uric acid LS - Agentúra Harmony vos

uric acidUricase-PAPPREPARATION AND STABILITYCOBAS-MIRARef. 30390, 30391, 30392. Dissolve the contents of one bottle R.2, to the contents of one bottle buffer solution R.1.This working reagent is stable 4 weeks at 2-8ºC or 10 days at room temperature, when stored in a dark bottle.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0.00 mg/dlMEASUREMENT MODE : ABSORB HIGH : 25.00 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 2.50 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 7.00 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 2 READINGS FIRST : CB LAST : 12UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 2CONC. FACTOR: NOBLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPLREAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 25 mg/dL of Uric Acid.3Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

uric acid LSUricase-PAP / Liquid reagentPREPARATION AND STABILITYCOBAS-MIRARef. 30393. The reagent is ready to use.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0.00 mg/dlMEASUREMENT MODE : ABSORB HIGH : 25.00 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 2.50 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 7.00 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 550 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 2 READINGS FIRST : CB LAST : 12UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 2CONC. FACTOR: NOBLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPLREAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 25 mg/dL of Uric Acid.5Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

albuminBromcresol GreenPREPARATION AND STABILITYCOBAS-MIRARef. 30120, 30121. The reagent is ready to be used.This solution is stable at 2-8ºC up to the date of expiration specified.Avoid direct sunlight.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : NO MEASUREMENT MODE : ABSORB HIGH : NO REACTION MODE : R-S NORM. RANGE LOW : NO CALIBRATION MODE : SLOPE AVG HIGH : NO REAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 600 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 2 READINGS FIRST : CB LAST : 8UNIT : g / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * g/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 6 g/dL of Albumin.7Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

α-amylaseCNP-G 3 blocked - Liquid reagentPREPARATION AND STABILITYCOBAS-MIRARef. 30140. Reagent ready to use.Unopened reagent is stable until the expiration date printed on the label when stored at 2-8ºC. After opening, the reagent isstable for 60 days when properly capped immediately after each opening and stored at 2-8ºC.CALIBRATIONCalibration with a factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 5 U/LMEASUREMENT MODE : ABSORB HIGH : 1100 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 90 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 405 nm CALC. STEP A : KINSEARCH DECIMAL POSITION : 0 READINGS FIRST : 2 LAST : 10UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 5.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl FACTOR : 5814CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000LINEARITYThis method is linear up to 2000 U/L of Amylase.9Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

ilirubinDMSO - Total and DirectPREPARATION AND STABILITYCOBAS-MIRARef. 30150, 30151. Total bilirubin.Ref. 30155, 30156. Direct bilirubin.Prepare the monoreagent mixing 30 vol. of R.1 with 1 vol. of Nitrite.The working reagents are stable 48 hours at 2-8ºC.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : 0.0 mg/dlHIGH : 20.0 mg/dlWAVELENGTH : 550 nm NORM. RANGE LOW : 0.0 mg/dlDECIMAL POSITION : 1 HIGH : 1.1 o 0.3 mg/dlUNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 2 LAST : 14SAMPLE CYCLE : 3VOLUME : 15.0 µlDILUTION NAME : H2O VOLUME : 20.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 250 µl BLANKREAG. RANGE LOW : NO HIGH : NO BLANK RANGE LOW : NO HIGH : NO STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 20 mg/dL of Bilirubin.13Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

ilirubinDMSO - Total and DirectPREPARATION AND STABILITYCOBAS-MIRARef. 30150, 30151. Total bilirubin.Ref. 30155, 30156. Direct bilirubin.Both reagents are ready to use.Are stable until the expiration date stated on the label.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : 0.0 mg/dlHIGH : 20.0 mg/dlWAVELENGTH : 550 nm NORM. RANGE LOW : 0.0 mg/dlDECIMAL POSITION : 1 HIGH : 1.1 o 0.3 mg/dlUNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 2 LAST : 14SAMPLE CYCLE : 3VOLUME : 15.0 µlDILUTION NAME : H2O VOLUME : 20.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 250 µl BLANKREAG. RANGE LOW : NO HIGH : NO BLANK RANGE LOW : NO HIGH : NO STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 20 mg/dL of Bilirubin.15Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

calciumarsenazo IIIPREPARATION AND STABILITYCOBAS-MIRARef. 30160, 30161. The monoreagent is ready for use.Avoid direct sunlight and at 2-25ºC is stable up to the date of expiration as specified.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0.0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 30.0 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 9.0 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 11.0 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 600 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : CB LAST : 6UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 2 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 30 mg/dL of Calcium.23Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

calciumO-cresolphtaleinPREPARATION AND STABILITYCOBAS-MIRARef. 30165. All reagents are ready for use.Stored at 2-8ºC up to the date of expiration as specified.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : 0.0 mg/dlHIGH : 30.0 mg/dlWAVELENGTH : 550 nm NORM. RANGE LOW : 9.0 mg/dlDECIMAL POSITION : 1 HIGH : 11.0 mg/dlUNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 1 LAST : 8SAMPLE CYCLE : 2VOLUME : 4.0 µlDILUTION NAME : H2O VOLUME : 15.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 400 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 1 HIGH : NO VOLUME : 10.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 15.0 µlSTANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 16 mg/dL of Calcium.25Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

calciumO-cresolphtalein V/VPREPARATION AND STABILITYCOBAS-MIRARef. 30165. All reagents are ready for use.Stored at 2-8ºC up to the date of expiration as specified.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : NO HIGH : NO WAVELENGTH : 550 nm NORM. RANGE LOW : NO DECIMAL POSITION : 1 HIGH : NO UNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 2 LAST : 8SAMPLE CYCLE : 1VOLUME : 2.0 µlDILUTION NAME : H2O VOLUME : 10.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 100 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 3 HIGH : NO VOLUME : 95.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 0.0 µlSTANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 15.0 mg/dL of Calcium.27Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

HDL cholesterolBursteinPREPARATION AND STABILITYCOBAS-MIRARef. 30188. Precipitating reagent is ready for use.Stored at 2-8ºC is stable up to the date of expiration as specified..Stability 4 months at 2-8 ºC or 15 days at room temperature avoid direct sunlight.CALIBRATIONUse a human serum calibrator (Ref. 30910). Use the value of cholesterol multiplied by 1.1.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 5 mg/dlMEASUREMENT MODE : ABSORB HIGH : 275 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 5 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 65 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 9UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 10.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 350 mg/dL of HDL Cholesterol.29Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

HDL cholesterolDirect - LiquidPREPARATION AND STABILITYCOBAS-MIRARef. 30189. Both reagents are ready to use.Stored at 2-8 ºC are stable up to the date of expiration as specified. Once opened the stability is 2 months at 2-8ºC.CALIBRATIONUse the specific serum included in the kit.The calibrator once reconstituted is stable one week at 2-8ºC or 5 weeks at –20ºC.OPERATORY CONDITIONSIntroduce the following data in the computer:GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : NO HIGH : NO WAVELENGTH : 600 nm NORM. RANGE LOW : NO DECIMAL POSITION : 0 HIGH : NO UNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 11 LAST : 24SAMPLE CYCLE : 1VOLUME : 3.0 µlDILUTION NAME : H2O VOLUME : 10.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 240 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 10 HIGH : NO VOLUME : 80.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 0.0 µlSTANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 200 mg/dL of HDL Cholesterol.31Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

LDL cholesterolDirect - LiquidPREPARATION AND STABILITYCOBAS-MIRARef. 30186. Both reagents are ready to use.Stored at 2-8 ºC are stable up to the date of expiration as specified. Once opened the stability is 2 months at 2-8ºC.CALIBRATIONUse the specific serum included in the kit.The calibrator once reconstituted is stable one week at 2-8ºC or 5 weeks at –20ºC.OPERATORY CONDITIONSIntroduce the following data in the computer:GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : NO HIGH : NO WAVELENGTH : 600 nm NORM. RANGE LOW : NO DECIMAL POSITION : 0 HIGH : NO UNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 11 LAST : 24SAMPLE CYCLE : 1VOLUME : 3.0 µlDILUTION NAME : H2O VOLUME : 10.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 240 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 10 HIGH : NO VOLUME : 80.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 0.0 µlSTANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 1000 mg/dL of LDL Cholesterol.33Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

cholesterolenzymatic CHOD-PAPPREPARATION AND STABILITYCOBAS-MIRARef. 30180, 30181, 30182. Dissolve the contents of one bottle (R.2) to the contents of one bottle Buffer reagent R.1.This working reagent is stable 4 months refrigerated or 15 days at room temperature.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 600 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 120 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 220 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 600 mg/dL of Cholesterol.35Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

Cholesterol LSenzymatic CHOD-PAP / Liquid reagentPREPARATION AND STABILITYCOBAS-MIRARef. 30183, 30184. Reagent ready to use.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 600 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 120 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 220 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 600 mg/dL of Cholesterol.37Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

CK-NACOptimisedPREPARATION AND STABILITYCOBAS-MIRARef. 30200. Dissolve one tablet in 2,5 mL. of buffer R.1.This working reagent is stable: 5 days at 2-8 ºC o 24 hours at room temperature.CALIBRATIONCalibration with a factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 10 U/LMEASUREMENT MODE : ABSORB HIGH : 1500 U/LREACTION MODE : R-S NORM. RANGE LOW : 24 U/LCALIBRATION MODE : FACTOR HIGH : 195 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 6 LAST : 14UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 3.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl FACTOR : 11905CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 1500 U/L of CK.41Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

CK-MBOptimisedPREPARATION AND STABILITYCOBAS-MIRARef. 30203. Dissolve one vial of R.2 with 2,5 mL of buffer R.1.Stable for seven days at 2-8 ºC or 12 hours at 15-25 ºC.Bring to assay temperature before use.CALIBRATIONCalibration in front of a factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 5 U/LMEASUREMENT MODE : ABSORB HIGH : 1000 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 24 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 1 READINGS FIRST : 16 LAST : 26UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl FACTOR : 5185CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 1000 U/L of CK.45Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

creatinineJafféPREPARATION AND STABILITYCOBAS-MIRARef. 30210, 30211. Reagent mixture: Mix proportionally 1:1 the reagents R.1 and R.2.Stability: 10 days at room temperature.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 15 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 0.7 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 1.4 mg/dlREAGENT BLANK : REAG / DIL CLEANER : BEFORE NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : 2 LAST : 5UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 2.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 15 mg/dL of Creatinine.47Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

cholinesteraseButyrylthiocholinePREPARATION AND STABILITYCOBAS-MIRARef. 30190. Dissolve one tablet in 3 ml. of buffer R.1.This working reagent is stable 2 hours at 2-8 ºC.CALIBRATIONCalibration in front of a Factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 500 U/LMEASUREMENT MODE : ABSORB HIGH : 12500 U/LREACTION MODE : R-S NORM. RANGE LOW : 4659 U/LCALIBRATION MODE : FACTOR HIGH : 14443 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 405 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 2 LAST : 10UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 3.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 2.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl FACTOR : 22710CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 12500 U/L of Cholinesterase.49Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

copperColour 3-5 Di Br-PAESAPREPARATION AND STABILITYCOBAS-MIRARef. 30205. Solution R.1+R.3: Add one small spoon of R.3 into one vial of R1. Mix. This is Reagent 1 in the analyser.Stability: 15 days at 2-8ºC. The R.2 is ready for use.CALIBRATIONUse a human serum calibrator (Ref. 30910) or the standard included in the kit.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 µg / dlMEASUREMENT MODE : ABSORB HIGH : 500 µg / dlREACTION MODE : R-S-SR1 NORM. RANGE LOW : 70 µg / dlCALIBRATION MODE : SLOPE AVG HIGH : 155 µg / dlREAGENT BLANK : REAG / DIL CLEANER : BEFORE NUMBER OF STEPS : 1WAVELENGTH : 600 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : 2 LAST : 13UNIT : µg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 10.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * µg / dlSTART R1 CYCLE : 3 STD-2 : NOVOLUME : 10 µL STD-3 : NODILUTION VOLUME : H2O REPLICATE : DUPL VOLUME : 10 µL DEVIATION : 10.0 %CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 500 µg/dL of Copper.53Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

copperColour 3-5 Di Br-PAESAPREPARATION AND STABILITYCOBAS-MIRARef. 30205. Solution R.1+R.3: Add one small spoon of R.3 into one vial of R1. Mix. This is Reagent 1 in the analyser.Stability: 15 days at 2-8ºC. The R.2 is ready for use.CALIBRATIONUse a human serum calibrator (Ref. 30910) or the standard included in the kit.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 µg / dlMEASUREMENT MODE : ABSORB HIGH : 500 µg / dlREACTION MODE : R-S-SR1 NORM. RANGE LOW : 70 µg / dlCALIBRATION MODE : SLOPE AVG HIGH : 155 µg / dlREAGENT BLANK : REAG / DIL CLEANER : BEFORE NUMBER OF STEPS : 1WAVELENGTH : 600 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : 2 LAST : 13UNIT : µg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 10.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * µg / dlSTART R1 CYCLE : 3 STD-2 : NOVOLUME : 10 µL STD-3 : NODILUTION VOLUME : H2O REPLICATE : DUPL VOLUME : 10 µL DEVIATION : 10.0 %CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 500 µg/dL of Copper.55Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

acid phosphataseHillman method modif.PREPARATION AND STABILITYCOBAS-MIRARef. 30110. Dissolve one tablet in 2 mL. of buffer R.1.The stability of working reagent is 2 days at 2-8ºC or 6 hours at room temperature.CALIBRATIONCalibration in front of a Factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 U/LMEASUREMENT MODE : ABSORB HIGH : 150 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 5.4 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 405 nm CALC. STEP A : KINSEARCH DECIMAL POSITION : 1 READINGS FIRST : 12 LAST : 20UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 0.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl FACTOR : 1440CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 150 U/L of Acid phosphatase.57Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

alkaline phosphataseMethod DGKCPREPARATION AND STABILITYCOBAS-MIRARef. 30130. Dissolve one tablet in 3 mL. of buffer R.1.Ref. 30131. Dissolve one tablet with 15 mL of buffer R.1.The working reagent is stable 5 days at 15-25 ºC or 21 days at 2-8ºC.CALIBRATIONCalibration with a factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 U/LMEASUREMENT MODE : ABSORB HIGH : 1200 U/LREACTION MODE : R-S NORM. RANGE LOW : 98 U/LCALIBRATION MODE : FACTOR HIGH : 279 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 405 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 2 LAST : 12UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 3.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 20 µl FACTOR : 3989CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 1200 U/L of Alkaline phosphatase.61Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

phosphorusU.VPREPARATION AND STABILITYCOBAS-MIRARef. 30330. The reagent is ready for use.It’s stable at 2-8ºC up to the date of expiration as specified.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0.5 mg/dlMEASUREMENT MODE : ABSORB HIGH : 20 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 1.5 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 7.0 mg/dlREAGENT BLANK : REAG / DIL CLEANER : BEFORE NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : CB LAST : 6UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 20 mg/dL of Phosphorus.67Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

phospholipidsCholine-PAPCOBAS-MIRAPREPARATION AND STABILITYRef. 30320. Dissolve the contents of one bottle R.2 with 10 mL Buffer reagent R.1.Stability: 3 weeks at 2-8ºC or 7 days at 15-25 ºC avoid direct sunlight.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 20 mg/dlMEASUREMENT MODE : ABSORB HIGH : 600 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 160 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 270 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 20 mg/dL of Phospholipids.69Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

GOT / ASTIFCC optimisedPREPARATION AND STABILITYCOBAS-MIRARef. 30240. Dissolve one tablet in 2 mL. of buffer R.1.Ref. 30241. Dissolve one tablet with 15 mL of buffer R.1.Ref. 30242. Dissolve one tablet in one vial of 50 mL of buffer R.1.The working reagent is stable 72 hours at 18-25ºC or 21 days at 2-8ºC.CALIBRATIONCalibration in front of a Factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 U/LMEASUREMENT MODE : ABSORB HIGH : 450 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 38 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINSEARCH DECIMAL POSITION : 0 READINGS FIRST : 4 LAST : 14UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 5.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 180 µl FACTOR : 3042CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : DECREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 450 U/L of GOT.73Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

GPT/ALTIFCC optimisedPREPARATION AND STABILITYCOBAS-MIRARef. 30250. Dissolve one tablet in 2 mL. of buffer R.1.Ref. 30251. Dissolve one tablet with 15 mL of buffer R.1.Ref. 30252. Dissolve one tablet in one vial of 50 mL of buffer R.1.The monoreagent is stable 72 hours at 15-25ºC or 21 days at 2-8ºC.CALIBRATIONCalibration in front of one Factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 U/LMEASUREMENT MODE : ABSORB HIGH : 450 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 40 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 4 LAST : 14UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 5.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 180 µl FACTOR : 3042CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : DECREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 450 U/L of GPT/ALT.81Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

γ-GTCarboxy substratePREPARATION AND STABILITYCOBAS-MIRARef. 30220. Dissolve one tablet in 2 mL. buffer R.1.Ref. 30221. Dissolve one tablet with 15 mL of buffer R.1.The monoreagent is stable 5 days at 15-25ºC or 21 days at 2-8ºC.CALIBRATIONCalibration in front of a Factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 U/LMEASUREMENT MODE : ABSORB HIGH : 250 U/LREACTION MODE : R-S NORM. RANGE LOW : 7 U/LCALIBRATION MODE : FACTOR HIGH : 50 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 405 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 3 LAST : 11UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 2.0CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl FACTOR : 2264CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : INCREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 400 U/L of γ-GT.89Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

glucoseHexokinasePREPARATION AND STABILITYCOBAS-MIRARef. 30236. Dissolve the contents of one bottle R.2 to the contents of one bottle Buffer solution R.1.This working reagent is stable 30 days at 2-8 ºC or 7 days at 15-25 ºC. Avoid direct sunlight.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 30 mg/dlMEASUREMENT MODE : ABSORB HIGH : 800 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 60 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 110 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT: mg / dlCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 800 mg/dL of Glucose.95Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

glucoseenzymatic GOD-PAPPREPARATION AND STABILITYCOBAS-MIRARef. 30231, 30232. Dissolve the contents of one bottle R.2 to the contents of one bottle Buffer Solution R.1.This working reagent is stable 1 month at 2-8ºC or 7 days at room temperature.CALIBRATIONUse a human serum calibrator (Ref. 30910).OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 500 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 55 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 110 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 500 mg/dL of Glucosa.97Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

glucoseenzymatic GOD-PAP - Liquid reagentPREPARATION AND STABILITYCOBAS-MIRARef. 30233, 30234. The reagent is ready to use.CALIBRATIONUse a human serum calibrator (Ref. 30910).OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 500 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 55 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 110 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 500 mg/dL of Glucosa.99Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

ironFerrozinePREPARATION AND STABILITYCOBAS-MIRARef. 30270. Dissolve the contents of one tube R.2 in one bottle of buffer R.1.The reagent solution R.1+R.2 is stable 3 months at 2-8ºC or 1 month at room temperature.The R.3 is ready to be use like reagent 2 in the analyser.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : BEFORE TEST RANGE LOW : 0 µg / dlHIGH : 1000 µg / dlWAVELENGTH : 550 nm NORM. RANGE LOW : 37 µg / dlDECIMAL POSITION : 0 HIGH : 160 µg / dlUNIT : µg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 4 LAST : 15SAMPLE CYCLE : 1VOLUME : 35.0 µlDILUTION NAME : H2O VOLUME : 35.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 170 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 5 HIGH : NO VOLUME : 7.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 15.0 µlSTANDARD POS : 1STD-1 : * µg / dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 700 µg/dL of Iron.103Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

lactateEnzymatic-PAPPREPARATION AND STABILITYCOBAS-MIRARef. 30280. Dissolve one vial of enzimas R.2, with 50 mL of buffer R.1.This working reagent is stable 4 weeks at 2-8ºC or 1 week at 15-25ºC.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 150 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 9 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 16 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : CB LAST : 8UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 150 mg/dL of Lactate.111Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

LDHoptimisedPREPARATION AND STABILITYCOBAS-MIRARef. 30290. Dissolve one tablet in 3 mL. of buffer R.1.Ref. 30291. Dissolve one tablet with 15 mL of buffer R.1.The working reagent is stable 5 days at 2-8ºC or 24 hours at room temperature.CALIBRATIONCalibration with a factor.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 10 U/LMEASUREMENT MODE : ABSORB HIGH : 1500 U/LREACTION MODE : R-S NORM. RANGE LOW : 0 U/LCALIBRATION MODE : FACTOR HIGH : 475 U/LREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINSEARCHDECIMAL POSITION : 0 READINGS FIRST : 2 LAST : 10UNIT : U / L REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR : 5.00CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 200 µl FACTOR : 11905CALCULATIONSAMPLE LIMIT: NO REAC. DIRECTION : DECREASE CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 1500 U/L of LDH.113Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

lipaseenzymatic liquidPREPARATION AND STABILITYCOBAS-MIRARef. 30297. Both reagents are ready to use.CALIBRATIONStandard: reconstitute the contents of one vial with 1 mL of distilled water. Mix gently until complete solution.Stability: 10 days at 2-8ºC.Aliquote calibrator solution into small volumes and freeze.Stability:3 months at –20ºC.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : SELECT TEST RANGE LOW : 0 U / l( AFTER TRIG ) HIGH : 250 U / lWAVELENGTH : 600 nm NORM. RANGE LOW : 0 U / lDECIMAL POSITION : 0 HIGH : 30 U / lUNIT : U / l NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : KINETIC READINGS FIRST : 4 LAST : 10SAMPLE CYCLE : 2 REACTION LIMIT : NOVOLUME : 3.0 µlDILUTION NAME : H2O VOLUME : 20.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 250 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 1 HIGH : NO VOLUME : 20.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 20.0 µlSTANDARD POS : 1STD-1 : * U / lCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 250 U/L of Lipase.123Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

magnesiumCalmagitePREPARATION AND STABILITYCOBAS-MIRARef. 30300. Mix 1 volume of buffer solution (R.1) with 1 volume of calmagite solution (R.2).Stability: 24 hours at 15-25ºC4 days at 2-8ºC.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0.0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 5.0 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 1.6 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 2.5 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : 2 LAST : 10UNIT: mg / dlCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 3 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 5 mg/dL of Magnesium.125Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

total proteinsBiuretPREPARATION AND STABILITYCOBAS-MIRARef. 30350, 30351. The reagent is ready for use.It’s stable up to the date of expiration as specified.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 1.0 g/dlMEASUREMENT MODE : ABSORB HIGH : 15.0 g/dlREACTION MODE : R-S NORM. RANGE LOW : 6.7 g/dlCALIBRATION MODE : SLOPE AVG HIGH : 8.7 g/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 550 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READINGS FIRST : CB LAST : 8UNIT : g / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 250 µl STANDARD POS : 1STD-1 : * g/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 15 g/dL of Total Protein.129Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

total proteinsin urine and CSFPyrogallol RedPREPARATION AND STABILITYCOBAS-MIRARef. 30310. The reagent is ready for use.It’s stable up to the date of expiration as specified.Avoid direct sunlight.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 125 mg/lMEASUREMENT MODE : ABSORB HIGH : 4000 mg/lREACTION MODE : R-S NORM. RANGE LOW : 0 mg/lCALIBRATION MODE : SLOPE AVG HIGH : 100 mg/lREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 600 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 2 READINGS FIRST : CB LAST : 8UNIT: mg / lCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR : 3.00BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 25.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/lCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 4 g/L of Proteins.131Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

triglyceridesEnzymatic-PAPPREPARATION AND STABILITYCOBAS-MIRARef. 30360, 30361, 30362, 30363. Dissolve the contents of one vial of enzimas (R.2) to the contents of one bottle of buffer(R.1).This working reagent is stable 1 month at 2-8ºC or 1 week at room temperature.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 700 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 36 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 150 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 700 mg/dL of Triglycerides.133Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

triglycerides LSEnzymatic-PAP - Liquid reagentPREPARATION AND STABILITYCOBAS-MIRARef. 30364, 30365. Reagent ready to use.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 1000 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 36 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 150 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 500 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 0 READINGS FIRST : CB LAST : 10UNIT : mg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 4.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 30.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 1000 mg/dL of Triglycerides.135Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

ureaUrease GLDHPREPARATION AND STABILITYCOBAS-MIRARef. 30370, 30371. Dissolve the contents of one vial of R.2 in one bottle of buffer R.1.The working reagent is stable 6 weeks at 2-8ºC or 7 days at 15-25ºC.CALIBRATIONUse a human serum calibrator.OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 mg/dlMEASUREMENT MODE : ABSORB HIGH : 500 mg/dlREACTION MODE : R-S NORM. RANGE LOW : 15 mg/dlCALIBRATION MODE : SLOPE AVG HIGH : 45 mg/dlREAGENT BLANK : REAG / DIL CLEANER : NO NUMBER OF STEPS : 1WAVELENGTH : 340 nm CALC. STEP A : KINETIC DECIMAL POSITION : 0 READING FIRST : 2 LAST : 5UNIT : mg / dl REACTION LIMIT : NOCALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 3.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 5.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * mg/dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : DECREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of controls and standards.LINEARITYThis method is linear up to 400 mg/dL of Urea.137Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

ureaBerthelotPREPARATION AND STABILITYCOBAS-MIRARef. 30380, 30381. Dissolve one tablet R.3 in one vial of buffer R.1.The stability of this solution is 4 weeks at 2-8 ºC or 1 week at 20-25ºC.The reagent R.2 is ready for use.CALIBRATIONUse a human serum calibrator (Ref. 30910)OPERATORY CONDITIONSIntroduce the following data in the computer :GENERALMEASUREMENT MODE : ABSORB CONVERS. FACTOR : 1.00000REACTION MODE : R-S-SR1 OFFSET : 0.00000CALIBRATION MODE : SLOPE AVG REAGENT BLANK : REAG / DIL CLEANER : NO TEST RANGE LOW : 0 mg / dlHIGH : 200 mg / dlWAVELENGTH : 600 nm NORM. RANGE LOW : 15 mg / dlDECIMAL POSITION : 1 HIGH : 45 mg / dlUNIT : mg / dl NUMBER OF STEPS : 1ANALYSISPOST DIL. FACTOR: NO CONC. FACTOR : NO CALC. STEP A : ENDPOINT READINGS FIRST : 13 LAST : 25SAMPLE CYCLE : 1VOLUME : 2.0 µlDILUTION NAME : H2O VOLUME : 10.0 µl CALIBRATIONCALIB. INTERVAL : ON REQUEST REAGENT CYCLE : 1VOLUME : 100 µl BLANKREAG. RANGE LOW : NO START R1 CYCLE : 12 HIGH : NO VOLUME : 90.0 µl BLANK RANGE LOW : NO DILUTION NAME : H2O HIGH : NO VOLUME : 5.0 µlSTANDARD POS : 1STD-1 : * mg / dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON * Assign the values of controls and standards.LINEARITYThis method is linear up to 200 mg/dL of Urea.141Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es

zincColour 5 Br-PAPSPREPARATION AND STABILITYCOBAS-MIRARef. 30400. Add one small spoon of Reagent 3 into one vial of Reagent 1, and mix.Stability: 30 days at 2-8ºC.Working solution: Mix 10 volumes of solution R1+R3 with one volume of reagent R2.Stability: 10 days at 2-8ºC.CALIBRATIONUse a human serum calibrator (Ref. 30910).OPERATORY CONDITIONSIntroduce the following data in the computer :GENERAL TEST RANGE LOW : 0 µg / dlMEASUREMENT MODE : ABSORB HIGH : 2000 µg / dlREACTION MODE : R-S NORM. RANGE LOW : 68 µg / dlCALIBRATION MODE : SLOPE AVG HIGH : 107 µg / dlREAGENT BLANK : REAG / DIL CLEANER : BEFORE NUMBER OF STEPS : 1WAVELENGTH : 550 nm CALC. STEP A : ENDPOINT DECIMAL POSITION : 1 READING FIRST : CB LAST : 3UNIT : µg / dl CALIBRATIONANALYSIS CALIB. INTERVAL : ON REQUEST POST DIL. FACTOR: NO CONC. FACTOR: NO BLANKSAMPLE CYCLE : 1 REAG. RANGE LOW : NO VOLUME : 15.0 µl HIGH : NO DILUTION NAME : H2O BLANK RANGE LOW : NO VOLUME : 20.0 µl HIGH : NO REAGENT CYCLE : 1VOLUME : 300 µl STANDARD POS : 1STD-1 : * µg / dlCALCULATION STD-2 : NOSAMPLE LIMIT : NO STD-3 : NOREPLICATE : DUPL REAC. DIRECTION : INCREASE DEVIATION : 10.0 %CHECK : ON CONVERS. FACTOR : 1.00000OFFSET : 0.00000* Assign the values of standards.LINEARITYThis method is linear up to 2000 µg/dL of Zinc.143Ed.2002CHEMELEX, S.A. Pol. Ind. Can Castells. C/ Industria 113 nave J 08420 CANOVELLES - SPAINE-Mail: chemelex@terra.es