comb<strong>in</strong>ation enables unprecedented specificity and<br />
sensitivity of prote<strong>in</strong> detection and ������������� <strong>for</strong> high per<strong>for</strong>mance ����������������� and immunohistochemistry<br />
applications. Leverag<strong>in</strong>g on <strong>Abnova</strong>’s manufactur<strong>in</strong>g capability and grow<strong>in</strong>g collection of 30,000+<br />
antibodies, and Ol<strong>in</strong>k <strong>Bio</strong>science <strong>in</strong> <strong>situ</strong> <strong>PLA</strong> technology, hundreds of target ������� and validated assays are<br />
be<strong>in</strong>g developed and marketed by both companies <strong>in</strong> cells and tissue samples.<br />
Pr<strong>in</strong>ciple of <strong>Duol<strong>in</strong>k</strong><br />
1. Incubate with target primary antibodies<br />
from two different species<br />
2. Add <strong>PLA</strong> probes PLUS and MINUS<br />
3. Hybridize connector oligos<br />
4. Ligation to <strong>for</strong>m a complete DNA circle 5. Roll<strong>in</strong>g circle amplification 6. Add fluorescent probes to reveal<br />
phosphorylation<br />
Product Name: Phospho-RAF1(S259)<br />
& RAF1 Dual Recognition <strong>Antibody</strong> Pair<br />
Catalog #: DP0003<br />
The phosphorylation of RAF1 prote<strong>in</strong> on<br />
T269 <strong>in</strong> HeLa cell. Each red dot represents<br />
a phosphorylated prote<strong>in</strong>.<br />
www.tebu-bio.com
TM<br />
<strong>Abnova</strong> Corporation<br />
distributed <strong>in</strong> Europe by tebu-bio<br />
<strong>Antibody</strong> pairs <strong>for</strong> <strong>Duol<strong>in</strong>k</strong> <strong>in</strong> <strong>situ</strong> <strong>PLA</strong><br />
Catalog # Product Name<br />
DP0052 Phospho-BRAF(S445) & BRAF Dual Recognition <strong>Antibody</strong> Pair<br />
DP0042 Phospho-CDK2(T160) & CDK2 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0022 Phospho-CDKN1A(T145) & CDKN1A Dual Recognition <strong>Antibody</strong> Pair<br />
DP0024 Phospho-CDKN1A(T57) & CDKN1A Dual Recognition <strong>Antibody</strong> Pair<br />
DP0017 Phospho-DNMT1(S714) & DNMT1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0002 Phospho-DNMT1(Y399) & DNMT1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0016 Phospho-DNMT1(Y969) & DNMT1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0020 Phospho-EGFR(S1166) & EGFR Dual Recognition <strong>Antibody</strong> Pair<br />
DP0027 Phospho-EGFR(Y1197) & EGFR Dual Recognition <strong>Antibody</strong> Pair<br />
DP0034 Phospho-FGFR1(Y154) & FGFR1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0025 Phospho-FGFR1(Y463) & FGFR1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0043 Phospho-IKBKB(Y199) & IKBKB Dual Recognition <strong>Antibody</strong> Pair<br />
DP0040 Phospho-IKBKG(S31) & IKBKG Dual Recognition <strong>Antibody</strong> Pair<br />
DP0035 Phospho-MAP2K5(S137) & MAP2K5 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0011 Phospho-MAP2K5(S149) & MAP2K5 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0039 Phospho-MAP3K5(S83) & MAP3K5 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0050 Phospho-MAP3K5(S966) & MAP3K5 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0037 Phospho-MAP4K4(S631) & MAP4K4 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0030 Phospho-MCAM(Y641) & MCAM Dual Recognition <strong>Antibody</strong> Pair<br />
DP0021 Phospho-NFKB1(S337) & NFKB1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0023 Phospho-NFKB1(S893) & NFKB1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0036 Phospho-NFKB1(S932) & NFKB1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0053 Phospho-PAK1(T423) & PAK1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0010 Phospho-PDGFRA(Y1018) & PDGFRA Dual Recognition <strong>Antibody</strong> Pair<br />
DP0013 Phospho-PDGFRA(Y572) & PDGFRA Dual Recognition <strong>Antibody</strong> Pair<br />
DP0009 Phospho-PDGFRA(Y574) & PDGFRA Dual Recognition <strong>Antibody</strong> Pair<br />
DP0028 Phospho-PDGFRA(Y754) & PDGFRA Dual Recognition <strong>Antibody</strong> Pair<br />
DP0031 Phospho-PDGFRA(Y988) & PDGFRA Dual Recognition <strong>Antibody</strong> Pair<br />
DP0006 Phospho-PDGFRB(Y1021) & PDGFRB Dual Recognition <strong>Antibody</strong> Pair<br />
DP0004 Phospho-PDGFRB(Y579) & PDGFRB Dual Recognition <strong>Antibody</strong> Pair<br />
DP0005 Phospho-PDGFRB(Y740) & PDGFRB Dual Recognition <strong>Antibody</strong> Pair<br />
DP0054 Phospho-PRL(S163) & PRL Dual Recognition <strong>Antibody</strong> Pair<br />
DP0003 Phospho-RAF1(S259) & RAF1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0019 Phospho-RAF1(S339) & RAF1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0029 Phospho-RAF1(Y340) & RAF1 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0012 Phospho-SMAD3(S208) & SMAD3 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0026 Phospho-SMAD3(S213) & SMAD3 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0015 Phospho-TP53(S37) & TP53 Dual Recognition <strong>Antibody</strong> Pair<br />
DP0014 Phospho-TP53(S9) & TP53 Dual Recognition <strong>Antibody</strong> Pair<br />
To complete the <strong>Duol<strong>in</strong>k</strong> <strong>in</strong> <strong>situ</strong> <strong>PLA</strong> assay <strong>for</strong> above experiment, please order each of the<br />
follow<strong>in</strong>g reagents from Ol<strong>in</strong>k <strong>Bio</strong>science (www.ol<strong>in</strong>k.com):<br />
Catalog # Product Name<br />
Art no. 90202 <strong>Duol<strong>in</strong>k</strong> <strong>PLA</strong> probe Anti-Rabbit MINUS<br />
Art no. 90301 <strong>Duol<strong>in</strong>k</strong> <strong>PLA</strong> probe Anti-Mouse PLUS<br />
Art no. 90103 <strong>Duol<strong>in</strong>k</strong> 100 Detection Kit 613<br />
or Art no. 90104 <strong>Duol<strong>in</strong>k</strong> 100 Detection Kit 563<br />
Art no. 80100 <strong>Duol<strong>in</strong>k</strong> Mount<strong>in</strong>g Medium, 10ml<br />
Visit www.t e b u - b i o .com and ref<strong>in</strong>e: <strong>in</strong> <strong>situ</strong> <strong>PLA</strong><br />
<strong>in</strong> Assays & kits, Simplex Quantification<br />
P<br />
www.tebu-bio.com
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