Spring Bioscience Research Catalogue 2012 - Brunschwig-ch.com

TABLE OF CONTENTSTable of ContentsPrimary Antibody Products 11Detection and Ancillary Products 109Antibody Microarray Products 113Recombinant Protein Products 115Product Index 117

Rabbit Monoclonal Antibodies by Tissue Focus6RootCat# Product Name CloneBreastCardiovascularCervixColorectumEndocrineHematopoietic CellsKidneyLiverM424 Actin-beta SP124 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M308 ALK/p80 SP8 √ √M407 Androgen Receptor (AR) SP107 √ √ √ √ √ √ √ √M347 BAX SP47 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M366 BCL-2 alpha SP66 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M392 BOB-1 SP92 √M391 C4d SP91 EC DCM364 Cadherin-E/E-Cadherin SP64 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M390 Cadherin-N/N-Cadherin SP90 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M317 Calcitonin SP17 √M381 Calpain SP81 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M382 Calpastatin SP82 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M313 Calretinin SP13 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M406 Carbonic Anhydrase 9SP106√(CA-9)M343 Caveolin-1 SP43 √ √ √ √ √ √ √M307 CD3 SP7 TCM335 CD4 SP35 TCM319 CD5 SP19 TCM394 CD7 SP94 TCM316 CD8 SP16 TCM367 CD10 SP67 √ √ √ √M410 CD19 SP110 BCM332 CD20 SP32 BCM404 CD22 SP104 BCM323 CD23 SP23 BCM338 CD31 SP38 EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC EC ECM355 CD43 SP55 TCM337 CD44 Std SP37 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M318 CD79a SP18 BCM419 CD99 SP119 √ √ √ √ √ √ √ √M326 CD117/c-kit SP26 √ √ √ √ √ √ √ √M396 CD163 SP96 √ √M398 CD278/ICOS SP98 √ TC √ √M380 CDK2 SP80 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M354 CDX2 SP54 √ √ √M303 c-erbB-2/HER-2 SP3 √ √ √ √ √ √ √ √ √ √ √ √ √ √M371 c-erbB-3/HER-3 SP71 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M312 Chromogranin A SP12 NE NE NE NE NE NE NE NE NEM344 c-Met SP44 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M359 c-Met (Paired 1003) SP59 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M358 c-Met (Phospho T1003) SP58 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M321 COX-2 SP21 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M383 c-TEN SP83 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M304 Cyclin D1 SP4 PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLCM331 DOG-1 SP31 √ √ √M309 Epidermal Growth Factor SP9 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √Receptor (EGFR)M384 Epidermal Growth Factor SP84 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √Receptor (EGFR)M368 ERCC1 SP68 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M301 Estrogen Receptor (ER) SP1 √ √ √M415 F4/80 SP115 M M K M M M MM423 FLK-1 (mouse) SP123 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M388 FOXA1 SP88 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √LungNervous TissueOsseous TissueOvaryPancreasPlacentaPleuraProstateSkinSM IntestineSoft TissueStomachTestisThyroidUrinary Bladder

RootCat# Product Name CloneBreastCardiovascularCervixColorectumEndocrineHematopoietic CellsKidneyLiverM397 FOXP3 SP97 √ √ √M378 Glial Fibrillary AcidicSP78√Protein (GFAP)M386 Glypican-3 SP86 √ √ √ √ √M420 hENT1 SP120 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M408 IKAROS SP108 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M405 J-chain SP105 PCM327 Keratin 5 SP27 √ √ √ √ √ √ √ √ √ √ √ √ √ √M387 Keratin 6 SP87 √ √ √ √ √ √ √ √ √ √ √ √ √ √M352 Keratin 7 SP52 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M402 Keratin 8 SP102 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M399 Keratin 10 SP99 √ √ √ √ √ √ √ √ √M353 Keratin 14 SP53 √ √ √ √ √ √ √ √ √ √ √ √M395 Keratin 17 SP95 √ √ √ √ √ √ √ √ √ √ √M369 Keratin 18 SP69 √ √ √ √ √ √ √ √ √ √ √ √ √M333 Keratin 20 SP33 √ √ √ √ √M306 Ki-67 SP6 PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLC PLCM351 LMO2 SP51 √M385 MCM2 SP85 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M374 Mesothelin SP74 √ √ √ √ √ √ √M346 MSH2 SP46 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M393 MSH6 SP93 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M414 MUM1/IRF4 SP114 √M372 Myeloperoxidase (MPO) SP72 √M403 Nestin SP103 ST ST STM426 Nitric Oxide Synthase-SP126 M M M M M MInducible (iNOS)M363 p120 (catenin delta 1) SP63 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M305 p53 SP5 √ √ √ √ √M418 p57 SP118 √M334 PAX-5 SP34 √ √ √ √M362 PI3K p85 SP62 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M315 PLAP SP15 √M302 Progesterone Receptor (PR) SP2 √ √ √ √ √ √ √ √ √ √ √M342 Progesterone Receptor (PR) SP42 √ √ √ √ √ √ √ √ √ √ √M329 PSMA SP29 √M350 S6 RibosomalSP50 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √(Phospho S235/236)M345 S6 RibosomalSP45 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √(Phospho S240/244)M422 SLIT2 SP122 √ √ √ √M376 SOX-2 SP76 ST ST ST ST ST ST ST ST ST STM349 Stathmin SP49 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M379 Survivin SP79 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M311 Synaptophysin SP11 NE NE NE NE NE NE NE NEM336 Syntaxin 1A SP36 √ √ √ √ √M370 Tau (Neuronal Marker) SP70 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M412 Thymidylate Synthase (TS) SP112 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M373 Uroplakin III SP73 √ √ √ √ √M328 Vascular EndothelialSP28 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √Growth Factor (VEGF)M320 Vimentin SP20 √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √ √M417 Vinculin SP117 MC,ECMC,ECMC,ECMC,ECMC,ECMC,ECMC,ECMC,ECLungMC,ECNervous TissueMC,ECOsseous TissueMC,ECOvaryMC,ECPancreasMC,ECPlacentaMC,ECPleuraMC,ECProstateMC,ECSkinMC,ECSM IntestineMC,ECSoft TissueMC,ECStomachMC,ECTestisMC,ECThyroidMC,ECUrinary BladderMC,EC√ Indicates the organs or tumors derived from these organs express these markers using the information available in the public domain.Abbreviation: TC = T lymphocyte, BC = B lymphocyte, DC=dendritic cell, EC =endothelial cell, PC= plasma cell, NE = neuroendocrine cell, PLC =proliferating cell, ST=stem cell, M=macrophages,K=Kupffer cells, MC=Cardiac, skeletal, and smooth cells.7

Rabbit Monoclonal Antibodies by Major Research InterestRootCat# Product Name CloneAKT/PKB SignalingAngiogenesisApoptosisB Cell MarkersCancer MarkersCell AdhesionCell CycleCell JunctionsComplementCytoskeletal ProteinsDNA Replication and RepairDNA SynthesisEGFR FamilyEnzymesHematopoiesisHomeodomain ProteinsHormonesImmunoglobulinLymphocyte SignalingNeuroscienceNitrogen MetabolismOncogenesisSignal TransductionStem CellSteroid ReceptorsSynthesis and DegradationTranscriptional RegulatorsTranslational ControlM424 Actin-beta SP124 √M308 ALK/p80 SP8 √M407 Androgen Receptor (AR) SP107 √M347 BAX SP47 √M366 BCL-2 alpha SP66 √M392 BOB-1 SP92 √M391 C4d SP91 √M364 Cadherin-E/E-Cadherin SP64 √M390 Cadherin-N/N-Cadherin SP90 √M317 Calcitonin SP17 √M381 Calpain SP81 √M382 Calpastatin SP82 √M313 Calretinin SP13 √M406 Carbonic Anhydrase 9SP106√(CA-9)M343 Caveolin-1 SP43 √M307 CD3 SP7 √M335 CD4 SP35 √M319 CD5 SP19 √M394 CD7 SP94 √M316 CD8 SP16 √M367 CD10 SP67 √M410 CD19 SP110 √M332 CD20 SP32 √M404 CD22 SP104 √M323 CD23 SP23 √M338 CD31 SP38 √M355 CD43 SP55 √M337 CD44 Std SP37 √M318 CD79a SP18 √M419 CD99 SP119 √M326 CD117/c-kit SP26 √M396 CD163 SP96 √M398 CD278/ICOS SP98 √M380 CDK2 SP80 √M354 CDX2 SP54 √M303 c-erbB-2/HER-2 SP3 √M371 c-erbB-3/HER-3 SP71 √M312 Chromogranin A SP12 √M344 c-Met SP44 √M359 c-Met (Paired 1003) SP59 √M358 c-Met (phospho T1003) SP58 √M321 COX-2 SP21 √M383 c-TEN SP83 √M304 Cyclin D1 SP4 √M331 DOG-1 SP31 √M309 Epidermal Growth Factor SP9√Receptor (EGFR)M384 Epidermal Growth Factor SP84√Receptor (EGFR)M368 ERCC1 SP68 √M301 Estrogen Receptor (ER) SP1 √M415 F4/80 SP115 √8

RootCat# Product Name CloneAKT/PKB SignalingAngiogenesisApoptosisB Cell MarkersCancer MarkersCell AdhesionCell CycleCell JunctionsComplementCytoskeletal ProteinsDNA Replication and RepairDNA SynthesisEGFR FamilyEnzymesHematopoiesisHomeodomain ProteinsHormonesImmunoglobulinLymphocyte SignalingNeuroscienceNitrogen MetabolismOncogenesisSignal TransductionStem CellSteroid ReceptorsSynthesis and DegradationTranscriptional RegulatorsTranslational ControlM423 FLK-1 (mouse) SP123 √M388 FOXA1 SP88 √M397 FOXP3 SP97 √M378 Glial Fibrillary AcidicSP78√Protein (GFAP)M386 Glypican-3 SP86 √M420 hENT1 SP120 √M408 IKAROS SP108 √M405 J-chain SP105 √M327 Keratin 5 SP27 √M387 Keratin 6 SP87 √M352 Keratin 7 SP52 √M402 Keratin 8 SP102 √M399 Keratin 10 SP99 √M353 Keratin 14 SP53 √M395 Keratin 17 SP95 √M369 Keratin 18 SP69 √M333 Keratin 20 SP33 √M306 Ki-67 SP6 √M351 LMO2 SP51 √M385 MCM2 SP85 √M374 Mesothelin SP74 √M346 MSH2 SP46 √M393 MSH6 SP93 √M414 MUM1/IRF4 SP114 √M372 Myeloperoxidase (MPO) SP72 √M403 Nestin SP103 √M426 Nitric Oxide Synthase-SP126√Inducible (iNOS)M363 p120 (catenin delta 1) SP63 √M305 p53 SP5 √M418 p57 SP118 √M334 PAX-5 SP34 √M362 PI3k p85 SP62 √M315 PLAP SP15 √M302 Progesterone Receptor (PR) SP2 √M342 Progesterone Receptor (PR) SP42 √M329 PSMA SP29 √M350 S6 Ribosomal(phospho S235/236)SP50M345 S6 RibosomalSP45(phospho S240/244)M422 SLIT2 SP122 √M376 SOX-2 SP76 √M349 Stathmin SP49 √M379 Survivin SP79 √M311 Synaptophysin SP11 √M336 Syntaxin 1A SP36 √M370 Tau (Neuronal Marker) SP70 √M412 Thymidylate Synthase (TS) SP112 √M373 Uroplakin III SP73 √M328 Vascular EndothelialSP28√Growth Factor (VEGF)M320 Vimentin SP20 √M417 Vinculin SP117 √√√9

FEATURING SP CLONESRabbit MonoclonalDelivering Best-in-ClassRabbit Monoclonals since 2001.CD19Rabbit MonoclonalSP = Spring Bioscience CorporationWhile you may not be familiar with Spring Bioscience Corporation, you are probablyquite familiar with rabbit monoclonal “SP” clones. All SP antibody clone products wereCD19designed and manufactured by Spring Bioscience. It all started Rabbit Monoclonal with SP1, the now goldstandard Estrogen Receptor marker. SP clones continue to emerge as best-in-classimmunohistochemistry markers for their high affinity, sensitivity, and specificity.10Terms and ConditionsSpring Bioscience products are for research use only. They are not for use in diagnostic procedures.Spring Bioscience strives to provide complete and accurate information; however, Spring Bioscience and its business associates assume no liability for any reliance on any of theinformation provided. Furthermore, Spring Bioscience assumes no liability for any omissions. It is the sole responsibility of researchers to independently validate the information and properuse of any products. Spring Bioscience reserves the right to make any changes, additions, and/or deletions to its product information at anytime without notice.

PRIMARY ANTIBODY PRODUCTSSpring Bioscience develops and manufactures awide range of products primarily specialized for IHC.Primary Antibodies – specifically characterizedfor use in IHC, featuring:• Clean and intense staining• Lower pre-treatment requirement• Robust performance for FFPE tissue• Improved reproducibility• Accepted by industry leadersAvailable in 0.1ml, 0.5ml and 1.0ml concentrated sizesas well as a 7ml ready-to-use configuration.Antibody product technologies include:• Rabbit Monoclonal (SP clones)• Mouse Monoclonal (SPM clones)• Rabbit Polyclonal11

Primary AntibodiesActin-Smooth Muscle Rabbit PolyclonalCatalog No. Size FormatE2460 0.1ml ConcentrateE2462 0.5ml ConcentrateE2464 1.0ml ConcentrateE2461 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman alpha smooth muscle isoform of actin.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Leiomyoma, Smooth Musclein Small IntestineCellular Localization: CytoplasmAdrenocorticotrophic Hormone (ACTH) Rabbit PolyclonalCatalog No. Size FormatE3490 0.1ml ConcentrateE3492 0.5ml ConcentrateE3494 1.0ml ConcentrateE3491 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human ACTH.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PituitaryCellular Localization: CytoplasmHuman Smooth Muscle in Small Intestine stained withanti-Actin-Smooth Muscle antibody E246Human Pituitary stained withanti-Adrenocorticotrophic Hormone (ACTH) antibody E349Product Description: This antibody stains smooth muscle cells in vessel walls, gut wall,and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Itreacts with tumors arising from smooth muscles and myoepithelial cells. Actin fromfibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), andmyocardium (alpha-myocardial) are not stained.Product Description: ACTH labels corticotrophs in the adenohypophysis and is useful inthe classification of pituitary adenomas.Actin-Smooth Muscle Mouse MonoclonalCatalog No. Size FormatE14340 0.1ml ConcentrateE14342 0.5ml ConcentrateE14344 1.0ml ConcentrateE14341 7.0ml Ready-to-useClone: 1A4Immunogen: Synthetic peptide from N-terminus ofhuman alpha smooth muscle isoform of actin.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Leiomyoma, Smooth MuscleCellular Localization: CytoplasmAdrenocorticotrophic Hormone (ACTH) Mouse MonoclonalCatalog No. Size Format Clone: SPM501E16450 0.1ml Concentrate Immunogen: ACTH synthetic peptide.E16452 0.5ml Concentrate Application: IHC-PE16454 1.0ml ConcentrateSpecies Reactivity: Human*E16451 7.0ml Ready-to-useIsotype: Mouse IgG1Control Tissue: PituitaryCellular Localization: CytoplasmHuman Smooth Muscle stained withanti-Actin-Smooth Muscle antibody E1434Human Pituitary stained withanti-Adrenocorticotrophic Hormone (ACTH) antibody E1645Product Description: This antibody stains smooth muscle cells in vessel walls, gut wall,and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Itreacts with tumors arising from smooth muscles and myoepithelial cells. Actin fromfibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), andmyocardium (alpha-myocardial) are not stained.Product Description: ACTH labels corticotrophs in the adenohypophysis and is useful inthe classification of pituitary adenomas.Adenomatous Polyposis Coli (APC) Rabbit PolyclonalCatalog No. Size FormatE4080 0.1ml ConcentrateE4082 0.5ml ConcentrateE4084 1.0ml ConcentrateE4081 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human APC protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Colon CarcinomaCellular Localization: CytoplasmAKT-1/AKT-2 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10840 0.1ml Concentrate Immunogen: Synthetic peptide from N-terminusE10842 0.5ml Concentrate of human AKT-1.E10844 1.0ml Concentrate Application: IHC-PE10841 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung CarcinomaCellular Localization: CytoplasmHuman Colon Carcinoma stained withanti-Adenomatous Polyposis Coli (APC) antibody E408Human Lung Carcinoma stained withanti-AKT-1/AKT-2 antibody E1084Product Description: APC is associated with structural components of intracellularjunctions, including conductin, and it competes with E-cadherin for binding to specificinternal regions of both β- and γ-catenin. Like APC, APC2 contains SAMP domains, whichare required for conductin binding. Both APC and APC2 regulate the formation of activeβ-catenin-Tcf complexes.Product Description: Two serine/threonine kinases, designated AKT-1 and AKT-2(alternatively referred to as PKB or RacPK), exhibit sequence homology with the proteinkinase A and C families and are encoded by the c-AKT proto-oncogene. AKT-1 and AKT-2contain a Pleckstrin homology (PH) domain and are activated by PDGF stimulation. Thisactivation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the85kDa subunit of the phosphatidylinositol 3-kinase (PI-3 kinase) complex.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 13

AKT-1/PKB (Phospho S473) Rabbit PolyclonalCatalog No. Size FormatE13450 0.1ml ConcentrateE13452 0.5ml ConcentrateE13454 1.0ml ConcentrateE13451 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from theregion of human AKT-1 that contains phosphorylatedsite of serine 473.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusHuman Breast Carcinoma stained withanti-AKT-1/PKB (Phospho S473) antibody E1345alpha Fetoprotein (AFP) Rabbit PolyclonalCatalog No. Size FormatE2950 0.1ml ConcentrateE2952 0.5ml ConcentrateE2954 1.0ml ConcentrateE2951 7.0ml Ready-to-useClone: N/AImmunogen: Human alpha-fetoproteinpurified from human cord serum.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Human alpha-fetoproteinpurified from human cord serum.Cellular Localization: CytoplasmControl Lysate: HepG2Molecular Weight: 70 kDaHuman Fetal Liver stained withanti-alpha Fetoprotein (AFP) antibody E295Product Description: The AKT-1 serine threonine protein kinase is catalytically inactivein serum starved primary and immortalized fibroblasts and activated by plateletderived growth factor. The activation is rapid and specific. Survival factors can suppressapoptosis in a transcription independent manner by activating AKT-1, which thenphosphorylates and inactivates components of the apoptotic machinery. This antibodyis specific to the phosphorylated form of AKT-1.Product Description: AFP is normally synthesized in the liver, intestinal tract, and yolksac of the fetus. Antibody to AFP has been identified in hepatocellular carcinomas (HCC)and germ cell neoplasms, especially yolk sac tumors.ALK/p80 Rabbit MonoclonalCatalog No. Size FormatM3080 0.1ml ConcentrateM3082 0.5ml ConcentrateM3084 1.0ml ConcentrateM3081 7.0ml Ready-to-useClone: SP8Immunogen: Recombinant protein corresponding to aregion which spans the tyrosine kinase catalytic domainand part of the C-terminus of the NPM-ALK transcript.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Anaplastic LymphomaCellular Localization: Cytoplasm, Nucleusalpha-1-Antichymotrypsin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3450 0.1ml Concentrate Immunogen: Purified human alpha-1-antichymotrypsin.E3452 0.5ml Concentrate Application: IHC-PE3454 1.0ml ConcentrateSpecies Reactivity: Human*E3451 7.0ml Ready-to-useIsotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmHuman Anaplastic Lymphoma stained withanti-ALK/p80 antibody M308Human Tonsil stained withanti-alpha-1-Antichymotrypsin antibody E345Product Description: This antibody recognizes a human p80 protein, identified as ahybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM)gene resulting from the t(2;5)(p23;q35) translocation found in a third of large celllymphomas. This antibody can be used to detect p80 in these lymphomas and may alsobe used to detect a subtype of large B cell lymphoma, which expresses the full-lengthALK protein.Product Description: Alpha-1-Antichymotrypsin (Alpha 1-ACT) is an early-stageacute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin,cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associatedwith amyloid plaques in Alzheimer’s disease (AD) and in normal aged human andmonkey brain. Regulation of the serine proteases and serine protease inhibitors plays animportant role in neuromuscular differentiation.ALK/p80 Rabbit PolyclonalCatalog No. Size FormatE1090 0.1ml ConcentrateE1092 0.5ml ConcentrateE1094 1.0ml ConcentrateE1091 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant protein corresponding to aregion which spans the tyrosine kinase catalytic domainand part of the C- terminus of the NPM-ALK transcript.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Anaplastic LymphomaCellular Localization: Cytoplasm, Nucleusalpha-1-Antitrypsin Rabbit PolyclonalCatalog No. Size Format Clone: 5C5.F8.C7E3440 0.1ml Concentrate Immunogen: Purified human alpha-1-antitrypsin.E3442 0.5ml Concentrate Application: IHC-PE3444 1.0ml ConcentrateSpecies Reactivity: Human*E3441 7.0ml Ready-to-useIsotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmHuman Anaplastic Lymphoma stained withanti-ALK/p80 antibody E109Human Tonsil stained withanti-alpha-1-Antitrypsin antibody E344Product Description: This antibody recognizes a human p80 protein, identified as ahybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM)gene resulting from the t(2;5)(p23;q35) translocation found in a third of large celllymphomas. This antibody can be used to detect p80 in these lymphomas and may alsobe used to detect a subtype of large B cell lymphoma, which expresses the full-lengthALK protein.Product Description: Alpha-1-antitrypsin (alpha-1-AT) exists in a number of geneticvariants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and itacts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase,leucocytic proteases, plasmin, and thrombin. In the absence of alpha-1-AT, theseenzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-ATdeficiency is associated with chronic obstructive lung disease (emphysema).14Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Annexin VI Rabbit PolyclonalCatalog No. Size FormatE12380 0.1ml ConcentrateE12382 0.5ml ConcentrateE12384 1.0ml ConcentrateE12381 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide correspondingto N-terminus of human Annexin VI.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver, TonsilCellular Localization: MembraneControl Lysate: 293TMolecular Weight: 75 kDaBAX Rabbit MonoclonalCatalog No. Size FormatM3470 0.1ml ConcentrateM3472 0.5ml ConcentrateM3474 1.0ml ConcentrateM3471 7.0ml Ready-to-useClone: SP47Immunogen: Synthetic peptide corresponding tointernal region of human BAX protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, ColonCellular Localization: Cytoplasm, MembraneHuman Liver stained withanti-Annexin VI antibody E1238Human Breast stained withanti-BAX antibody M347Product Description: Annexin family members have been implicated as regulators ofsuch diverse processes as ion flux, endocytosis and exocytosis and cellular adhesion.Annexin VI is positively regulated by calcium. It contains 8 annexin repeats and it bindsactin filament and lipids. Annexin VI has been localized to stress fibers, membraneruffles, and microspikes.Product Description: BAX is a member of the BCL-2 family of proteins, which function asregulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX canform homodimers and is also able to heterodimerize with other BCL-2 related proteins.Apoptosis Protease Activating Factor 1 (APAF-1) Rabbit PolyclonalCatalog No. Size FormatE3950 0.1ml ConcentrateE3952 0.5ml ConcentrateE3954 1.0ml ConcentrateE3951 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human APAF-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TestisCellular Localization: CytoplasmBAX Rabbit PolyclonalCatalog No. Size FormatE17990 0.1ml ConcentrateE17992 0.5ml ConcentrateE17994 1.0ml ConcentrateE17991 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from internalregion of human BAX protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, ColonCellular Localization: Cytoplasm, MembraneHuman Testis stained withanti-Apoptosis Protease Activating Factor 1 (APAF-1) antibody E395Human Breast stained withanti-BAX antibody E1799Product Description: APAF-1 is a cytosolic protein that rests in a latent state until boundby cytochrome c (APAF-2). The resulting APAF-1/cytochrome c complex associates withthe zymogen form of caspase-9 (APAF-3) in the presence of dATP or ATP, promoting theautocatalytic activation of caspase-9. This antibody can be useful in studies of apoptoticcell death.Product Description: BAX is a member of the BCL-2 family of proteins, which function asregulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX canform homodimers and is also able to heterodimerize with other BCL-2 related proteins.BAK Rabbit PolyclonalCatalog No. Size FormatE3320 0.1ml ConcentrateE3322 0.5ml ConcentrateE3324 1.0ml ConcentrateE3321 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human BAK.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmBCL-2 alpha** Rabbit MonoclonalCatalog No. Size FormatM3660 0.1ml ConcentrateM3662 0.5ml ConcentrateM3664 1.0ml ConcentrateM3661 7.0ml Ready-to-useClone: SP66Immunogen: Synthetic peptide corresponding toN-terminus of human BCL-2 alpha protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-BAK antibody E332Human Tonsil stained withanti-BCL-2 alpha antibody M366Product Description: BAK or BCL-2-homologous antagonist is a member of the BCL-2family of homologous proteins which function in the regulation of apoptosis. TheBCL-2 related proteins interact with one another through the formation of homo- andheterodimers. BAK has been localized by immunohistochemistry to the cytosol of cellsand stains in a punctate pattern characteristic of other BCL-2 family proteins, which havebeen localized to intracellular organelles.Product Description: Expression of BCL-2 alpha oncoprotein inhibits the programmedcell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers expresshigh levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centersare negative.16Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesBCL-2 alpha Rabbit PolyclonalCatalog No. Size FormatE17980 0.1ml ConcentrateE17982 0.5ml ConcentrateE17984 1.0ml ConcentrateE17981 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human BCL-2 alpha protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, MembraneBCL-10 Rabbit PolyclonalCatalog No. Size FormatE19030 0.1ml ConcentrateE19032 0.5ml ConcentrateE19034 1.0ml ConcentrateE19031 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human BCL-10.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmHuman Tonsil stained withanti-BCL-2 alpha antibody E1798Human Tonsil stained withanti-BCL-10 antibody E1903Product Description: Expression of BCL-2 alpha oncoprotein inhibits the programmedcell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers expresshigh levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centersare negative.Product Description: BCL-10/CIPER/CLAP/mE10 is expressed in many human andmurine tissues. It contains a CARD (caspase recruiting domain) domain and is involvedin activation of caspase 9 to induce apoptosis. It is a cellular homolog of equineherpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF-kappaB.Mutations in BCL-10 are observed in many B and T cell lymphomas implicating itsrole in pathogenesis of human cancer.BCL-6 Rabbit PolyclonalCatalog No. Size FormatE14090 0.1ml ConcentrateE14092 0.5ml ConcentrateE14094 1.0ml ConcentrateE14091 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human BCL-6 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusBCL-10 Mouse MonoclonalCatalog No. Size FormatE16760 0.1ml ConcentrateE16762 0.5ml ConcentrateE16764 1.0ml ConcentrateE16761 7.0ml Ready-to-useClone: SPM520Immunogen: Recombinant BCL-10 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: TonsilCellular Localization: Cytoplasm, NucleusHuman Tonsil stained withanti-BCL-6 antibody E1409Human Tonsil stained withanti-BCL-10 antibody E1676Product Description: BCL-6 proto-oncogene product is mainly expressed in normalgerminal center B cells and related lymphomas. BCL-6 is involved in chromosomerearrangements at 3q27 in non-Hodgkin’s lymphomas and BCL-6 rearrangementshave been detected in diffuse large B cell lymphomas. BCL-6 has been detectedimmunohistochemically in follicular lymphomas, diffuse large B cell lymphomas,Burkitt lymphomas and in nodular, lymphocyte predominant Hodgkin’s disease.Product Description: BCL-10/CIPER/CLAP/mE10 is expressed in many human andmurine tissues. It contains a CARD (caspase recruiting domain) domain and is involvedin activation of caspase 9 to induce apoptosis. It is a cellular homolog of equineherpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF-kappaB.Mutations in BCL-10 are observed in many B and T cell lymphomas implicating itsrole in pathogenesis of human cancer.BCL-10 Rabbit PolyclonalCatalog No. Size FormatE19240 0.1ml ConcentrateE19242 0.5ml ConcentrateE19244 1.0ml ConcentrateE19241 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide near the N-terminus ofhuman BCL-10 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, NucleusBCL-X Rabbit PolyclonalCatalog No. Size FormatE3370 0.1ml ConcentrateE3372 0.5ml ConcentrateE3374 1.0ml ConcentrateE3371 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic protein derived fromnear N-terminus of the human BCL-X protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s LymphomaCellular Localization: Cytoplasm,Membrane, NucleusControl Lysate: 293TMolecular Weight: 27 kDaHuman Tonsil stained withanti-BCL-10 antibody E1924Human Hodgkin’s Lymphoma stained withanti-BCL-X antibody E337Product Description: BCL-10 is a cytoplasmic protein expressed at different levelsin most normal human cells and translocates into the nucleus of MALT lymphoma,especially in those with translocation (1;14)(p22;q32). BCL-10 and MALT-1 protein forma complex in MALT lymphoma. They are thought to synergize in the activation ofNF-kappaB, and may contribute to the same pathogenic process that leads tomalignancy. Mutations in BCL-10 are observed in some B and T cell lymphomas.Product Description: BCL-X is a member of the BCL-2 family of proteins, which functionas regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acidprotein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain thatis well conserved among members of the BCL-2 family.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 17

BCL-XL Rabbit PolyclonalCatalog No. Size FormatE4200 0.1ml ConcentrateE4202 0.5ml ConcentrateE4204 1.0ml ConcentrateE4201 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human BCL-X protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon Carcinoma,Hodgkin’s LymphomaCellular Localization: Cytoplasm,Membrane, NucleusControl Lysate: 293TMolecular Weight: 27 kDaHuman Colon Carcinoma stained withanti-BCL-XL antibody E420Product Description: BCL-X is a member of the BCL-2 family of proteins, which functionas regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acidprotein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domainthat is well conserved among members of the BCL-2 family.Bnip3L Rabbit MonoclonalCatalog No. Size FormatE16840 0.1ml ConcentrateE16842 0.5ml ConcentrateE16844 1.0ml ConcentrateE16841 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human BNIP3L protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Bnip3L antibody E1684Product Description: BCL2/adenovirus E1B 19kDa protein-interacting protein 3-like(Bnip3L) is a protein encoded by the BNIP3L gene in humans. Overexpression of Bnip3Linduces apoptosis. Bnip3L interacts with and overcomes suppression by BCL-2 andBCL-XL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L isubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily ofthe pro-apoptotic mitochondrial proteins.BCL-XL Mouse MonoclonalCatalog No. Size FormatE16660 0.1ml ConcentrateE16662 0.5ml ConcentrateE16664 1.0ml ConcentrateE16661 7.0ml Ready-to-useClone: 7D9Immunogen: Synthetic peptide corresponding toN-terminus of mouse BCL-XL protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG2aControl Tissue: Hodgkin’s LymphomaCellular Localization: Cytoplasm, Membrane, NucleusBOB-1 Rabbit MonoclonalCatalog No. Size FormatM3920 0.1ml ConcentrateM3922 0.5ml ConcentrateM3924 1.0ml ConcentrateM3921 7.0ml Ready-to-useClone: SP92Immunogen: Synthetic peptide correspondingto C-terminus of human BOB-1 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusControl Lysate: RamosMolecular Weight: 35 kDaHuman Hodgkin’s Lymphoma stained withanti-BCL-XL antibody E1666Human Tonsil stained withanti-BOB-1 antibody M392Product Description: BCL-X is a member of the BCL-2 family of proteins, which functionas regulators of apoptosis. BCL-X has two isoforms, BCL-XL (long) a 241 amino acidprotein and BCL-XS (short) a 178 amino acid protein lacking a 63 amino acid domain thatis well conserved among members of the BCL-2 family.Product Description: B cell Oct binding protein 1 (BOB-1), is a transcription factor thatis required for germinal center formation and immunoglobulin production. BOB-1 isexpressed at high levels in spleen and peripheral blood leukocytes and represents anOCT co-factor capable of conferring cell specific activation of OCT-1 and OCT-2.BIM/BOD Rabbit PolyclonalCatalog No. Size FormatE4360 0.1ml ConcentrateE4362 0.5ml ConcentrateE4364 1.0ml ConcentrateE4361 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human BIM.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmBOB-1 Rabbit PolyclonalCatalog No. Size FormatE16670 0.1ml ConcentrateE16672 0.5ml ConcentrateE16674 1.0ml ConcentrateE16671 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminusof human BOB-1.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusControl Lysate: RamosMolecular Weight: 35 kDaHuman Placenta stained withanti-BIM/BOD antibody E436Human Tonsil stained withanti-BOB-1 antibody E1667Product Description: BIM/BOD (BCL-2-related Ovarian Death Gene) belongs to BCL-2family of proteins containing BCL-2 homology domain3 (BH3). It is proapoptotic andexerts its effects by interacting with prosurvival members of the BCL-2 family like BCL-2,BCL-XL and BCL-W. It exhibits three splice variants; BIML, BIMS and BIMEL. They all sharehomology in their C-terminus BH3 domain and are ubiquitously expressed. BIMS is thestrongest of them with respect to the proapoptotic capacity.Product Description: B cell Oct binding protein 1 (BOB-1), is a transcription factor thatis required for germinal center formation and immunoglobulin production. BOB-1 isexpressed at high levels in spleen and peripheral blood leukocytes and represents anOCT co-factor capable of conferring cell specific activation of OCT-1 and OCT-2.18Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesBone Morphogenetic Protein 4 (BMP-4) Rabbit PolyclonalCatalog No. Size Format Clone: N/AE12510 0.1ml Concentrate Immunogen: Recombinant human BMP-4 protein.E12510 0.5ml Concentrate Application: IHC-PE12514 1.0ml ConcentrateSpecies Reactivity: Human*E12511 7.0ml Ready-to-useIsotype: Rabbit IgGControl Tissue: Bone MarrowCellular Localization: Cytoplasmc-Abl Rabbit PolyclonalCatalog No. Size FormatE4000 0.1ml ConcentrateE4002 0.5ml ConcentrateE4004 1.0ml ConcentrateE4001 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from the sequenceadjacent to protein tyrosine kinase domain ofhuman c-Abl.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Bone Marrow stained withanti-Bone Morphogenetic Protein 4 (BMP-4) antibody E1251Human Placenta stained withanti-c-Abl antibody E400Product Description: Bone morphogenetic protein 4 (BMP4) is one of nine structurallyrelated BMPs belonging to the transforming growth factor beta superfamily of secretedproteins. BMP4 has important roles as a signaling molecule in the developing centraland peripheral nervous system, musculature and skeleton. BMP also plays an importantrole in early tooth morphogenesis.Product Description: The c-Abl proto-oncogene encodes a protein tyrosine kinasethat is located in the cytoplasm and nucleus. In chronic myelogenous leukemia andin a subset of acute lymphoblastic leukemias, the c-Abl proto-oncogene undergoesa (9;22) chromosomal translocation producing a novel rearranged chromosome (thePhiladelphia chromosome). The molecular consequence of this translocation is thegeneration of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.C4d** Rabbit MonoclonalCatalog No. Size FormatM3910 0.1ml ConcentrateM3912 0.5ml ConcentrateM3914 1.0ml ConcentrateM3911 7.0ml Ready-to-useClone: SP91Immunogen: Synthetic peptide corresponding toC-terminus of human C4d protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Secreted ProteinCadherin-E/E-Cadherin** Rabbit MonoclonalCatalog No. Size FormatM3640 0.1ml ConcentrateM3642 0.5ml ConcentrateM3644 1.0ml ConcentrateM3641 7.0ml Ready-to-useClone: SP64Immunogen: Recombinant proteinencoding aa 600-707 of human cadherin-E.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, ColonCellular Localization: MembraneControl Lysate: MCF7Molecular Weight: 135 kDaHuman Tonsil stained withanti-C4d antibody M391Human Breast Carcinoma stained withanti-Cadherin-E/E-Cadherin antibody M364Product Description: C4 plays a central role in the activation of the classical pathway ofthe complement system. Prior to secretion, the single-chain precursor is enzymaticallycleaved to yield the non-identical chains (alpha, beta and gamma). During activation,the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta andgamma chains. Further degradation of C4b by C1 into the inactive fragments C4c andC4d blocks the generation of C3 convertase.Product Description: Cadherin-E/E-cadherin (uvomorulin, cell-CAM120/80) is a calciumdependent cell adhesion molecule expressed predominately in epithelial tissues. It playsan important role in the growth and development of cells via the mechanisms of controlof tissue architecture and the maintenance of tissue integrity.C4d Rabbit PolyclonalCatalog No. Size FormatE17340 0.1ml ConcentrateE17342 0.5ml ConcentrateE17344 1.0ml ConcentrateE17341 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide of human C4d.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, TonsilCellular Localization: Secreted ProteinCadherin-E/E-Cadherin Mouse MonoclonalCatalog No. Size FormatE8640 0.1ml ConcentrateE8642 0.5ml ConcentrateE8644 1.0ml ConcentrateE8641 7.0ml Ready-to-useClone: SPM471Immunogen: Recombinant protein encoding aa600-707 of human cadherin-E.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG2b/kControl Tissue: Breast, Skin, TonsilCellular Localization: MembraneHuman Tonsil stained withanti-C4d antibody E1734Human Breast stained withanti-Cadherin-E/E-Cadherin antibody E864Product Description: C4 plays a central role in the activation of the classical pathway ofthe complement system. Prior to secretion, the single-chain precursor is enzymaticallycleaved to yield the non-identical chains (alpha, beta and gamma). During activation,the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta andgamma chains. Further degradation of C4b by C1 into the inactive fragments C4c andC4d blocks the generation of C3 convertase.Product Description: E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependentcell adhesion molecule expressed predominately in epithelial tissues. It plays animportant role in the growth and development of cells via the mechanisms of controlof tissue architecture and the maintenance of tissue integrity.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 19

Primary AntibodiesCalcitonin Rabbit PolyclonalCalpain Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE1150 0.1ml ConcentrateE1152 0.5ml ConcentrateImmunogen: Synthetic human calcitonin1-32 amino acid peptide.E18780 0.1ml ConcentrateE18782 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human calpain protein.E1154 1.0ml ConcentrateApplication: IHC-P, WBE18784 1.0ml ConcentrateApplication: IHC-PE1151 7.0ml Ready-to-useSpecies Reactivity: Human*E18781 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Thyroid, Thyroid MedullaryCarcinomaCellular Localization: CytoplasmControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: Human ThyroidMolecular Weight: 14 kDaHuman Thyroid stained withanti-Calcitonin antibody E115Human Breast Carcinoma stained withanti-Calpain antibody E1878Product Description: Calcitonin is a 32 amino acid peptide which can be demonstratedin C cells of the normal and hyperplastic thyroid. Calcitonin is expressed in a spectrumof C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors.Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granularpattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varyingdegrees of calcitonin activity.Product Description: Calpains are a ubiquitous, well-conserved family ofcalcium-dependent, cysteine proteases. Calpain families have been implicated inneurodegenerative processes due to calcium influx and oxidative stress. Calpain I and IIare heterodimeric with distinct large subunits associated with common small subunits,all of which are encoded by different genes. Calpain S1 is a small subunit common toboth Calpain I and II and is associated with myotonic dystrophy.Calcium Sensing Receptor (CaSR) Rabbit PolyclonalCatalog No. Size FormatE10620 0.1ml ConcentrateE10622 0.5ml ConcentrateE10624 1.0ml ConcentrateE10621 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from N-terminus of ratcalcium sensing receptor.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmCalpastatin Rabbit MonoclonalCatalog No. Size Format Clone: SP82M3820 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearM3822 0.5ml Concentrate C-terminus of human calpastatin protein.M3824 1.0ml Concentrate Application: IHC-PM3821 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Placenta stained withanti-Calcium Sensing Receptor (CaSR) antibody E1062Human Breast Carcinoma stained withanti-Calpastatin antibody M382Product Description: The calcium sensing receptor (CaSR) has been shown toplay a major role in regulating parathyroid hormone secretion and subsequentlyinfluencing the calcium concentration of extracellular fluids. Several disorders ofcalcium homeostasis have been linked to mutations in the CaSR. These include familialhypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), andautosomal dominant hypocalemia (ADHypo).Product Description: Calpastatin is an endogenous calpain (calcium-dependentcysteine protease) inhibitor. It is involved in the proteolysis of amyloid precursor protein.The calpain/calpastatin system is involved in numerous membrane fusion events, suchas neural vesicle exocytosis and platelet and red-cell aggregation. Calpastatin, a specificendogenous inhibitor of calpain, functions by binding to calpain. It contains mutlipleinhibitory fragments for calpain activity.Calpain Rabbit MonoclonalCatalog No. Size FormatM3810 0.1ml ConcentrateM3812 0.5ml ConcentrateM3814 1.0ml ConcentrateM3811 7.0ml Ready-to-useClone: SP81Immunogen: Synthetic peptide derived frominternal region of human calpain protein.Application: IHC-P, WBSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: 293TMolecular Weight: 28 kDaCalpastatin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18790 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearE18792 0.5ml Concentrate C-terminus of human calpastatin protein.E18794 1.0ml Concentrate Application: IHC-PE18791 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Calpain antibody M381Human Breast Carcinoma stained withanti-Calpastatin antibody E1879Product Description: Calpains are a ubiquitous, well-conserved family ofcalcium-dependent, cysteine proteases. Calpain families have been implicated inneurodegenerative processes due to calcium influx and oxidative stress. Calpain I and IIare heterodimeric with distinct large subunits associated with common small subunits,all of which are encoded by different genes. Calpain S1 is a small subunit common toboth Calpain I and II and is associated with myotonic dystrophy.Product Description: Calpastatin is an endogenous calpain (calcium-dependentcysteine protease) inhibitor. It is involved in the proteolysis of amyloid precursor protein.The calpain/calpastatin system is involved in numerous membrane fusion events, suchas neural vesicle exocytosis and platelet and red-cell aggregation. Calpastatin, a specificendogenous inhibitor of calpain, functions by binding to calpain. It contains mutlipleinhibitory fragments for calpain activity.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 21

Calretinin Rabbit MonoclonalCatalog No. Size Format Clone: SP13M3130 0.1ml Concentrate Immunogen: Recombinant full length mouseM3132 0.5ml Concentrate calretinin protein.M3134 1.0ml Concentrate Application: IHC-PM3131 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, MesotheliomaCellular Localization: Cytoplasm, NucleusCaM Kinase Kinase alpha Rabbit PolyclonalCatalog No. Size FormatE13850 0.1ml ConcentrateE13852 0.5ml ConcentrateE13854 1.0ml ConcentrateE13851 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human CaM kinase kinase alpha.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: NucleusHuman Mesothelioma stained withanti-Calretinin antibody M313Human Brain stained withanti-CaM Kinase Kinase alpha antibody E1385Product Description: Calretinin is an intracellular calcium-binding protein belongingto the troponin C superfamily. The immunohistochemical detection of calretinin indeveloping cerebellum is restricted to the later stages indicated by weak staining fromweek 21 of gestation in Purkinje and basket cells and in neurons of the dentate nucleus.In tumors, calretinin has been detected in mesotheliomas and some pulmonaryadenocarcinomas.Product Description: Ca2+/Calmodulin dependent protein kinases (CaM Kinase I, II andIV) are considered to play a central role in many cellular functions in response to Ca2+signaling, including synthesis and secretion of neurotransmitters, axonal transport,long term potentiation (LTP), receptor function, modification of the cytoskeleton andregulation of gene expression.Calretinin Rabbit PolyclonalCatalog No. Size FormatE1170 0.1ml ConcentrateE1172 0.5ml ConcentrateE1174 1.0ml ConcentrateE1171 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant full length mousecalretinin protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, MesotheliomaCellular Localization: Cytoplasm, NucleusControl Lysate: Human BrainMolecular Weight: 29 kDaCarbonic Anhydrase 9 (CA-9) Rabbit MonoclonalCatalog No. Size FormatM4060 0.1ml ConcentrateM4062 0.5ml ConcentrateM4064 1.0ml ConcentrateM4061 7.0ml Ready-to-useClone: SP106Immunogen: Synthetic peptide derived fromhuman CA-9.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: Membrane, NucleusHuman Mesothelioma stained withanti-Calretinin antibody E117Human Stomach stained withanti-Carbonic Anhydrase 9 (CA-9) antibody M406Product Description: Calretinin is an intracellular calcium-binding protein belongingto the troponin C superfamily. The immunohistochemical detection of calretinin indeveloping cerebellum is restricted to the later stages indicated by weak staining fromweek 21 of gestation in Purkinje and basket cells and in neurons of the dentate nucleus.In tumors, calretinin has been detected in mesotheliomas and some pulmonaryadenocarcinomas.Product Description: Carbonic anhydrase (CA) is an enzyme that assists rapid interconversionof carbon dioxide and water into carbonic acid, protons, and bicarbonateions. It is abundant in all mammalian tissues. CA-9 is one of the most inducible genesbecause of its stability and location within the membrane. Carbonic anhydrases have awidespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux.CA-9 is a reliable histochemical marker of hypoxia.CaM Kinase IV Rabbit PolyclonalCatalog No. Size FormatE10600 0.1ml ConcentrateE10602 0.5ml ConcentrateE10604 1.0ml ConcentrateE10601 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminus of ratCaM kinase IV.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: Cytoplasm, NucleusCarbonic Anhydrase 9 (CA-9) Rabbit PolyclonalCatalog No. Size FormatE18420 0.1ml ConcentrateE18422 0.5ml ConcentrateE18424 1.0ml ConcentrateE18421 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derivedfrom human CA-9.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: Membrane, NucleusControl Lysate: RamosMolecular Weight: 50 kDaHuman Brain stained withanti-CaM Kinase IV antibody E1060Human Stomach stained withanti-Carbonic Anhydrase 9 (CA-9) antibody E1842Product Description: Calmodulin dependent protein kinases (CaM kinases) consist ofa family of Ser/Thr protein kinases including CaMKI, CaMKII and CaMKIV. CaMKs play acentral role in many cellular functions in response to Ca2+ signaling, including synthesisand secretion of neurotransmitters, axonal transport, long term potentiation etc. CaMkinase IV is abundantly expressed in the brain and in the thymus and is localized to thecytoplasm and nucleus.Product Description: Carbonic anhydrase (CA) is an enzyme that assists rapidinterconversion of carbon dioxide and water into carbonic acid, protons, andbicarbonate ions. It is abundant in all mammalian tissues. Carbonic anhydrases have awidespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) fluxand it can be used as a histochemical marker of hypoxia.22Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCAS Rabbit PolyclonalCatalog No. Size FormatE10810 0.1ml ConcentrateE10812 0.5ml ConcentrateE10814 1.0ml ConcentrateE10811 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman CAS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver CarcinomaCellular Localization: CytoplasmCaspase-10 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10850 0.1ml Concentrate Immunogen: Synthetic peptide from C-terminus ofE10852 0.5ml Concentrate human caspase-10.E10854 1.0ml Concentrate Application: IHC-PE10851 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver CarcinomaCellular Localization: CytoplasmHuman Liver Carcinoma stained withanti-CAS antibody E1081Human Liver Carcinoma stained withanti-Caspase-10 antibody E1085Product Description: CAS (Cellular Apoptosis Susceptibility) contains 971 amino acidswith 59% overall sequence homology as compared to yeast chromosome segregationhomolog, CSE1. CAS is highly expressed in testis and fetal liver. CAS expression inhuman fibroblasts increases as cells are induced to proliferate and decreases in growtharrested cells.Product Description: Caspase-10 is a member of the Ced-3 cystein protease family andit has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.Caspase-1 to-10 function as key components of the apoptotic machinery and act todestroy specific target proteins which are critical to cellular longevity.Caspase-1 Rabbit PolyclonalCatalog No. Size FormatE3910 0.1ml ConcentrateE3912 0.5ml ConcentrateE3914 1.0ml ConcentrateE3911 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from internalregion of human caspase-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-Caspase-1 antibody E391Catenin-beta Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2260 0.1ml Concentrate Immunogen: Synthetic peptide fromE2262 0.5ml Concentrate C-terminus of the catenin-beta protein.E2264 1.0ml Concentrate Application: IHC-P, WBE2261 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm,MembraneControl Lysate: A431Molecular Weight: 92 kDaHuman Breast Carcinoma stained withanti-Catenin-beta antibody E226Product Description: Caspase-1/ICE (Interleukin-1 Beta Converting Enzyme) isexpressed as a proenzyme of 45kDa in many tissues and is activated by proteolyticcleavage. Active caspase-1 is a tetramer of two subunits p10 and p20. Caspase-1 (cysteinaspartic-specific protease 1) cleaves interleukin beta into its active form which furtherexerts its proinflammatory effects. Overexpression of caspase-1 can induce apoptosisin fibroblasts.Product Description: The catenins (α, β and γ) are ubiquitously expressed, cytoplasmicproteins associated with E-cadherin at cellular junctions. β-catenin also binds toN-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes arelinked to the cytoskeleton via a direct association between α-actin and α-catenin.Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencingcellular adhesion.Caspase-8 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2280 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toE2282 0.5ml Concentrate N-terminus of human caspase-8 protein.E2284 1.0ml Concentrate Application: IHC-PE2281 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: CytoplasmCatenin-gamma Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3680 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE3682 0.5ml Concentrate C-terminus of human catenin-gamma protein.E3684 1.0ml Concentrate Application: IHC-PE3681 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Stomach stained withanti-Caspase-8 antibody E228Human Breast Carcinoma stained withanti-Catenin-gamma antibody E368Product Description: Caspase-8 binds to the death effector domain (DED) throughan analogous DED domain. Recruitment of caspase-8 to the Fas receptor resultsin oligomerization of the caspase-8 protein, which in turn drives its autoactivationthrough “self-cleavage.” Activated caspase-8 then activates other downstream caspasesincluding caspase-9, thereby commiting the cell to undergo apoptosis. This antibodyalso detects the processed forms (42/44kDa, 25kDA, and 14kDa) of caspase-8.Product Description: The catenins (α, β and γ) are ubiquitously expressed, cytoplasmicproteins that associate with E-cadherin. α-catenin can also bind P-cadherin andN-cadherin. γ-catenin, also known as plakoglobin, is an 82kDa protein that bindswith α-catenin and N-cadherin.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 23

Cathepsin D Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2820 0.1ml Concentrate Immunogen: Recombinant human cathepsin D protein.E2822 0.5ml Concentrate Application: IHC-PE2824 1.0ml ConcentrateSpecies Reactivity: Human*E2821 7.0ml Ready-to-useIsotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmCaveolin-1 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10780 0.1ml Concentrate Immunogen: Synthetic peptide from N-terminus ofE10782 0.5ml Concentrate human caveolin-1.E10784 1.0ml Concentrate Application: IHC-PE10781 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung CarcinomaCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-Cathepsin D antibody E282Human Lung Carcinoma stained withanti-Caveolin-1 antibody E1078Product Description: Cathepsin D is synthesized as a 54kDa precursor and isproteolytically processed to an intermediate 48kDa single chain. The 48KDa form isfurther processed into the more stable 34kDa and 14kDa mature forms. Cathepsin Dis an estrogen-regulated lysosomal protease that facilitates cancer cell migration andinvasion by digesting the basement membrane, extracellular matrix, and connectivetissue. Cathepsin D is expressed in epithelial cells as well as in macrophages.Product Description: Caveolae are 50-100 nm flask-shaped subcompartmentof the plasma membrane. On the basis of morphological studies, caveolae havebeen implicated to function in the transcytosis of various macromolecules acrosscapillary endothelial cells, uptake of small molecules via potocytosis and thecompartmentalization of certain signaling molecules or receptors. Three proteins,caveolin-1, caveolin-2 and caveolin-3 have been identified as principle componentsof caveolae.Caveolin-1 Rabbit MonoclonalCatalog No. Size Format Clone: SP43M3430 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toM3432 0.5ml Concentrate C-terminus of human caveolin-1 protein.M3434 1.0ml Concentrate Application: IHC-PM3431 7.0ml Ready-to-use Species Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: Lung, Lung CarcinomaCellular Localization: Cytoplasm, MembraneCBP/p300-Interacting Transactivator 1 (CITED-1) Rabbit PolyclonalCatalog No. Size FormatE3510 0.1ml ConcentrateE3512 0.5ml ConcentrateE3514 1.0ml ConcentrateE3511 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant full-length humanCITED-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PapillaryThyroid CarcinomaCellular Localization: Cytoplasm, NucleusHuman Lung Carcinoma stained withanti-Caveolin-1 antibody M343Human Breast Carcinoma stained withanti-CBP/p300-Interacting Transactivator 1 (CITED-1) antibody E351Product Description: Caveolae are 50-100 nm flask-shaped subcompartmentof the plasma membrane. On the basis of morphological studies, caveolae havebeen implicated to function in the transcytosis of various macromolecules acrosscapillary endothelial cells, uptake of small molecules via potocytosis and thecompartmentalization of certain signaling molecules or receptors. Three proteins,caveolin-1, caveolin-2 and caveolin-3 have been identified as principle componentsof caveolae.Product Description: CITED1 (CBP/p300-Interacting Transactivator 1)/Melanocytespecificprotein 1 is highly expressed in most of the papillary thyroid carcinoma. It is notexpressed in normal tissues except normal breast epithelium. Studies show that thisprotein functions as a selective coactivator for estrogen-dependent transcription.Caveolin-1 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17640 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE17642 0.5ml Concentrate C-terminus of human caveolin-1.E17644 1.0ml Concentrate Application: IHC-PE17641 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung, Lung CarcinomaCellular Localization: Cytoplasm, MembraneCCR7 Rabbit PolyclonalCatalog No. Size FormatE18460 0.1ml ConcentrateE18462 0.5ml ConcentrateE18464 1.0ml ConcentrateE18461 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromhuman CCR7.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Small IntestineCellular Localization: MembraneHuman Lung stained withanti-Caveolin-1 antibody E1764Human Small Intestine stained withanti-CCR7 antibody E1846Product Description: Caveolae are 50-100 nm flask-shaped subcompartmentof the plasma membrane. On the basis of morphological studies, caveolae havebeen implicated to function in the transcytosis of various macromolecules acrosscapillary endothelial cells, uptake of small molecules via potocytosis and thecompartmentalization of certain signaling molecules or receptors. Three proteins,caveolin-1, caveolin-2 and caveolin-3 have been identified as principle componentsof caveolae.Product Description: C-C chemokine receptor type 7 (CCR7) is a member of the Gprotein coupled receptor family (subfamily: chemokine). This receptor was identified asa gene induced by the Epstein Barr virus (EBV). It is expressed particularly in lymphoidtissues and in activated B and T lymphocytes and has been shown to control themigration of memory T cells to inflamed tissues, as well as stimulate dendriticcell maturation.24Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCCR8 Rabbit PolyclonalCatalog No. Size FormatE10790 0.1ml ConcentrateE10792 0.5ml ConcentrateE10794 1.0ml ConcentrateE10791 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from aa 183-193 ofhuman CCR8.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ThymusCellular Localization: MembraneCD3 Rabbit MonoclonalCatalog No. Size FormatM3070 0.1ml ConcentrateM3072 0.5ml ConcentrateM3074 1.0ml ConcentrateM3071 7.0ml Ready-to-useClone: SP7Immunogen: Synthetic peptide correspondingto internal region of the epsilon chain of humanCD3 protein.Application: IHC-P, WBSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneControl Lysate: JurkatMolecular Weight: 20 kDaHuman Thymus stained withanti-CCR8 antibody E1079Human Tonsil stained withanti-CD3 antibody M307Product Description: C-C chemokine receptor type 8 (CCR8) encodes a member ofthe beta chemokine receptor family. This receptor protein preferentially expressesin the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophageinflammatory protein-1 beta (MIP1 beta) have been identified as ligands of thisreceptor. CCR8 expression has been reported in spleen and thymus, natural-killer cells,monocytes, and T cells, and at lower levels in peripheral blood leukocytes.Product Description: This antibody reacts with the intracytoplasmic portion of the CD3antigen expressed by T cells. It stains human T cells in both the cortex and medulla ofthe thymus and in peripheral lymphoid tissues. This antibody is suitable for stainingnormal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.CD1 alpha Rabbit PolyclonalCatalog No. Size FormatE4710 0.1ml ConcentrateE4712 0.5ml ConcentrateE4714 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Recombinant protein encoding aa19-327 of human CD1 alpha.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: K562Molecular Weight: 49 kDaCD3 Rabbit PolyclonalCatalog No. Size FormatE1250 0.1ml ConcentrateE1252 0.5ml ConcentrateE1254 1.0ml ConcentrateE1251 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic 13-mer peptidecorresponding to internal region of theepsilon chain of human CD3 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneControl Lysate: JurkatMolecular Weight: 19 kDaN/A E471Human Tonsil stained withanti-CD3 antibody E125Product Description: At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 isexpressed on cortical thymocytes, Langerhans cells, and dendritic cells. It is absent onmature peripheral blood T cells but intracytoplasmic expression is detected on activatedT lymphocytes. CD1 proteins have been demonstrated to restrict T-cell response tonon-peptide lipid and glycolipid antigens and play a role in non-classical antigenpresentation.Product Description: This antibody reacts with the intracytoplasmic portion of the CD3antigen expressed by T cells. It stains human T cells in both the cortex and medulla ofthe thymus and in peripheral lymphoid tissues. This antibody is suitable for stainingnormal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.CD2 Rabbit PolyclonalCatalog No. Size FormatE3040 0.1ml ConcentrateE3042 0.5ml ConcentrateE3044 1.0ml ConcentrateE3041 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant protein encoding aa25-209 of human CD2 .Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneControl Lysate: JurkatMolecular Weight: 45-58 kDaCD3 zeta Rabbit PolyclonalCatalog No. Size FormatE3050 0.1ml ConcentrateE3052 0.5ml ConcentrateE3054 1.0ml ConcentrateE3051 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from nearN-terminus of the zeta chain of human CD3 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneHuman Tonsil stained withanti-CD2 antibody E304Human Tonsil stained withanti-CD3 zeta antibody E305Product Description: The CD2 antigen (LFA-2) is a monomeric 45 to 58kDa glycoprotein.It is an accessory molecule important in mediating the adhesion of activated T cells andthymocytes with antigen-presenting cells and target cells. This antibody reacts with themajority of T cells in peripheral lymphoid tissue, NK cells, cortical thymocytes and mostmalignant cells of T cell origin.Product Description: CD3 consists of five invariant polypeptide chains that associate toform three dimers: a heterodimer of gamma and epsilon chains, a heterodimer of deltaand epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and etachains. The zeta chain contains three consecutive copies of the same motif.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 25

Primary AntibodiesCD8 Rabbit MonoclonalCatalog No. Size FormatM3160 0.1ml ConcentrateM3162 0.5ml ConcentrateM3164 1.0ml ConcentrateM3161 7.0ml Ready-to-useClone: SP16Immunogen: Synthetic peptide corresponding toC-terminus of alpha chain of the human CD8 molecule.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD10** Rabbit MonoclonalCatalog No. Size FormatM3670 0.1ml ConcentrateM3672 0.5ml ConcentrateM3674 1.0ml ConcentrateM3671 7.0ml Ready-to-useClone: SP67Immunogen: Synthetic peptidecorresponding to human CD10.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Renal Cell Carcinoma, TonsilCellular Localization: Cytoplasm,MembraneControl Lysate: RamosMolecular Weight: 100 kDaHuman Tonsil stained withanti-CD8 antibody M316Human Renal Cell Carcinoma stained withanti-CD10 antibody M367Product Description: The CD8 molecule consists of two chains, termed α and β chain,which are expressed as a disulfide-linked α/β heterdimer or as an α/α homodimer onT cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as α/βheterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC classI/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).Product Description: CD10, also known as the Common Acute Lymphocytic LeukemiaAntigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity.CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular lymphomas,immature B cells, chronic myelocytic leukemia, and renal clear cell carcinoma. It is alsopresent on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially highexpression on the brush border kidney, and gut epithelial cells.CD8 Rabbit PolyclonalCatalog No. Size FormatE2480 0.1ml ConcentrateE2482 0.5ml ConcentrateE2484 1.0ml ConcentrateE2481 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of the cytoplasmic domain of alpha chain ofthe CD8 molecule.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD10 Rabbit PolyclonalCatalog No. Size FormatE18270 0.1ml ConcentrateE18272 0.5ml ConcentrateE18274 1.0ml ConcentrateE18271 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human CD10.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, TonsilCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-CD8 antibody E248Human Kidney stained withanti-CD10 antibody E1827Product Description: The CD8 molecule consists of two chains, termed α and β chain,which are expressed as a disulfide-linked α/β heterdimer or as an α/α homodimer onT cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as α/βheterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC classI/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).Product Description: CD10, also known as the Common Acute Lymphocytic LeukemiaAntigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity.CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular lymphomas,immature B cells, chronic myelocytic leukemia, and renal clear cell carcinoma. It is alsopresent on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially highexpression on the brush border kidney, and gut epithelial cells.CD9 Rabbit PolyclonalCatalog No. Size FormatE11010 0.1ml ConcentrateE11012 0.5ml ConcentrateE11014 1.0ml ConcentrateE11011 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant human full-lengthCD9 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD19 Rabbit MonoclonalCatalog No. Size FormatM4100 0.1ml ConcentrateM4102 0.5ml ConcentrateM4104 1.0ml ConcentrateM4101 7.0ml Ready-to-useClone: SP110Immunogen: Synthetic peptide corresponding toC-terminus of human CD19.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneHuman Tonsil stained withanti-CD9 antibody E1101Human Tonsil stained withanti-CD19 antibody M410Product Description: The CD9 antigen is a glycoprotein expressed on the surface ofdeveloping B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated Tlymphocytes and by neurons and glial cells in the peripheral nervous system. In pre-Bcells and platelets, CD9 antigen regulates cell activation aggregation possibly throughan association with the integrin CD41/CD61. It also regulates cell motility in a variety ofcell lines.Product Description: CD19 is expressed in B lymphocytes and B cell lymphoma. It mayalso be present on some early myeloid progenitors, particularly those of the monoblastictype. It appears to be expressed on myeloid leukemia cells, particularly those ofmonocytic lineage. Leukemia phenotype studies have demonstrated that the earliestand broadest B cell restricted antigen is the CD19 antigen. CD19 is negative inT lymphocytes, plasma cells, and their tumors.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 27

CD19 Rabbit PolyclonalCatalog No. Size FormatE18960 0.1ml ConcentrateE18962 0.5ml ConcentrateE18964 1.0ml ConcentrateE18961 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human CD19 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD22** Rabbit MonoclonalCatalog No. Size FormatM4040 0.1ml ConcentrateM4042 0.5ml ConcentrateM4044 1.0ml ConcentrateM4041 7.0ml Ready-to-useClone: SP104Immunogen: Synthetic peptide corresponding toC-terminus of human CD22.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-CD19 antibody E1896Human Tonsil stained withanti-CD22 antibody M404Product Description: CD19 is expressed in B lymphocytes and B-cell lymphoma. Itmay also be present on some early myeloid progenitors, particularly those of themonoblastic type. It appears to be expressed on myeloid leukemia cells, particularlythose of monocytic lineage. Leukemia phenotype studies have demonstrated that theearliest and broadest B cell restricted antigen is the CD19 antigen. CD19 is negative in Tlymphocytes, plasma cells, and their tumors.Product Description: CD22 is a type 1 integral membrane glycoprotein expressed inboth the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early inB cell lymphocyte differentiation at approximately the same stage as the CD19 antigen.CD22 membrane expression is limited to the late differentiation stages. There are twodifferent isoforms of CD22. It exists as a monomer and heterodimer. CD22 is stronglyexpressed in hairy cell leukemia.CD20 Rabbit MonoclonalCatalog No. Size FormatM3320 0.1ml ConcentrateM3322 0.5ml ConcentrateM3324 1.0ml ConcentrateM3321 7.0ml Ready-to-useClone: SP32Immunogen: Synthetic peptide fromC-terminus of human CD20 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: B Cell Lymphoma, TonsilCellular Localization: Cytoplasm,MembraneControl Lysate: RamosMolecular Weight: 35 kDaCD22 Rabbit PolyclonalCatalog No. Size FormatE19020 0.1ml ConcentrateE19022 0.5ml ConcentrateE19024 1.0ml ConcentrateE19021 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human CD22.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, MembraneHuman B Cell Lymphoma stained withanti-CD20 antibody M332Human Tonsil stained withanti-CD22 antibody E1902Product Description: CD20 is a leukocyte surface antigen consisting of fourtransmembrane regions and cytoplasmic N- and C-termini. CD20 is expressedprimarily on B cells but has also been detected on both normal and neoplastic T cells.Activation of CD20 significantly increases DNA synthesis and is thought to involve basichelix-loop-helix leucine zipper transcription factors.Product Description: CD22 is a type 1 integral membrane glycoprotein expressed inboth the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early inB cell lymphocyte differentiation at approximately the same stage as the CD19 antigen.CD22 membrane expression is limited to the late differentiation stages. There are twodifferent isoforms of CD22. It exists as a monomer and heterodimer. CD22 is stronglyexpressed in hairy cell leukemia.CD20 Rabbit PolyclonalCatalog No. Size FormatE2560 0.1ml ConcentrateE2562 0.5ml ConcentrateE2564 1.0ml ConcentrateE2561 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide fromC-terminus of human CD20 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: B Cell Lymphoma, TonsilCellular Localization: Cytoplasm,MembraneControl Lysate: RajiMolecular Weight: 35 kDaCD23 Rabbit MonoclonalCatalog No. Size FormatM3230 0.1ml ConcentrateM3232 0.5ml ConcentrateM3234 1.0ml ConcentrateM3231 7.0ml Ready-to-useClone: SP23Immunogen: Recombinant protein encodingaa 48-248 of human CD23.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneControl Lysate: RajiMolecular Weight: 45 kDaHuman Tonsil stained withanti-CD20 antibody E256Human Tonsil stained withanti-CD23 antibody M323Product Description: CD20 is a leukocyte surface antigen consisting of fourtransmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarilyon B cells but has also been detected on both normal and neoplastic T cells. Activationof CD20 significantly increases DNA synthesis and is thought to involve basic helix-loophelixleucine zipper transcription factors.Product Description: CD23 is a 45kDa glycoprotein, which is present on a subpopulationof freshly isolated peripheral blood and tonsil B cells and strongly expressed onEBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinityIgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cellsfrom cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/centrocytic lymphoma.28Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCD23 Rabbit PolyclonalCD31 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE3020 0.1ml ConcentrateE3022 0.5ml ConcentrateImmunogen: Recombinant protein encoding aa48-248 of human CD23.E11110 0.1ml ConcentrateE11112 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of mouse CD31 protein.E3024 1.0ml ConcentrateApplication: IHC-PE11114 1.0ml ConcentrateApplication: IHC-PE3021 7.0ml Ready-to-useSpecies Reactivity: Human*E11111 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: TonsilCellular Localization: MembraneCellular Localization: MembraneHuman Tonsil stained withanti-CD23 antibody E302Human Tonsil stained withanti-CD31 antibody E1111Product Description: CD23 is a 45kDa glycoprotein, which is present on a subpopulationof freshly isolated peripheral blood and tonsil B cells and strongly expressed onEBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinityIgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cellsfrom cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/centrocytic lymphoma.Product Description: CD31 is a glycoprotein expressed on endothelial cells and inplatelets. It is known to be involved in cell signaling and cell adhesion.CD30 Rabbit PolyclonalCatalog No. Size FormatE17410 0.1ml ConcentrateE17412 0.5ml ConcentrateE17414 1.0ml ConcentrateE17411 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from internalregion of human CD30.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s Lymphoma, TonsilCellular Localization: MembraneCD34 (Endothelial Cell Marker) Rabbit PolyclonalCatalog No. Size FormatE17540 0.1ml ConcentrateE17542 0.5ml ConcentrateE17544 1.0ml ConcentrateE17541 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tohuman CD34 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Angiosarcoma, Lung, TonsilCellular Localization: Cytoplasm, MembraneHuman Hodgkin’s Lymphoma stained withanti-CD30 antibody E1741Human Angiosarcoma stained withanti-CD34 (Endothelial Cell Marker) antibody E1754Product Description: CD30, a single chain glycoprotein, is synthesized as a 90kDaprecursor which is processed in the Golgi complex into a membrane-boundphosphorylated mature 105/120kDa glycoprotein. The CD30/Ki-1 antigen is expressedby mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin’s disease,by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varyingproportion of activated T and B cells.Product Description: CD34, a single chain transmembrane glycoprotein, is selectivelyexpressed on human lymphoid and myeloid hematopoietic progenitor cells.CD31 Rabbit MonoclonalCatalog No. Size FormatM3380 0.1ml ConcentrateM3382 0.5ml ConcentrateM3384 1.0ml ConcentrateM3381 7.0ml Ready-to-useClone: SP38Immunogen: Synthetic peptide corresponding toC-terminus of mouse CD31 protein.Application: IHC-PSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD34 (Endothelial Cell Marker) Mouse MonoclonalCatalog No. Size FormatE1280 0.1ml ConcentrateE1282 0.5ml ConcentrateE1284 1.0ml ConcentrateE1281 7.0ml Ready-to-useClone: QBEnd/10Immunogen: Detergent solubilized vesicularsuspension prepared from a perfusate of humanterm placenta.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Lung, TonsilCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-CD31 antibody M338Human Tonsil stained withanti-CD34 (Endothelial Cell Marker) antibody E128Product Description: CD31 is a glycoprotein expressed on endothelial cells and inplatelets. It is known to be involved in cell signaling and cell adhesion.Product Description: CD34, a single chain transmembrane glycoprotein, is selectivelyexpressed on human lymphoid and myeloid hematopoietic progenitor cells.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 29

CD38 Rabbit PolyclonalCD43 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE19170 0.1ml ConcentrateE19172 0.5ml ConcentrateImmunogen: Synthetic peptide near theC-terminus of human CD38.E17950 0.1ml ConcentrateE17952 0.5ml ConcentrateImmunogen: Synthetic peptide from internal region ofhuman CD43 protein.E19174 1.0ml ConcentrateApplication: IHC-P, WBE17954 1.0ml ConcentrateApplication: IHC-PE19171 7.0ml Ready-to-useSpecies Reactivity: Human*E17951 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: TonsilCellular Localization: Cytoplasm,MembraneCellular Localization: MembraneControl Lysate: HeLaMolecular Weight: 46 kDa (predicted)Human Tonsil stained withanti-CD38 antibody E1917Human Tonsil stained withanti-CD43 antibody E1795Product Description: CD38 (cluster of differentiation 38), also known as cyclic ADPribose hydrolase, is a transmembrane glycoprotein found on the surface of manyimmune cells including plasma cells, activated or immature T and B cells, monocytes,and natural killer cells. CD38 participates in cell adhesion, signal transduction andcalcium signaling. CD38 is used as one of the plasma cell markers and its ligand isCD31 molecules.Product Description: CD43 is one of the major glycoproteins of thymocytes and Tlymphocytes. It plays a role in the physiochemical properties of the T cell surface andin lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extendedrod-like structure that could protrude above the glycocalyx of the cell and allow multipleglycan chains to be accessible for binding. During T cell activation CD43 is activelyremoved from the T cell antigen presenting cell contact site.CD40 Rabbit PolyclonalCatalog No. Size FormatE3700 0.1ml ConcentrateE3702 0.5ml ConcentrateE3704 1.0ml ConcentrateE3701 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human CD40.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s Lymphoma, TonsilCellular Localization: MembraneCD44 Std** Rabbit MonoclonalCatalog No. Size FormatM3370 0.1ml ConcentrateM3372 0.5ml ConcentrateM3374 1.0ml ConcentrateM3371 7.0ml Ready-to-useClone: SP37Immunogen: Synthetic peptidecorresponding to human CD44.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Esophageal CarcinomaCellular Localization: MembraneControl Lysate: HeLaMolecular Weight: 80 kDaHuman Hodgkin’s Lymphoma stained withanti-CD40 antibody E370Human Esophageal Carcinoma stained withanti-CD44 Std antibody M337Product Description: The CD40 antigen is a single chain glycoprotein. It is known to bea member of the tumor necrosis factor/nerve growth factor superfamily and shows asignificant homology to the Hodgkin’s disease associated antigen, CD30. CD40 is presenton all B cells except plasma cells. It is also found on some epithelial cells, carcinomas andlymphoid dentritic cells.Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes,monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule(H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 hasbeen implicated in cell migration, in lymphocyte homing, and in tumor metastasis.CD43 Rabbit MonoclonalCD44 Std Rabbit PolyclonalCatalog No. Size FormatClone: SP55Catalog No. Size FormatClone: N/AM3550 0.1ml ConcentrateM3552 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromthe human CD43.E17370 0.1ml ConcentrateE17372 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human CD44.M3554 1.0ml ConcentrateApplication: IHC-P, WBE17374 1.0ml ConcentrateApplication: IHC-PM3551 7.0ml Ready-to-useSpecies Reactivity: Human*E17371 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Esophageal CarcinomaCellular Localization: MembraneCellular Localization: MembraneControl Lysate: JurkatMolecular Weight: 95/115/135 kDaHuman Tonsil stained withanti-CD43 antibody M355Human Esophageal Carcinoma stained withanti-CD44 Std antibody E1737Product Description: CD43 is one of the major glycoproteins of thymocytes and Tlymphocytes. It plays a role in the physiochemical properties of the T cell surface andin lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extendedrodlike structure that could protrude above the glycocalyx of the cell and allow multipleglycan chains to be accessible for binding. During T cell activation CD43 is activelyremoved from the T cell antigen presenting cell contact site.Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes,monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule(H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 hasbeen implicated in cell migration, in lymphocyte homing, and in tumor metastasis.30Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCD44 Std Mouse MonoclonalCD56 Mouse MonoclonalCatalog No. Size FormatClone: SPM521Catalog No. Size FormatClone: SPM128E17360 0.1ml ConcentrateE17362 0.5ml ConcentrateImmunogen: Synthetic peptidecorresponding to human CD44.E1370 0.1ml ConcentrateE1372 0.5ml ConcentrateImmunogen: Membrane preparation of a small celllung carcinoma.E17364 1.0ml ConcentrateApplication: IHC-P, WBE1374 1.0ml ConcentrateApplication: IHC-PE17361 7.0ml Ready-to-useSpecies Reactivity: Human*E1371 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Mouse IgG2aIsotype: Mouse IgG1/kControl Tissue: Esophageal CarcinomaControl Tissue: Brain, NeuroblastomaCellular Localization: MembraneCellular Localization: MembraneControl Lysate: HeLaMolecular Weight: 80 kDaHuman Esophageal Carcinoma stained withanti-CD44 Std antibody E1736Human Neuroblastoma stained withanti-CD56 antibody E137Product Description: CD44 is a cell surface glycoprotein expressed on lymphocytes,monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule(H-CAM), phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 hasbeen implicated in cell migration, in lymphocyte homing, and in tumor metastasis.Product Description: Three isoforms of neural cell adhesion molecule (NCAM) areproduced by differential splicing of the RNA transcript from a single gene. NCAM(CD56) is reported to express on most neuroectodermal derived cell lines, tissues, andneoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma.It is also expressed on some mesodermally derived tumors such as rhabdomyosarcomaand also on natural killer cells.CD45/T200/LCA Mouse MonoclonalClone: SPM496Catalog No. Size FormatE12430 0.1ml Concentrate Immunogen: Stimulated human leucocytes.E12432 0.5ml Concentrate Application: IHC-PE12434 1.0ml ConcentrateSpecies Reactivity: Human*E12431 7.0ml Ready-to-useIsotype: Mouse IgG1/kControl Tissue: TonsilCellular Localization: MembraneCD56 Mouse MonoclonalCatalog No. Size FormatE11100 0.1ml ConcentrateE11102 0.5ml ConcentrateE11104 1.0ml ConcentrateE11101 7.0ml Ready-to-useClone: 123C3Immunogen: Membrane preparation of a small celllung carcinoma.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Brain, NeuroblastomaCellular Localization: MembraneHuman Tonsil stained withanti-CD45/T200/LCA antibody E1243Human Neuroblastoma stained withanti-CD56 antibody E1110Product Description: CD45 leucocyte common antigen (LCA) belongs to a family of atleast four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed onhematopoietic cell lines but absent on non-hematopoietic cell lines and normal andmalignant non-hematopoietic tissues. The intracellular portion of these molecules hasprotein phosphatase activity and is involved in regulation of transmembrane signals.Product Description: Three isoforms of neural cell adhesion molecule (NCAM) areproduced by differential splicing of the RNA transcript from a single gene. NCAM(CD56) is reported to express on most neuroectodermal derived cell lines, tissues, andneoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma.It is also expressed on some mesodermally derived tumors such as rhabdomyosarcomaand also on natural killer cells.CD45RA Mouse MonoclonalCD63 Mouse MonoclonalCatalog No. Size FormatClone: SPM504Catalog No. Size FormatClone: SPM524E16480 0.1ml ConcentrateImmunogen: Stimulated human leucocytes.E17510 0.1ml ConcentrateImmunogen: Recombinant human CD63 protein.E16482 0.5ml ConcentrateE16484 1.0ml ConcentrateE16481 7.0ml Ready-to-useApplication: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG2aE17512 0.5ml ConcentrateE17514 1.0ml ConcentrateE17511 7.0ml Ready-to-useApplication: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: TonsilControl Tissue: MelanomaCellular Localization: MembraneCellular Localization: CytoplasmHuman Tonsil stained withanti-CD45RA antibody E1648Human Melanoma stained withanti-CD63 antibody E1751Product Description: CD45 or Leukocyte Common Antigen (LCA/T200) is a membranesialoglycoprotein. The differential splicing of the exons generates various isoforms.CD45RA is a single chain glycoprotein. It is expressed on appoximately 40-50% ofperipheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines.T-cells expressing CD45RA are naive T-cells while CD45RO+ cells are memory T-cells.Product Description: CD63 is expressed on activated platelets. CD63 is a lysosomalmembrane glycoprotein that is translocated to plasma membrane after plateletactivation. It is also present in monocytes and macrophages and is weakly expressed ongranulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen whichis ME491 and to the platelet antigen PTLGP40.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 31

CD68 Rabbit PolyclonalCatalog No. Size FormatE19190 0.1ml ConcentrateE19192 0.5ml ConcentrateE19194 1.0ml ConcentrateE19191 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide in the internal region ofhuman CD68 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmCD74 Mouse MonoclonalCatalog No. Size FormatE17420 0.1ml ConcentrateE17422 0.5ml ConcentrateE17424 1.0ml ConcentrateE17421 7.0ml Ready-to-useClone: SPM523Immunogen: Recombinant human CD74protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: TonsilCellular Localization: MembraneControl Lysate: RamosMolecular Weight: 35 kDaHuman Tonsil stained withanti-CD68 antibody E1919Human Tonsil stained withanti-CD74 antibody E1742Product Description: CD68 is an intracellular glycoprotein mainly expressed onmacrophages and monocytes. It could play a role in phagocytic activities of tissuemacrophages, both in intracellular lysosomal metabolism and extracellular cell-cell andcell-pathogen interactions. CD68 is also expressed by interdigitating reticulum cells intonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML),and granulocytic sarcoma.Product Description: Expression of the invariant chain of the HLA-DR antigen (CD74)by B-lymphocytes begins in the pre-B stage but lost before the final differentiation toplasma cells. This antibody is expressed in B-lymphocytes and many B cell lymphomas.CD68 Rabbit PolyclonalCatalog No. Size FormatE19160 0.1ml ConcentrateE19162 0.5ml ConcentrateE19164 1.0ml ConcentrateE19161 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide near theC-terminus of human CD68 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmControl Lysate: Raji, SH-SY5YMolecular Weight: 37 kDa Protein(calculated); 110 kDa (apparent)CD79a Rabbit MonoclonalCatalog No. Size FormatM3180 0.1ml ConcentrateM3182 0.5ml ConcentrateM3184 1.0ml ConcentrateM3181 7.0ml Ready-to-useClone: SP18Immunogen: Synthetic peptide derived fromN-terminus region of human CD79a protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: B Cell Lymphoma, TonsilCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-CD68 antibody E1916Human B Cell Lymphoma stained withanti-CD79a antibody M318Product Description: CD68 is an intracellular glycoprotein mainly expressed onmacrophages and monocytes. It could play a role in phagocytic activities of tissuemacrophages, both in intracellular lysosomal metabolism and extracellular cell-cell andcell-pathogen interactions. CD68 is also expressed by interdigitating reticulum cells intonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML),and granulocytic sarcoma.Product Description: A disulphide-linked heterodimer, consisting of CD79a/mb-1 andCD79b/B29 polypeptides, CD79a is non-covalently associated with membrane-boundimmunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appearsat pre B cell stage and persists until the plasma cell stage where it is found as anintracellular component. CD79a is found in the majority of acute leukemias of precursorB cell type, in B cell lines, B cell lymphomas, and in some myelomas.CD68 Rabbit PolyclonalCatalog No. Size FormatE13920 0.1ml ConcentrateE13922 0.5ml ConcentrateE13924 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Recombinant proteinencoding aa 155-355 of human CD68.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: Human BrainMolecular Weight: 38 kDaCD79a Rabbit PolyclonalCatalog No. Size FormatE2490 0.1ml ConcentrateE2492 0.5ml ConcentrateE2494 1.0ml ConcentrateE2491 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derivedfrom N-terminus of human CD79a protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: B Cell Lymphoma, TonsilCellular Localization: MembraneControl Lysate: RajiMolecular Weight: 44-48 kDaN/A E1392Human Tonsil stained withanti-CD79a antibody E249Product Description: CD68 is an intracellular glycoprotein expressed on macrophagesand monocytes. CD68 expression has been demonstrated in T cells, NK cells, liver andrenal tubules.Product Description: A disulphide-linked heterodimer, consisting of CD79a/mb-1 andCD79b/B29 polypeptides, CD79a is non-covalently associated with membrane-boundimmunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appearsat pre B cell stage and persists until the plasma cell stage where it is found as anintracellular component. CD79a is found in the majority of acute leukemias of precursorB cell type, in B cell lines, B cell lymphomas, and in some myelomas.32Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCD95/FAS Rabbit PolyclonalCatalog No. Size FormatE2500 0.1ml ConcentrateE2502 0.5ml ConcentrateE2504 1.0ml ConcentrateE2501 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human FAS protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Small IntestineCellular Localization: MembraneCD105/Endoglin/TGF 1/3 Receptor Rabbit PolyclonalCatalog No. Size FormatE4230 0.1ml ConcentrateE4232 0.5ml ConcentrateE4234 1.0ml ConcentrateE4231 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant protein encoding 26-176 aaof human CD105.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneHuman Colon stained withanti-CD95/FAS antibody E250Human Tonsil stained withanti-CD105/Endoglin/TGF 1/3 Receptor antibody E423Product Description: CD95, also known as FAS, is a transmembrane glycoprotein. It is amember of the nerve growth factor receptor/tumor necrosis factor superfamily. This cellsurface molecule mediates receptor-triggered apoptosis. The FAS antigen is expressedon the surface of various cell types, including activated T and B lymphocytes andT lymphoblastoid cell lines.Product Description: CD105 or endoglin or GP160 is a Type I transmembraneprotein, which is highly expressed on human vascular endothelial cells. It exists as anO- and N-glycosylated homodimer. Up-regulation of endoglin expression has beendemonstrated in tumor vasculature and proliferating cells, suggesting that it is aproliferation-associated endothelial cell marker. CD105 binds to TGF-Beta1 and Beta3with high affinity but not TGF-Beta2.CD99 Rabbit MonoclonalCatalog No. Size FormatM4190 0.1ml ConcentrateM4192 0.5ml ConcentrateM4194 1.0ml ConcentrateM4191 7.0ml Ready-to-useClone: SP119Immunogen: Recombinant full length humanCD99 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: Cytoplasm, MembraneCD117/c-kit Rabbit MonoclonalCatalog No. Size Format Clone: SP26M3260 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toM3262 0.5ml Concentrate cytoplasmic domain of human CD117 protein.M3264 1.0ml Concentrate Application: IHC-PM3261 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Gastrointestinal Stromal TumorCellular Localization: CytoplasmHuman Pancreas stained withanti-CD99 antibody M419Human Gastrointestinal Stromal Tumor stained withanti-CD117/c-kit antibody M326Product Description: CD99, or MIC2 gene product, or E2 antigen is expressed on the cellmembrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary.The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis,and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2is strongly expressed on Ewing’s sarcoma cells and primitive peripheralneuroectodermal tumors.Product Description: CD117, also called KIT or c-kit receptor, is a cytokine receptoron the surface of hematopoietic stem cells and other cells. CD117 gene encodes thehuman homolog of the proto-oncogene c-kit. Mutations in this gene are associated withgastrointestinal stromal tumors, mast cell disease, and chronic myelogenous leukemia.CD99/MIC2 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3390 0.1ml Concentrate Immunogen: Recombinant full lengthE3392 0.5ml Concentrate human CD99 protein.E3394 1.0ml Concentrate Application: IHC-P, WBE3391 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: Cytoplasm,MembraneControl Lysate: JurkatMolecular Weight: 32/28 kDaHuman Pancreas stained withanti-CD99/MIC2 antibody E339CD117/c-kit Rabbit PolyclonalCatalog No. Size Format Clone: N/AE1440 0.1ml Concentrate Immunogen: Synthetic peptide from the cytoplasmicE1442 0.5ml Concentrate domain of human CD117/c-kit protein.E1444 1.0ml Concentrate Application: IHC-PE1441 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Gastrointestinal Stromal TumorCellular Localization: CytoplasmHuman Gastrointestinal Stromal Tumor stained withanti-CD117/c-kit antibody E144Product Description: CD99, or MIC2 gene product, or E2 antigen is expressed on the cellmembrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary.The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis,and some endothelial cells. Mature granulocytes express very little or no CD99.MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheralneuroectodermal tumors.Product Description: CD117, also called KIT or c-kit receptor, is a cytokine receptoron the surface of hematopoietic stem cells and other cells. CD117 gene encodes thehuman homolog of the proto-oncogene c-kit. Mutations in this gene are associated withgastrointestinal stromal tumors, mast cell disease, and chronic myelogenous leukemia.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 33

CD138 Rabbit PolyclonalCatalog No. Size FormatE4560 0.1ml ConcentrateE4562 0.5ml ConcentrateE4564 1.0ml ConcentrateE4561 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant protein encoding aa 18-218human CD138.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneCD163 Rabbit MonoclonalCatalog No. Size FormatM3960 0.1ml ConcentrateM3962 0.5ml ConcentrateM3964 1.0ml ConcentrateM3961 7.0ml Ready-to-useClone: SP96Immunogen: Synthetic peptide corresponding to nearN-terminus of human CD163 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: MembraneHuman Tonsil stained withanti-CD138 antibody E456Human Lung stained withanti-CD163 antibody M396Product Description: CD138/Syndecan-1 is a transmembrane heparin sulphateproteoglycan which is made up of one core protein and five glycosaminoglycan. CD138is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells andplasma cells but is absent from mature B cells. It is lost from the apoptotic myeloma cells.Product Description: CD163, a 130kDa membrane glycoprotein, is expressed exclusivelyon the cell surface of human monocytes and macrophages that evolve predominantly inthe late phase of inflammation. CD163 is present on all CD14 positive monocytes, mostCD64 positive monocytes, and shows higher expression on CD16 positive monocytes.CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone.CD146 Rabbit PolyclonalCatalog No. Size FormatE11130 0.1ml ConcentrateE11132 0.5ml ConcentrateE11134 1.0ml ConcentrateE11131 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant human CD146 proteinencoding aa 433-647 expressed in E. coli.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AngiosarcomaCellular Localization: MembraneCD163 Rabbit PolyclonalCatalog No. Size FormatE18680 0.1ml ConcentrateE18682 0.5ml ConcentrateE18684 1.0ml ConcentrateE18681 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from nearN-terminus of human CD163 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Skin, ThymusCellular Localization: MembraneHuman Angiosarcoma stained withanti-CD146 antibody E1113Human Skin stained withanti-CD163 antibody E1868Product Description: CD146, also known as the melanoma cell adhesion molecule(MCAM), is a 113kDa cell adhesion molecule currently used as a marker for endothelialcell lineage. CD146 belongs to the immunoglobulin super-gene family with fiveimmunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residuecytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+independent cell adhesion molecule involved in heterophilic cell to cell interactions.Product Description: CD163, a 130kDa membrane glycoprotein, is expressed exclusivelyon the cell surface of human monocytes and macrophages that evolve predominantly inthe late phase of inflammation. CD163 is present on all CD14 positive monocytes, mostCD64 positive monocytes, and shows higher expression on CD16 positive monocytes.CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone.CD150 Rabbit PolyclonalCatalog No. Size FormatE13940 0.1ml ConcentrateE13942 0.5ml ConcentrateE13944 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Recombinant proteinencoding aa 1-136 of human CD150.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: Human HeartMolecular Weight: 37 kDaCD168 Rabbit PolyclonalCatalog No. Size FormatE18740 0.1ml ConcentrateE18742 0.5ml ConcentrateE18744 1.0ml ConcentrateE18741 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from nearC-terminus of human CD168 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: MembraneN/A E1394Human Prostate Carcinoma stained withanti-CD168 antibody E1874Product Description: CD150, designated SLAM (Signaling Lymphocyte ActivationMolecule) or CDw150, belongs to the immunoglobulin gene superfamily and is involvedin T cell stimulation. SLAM is constitutively expressed on peripheral blood memoryT cells, immature thymocytes, and a proportion of B cells, and is rapidly induced onnaive T cells after activation. Activated B cells express the membrane bound form, andthe cytoplasmic isoforms of SLAM.Product Description: The CD168 antigen is a non-integral cell surface hyaluronanreceptor and intracellular protein. When hyaluronan binds to CD168, thephosphorylation of a number of proteins, including the focal adhesion kinaseoccurs. CD168 activation regulates various cellular processes including cellmigration, proliferation, signaling, regulation of gene expression, cell differentiation,morphogenesis, and metastasis.34Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

CDK2 Rabbit PolyclonalCEA/CD66e Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18540 0.1ml ConcentrateE18542 0.5ml ConcentrateE18544 1.0ml ConcentrateE18541 7.0ml Ready-to-useImmunogen: Synthetic peptide derivedfrom C-terminus of human CDK2 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, NucleusE2970 0.1ml ConcentrateE2972 0.5ml ConcentrateE2974 1.0ml ConcentrateE2971 7.0ml Ready-to-useImmunogen: Purified human CEA.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, Luminal MembraneControl Lysate: HeLaMolecular Weight: 33 kDaHuman Tonsil stained withanti-CDK2 antibody E1854Human Colon Carcinoma stained withanti-CEA/CD66e antibody E297Product Description: CDK2 is a member of the Ser/Thr protein kinase family. It is highlysimilar to the gene products of S. cerevisiae CDC28, and S. pombe CDC2. It is a catalyticsubunit of the cyclin-dependent protein kinase complex, whose activity is restrictedto the G1-S phase, and is essential for cell cycle G1/S phase transition. This proteinis regulated by the regulatory subunits of the complex including cyclin A or E, CDKinhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).Product Description: Carcinoembryonic antigen (CEA), is synthesized duringdevelopment in the fetal gut, and is re-expressed in increased amounts in intestinalcarcinomas and several other tumors. CEA is expressed in various metastaticadenocarcinomas and in pulmonary adenocarcinomas.CDX2 Rabbit MonoclonalCatalog No. Size FormatM3540 0.1ml ConcentrateM3542 0.5ml ConcentrateM3544 1.0ml ConcentrateM3541 7.0ml Ready-to-useClone: SP54Immunogen: Synthetic peptide corresponding toN-terminus of human CDX2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusCEA/CD66e Mouse MonoclonalCatalog No. Size Format Clone: SPM506E16500 0.1ml Concentrate Immunogen: Purified human CEA.E16502 0.5ml Concentrate Application: IHC-PE16504 1.0ml ConcentrateSpecies Reactivity: Human*E16501 7.0ml Ready-to-useIsotype: Mouse IgG1Control Tissue: Colon CarcinomaCellular Localization: Cytoplasm, Luminal MembraneHuman Colon Carcinoma stained withanti-CDX2 antibody M354Human Colon Carcinoma stained withanti-CEA/CD66e antibody E1650Product Description: CDX2 encodes an intestine-specific transcription factor that isexpressed early in intestinal development and may be involved in the regulation ofproliferation and differentiation of intestinal epithelial cells. It is expressed in the nucleiof epithelial cells throughout the intestine, colorectal carcinomas, intestinal metaplasiaof the stomach, and intestinal-type gastric cancer, while it is not expressed in the normalgastric mucosa.Product Description: Carcinoembryonic antigen (CEA), is synthesized duringdevelopment in the fetal gut, and is re-expressed in increased amounts in intestinalcarcinomas and several other tumors. CEA is expressed in various metastaticadenocarcinomas and in pulmonary adenocarcinomas.CDX2 Rabbit PolyclonalCatalog No. Size FormatE14180 0.1ml ConcentrateE14182 0.5ml ConcentrateE14184 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Synthetic peptidecorresponding to N-terminus of humanCDX2.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: Human PlacentaMolecular Weight: 37 kDaN/A E1418c-erbB-2 (Paired 1248) Rabbit PolyclonalCatalog No. Size FormatE18480 0.1ml ConcentrateE18482 0.5ml ConcentrateE18484 1.0ml ConcentrateE18481 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from the regionof human c-erbB-2 that contains non-phosphorylatedtyrosine 1248.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-erbB-2 (Paired 1248) antibody E1848Product Description: CDX2 encodes an intestine-specific transcription factor that isexpressed early in intestinal development and may be involved in the regulation ofproliferation and differentiation of intestinal epithelial cells. It is expressed in the nucleiof epithelial cells throughout the intestine, colorectal carcinomas, intestinal metaplasiaof the stomach, and intestinal-type gastric cancer, while it is not expressed in the normalgastric mucosa.Product Description: c-erbB-2 is a 185kDa transmembrane tyrosine kinase that belongsto the epidermal growth factor receptor (EGFR) family. This family comprises fourhomologous receptors: ERFR, c-erbB-2, cerbB-3 and c-erbB-4. Activation of c-erbB-2through autophosphorylation at tyrosine residues, is initiated by either homo- orheterodimerization and triggers intracellular signaling events. This antibody labelsc-cerbB-2 that is non-phosphorylated at 1248 tyrosine residue.36Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary Antibodiesc-erbB-2 (Phospho Y1248) Rabbit PolyclonalCatalog No. Size FormatE18470 0.1ml ConcentrateE18472 0.5ml ConcentrateE18474 1.0ml ConcentrateE18471 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from theregion of human c-erbB-2 that contains phosphorylatedtyrosine 1248.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Membranec-erbB-3/HER-3 Rabbit MonoclonalCatalog No. Size Format Clone: SP71M3710 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toM3712 0.5ml Concentrate internal region of human c-erbB-3.M3714 1.0ml Concentrate Application: IHC-PM3711 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Small IntestineCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-erbB-2 (Phospho Y1248) antibody E1847Human Small Intestine stained withanti-c-erbB-3/HER-3 antibody M371Product Description: c-erbB-2 is a 185kDa transmembrane tyrosine kinase belongsto the epidermal growth factor receptor (EGFR) family. This family comprises fourhomologous receptors: ERFR, c-erbB-2, cerbB-3 and c-erbB-4. Activation of c-erbB-2through autophosphorylation at tyrosine residues, is initiated by either homo- orheterodimerization and triggers intracellular signaling events. This antibody labelsc-cerbB-2 that is non-phosphorylated at 1248 tyrosine residue.Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of humanchromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translatedinto a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as wellas transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 isoverexpressed in a variety of tumors including breast, stomach, pancreas, and colon.c-erbB-2/HER-2 Rabbit MonoclonalCatalog No. Size Format Clone: SP3M3030 0.1ml Concentrate Immunogen: Recombinant protein encodingM3032 0.5ml Concentrate extracellular domain of human c-erbB-2.M3034 1.0ml Concentrate Application: IHC-PM3031 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Membranec-erbB-3/HER-3 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18580 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE18582 0.5ml Concentrate region of human c-erbB-3 protein.E18584 1.0ml Concentrate Application: IHC-PE18581 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Colon CarcinomaCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-erbB-2/HER-2 antibody M303Human Colon Carcinoma stained withanti-c-erbB-3/HER-3 antibody E1858Product Description: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family.It is closely related in structure to the epidermal growth factor receptor. c-erbB-2oncoprotein is detectable in a proportion of breast and other adenocarconomas,as well as transitional cell carcinomas.Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of humanchromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translatedinto a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as wellas transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 isoverexpressed in a variety of tumors including breast, stomach, pancreas, and colon.c-erbB-2/HER-2 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2440 0.1ml Concentrate Immunogen: Synthetic peptide from C-terminus ofE2442 0.5ml Concentrate human c-erbB-2 protein.E2444 1.0ml Concentrate Application: IHC-PE2441 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Membranec-erbB-3/HER-3 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17930 0.1ml Concentrate Immunogen: Synthetic peptide from near N-terminusE17932 0.5ml Concentrate of human c-erbB-3 protein.E17934 1.0ml Concentrate Application: IHC-PE17931 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Colon CarcinomaCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-c-erbB-2/HER-2 antibody E244Human Breast Carcinoma stained withanti-c-erbB-3/HER-3 antibody E1793Product Description: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family.It is closely related in structure to the epidermal growth factor receptor. c-erbB-2oncoprotein is detectable in a proportion of breast and other adenocarconomas,as well as transitional cell carcinomas.Product Description: The gene for c-erbB-3/HER-3 is located on the long arm of humanchromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translatedinto a 160/180kDa glycoprotein. The c-erbB-3/HER-3 gene encodes secreted as wellas transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB-3 isoverexpressed in a variety of tumors including breast, stomach, pancreas, and colon.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 37

c-erbB-4/HER-4 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2760 0.1ml Concentrate Immunogen: Synthetic peptide fromE2762 0.5ml Concentrate C-terminus of human c-erbB-4 protein.E2764 1.0ml Concentrate Application: IHC-P, WBE2761 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm,MembraneControl Lysate: A431Molecular Weight: 140 kDaHuman Breast Carcinoma stained withanti-c-erbB-4/HER-4 antibody E276Chromogranin A Rabbit MonoclonalCatalog No. Size FormatM3120 0.1ml ConcentrateM3122 0.5ml ConcentrateM3124 1.0ml ConcentrateM3121 7.0ml Ready-to-useClone: SP12Immunogen: Recombinant protein encoding humanchromogranin A.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmHuman Pancreas stained withanti-Chromogranin A antibody M312Product Description: c-erbB-4 or HER-4 is the fourth member of the class I receptorkinase family. HER-4 is expressed in normal skeletal muscle, heart, pituitary, cerebellum,and breast. It is specifically bound and activated by neuregulins, NRG-2, NRG-3, heparinbindingEGF-like growth factor, betacellulin and NTAK. Interaction with these factorsinduces cell differentiation. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 andH3396 are found to have the highest levels of mRNA.Product Description: Chromogranin A (a protein of 439 amino acid which is encoded onchromosome 14) is present in neuroendocrine cells throughout the body, including theneuroendocrine cells of the large and small intestine, adrenal medulla and pancreaticislets. It has been identified in carcinoid tumors, phenochromocytomas, paragangliomas,and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specificenolase (NSE) is common in neuroendocrine neoplasms.c-Fos Rabbit PolyclonalCatalog No. Size FormatE4460 0.1ml ConcentrateE4462 0.5ml ConcentrateE4464 1.0ml ConcentrateE4461 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived from internalregion of human c-Fos oncoprotein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SkinCellular Localization: NucleusChromogranin A Rabbit PolyclonalCatalog No. Size FormatE1520 0.1ml ConcentrateE1522 0.5ml ConcentrateE1524 1.0ml ConcentrateE1521 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant protein encoding humanchromogranin A.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmHuman Skin stained withanti-c-Fos antibody E446Human Pancreas stained withanti-Chromogranin A antibody E152Product Description: The c-Fos proto-oncogene encodes a nuclear phosphoproteinand is a regulator of transcription, forming a complex with the c-Jun proto-oncogeneproduct. Expression of the c-Fos gene is low in most normal adult tissues. However,high levels of expression have been detected in normal skin.Product Description: Chromogranin A (a protein of 439 amino acid which is encoded onchromosome 14) is present in neuroendocrine cells throughout the body, including theneuroendocrine cells of the large and small intestine, adrenal medulla and pancreaticislets. It has been identified in carcinoid tumors, phenochromocytomas, paragangliomas,and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specificenolase (NSE) is common in neuroendocrine neoplasms.Cholecystokinin (CCK-8) Rabbit PolyclonalCatalog No. Size FormatE4510 0.1ml ConcentrateE4512 0.5ml ConcentrateE4514 1.0ml ConcentrateE4511 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide sulfatedcholecystokinin (26-33) amide(sulfated CCK-8).Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: CytoplasmControl Lysate: Human BrainMolecular Weight: 12 kDaHuman Stomach stained withanti-Cholecystokinin (CCK-8) antibody E451Claudin-1 Rabbit PolyclonalCatalog No. Size FormatE3410 0.1ml ConcentrateE3412 0.5ml ConcentrateE3414 1.0ml ConcentrateE3411 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human claudin-1 peptide.Application: IHC-P, WBSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, SkinCellular Localization: Cell Junction,Cytoplasm, MembraneControl Lysate: HeLaMolecular Weight: 20 kDaHuman Skin stained withanti-Claudin-1 antibody E341Product Description: Cholecystokinin (CCK) is a neuropeptide hormone andneurotransmitter widely distributed throughout the gastrointestinal (GI) tract and thecentral nervous system (CNS). Antibodies that react specifically with CCK are usefulfor the study of the mode of action, differential tissue expression and intracellular andsubcellular localization of CCK in the CNS and periphery as well as in neuroendocrinecells of the digestive system.Product Description: Claudin 1-18 proteins are a family of transmembrane proteinsassociated with tight junctions. Tight junctions are specialized regions of cell to cellcontact, made up of a network of strands to act as a molecular gasket for preventing theleakage of ions and water between cells. They are abundant in luminal epithelial sheetswhere they maintain epithelial cell polarity. Different tissues exhibit different claudincomposition.38Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesClaudin-3 Rabbit PolyclonalCatalog No. Size FormatE3830 0.1ml ConcentrateE3832 0.5ml ConcentrateE3834 1.0ml ConcentrateE3831 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of mouse claudin-3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Small IntestineCellular Localization: Cytoplasm, MembraneClaudin-7 Rabbit PolyclonalCatalog No. Size FormatE10590 0.1ml ConcentrateE10592 0.5ml ConcentrateE10594 1.0ml ConcentrateE10591 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman claudin-7 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, MembraneHuman Small Intestine stained withanti-Claudin-3 antibody E383Human Colon Carcinoma stained withanti-Claudin-7 antibody E1059Product Description: Claudin 1-18 proteins are a family of transmembrane proteinsassociated with tight junctions. Tight junctions are specialized regions of cell to cellcontact, made up of a network of strands to act as a molecular gasket for preventing theleakage of ions and water between cells. They are abundant in luminal epithelial sheetswhere they maintain epithelial cell polarity. Different tissues exhibit different claudincomposition.Product Description: The claudin superfamily consists of many structurally relatedproteins in humans. These proteins, which include claudin 1 through 18, are importantstructural and functional components of tight junctions in paracellular transport.Claudins are located in both epithelial and endothelial cells in all tight junction-bearingtissues. Claudins, which consist of four trans-membrane domains and two extracellularloops, make up tight junction strands.Claudin-4 Rabbit PolyclonalCatalog No. Size FormatE3980 0.1ml ConcentrateE3982 0.5ml ConcentrateE3984 1.0ml ConcentrateE3981 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman claudin-4.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Ovarian Carcinoma, TonsilCellular Localization: Cytoplasm, MembraneClaudin-10 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10240 0.1ml Concentrate Immunogen: Synthetic peptide from C-terminus ofE10242 0.5ml Concentrate human claudin-10 protein.E10244 1.0ml Concentrate Application: IHC-PE10241 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain, Skeletal MuscleCellular Localization: CytoplasmHuman Ovarian Carcinoma stained withanti-Claudin-4 antibody E398Human Skeletal Muscle stained withanti-Claudin-10 antibody E1024Product Description: Claudin 1-18 proteins are a family of transmembrane proteinsassociated with tight junctions. Tight junctions are specialized regions of cell to cellcontact, made up of a network of strands to act as a molecular gasket for preventing theleakage of ions and water between cells. They are abundant in luminal epithelial sheetswhere they maintain epithelial cell polarity. Different tissues exhibit different claudincomposition.Product Description: The claudin superfamily consists of many structurally relatedproteins in humans. These proteins, which include claudin 1 through 18, are importantstructural and functional components of tight junctions in paracellular transport.Claudins are located in both epithelial and endothelial cells in all tight junction-bearingtissues. Claudins, which consist of four trans-membrane domains and two extracellularloops, make up tight junction strands.Claudin-5 Rabbit PolyclonalCatalog No. Size FormatE3750 0.1ml ConcentrateE3752 0.5ml ConcentrateE3754 1.0ml ConcentrateE3751 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of mouse claudin-5.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: Cytoplasm, MembraneClaudin-11 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE8770 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE8772 0.5ml Concentrate C-terminus of human claudin-11 protein.E8774 1.0ml Concentrate Application: IHC-PE8771 7.0ml Ready-to-use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Mouse Brain, TestisCellular Localization: MembraneHuman Lung stained withanti-Claudin-5 antibody E375Human Testis stained withanti-Claudin-11 antibody E877Product Description: Claudin 1-18 proteins are a family of transmembrane proteinsassociated with tight junctions. Tight junctions are specialized regions of cell to cellcontact, made up of a network of strands to act as a molecular gasket for preventing theleakage of ions and water between cells. They are abundant in luminal epithelial sheetswhere they maintain epithelial cell polarity. Different tissues exhibit different claudincomposition.Product Description: The claudin superfamily consists of many structurally relatedproteins in humans. These proteins, which include claudin 1 through 18, are importantstructural and functional components of tight junctions in paracellular transport.Claudins are located in both epithelial and endothelial cells in all tight junction-bearingtissues. Claudin-11 plays a major role in tight junction-specific obliteration of theintercellular space.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 39

c-Met** Rabbit MonoclonalCatalog No. Size FormatM3440 0.1ml ConcentrateM3442 0.5ml ConcentrateM3444 1.0ml ConcentrateM3441 7.0ml Ready-to-useClone: SP44Immunogen: Synthetic peptide corresponding toC-terminus of human c-Met protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Membranec-Met (Paired 1003) Rabbit PolyclonalCatalog No. Size FormatE18110 0.1ml ConcentrateE18112 0.5ml ConcentrateE18114 1.0ml ConcentrateE18111 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding to theregion of c-Met that contains non-phosphorylatedtyrosine 1003.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-Met antibody M344Human Breast Carcinoma stained withanti-c-Met (Paired 1003) antibody E1811Product Description: The c-Met oncogene was originally isolated from humanosteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Metproto-oncogene product was identified as a transmembrane receptor-like proteinwith tyrosine kinase activity that is expressed in many tissues. The c-Met gene producthas been identified as the cell surface receptor for hepatocyte growth factor, aplasminogen-like protein thought to be a humoral mediator of liver regeneration.Product Description: The c-Met oncogene was identified as a transmembranereceptor-like protein with tyrosine kinase activity. The c-Met gene product has beenidentified as the cell surface receptor for hepatocyte growth factor and is involved in cellproliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that isnon-phosphorylated at 1003 tyrosine residue.c-Met Rabbit PolyclonalCatalog No. Size FormatE10610 0.1ml ConcentrateE10612 0.5ml ConcentrateE10614 1.0ml ConcentrateE10611 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminusof human c-Met protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Ovarian CarcinomaCellular Localization: Cytoplasm, Membranec-Met (Paired 1349) Rabbit PolyclonalCatalog No. Size FormatE18130 0.1ml ConcentrateE18132 0.5ml ConcentrateE18134 1.0ml ConcentrateE18131 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding to theregion of c-Met that contains non-phosphorylatedtyrosine 1349.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneHuman Ovarian Carcinoma stained withanti-c-Met antibody E1061Human Breast Carcinoma stained withanti-c-Met (Paired 1349) antibody E1813Product Description: The c-Met oncogene was originally isolated from humanosteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Metproto-oncogene product was identified as a transmembrane receptor-like protein withtyrosine kinase activity that is expressed in many tissues. The c-Met gene producthas been identified as the cell surface receptor for hepatocyte growth factor, aplasminogen-like protein thought to be a humoral mediator of liver regeneration.Product Description: The c-Met oncogene was identified as a transmembranereceptor-like protein with tyrosine kinase activity. The c-Met gene product has beenidentified as the cell surface receptor for hepatocyte growth factor and is involved in cellproliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that isnon-phosphorylated at 1003 tyrosine residue.c-Met (Paired 1003) Rabbit MonoclonalCatalog No. Size FormatM3590 0.1ml ConcentrateM3592 0.5ml ConcentrateM3594 1.0ml ConcentrateM3591 7.0ml Ready-to-useClone: SP59Immunogen: Synthetic peptide corresponding to theregion of c-Met that contains non-phosphorylatedtyrosine 1003.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Membranec-Met (Phospho Y1003) Rabbit MonoclonalCatalog No. Size FormatM3580 0.1ml ConcentrateM3582 0.5ml ConcentrateM3584 1.0ml ConcentrateM3581 7.0ml Ready-to-useClone: SP58Immunogen: Synthetic peptide corresponding tothe region of c-Met that contains phosphorylatedtyrosine 1003.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-Met (Paired 1003) antibody M359Human Breast Carcinoma stained withanti-c-Met (Phospho Y1003) antibody M358Product Description: The c-Met oncogene was identified as a transmembranereceptor-like protein with tyrosine kinase activity. The c-Met gene product has beenidentified as the cell surface receptor for hepatocyte growth factor and is involved in cellproliferation, scattering, morphogenesis, and survival. This antibody labels c-Met that isnon-phosphorylated at 1003 tyrosine residue.Product Description: The c-Met oncogene was identified as a transmembranereceptor-like protein with tyrosine kinase activity. It is a cell surface receptor forhepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis,and survival. This antibody is specific for Met (c-Met), only when phosphorylated attyrosine 1003.40Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary Antibodiesc-Met (Phospho Y1003) Rabbit PolyclonalCatalog No. Size FormatE18100 0.1ml ConcentrateE18102 0.5ml ConcentrateE18104 1.0ml ConcentrateE18101 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tothe region of c-Met that contains phosphorylatedtyrosine 1003.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneHuman Breast Carcinoma stained withanti-c-Met (Phospho Y1003) antibody E1810Connexin-43 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2740 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE2742 0.5ml Concentrate a segment of the 3rd cytoplasmic domain ofE2744 1.0ml Concentrate rat connexin-43.E2741 7.0ml Ready-to-use Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: HeartCellular Localization: CytoplasmControl Lysate: MCF7Molecular Weight: 43 kDaHuman Heart stained withanti-Connexin-43 antibody E274Product Description: The c-Met oncogene was identified as a trans-membranereceptor-like protein with tyrosine kinase activity. It is a cell surface receptor forhepatocyte growth factor and involves in cell proliferation, scattering, morphogenesis,and survival. This antibody is specific for Met (c-Met), only when phosphorylated attyrosine 1003.Product Description: The connexins are a group of gap junction proteins which forma hexamer to compose a connexon. Clusters of connexins form a gap junction throughwhich low molecular weight proteins may diffuse from cell to cell. Connexins are alsothought to be essential for the development of labor contractions.c-Met (Phospho Y1234/1235) Rabbit PolyclonalCatalog No. Size FormatE18140 0.1ml ConcentrateE18142 0.5ml ConcentrateE18144 1.0ml ConcentrateE18141 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding to theregion of c-Met that contains phosphorylated tyrosine1234/1235.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneCorticotropin Releasing Factor (CRF) Rabbit PolyclonalCatalog No. Size FormatE10250 0.1ml ConcentrateE10252 0.5ml ConcentrateE10254 1.0ml ConcentrateE10251 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman CRF protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain, PlacentaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-c-Met (Phospho Y1234/1235) antibody E1814Human Brain stained withanti-Corticotropin Releasing Factor (CRF) antibody E1025Product Description: The c-Met oncogene was originally isolated from humanosteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The c-Met proteinwas identified as a trans-membrane receptor-like protein with tyrosine kinase activity.It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation,scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), onlywhen phosphorylated at tyrosine 1234 and 1235.Product Description: Corticotropin Releasing Factor (CRF), a 41 amino acidsneuropeptide, belongs to a family of structurally related peptides including urocortin,sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal glandaxis, and in endocrine, autonomic and behavioral responses to stress. CRF is widelydistributed in the brain and peripheral nervous system with the highest levels in thehypothalamic paraventricular nucleus.c-Met (Phospho Y1349) Rabbit PolyclonalCatalog No. Size FormatE18120 0.1ml ConcentrateE18122 0.5ml ConcentrateE18124 1.0ml ConcentrateE18121 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding tothe region of c-Met that contains phosphorylatedtyrosine 1349.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: MembraneCOX-1 Rabbit PolyclonalCatalog No. Size FormatE14310 0.1ml ConcentrateE14312 0.5ml ConcentrateE14314 1.0ml ConcentrateE14311 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human COX-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Esophageal CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-c-Met (Phospho Y1349) antibody E1812Human Esophageal Carcinoma stained withanti-COX-1 antibody E1431Product Description: The c-Met oncogene was originally isolated from humanosteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The c-Met proteinwas identified as a trans-membrane receptor-like protein with tyrosine kinase activity.It is a cell surface receptor for hepatocyte growth factor and involves in cell proliferation,scattering, morphogenesis, and survival. This antibody is specific for Met (c-Met), onlywhen phosphorylated at tyrosine 1349.Product Description: Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved inthe conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar instructure and function, though they vary in expression. COX-1 is normally expressed inmost cell types, whereas COX-2 expression is at low levels unless induced by hormonalstimuli. In some breast cancer and bladder carcinoma studies, COX-1 was expressed inthe stroma cells adjacent to the tumors but not in tumor cells.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 41

COX-2 Rabbit MonoclonalCripto-1 Rabbit PolyclonalCatalog No. Size FormatClone: SP21Catalog No. Size FormatClone: N/AM3210 0.1ml ConcentrateM3212 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of rat COX-2.E18430 0.1ml ConcentrateE18432 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromhuman cripto-1.M3214 1.0ml ConcentrateApplication: IHC-PE18434 1.0ml ConcentrateApplication: IHC-PM3211 7.0ml Ready-to-useSpecies Reactivity: Human*E18431 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast Carcinoma, Colon, LungControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, MembraneCellular Localization: MembraneHuman Breast Carcinoma stained withanti-COX-2 antibody M321Human Colon Carcinoma stained withanti-Cripto-1 antibody E1843Product Description: COX2 (Cyclooxygenase-2 COX2) is an inducible enzyme inresponse to growth factors, tumor promoters, and cytokines. COX2 is induced by awide variety of stimuli and was initially identified as immediate-early growth responsegene. In addition, COX2 expression markedly increased in 85-90% of human colorectaladenocarcinoma whereas COX1 levels remain unchanged.Product Description: The cripto gene is expressed both in embryonic stem cells andduring the early phases of embryo development, while in adults it is reactivated in awide range of epithelial cancers. The expression of Cripto-1 may represent a usefulmarker to identify cancer stem cells in melanoma, and possibly other aggressive tumorsas well. Caveolin-1 can be down-regulated during the process of Cripto-1 mediatedtumorigenesis in the mammary gland.COX-2 Rabbit Polyclonalc-TEN Rabbit MonoclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: SP83E3030 0.1ml ConcentrateE3032 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of rat COX-2.M3830 0.1ml ConcentrateM3832 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human c-TEN protein.E3034 1.0ml ConcentrateApplication: IHC-PM3834 1.0ml ConcentrateApplication: IHC-PE3031 7.0ml Ready-to-useSpecies Reactivity: Human*M3831 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast Carcinoma, Colon, LungControl Tissue: Liver CarcinomaCellular Localization: Cytoplasm, MembraneCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-COX-2 antibody E303Human Liver Carcinoma stained withanti-c-TEN antibody M383Product Description: COX2 (Cyclooxygenase-2 COX2) is an inducible enzyme inresponse to growth factors, tumor promoters, and cytokines. COX2 is induced by awide variety of stimuli and was initially identified as immediate-early growth responsegene. In addition, COX2 expression markedly increased in 85-90% of human colorectaladenocarcinoma whereas COX1 levels remain unchanged.Product Description: c-TEN/TNS4 links the focal adhesion and cell adhesion signaling tothe actin cytoskeleton. It may be involved in cell survival and migration. The protein maybe involved in cell migration, cartilage development and in linking signal transductionpathways to the cytoskeleton and promote apoptosis, via its cleavage by caspase-3.CREB-Binding Protein (CBP ) Rabbit PolyclonalCatalog No. Size FormatE13840 0.1ml ConcentrateE13842 0.5ml ConcentrateE13844 1.0ml ConcentrateE13841 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding to aa2422-2441 of human CBP.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Nucleusc-TEN Rabbit PolyclonalCatalog No. Size FormatE18190 0.1ml ConcentrateE18192 0.5ml ConcentrateE18194 1.0ml ConcentrateE18191 7.0ml Ready-to-useClone: SP58Immunogen: Synthetic peptide corresponding toC-terminus of human c-TEN protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-CREB-Binding Protein (CBP ) antibody E1384Human Colon Carcinoma stained withanti-c-TEN antibody E1819Product Description: CBP (CREB-binding protein) is a 265kDa nuclear protein whichprobably has many of the properties expected of a CREB co-activator. It showssubstantial sequence homology with p300.Product Description: c-TEN/TNS4 links the focal adhesion and cell adhesion signaling tothe actin cytoskeleton. It may be involved in cell survival and migration. The protein maybe involved in cell migration, cartilage development and in linking signal transductionpathways to the cytoskeleton and promote apoptosis, via its cleavage by caspase-3.42Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesCullin-1 (CUL-1) Rabbit PolyclonalCatalog No. Size FormatE3790 0.1ml ConcentrateE3792 0.5ml ConcentrateE3794 1.0ml ConcentrateE3791 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cullin-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon Carcinoma, TonsilCellular Localization: NucleusCyclin B1 Rabbit PolyclonalCatalog No. Size FormatE4100 0.1ml ConcentrateE4102 0.5ml ConcentrateE4104 1.0ml ConcentrateE4101 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cyclin B1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusHuman Colon Carcinoma stained withanti-Cullin-1 (CUL-1) antibody E379Human Breast Carcinoma stained withanti-Cyclin B1 antibody E410Product Description: Cullin-1 (CUL-1) is a member of the family of human cullin genes(cullin-1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc53 gene. Cullin-1 forms acomplex with human p19skp1 and F box protein p45skp2, both of which, together withcdc53, as three subunits, form an ubiquitin ligase controlling proteolysis of G1 cell cycleregulatory proteins. The cullin-1/p19skp1/p45skp2 complex is thought to play a role inthe ubiquitination of G1 regulatory proteins.Product Description: Cyclin B1 is a regulatory protein involved in mitosis. Cyclin B1associates with inactive p34 (cdc2) to facilitate phosphorylation of p34 (cdc2) at aa14Thr and 15Tyr. Cyclin B1 is expressed predominantly during G2/M phase of the cellcycle. The cyclin B1-p34 (cdc2) complex plays a critical role in G2 to M transition. CyclinB1 also contributes to the switch-like all or none behavior of the cell in deciding tocommit to mitosis.Cullin-2 (CUL-2) Rabbit PolyclonalCatalog No. Size FormatE4160 0.1ml ConcentrateE4162 0.5ml ConcentrateE4164 1.0ml ConcentrateE4161 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cullin-2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Tonsil stained withanti-Cullin-2 (CUL-2) antibody E416Cyclin D1 Rabbit MonoclonalCatalog No. Size FormatM3040 0.1ml ConcentrateM3042 0.5ml ConcentrateM3044 1.0ml ConcentrateM3041 7.0ml Ready-to-useClone: SP4Immunogen: Synthetic peptide fromC-terminus of human cyclin D1.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Mantle Cell Lymphoma,TonsilCellular Localization: NucleusControl Lysate: MCF7Molecular Weight: 36 kDaHuman Mantle Cell Lymphoma stained withanti-Cyclin D1 antibody M304Product Description: Cullin-2 (CUL-2) is a member of human cullin gene family(CUL-1, -2, -3, -4a, -4b, and -5). It is the core component of multiple cullin-RING-basedECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes.The ECS ligase complexes mediate the ubiquitination of target proteins such ashypoxia-inducible factor (HIF).Product Description: Cyclin D1 or PRAD-1 or BCL-1 is one of the key cell cycle regulators,and functions in association with CDK4 and / or CDK6 by phosphorylating the Rbprotein. It is a putative proto-oncogene overexpressed in a wide variety of humanneoplasms including mantle cell lymphomas (MCL).CXCR4 Rabbit PolyclonalCyclin D1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18440 0.1ml ConcentrateE18442 0.5ml ConcentrateImmunogen: Synthetic peptide derived from thehuman CXCR4.E1540 0.1ml ConcentrateE1542 0.5ml ConcentrateImmunogen: Synthetic peptide fromC-terminus of human cyclin D1.E18444 1.0ml ConcentrateApplication: IHC-PE1544 1.0ml ConcentrateApplication: IHC-P, WBE18441 7.0ml Ready-to-useSpecies Reactivity: Human*E1541 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: MembraneControl Tissue: Mantle Cell Lymphoma,TonsilCellular Localization: NucleusControl Lysate: MCF7Molecular Weight: 36 kDaHuman Placenta stained withanti-CXCR4 antibody E1844Human Mantle Cell Lymphoma stained withanti-Cyclin D1 antibody E154Product Description: CXCR4 is a principal coreceptor for T cell tropic strains of HIV1fusion and entry of human white blood cells. CXCR4 is also required for the infectionby dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. Thechemokine SDF1 is the ligand for CXCR4 and prevents infection by T tropic HIV1. CXCR4is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells,and umbilical cord endothelial cells.Product Description: Cyclin D1 or PRAD-1 or BCL-1 is one of the key cell cycle regulators,and functions in association with CDK4 and / or CDK6 by phosphorylating the Rbprotein. It is a putative proto-oncogene overexpressed in a wide variety of humanneoplasms including mantle cell lymphomas (MCL).US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 43

Cyclin E Rabbit PolyclonalD4-GDI Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE3600 0.1ml ConcentrateE3602 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman cyclin E.E2430 0.1ml ConcentrateE2432 0.5ml ConcentrateImmunogen: Recombinant full-lengthhuman D4-GDI protein.E3604 1.0ml ConcentrateApplication: IHC-PE2434 1.0ml ConcentrateApplication: IHC-P, WBE3601 7.0ml Ready-to-useSpecies Reactivity: Human*E2431 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaControl Tissue: Placenta, TonsilCellular Localization: NucleusCellular Localization: Cytoplasm, NucleusControl Lysate: JurkatMolecular Weight: 23 kDaHuman Breast Carcinoma stained withanti-Cyclin E antibody E360Human Placenta stained withanti-D4-GDI antibody E243Product Description: Cyclin E is a regulatory subunit of CDK-2 and controls G1/Stransition during the mammalian cell cycle. Multiple isoforms of Cyclin E are expressedonly in tumors but not in the normal tissues, suggesting a post-transcriptional regulationof Cyclin E. In vitro analysis indicated that these truncated variant isoforms of Cyclin E areable to phosphorylate histone H1.Product Description: D4-GDI belongs to the family of Rho-GDIs or GDP-dissociationinhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI-1, D4-GDI/RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins, essentialfor GTP-loading and activation of GTPase activity. D4- GDI is located in the cytoplasmand is involved in the regulation of the membrane association and dissociation cycle ofRho/Rac proteins.CYR61 Rabbit PolyclonalD5DR Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18300 0.1ml ConcentrateE18302 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding tointernal region of human CYR61.E12580 0.1ml ConcentrateE12582 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding to aa459-477 of human D5DR.E18304 1.0ml ConcentrateApplication: IHC-PE12584 1.0ml ConcentrateApplication: IHC-PE18301 7.0ml Ready-to-useSpecies Reactivity: Human*E12581 7.0ml Ready-to-useSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Colon, PlacentaControl Tissue: BrainCellular Localization: CytoplasmCellular Localization: Cytoplasm, MembraneHuman Colon stained withanti-CYR61 antibody E1830Human Brain stained withanti-D5DR antibody E1258Product Description: CYR61 is a secreted cysteine-rich heparin binding protein,encoded by a growth factor inducible immediate-early gene. CYR61 is a member ofa distinct family of angiogenic and vasculogenic regulators designated CCN proteins.As an angiogenic inducer, CYR61 binds to the cell surface receptor to promotes DNAsynthesis of human vascular endothelial cells. CYR61 is also detected in a wide varietyof tumors as it induces tumor growth.Product Description: The dopamine receptors are divided into two classes, D1 and D2,which differ in their functional characteristics. D1DR and D5DR compose the D1 class ofdopamine receptors, and D2DR, D3DR and D4DR compose the D2 class. D1 receptorsstimulate adenylyl cyclase, while D2 receptors inhibit adenylyl cyclase activity.Cytochrome C Rabbit PolyclonalCatalog No. Size FormatE12410 0.1ml ConcentrateE12412 0.5ml ConcentrateE12414 1.0ml ConcentrateE12411 7.0ml Ready-to-useClone: N/AImmunogen: Recombinant full length humancytochrome C protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmDAPK2 Rabbit PolyclonalCatalog No. Size FormatE16860 0.1ml ConcentrateE16862 0.5ml ConcentrateE16864 1.0ml ConcentrateE16861 7.0ml Ready-to-useClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human DAPK2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Brain stained withanti-Cytochrome C antibody E1241Human Breast Carcinoma stained withanti-DAPK2 antibody E1686Product Description: Cytochrome C is an electron transporting protein that resideswithin the inter-membrane space of the mitochondria, where it plays a critical role inthe process of oxidative phosphorylation and production of cellular ATP. Cytocrome Cis up-regulated and it is rapidly released from the mitochondria into the cytosol inapoptotic processes. Cytosolic cytochrome C participates in the activation of apoptoticeffectors such as caspase-3 and ICE family molecule.Product Description: Death-associated protein kinase 2 (DAPK2) is a protein belongingto the serine/threonine protein kinase family. It is pro-apoptotic calcium-regulatedserine/threonine kinase containing death domain. Ectopic expression of DAPKinduces cell death and suppresses oncogenic transformation. DAPK mediates IFNgamma induced apoptosis. The messenger RNA of DAPK2 is expressed in multiplehuman tissues.44Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesDesmin Rabbit PolyclonalCatalog No. Size FormatE2570 0.1ml ConcentrateE2572 0.5ml ConcentrateE2574 1.0ml ConcentrateE2571 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide mapping near theC-terminus of human desmin.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Sarcoma, Skeletal MuscleCellular Localization: CytoplasmDJ-1 Rabbit PolyclonalCatalog No. Size FormatE8760 0.1ml ConcentrateE8762 0.5ml ConcentrateE8764 1.0ml ConcentrateE8761 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom C-terminus of human DJ-1 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BreastCellular Localization: Cytoplasm, NucleusControl Lysate: JurkatMolecular Weight: 22 kDaHuman Skeletal Muscle stained withanti-Desmin antibody E257Human Breast stained withanti-DJ-1 antibody E876Product Description: Desmin is an intermediate filament protein of both smooth andstriated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as wellas smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to theZ-bands giving a characteristic striated appearance.Product Description: DJ-1 protein has 189 amino acids and was cloned from a HeLacell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. Theexpression of DJ-1 is induced by growth signals and is translocated from the cytoplasmto the nucleus during the S phase, although no nuclear localization signals are present.It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved inRas-related signal transduction pathway.DFF-45 Rabbit PolyclonalCatalog No. Size FormatE3900 0.1ml ConcentrateE3902 0.5ml ConcentrateE3904 1.0ml ConcentrateE3901 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human DFF-45 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmDOG-1 Rabbit MonoclonalCatalog No. Size FormatM3310 0.1ml ConcentrateM3312 0.5ml ConcentrateM3314 1.0ml ConcentrateM3311 7.0ml Ready-to-UseClone: SP31Immunogen: Synthetic peptide of human DOG-1protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Gastrointestinal Stromal TumorCellular Localization: Cytoplasm, MembraneHuman Colon Carcinoma stained withanti-DFF-45 antibody E390Human Gastrointestinal Stromal Tumor stained withanti-DOG-1 antibody M331Product Description: DFF45 and DFF40 (ICAD and CAD) are two subunits ofheterodimeric caspase activated DNAse. DFF45/ ICAD (Inhibitor of Caspase ActivatedDNAse) acts as a chaperon for appropriate folding of DFF40 and it also acts as inhibitorof DFF40. The activation of caspase 3 cleaves DFF to release DFF40/CAD, whicholigomerizes into a large active complex and eventually causes the degradation of DNAin nuclei. Hence cleavage of DFF45 is an important step in apoptosis.Product Description: DOG-1, a novel marker, is expressed ubiquitously ingastrointestinal stromal tumors (GIST) irrespective of KIT or PDGFR alphamutation status.DHFR Rabbit PolyclonalCatalog No. Size FormatE18220 0.1ml ConcentrateE18222 0.5ml ConcentrateE18224 1.0ml ConcentrateE18221 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human DHFR.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusDOG-1 Rabbit PolyclonalCatalog No. Size FormatE16710 0.1ml ConcentrateE16712 0.5ml ConcentrateE16714 1.0ml ConcentrateE16711 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide of human DOG-1protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Gastrointestinal Stromal TumorCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-DHFR antibody E1822Human Gastrointestinal Stromal Tumor stained withanti-DOG-1 antibody E1671Product Description: Dihydrofolate reductase (DHFR) converts dihydrofolate intotetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certainamino acids. While the functional dihydrofolate reductase gene has been mapped tochromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductaselike genes have been identified on separate chromosomes. Dihydrofolate reductasedeficiency has been linked to megaloblastic anemia.Product Description: DOG-1, a novel marker, is expressed ubiquitously ingastrointestinal stromal tumors (GIST) irrespective of KIT or PDGFR alphamutation status.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 45

Primary AntibodiesE2F-2 Rabbit PolyclonalCatalog No. Size FormatE13610 0.1ml ConcentrateE13612 0.5ml ConcentrateE13614 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Synthetic peptidecorresponding to N-terminus of humanE2F-2.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: HepG2Molecular Weight: 48 kDaE1361Epidermal Growth Factor Receptor (EGFR) Rabbit MonoclonalCatalog No. Size FormatM3840 0.1ml ConcentrateM3842 0.5ml ConcentrateM3844 1.0ml ConcentrateM3841 7.0ml Ready-to-UseClone: SP84Immunogen: Synthetic peptide correspondingto C-terminus of human EGFR protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung Squamous CellCarcinomaCellular Localization: MembraneControl Lysate: A431Molecular Weight: 175 kDaHuman Lung Squamous Cell Carcinoma stained withanti-Epidermal Growth Factor Receptor(EGFR) antibody M384Product Description: E2F transcription factors are functionally regulated by bindingto Rb p110, p107, and p130. E2F-2 is regulated by complex formation with Rb p110.E2F family members bind DNA as heterodimers with members of the DP family ofpolypeptides.Product Description: EGFR is a type I receptor tyrosine kinase with sequencehomology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor(EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors ofbreast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, andendometrium. It is predominantly present in squamous cell carcinomas.EGFL7 Rabbit PolyclonalCatalog No. Size FormatE19130 0.1ml ConcentrateE19132 0.5ml ConcentrateE19134 1.0ml ConcentrateE19131 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aninternal region of human EGFL7 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: Cytoplasm, MembraneEpidermal Growth Factor Receptor (EGFR) Rabbit MonoclonalCatalog No. Size FormatM3090 0.1ml ConcentrateM3092 0.5ml ConcentrateM3094 1.0ml ConcentrateM3091 7.0ml Ready-to-UseClone: SP9Immunogen: Synthetic peptide from C-terminus ofhuman EGFR protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung, PlacentaCellular Localization: Cytoplasm, MembraneHuman Placenta stained withanti-EGFL7 antibody E1913Human Placenta stained withanti-Epidermal Growth Factor Receptor (EGFR) antibody M309Product Description: Epidermal growth factor-like protein 7 (EGFL7), also known as VEstatin,MEGF7, Notch4-like protein or Zneu1, is a 29.6 kDa protein produced and secretedby endothelium. The protein is also expressed by some normal and malignant tissues.The main function of EGFL7 is to regulate angiogenesis and vasculogenesis. A high levelof EFGL-7 can be found in tissues with active angiogenesis of a variety of disorders.Product Description: EGFR is a type I receptor tyrosine kinase with sequencehomology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor(EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors ofbreast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, andendometrium. It is predominantly present in squamous cell carcinomas.Ephrin A4 Rabbit PolyclonalCatalog No. Size FormatE11150 0.1ml ConcentrateE11152 0.5ml ConcentrateE11154 1.0ml ConcentrateE11151 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant full length human ephrinA4 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: Cytoplasm, MembraneEpidermal Growth Factor Receptor (EGFR) Mouse MonoclonalCatalog No. Size FormatE6690 0.1ml ConcentrateE6692 0.5ml ConcentrateE6694 1.0ml ConcentrateE6691 7.0ml Ready-to-UseClone: SPM341Immunogen: Extracellular domain of humanrecombinant EGFR protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Placenta, Skin, Squamous CellCarcinomaCellular Localization: MembraneHuman Brain stained withanti-Ephrin A4 antibody E1115Human Placenta stained withanti-Epidermal Growth Factor Receptor (EGFR) antibody E669Product Description: Ephrin-A4, also known as LERK-4 or ELF-4, is a ligand for theerythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases andit binds EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Ephrin-A4 interactswith activated B lymphocytes and dendritic cells and it is expressed in the adult spleen,lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liverand kidney.Product Description: EGFR is a type I receptor tyrosine kinase with sequencehomology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor(EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors ofbreast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, andendometrium. It is predominantly present in squamous cell carcinomas.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 47

Epidermal Growth Factor Receptor (EGFR) Rabbit PolyclonalCatalog No. Size FormatE18170 0.1ml ConcentrateE18172 0.5ml ConcentrateE18174 1.0ml ConcentrateE18171 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide correspondingto C-terminus of human EGFR protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Esophageal Carcinoma,PlacentaCellular Localization: MembraneControl Lysate: A431Molecular Weight: 170 kDaHuman Esophageal Carcinoma stained with anti-Epidermal Growth Factor Receptor (EGFR) antibody E1817ERCC1 Rabbit PolyclonalCatalog No. Size FormatE18890 0.1ml ConcentrateE18892 0.5ml ConcentrateE18894 1.0ml ConcentrateE18891 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant human ERCC1protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung Squamous CellCarcinoma, Rectal CarcinomaCellular Localization: NucleusControl Lysate: HeLaMolecular Weight: 39 kDaHuman Lung Squamous Cell Carcinoma stained withanti-ERCC1 antibody E1889Product Description: EGFR is a type I receptor tyrosine kinase with sequencehomology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to Epidermal Growth Factor(EGF), Transforming Growth Factor-alpha (TGF-alpha), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors ofbreast, brain, bladder, lung, gastric, head, neck, esophagus, cervix, vulva, ovary, andendometrium. It is predominantly present in squamous cell carcinomas.Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing)polypeptide is required for nucleotide excision repair (NER) of damaged DNAand is homologous to S. cerevisiae RAD10, which functions in repair and mitoticintrachromosomal recombination. The NER mechanism involves dual incisions on bothsides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ ofthe DNA lesion while the ERCC1-XPF complex makes the 5’ incision.ERAB Rabbit PolyclonalERCC1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE10320 0.1ml ConcentrateE10322 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human ERAB protein.E18550 0.1ml ConcentrateE18552 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human ERCC1 protein.E10324 1.0ml ConcentrateApplication: IHC-PE18554 1.0ml ConcentrateApplication: IHC-PE10321 7.0ml Ready-to-UseSpecies Reactivity: Human*E18551 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Alzheimer’s Brain, TonsilControl Tissue: ColonCellular Localization: CytoplasmCellular Localization: NucleusHuman Alzheimer’s Brain stained withanti-ERAB antibody E1032Human Colon stained withanti-ERCC1 antibody E1855Product Description: ERAB also known as 3-hydroxyacyl-CoA dehydrogenase type-2belongs to the short-chain dehydrogenases/reductases (SDR) family. It is one of themutated genes associated with early onset Alzheimer’s disease. It is shown to beexpressed at high levels in Alzheimer’s disease-affected brain and may be related toneuronal dysfunction in Alzheimer’s disease.Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing)polypeptide is required for nucleotide excision repair (NER) of damaged DNAand is homologous to S. cerevisiae RAD10, which functions in repair and mitoticintrachromosomal recombination. The NER mechanism involves dual incisions on bothsides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ ofthe DNA lesion while the ERCC1-XPF complex makes the 5’ incision.ERCC1 Rabbit MonoclonalERK-1 Rabbit PolyclonalCatalog No. Size FormatClone: SP68Catalog No. Size FormatClone: N/AM3680 0.1ml ConcentrateM3682 0.5ml ConcentrateM3684 1.0ml ConcentrateM3681 7.0ml Ready-to-UseImmunogen: Recombinant human ERCC1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Non-Small Cell Lung CarcinomaCellular Localization: NucleusE3560 0.1ml ConcentrateE3562 0.5ml ConcentrateE3564 1.0ml ConcentrateE3561 7.0ml Ready-to-UseImmunogen: Synthetic peptide derived fromC-terminus of human ERK-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Lung Carcinoma stained withanti-ERCC1 antibody M368Human Breast Carcinoma stained withanti-ERK-1 antibody E356Product Description: The mammalian ERCC1 (Excision Repair Cross Complementing)polypeptide is required for nucleotide excision repair (NER) of damaged DNAand is homologous to S. cerevisiae RAD10, which functions in repair and mitoticintrachromosomal recombination. The NER mechanism involves dual incisions on bothsides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3’ ofthe DNA lesion while the ERCC1-XPF complex makes the 5’ incision.Product Description: ERK-1/extracellular signal-regulated kinase 1/p44 MAP kinase/microtubule-associated protein 2 kinase/myelin basic protein kinase/EGF receptorT669 kinase is ubiquitously expressed in various tissues and cells. It gets activated byphosphorylation of neighboring serine and threonine residues by MEK-1 and MEK-2 inresponse to diverse stimuli like growth factors and mitogens.48Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesERK-2 Rabbit PolyclonalCatalog No. Size FormatE3970 0.1ml ConcentrateE3972 0.5ml ConcentrateE3974 1.0ml ConcentrateE3971 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of mouse ERK-2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusEstrogen Receptor (ER) Rabbit PolyclonalCatalog No. Size FormatE1640 0.1ml ConcentrateE1642 0.5ml ConcentrateE1644 1.0ml ConcentrateE1641 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human estrogen receptor.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusHuman Breast Carcinoma stained withanti-ERK-2 antibody E397Human Breast Carcinoma stained withanti-Estrogen Receptor (ER) antibody E164Product Description: Extracellular signal-regulated kinase 2 (ERK-2) is a serine-threoninekinase phosphorylates microtubule associated protein-2 and myelin basic protein. Thiskinase is an important proximal component of the MAP kinase pathway involved intransmitting the signals from growth factors, neurotransmitters and hormones at the cellsurface to the transcriptional events in the nucleus.Product Description: This antibody recognizes a protein of 67kDa, which is identifiedas estrogen receptor (ER). This antibody strongly stains the nucleus of epithelial cells inbreast carcinomas. The ER is an important regulator of growth and differentiation in themammary gland.ESA/Ep-CAM Mouse MonoclonalCatalog No. Size Format Clone: SPM491E11170 0.1ml Concentrate Immunogen: Recombinant human ESA proteinE11172 0.5ml Concentrate expressed in E. coli.E11174 1.0ml Concentrate Application: IHC-PE11171 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Breast CarcinomaCellular Localization: MembraneEZH2 Rabbit PolyclonalCatalog No. Size FormatE19090 0.1ml ConcentrateE19092 0.5ml ConcentrateE19094 1.0ml ConcentrateE19091 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human EZH2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: NucleusHuman Breast Carcinoma stained withanti-ESA/Ep-CAM antibody E1117Human Prostate Carcinoma stained withanti-EZH2 antibody E1909Product Description: EGP40 is a 40kDa transmembrane epithelial glycoprotein, alsoidentified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule(Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vastmajority of carcinomas.Product Description: KMT6 / Enhancer of zeste homologue 2 (EZH2) is a histonelysine methyl transferase which is associated with transcriptional repression. EZH2 isubiquitously expressed during early embryo genesis, and becomes restricted to thecentral and peripheral nervous systems and sites of fetal hematopoiesis during laterdevelopment. The expression of EZH2 is highly expressed in follicular T cells and avariety of tumors including lymphoma, breast and prostate cancer.Estrogen Receptor (ER) Rabbit MonoclonalCatalog No. Size FormatM3010 0.1ml ConcentrateM3012 0.5ml ConcentrateM3014 1.0ml ConcentrateM3011 7.0ml Ready-to-UseClone: SP1Immunogen: Synthetic peptide derivedfrom C-terminus of human estrogenreceptor alpha.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusControl Lysate: MCF7Molecular Weight: 67 kDaHuman Breast Carcinoma stained with anti-EstrogenReceptor (ER) antibody M301F4/80 Rabbit MonoclonalCatalog No. Size FormatM4150 0.1ml ConcentrateM4152 0.5ml ConcentrateM4154 1.0ml ConcentrateM4151 7.0ml Ready-to-UseClone: SP115Immunogen: Synthetic peptide derived fromN-terminus of mouse F4/80 protein.Application: IHC-PSpecies Reactivity: Mouse*Isotype: Rabbit IgGControl Tissue: Mouse LiverCellular Localization: MembraneMouse Liver stained withanti-F4/80 antibody M415Product Description: This antibody recognizes a protein of 67kDa, which is identifiedas estrogen receptor (ER). This antibody strongly stains the nucleus of epithelial cells inbreast carcinomas. The ER is an important regulator of growth and differentiation in themammary gland.Product Description: F4/80 is a cell surface glycoprotein and is reported to vary duringmacrophage maturation and activation. The F4/80 antigen is expressed on a widerange of mature tissue macrophages including Kupffer cells, Langerhans, microglia,macrophages and a subpopulation of dendritic cells. The ligands and biologicalfunctions of the F4/80 antigen have not yet been determined but recent studies suggesta role for F4/80 in the generation of efferent CD8+ regulatory T cells.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 49

F4/80 Rabbit PolyclonalFcRH5 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18970 0.1ml ConcentrateE18972 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromN-terminus of mouse F4/80 protein.E19250 0.1ml ConcentrateE19252 0.5ml ConcentrateImmunogen: Synthetic peptide near the C-terminus ofhuman FcRH5 protein.E18974 1.0ml ConcentrateApplication: IHC-PE19254 1.0ml ConcentrateApplication: IHC-PE18971 7.0ml Ready-to-UseSpecies Reactivity: Human*E19251 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: LungControl Tissue: TonsilCellular Localization: MembraneCellular Localization: MembraneHuman Lung stained withanti-F4/80 antibody E1897Human Tonsil stained withanti-FcRH5 antibody E1925Product Description: F4/80 antigen is a 160kDa cell surface glycoprotein expressed inmature tissue macrophages such as Kupffer cells, Langerhans, microglia, macrophageslocated in the gut lamina propria, peritoneal cavity, lung, thymus, and bone marrowstroma. F4/80 is also expressed in a subpopulation of dendritic cells but absent frommacrophages located in T cell areas of the spleen and lymph node. Recent studiessuggest a role for F4/80 in the generation of CD8+ regulatory T cells.Product Description: Fc receptor homologs (FcRH5), also known as IRTA2 proteins, aretransmembrane proteins with sequence homology to classical Fc receptor families.FcRH5 function as activating or inhibitory receptors on discrete B cell populations andare involved in B cell development and differentiation in peripheral lymphoid organs.FcRH5 expression is deregulated in multiple myeloma and Burkitt lymphoma cell lineswith 1q21 abnormalities.FAS Ligand Rabbit PolyclonalCatalog No. Size FormatE2710 0.1ml ConcentrateE2712 0.5ml ConcentrateE2714 1.0ml ConcentrateE2711 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from nearN-terminus of human FAS ligand.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, Testis, TonsilCellular Localization: Cytoplasm, MembraneFGFR-1 Rabbit PolyclonalCatalog No. Size FormatE10580 0.1ml ConcentrateE10582 0.5ml ConcentrateE10584 1.0ml ConcentrateE10581 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human FGFR-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PituitaryCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-FAS Ligand antibody E271Human Breast Carcinoma stained withanti-FGFR-1 antibody E1058Product Description: Fas ligand is a cell surface molecule belonging to the tumornecrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cellsexpress Fas, where Fas ligand is expressed predominantly on activated T cells. Fas andFas ligand are involved in the down-regulation of immune reactions as well as T cellmediatedcytotoxicity.Product Description: Fibroblast growth factors (FGFs) are members of a large family ofstructurally related polypeptides that regulates growth and differentiation in most cells.FGFR-1 is one of the four genes encoding for high affinity cell surface FGF receptors(FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors. Atthe mRNA level, FGFR-1 is highly expressed in developing human tissues such as brain,vascular basement membrane, and skin.FAS-Associated Phosphatase (FAP-1) Rabbit PolyclonalCatalog No. Size FormatE12360 0.1ml ConcentrateE12362 0.5ml ConcentrateE12364 1.0ml ConcentrateE12361 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 2290-2491 of human FAP-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon Carcinoma, PlacentaCellular Localization: CytoplasmFGFR-2 Rabbit PolyclonalCatalog No. Size FormatE10570 0.1ml ConcentrateE10572 0.5ml ConcentrateE10574 1.0ml ConcentrateE10571 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from aa 362-374 ofhuman FGFR-2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Liver CarcinomaCellular Localization: Cytoplasm, MembraneHuman Colon Carcinoma stained withanti-FAS-Associated Phosphatase (FAP-1) antibody E1236Human Breast Carcinoma stained withanti-FGFR-2 antibody E1057Product Description: FAS-associated phosphatase (FAP-1), a novel protein tyrosinephosphatase, regulates FAS-induced apoptosis and nerve growth factor receptor(NGFR)-mediated pro-apoptotic signaling. It has been shown to associate with thefifteen amino acids at the carboxy terminus of FAS. FAP-1 contains three isoforms and amembrane-binding domain. It is present in most tissues with the exception of the liverand skeletal muscle, and is most abundant in lung, kidney and fetal brain.Product Description: Fibroblast growth factors (FGFs) are members of a large family ofstructurally related polypeptides that regulates growth and differentiation in most cells.FGFR-2 is one of the four genes encoding for high affinity cell surface FGF receptors(FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors.FGFR-2 is highly expressed in developing human tissues such as brain, choroids plexus,and lung.50Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesFGFR-3 Rabbit PolyclonalCatalog No. Size FormatE10230 0.1ml ConcentrateE10232 0.5ml ConcentrateE10234 1.0ml ConcentrateE10231 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human FGFR-3 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, Breast CarcinomaCellular Localization: CytoplasmFLK-1/KDR/VEGFR-2 Rabbit PolyclonalCatalog No. Size FormatE3710 0.1ml ConcentrateE3712 0.5ml ConcentrateE3714 1.0ml ConcentrateE3711 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human FLK-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Angiosarcoma, PlacentaCellular Localization: Cytoplasm, MembraneHuman Breast stained withanti-FGFR-3 antibody E1023Human Placenta stained withanti-FLK-1/KDR/VEGFR-2 antibody E371Product Description: Fibroblast growth factors (FGFs) are members of a large family ofstructurally related polypeptides that regulates growth and differentiation in most cells.FGFR-3 is one of the four genes encoding for high affinity cell surface FGF receptors(FGFRs). FGFRs are members of the tyrosine kinase family of growth factor receptors.FGFR-3 is widely expressed in many fetal and adult human and animal tissues.Product Description: FLK-1/KDR/VEGFR2, a cell membrane receptor kinase, is a highaffinity receptor for vascular endothelial growth factor and is putatively involved in thegrowth of endothelial cells and angiogenesis. It contains seven immunoglobulin-likesequences in the extracellular domain and exhibits sequence similarity to FLT-1and FLT-4.FLI-1 Rabbit PolyclonalCatalog No. Size FormatE4280 0.1ml ConcentrateE4282 0.5ml ConcentrateE4284 1.0ml ConcentrateE4281 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human FLI-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Angiosarcoma, Ewing SarcomaCellular Localization: NucleusFLT-1/VEGFR1 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2800 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE2802 0.5ml Concentrate C-terminus of human FLT-1.E2804 1.0ml Concentrate Application: IHC-PE2801 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Angiosarcoma, PlacentaCellular Localization: Cytoplasm, MembraneHuman Ewing Sarcoma stained withanti-FLI-1 antibody E428Human Placenta stained withanti-FLT-1/VEGFR1 antibody E280Product Description: Fli-1 is a member of the ETS-1gene family which includes ETS-1,ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU-1 and PEA-3. Fli-1 is a nuclear protein and itsdefects are the cause of Ewing sarcoma (ES), a highly malignant, metastatic, primitivesmall round cell tumor of bone and soft tissue in children and adolescents.Product Description: FLT-1/vascular endothelial growth factor receptor 1 (VEGFR1), acell membrane receptor kinase, is a high affinity receptor for vascular endothelial growthfactor and is putatively involved in the growth of endothelial cells and angiogenesis. Itcontains seven immunoglobulin like sequences in the extracellular domain and exhibitssequence similarity to FLK-1 and FLT-4.FLK-1 (mouse) Rabbit MonoclonalCatalog No. Size FormatM4230 0.1ml ConcentrateM4232 0.5ml ConcentrateM4234 1.0ml ConcentrateM4231 7.0ml Ready-to-UseClone: SP123Immunogen: Synthetic peptide near the N-terminus ofmouse FLK-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: MembraneFLT-4/VEGFR3 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3870 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE3872 0.5ml Concentrate C-terminus of human FLT-4 protein.E3874 1.0ml Concentrate Application: IHC-PE3871 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: Cytoplasm, MembraneHuman Placenta stained withanti-FLK-1 (mouse) antibody M423Human Placenta stained withanti-FLT-4/VEGFR3 antibody E387Product Description: FLK-1/KDR/VEGFR2, a cell membrane receptor kinase, is a highaffinity receptor for vascular endothelial growth factor and is putatively involved in thegrowth of endothelial cells and angiogenesis. It contains seven immunoglobulin-likesequences in the extracellular domain and exhibits sequence similarity to FLT-1 andFLT-4.Product Description: Three cell membrane receptor tyrosine kinases, FLT (alsodesignated VEGF-R1), FLK-1 (also designated VEGF-R2) and FLT-4 (also designatedVEGF-R3), are putatively involved in the growth of endothelial cells as characterized bythe presence of seven immuno-globulin-like sequences in their extracellular domain. Onthe basis of structural similarity to FLT and FLK-1, it has been speculated that FLT-4 mightrepresent a third receptor for either VEGF or a VEGF-related ligand.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 51

Fn14 Rabbit PolyclonalCatalog No. Size FormatE19080 0.1ml ConcentrateE19082 0.5ml ConcentrateE19084 1.0ml ConcentrateE19081 7.0ml Ready-to-UseClone: N/AImmunogen: Internal region of human Fn14.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, PlacentaCellular Localization: MembraneFOXO1A Rabbit PolyclonalCatalog No. Size FormatE17820 0.1ml ConcentrateE17822 0.5ml ConcentrateE17824 1.0ml ConcentrateE17821 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human FOXO1A.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: Cytoplasm, NucleusHuman Placenta stained withanti-Fn14 antibody E1908Human Prostate Carcinoma stained withanti-FOXO1A antibody E1782Product Description: Fibroblast growth factor-inducible 14 (Fn14) is the receptor forTweak, a member of the TNF receptor family. It was first described as a weak inducer ofapoptosis in some cell types. It is thought to promote angiogenesis and the proliferationof endothelial cells as well as modulating cellular adhesion to matrix proteins. Fn14 ishighly expressed in glioma and some cancer cases of breast, pancreas, and liver.Product Description: FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a memberof a subfamily of the forkhead homeotic gene family of transcription factors. Recentstudies have shown that this protein can act as either a coactivator or a corepressor ofnuclear receptor activity. This action is mediated through the LXXLL motif found in theC-terminus of the FOXO1A protein. Chromosomal aberrations involving FOXO1A areassociated with rhabdomyosarcoma 2 (RMS2) or alveolar rhabdomyosarcoma.FOXA1 Rabbit MonoclonalCatalog No. Size FormatM3880 0.1ml ConcentrateM3882 0.5ml ConcentrateM3884 1.0ml ConcentrateM3881 7.0ml Ready-to-UseClone: SP88Immunogen: Synthetic peptide derived from nearC-terminus of human FOXA1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ProstateCellular Localization: Nucleus in Breast Carcinoma,Prostate. Cytoplasm in most malignancies.FOXO3A Rabbit PolyclonalCatalog No. Size FormatE17730 0.1ml ConcentrateE17732 0.5ml ConcentrateE17734 1.0ml ConcentrateE17731 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human FOXO3A protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilCellular Localization: Cytoplasm, NucleusHuman Prostate stained withanti-FOXA1 antibody M388Human Breast Carcinoma stained withanti-FOXO3A antibody E1773Product Description: This gene encodes a member of the forkhead class of DNA-bindingproteins. These hepatocyte nuclear factors are transcriptional activators for liver-specifictranscripts such as albumin and transthyretin, and they also interact with chromatin.Similar family members in mice have roles in the regulation of metabolism and in thedifferentiation of the pancreas and liver.Product Description: FOXO3A belongs to the forkhead family of transcription factorswhich are characterized by a distinct forkhead domain. This gene likely functions as atrigger for apoptosis through expression of genes necessary for cell death. Translocationof this gene with the MLL gene is associated with secondary acute leukemia.Alternatively spliced transcript variants encoding the same protein have been observed.FOXA1 Rabbit PolyclonalCatalog No. Size FormatE18850 0.1ml ConcentrateE18852 0.5ml ConcentrateE18854 1.0ml ConcentrateE18851 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from nearC-terminus of human FOXA1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusFOXP1 Rabbit PolyclonalCatalog No. Size FormatE19060 0.1ml ConcentrateE19062 0.5ml ConcentrateE19064 1.0ml ConcentrateE19061 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide near C-terminus ofhuman FOXP1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Breast Carcinoma stained withanti-FOXA1 antibody E1885Human Tonsil stained withanti-FOXP1 antibody E1906Product Description: This gene encodes a member of the forkhead class of DNA-bindingproteins. These hepatocyte nuclear factors are transcriptional activators for liver-specifictranscripts such as albumin and transthyretin, and they also interact with chromatin.Similar family members in mice have roles in the regulation of metabolism and in thedifferentiation of the pancreas and liver.Product Description: Forkhead box (FOX) proteins are a group of 43 conservedtranscription factors that are further divided into subgroups. The FOXP subgroup hasfour members, FOXP1 to FOXP4, which are widely expressed and contribute somecritical roles in organ development, immune response, and cancer pathogenesis. FOXP1is a transcriptional repressor that plays an important role in lung, cardiac valve, and B celldevelopment.52Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesFOXP3 Rabbit MonoclonalFPGS Rabbit PolyclonalCatalog No. Size FormatClone: SP97Catalog No. Size FormatClone: N/AM3970 0.1ml ConcentrateM3972 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human FOXP3 protein.E18340 0.1ml ConcentrateE18342 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding tointernal region of human FPGS.M3974 1.0ml ConcentrateApplication: IHC-PE18344 1.0ml ConcentrateApplication: IHC-PM3971 7.0ml Ready-to-UseSpecies Reactivity: Human*E18341 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Breast Carcinoma, Liver CarcinomaCellular Localization: NucleusCellular Localization: CytoplasmHuman Tonsil stained withanti-FOXP3 antibody M397Human Breast Carcinoma stained withanti-FPGS antibody E1834Product Description: Forkhead box protein P3 (FOXP3) is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 is essential for normal immunehomeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25+ andCD4+ T cells, at low level in CD4+/CD25- cells, and is absent in CD4-/CD8+ T cells. FOXP-3may be a master regulatory gene and a more specific marker of regulatory T cells thanother T cells.Product Description: Folylpoly-gamma-glutamate synthetase (FPGS) converts folates topolyglutamate derivatives by catalyzing the addition of several equivalents of glutamicacid to the gamma-carboxyl group in the side chain of folate cofactors and analogs.FFPGS is widely expressed in human tumors and is tightly linked either to proliferationor to a lack of differentiation.FOXP3 Rabbit PolyclonalFRA-2 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18490 0.1ml ConcentrateE18492 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human FOXP3 protein.E4210 0.1ml ConcentrateE4212 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromN-terminus of human FRA-2 protein.E18494 1.0ml ConcentrateApplication: IHC-PE4214 1.0ml ConcentrateApplication: IHC-PE18491 7.0ml Ready-to-UseSpecies Reactivity: Human*E4211 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Prostate CarcinomaCellular Localization: NucleusCellular Localization: NucleusHuman Tonsil stained withanti-FOXP3 antibody E1849Human Prostate Carcinoma stained withanti-FRA-2 antibody E421Product Description: Forkhead box protein P3 (FOXP3) is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 is essential for normal immunehomeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25+ andCD4+ T cells, at low level in CD4+/CD25- cells, and is absent in CD4-/CD8+ T cells. FOXP-3may be a master regulatory gene and a more specific marker of regulatory T cells thanother T cells.Product Description: FRA-2 has homologous sequence with c-FOS, FRA-1, and FOS B.It forms heterodimers with c-Jun that recognize specific DNA sequences, such as thebinding site for transcription factor AP-1.FOXP4 Rabbit PolyclonalCatalog No. Size FormatE16390 0.1ml ConcentrateE16392 0.5ml ConcentrateE16394 1.0ml ConcentrateE16391 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human FOXP4 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: NucleusFragile Histidine Triad (FHIT) Rabbit PolyclonalCatalog No. Size FormatE3640 0.1ml ConcentrateE3642 0.5ml ConcentrateE3644 1.0ml ConcentrateE3641 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant full length humanFHIT protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BreastCellular Localization: CytoplasmHuman Lung stained withanti-FOXP4 antibody E1639Human Breast stained withanti-Fragile Histidine Triad (FHIT) antibody E364Product Description: FOXP4 gene belongs to subfamily P of the forkhead box (FOX)transcription factor family. Forkhead box transcription factors play important roles in theregulation of tissue- and cell type-specific gene transcription during both developmentand adulthood. Many members of the forkhead box gene family, including membersof subfamily P, have roles in mammalian oncogenesis. This gene may play a role in thedevelopment of tumors of the kidney and larynx.Product Description: This antibody reacts with human FHIT (Fragile Histidine Triad).Alterations and deletions of the FHIT gene are strongly linked to the genesis andestablishment of human tumors of the lung, cervix, breast, colon, stomach, andpancreas. In normal cells, FHIT may act as a tumor suppressor.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 53

GADD153 Rabbit PolyclonalGastrin-1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE10980 0.1ml ConcentrateE10982 0.5ml ConcentrateImmunogen: Recombinant human full lengthGADD153 protein.E4490 0.1ml ConcentrateE4492 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromN-terminus of gastrin-1.E10984 1.0ml ConcentrateApplication: IHC-PE4494 1.0ml ConcentrateApplication: IHC-PE10981 7.0ml Ready-to-UseSpecies Reactivity: Human*E4491 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: StomachCellular Localization: CytoplasmCellular Localization: CytoplasmHuman Breast Carcinoma stainedwith anti-GADD153 antibody E1098Human Stomach stained withanti-Gastrin-1 antibody E449Product Description: GADD153 has been described as a growth arrest and DNAdamage-inducible gene that encodes a C/EBP-related nuclear protein. GADD153expression is induced by a variety of cellular stresses. GADD153 functions to blockcells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBPproteins to direct GADD153 dimers away from classical C/EBP binding sites.Product Description: Gastrin is a 17 amino acid peptide hormone and neurotransmitterwidely distributed throughout the gastrointestinal (GI) tract and the central nervoussystem (CNS). Antibodies that react specifically with gastrin may be used to study thedifferential tissue expression and intracellular and subcellular localization of gastrin inneuroendocrine cells of the gastrointestinal tract, and in the CNS.Gamma Glutamylcysteine Synthetase (GCS) Rabbit PolyclonalCatalog No. Size FormatE16740 0.1ml ConcentrateE16742 0.5ml ConcentrateE16744 1.0ml ConcentrateE16741 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to internal region of ratheavy subunit of GCS protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, TestisCellular Localization: CytoplasmControl Lysate: Human KidneyMolecular Weight: 73 kDaHuman Testis stained with anti-GammaGlutamylcysteine Synthetase (GCS) antibody E1674GDNF Rabbit PolyclonalCatalog No. Size FormatE11020 0.1ml ConcentrateE11022 0.5ml ConcentrateE11024 1.0ml ConcentrateE11021 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa186-205 of human GDNF.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain (Hippocampus)Cellular Localization: CytoplasmHuman Brain stained withanti-GDNF antibody E1102Product Description: Gamma Glutamylcysteune Synthetase (GCS) is the first enzymeand rate limiting step in the glutathione biosynthesis pathway. Glutathione (GSH) playsan important role in detoxification of oxidants and toxins from the cells. It is composedof heavy and light subunits. These subunits are coded by different genes which arecontrolled by different mechanisms. The heavy subunit carries the catalytic activity andcan be inhibited through feedback mechanism by GSH.Product Description: Glial cell line-derived neurotrophic factor (GDNF) has beenidentified as a potent neurotrophic factor that enhances survival of midbraindopaminergic neurons. GDNF promotes the survival and morphological differentiationof dopaminergic neurons and increases their high-affinity dopamine uptake. It wassuggested that GDNF may have utility in the treatment of Parkinson’s disease, which ismarked by progressive degeneration of midbrain dopaminergic neurons.GANP Rabbit PolyclonalCatalog No. Size FormatE14080 0.1ml ConcentrateE14082 0.5ml ConcentrateE14084 1.0ml ConcentrateE14081 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 1-150of human GANP.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusGlial Fibrillary Acidic Protein (GFAP) Rabbit MonoclonalCatalog No. Size FormatM3780 0.1ml ConcentrateM3782 0.5ml ConcentrateM3784 1.0ml ConcentrateM3781 7.0ml Ready-to-UseClone: SP78Immunogen: Synthetic peptide corresponding toC-terminus of human GFAP protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AstrocytomaCellular Localization: CytoplasmHuman Tonsil stained withanti-GANP antibody E1408Human Astrocytoma stained withanti-Glial Fibrillary Acidic Protein (GFAP) antibody M378Product Description: Germinal center-associated nuclear protein (GANP) is thoughtto be involved in the nuclear localization pathway of MCM3 and in regulation of cellproliferation of antigen-driven B cells in germinal centers. It carries an MCM-bindingdomain in the carboxyl-terminal side and it is up-regulated selectively ingerminal centers.Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e.,glial cells) and ependymal cells of the central nervous system. The antibody to GFAP isuseful for documenting astrocytic differentiation in tumors outside the CNS.54Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesGlial Fibrillary Acidic Protein (GFAP) Rabbit PolyclonalCatalog No. Size FormatE18320 0.1ml ConcentrateE18322 0.5ml ConcentrateE18324 1.0ml ConcentrateE18321 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human GFAP.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AstrocytomaCellular Localization: CytoplasmGluR1 Rabbit PolyclonalCatalog No. Size FormatE3060 0.1ml ConcentrateE3062 0.5ml ConcentrateE3064 1.0ml ConcentrateE3061 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman GluR1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmHuman Astrocytoma stained withanti-Glial Fibrillary Acidic Protein (GFAP) antibody E1832Human Brain stained withanti-GluR1 antibody E306Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e.,glial cells) and ependymal cells of the central nervous system. The antibody to GFAP isuseful for documenting astrocytic differentiation in tumors outside the CNS.Product Description: Glutamate receptors constitute the principal excitatoryneurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropicreceptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomericcomplexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gatedcalcium channels. Metabotropic glutamate receptors are G-protein coupled receptorsand they activate phopholipases or adenylylcyclase once they are activated.Glial Fibrillary Acidic Protein (GFAP) Mouse MonoclonalCatalog No. Size Format Clone: SPM507E16510 0.1ml Concentrate Immunogen: Porcupine spinal cord.E16512 0.5ml Concentrate Application: IHC-PE16514 1.0ml ConcentrateSpecies Reactivity: Human*E16511 7.0ml Ready-to-UseIsotype: Mouse IgG1Control Tissue: Astrocytoma, BrainCellular Localization: CytoplasmGluR2/3 Rabbit PolyclonalCatalog No. Size FormatE3000 0.1ml ConcentrateE3002 0.5ml ConcentrateE3004 1.0ml ConcentrateE3001 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman GluR2/3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: CerebellumCellular Localization: CytoplasmHuman Astrocytoma stained withanti-Glial Fibrillary Acidic Protein (GFAP) antibody E1651Human Cerebellum stained withanti-GluR2/3 antibody E300Product Description: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e.,glial cells) and ependymal cells of the central nervous system. The antibody to GFAP isuseful for documenting astrocytic differentiation in tumors outside the CNS.Product Description: Glutamate receptors constitute the principal excitatoryneurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropicreceptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomericcomplexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gatedcalcium channels. Metabotropic glutamate receptors are G protein coupled receptorsand they activate phopholipases or adenylylcyclase once they are activated.Glucagon Rabbit PolyclonalGluR6/7 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18180 0.1ml ConcentrateE18182 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding tointernal region of human glucagon.E2930 0.1ml ConcentrateE2932 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman GluR6/7.E18184 1.0ml ConcentrateApplication: IHC-PE2934 1.0ml ConcentrateApplication: IHC-PE18181 7.0ml Ready-to-UseSpecies Reactivity: Human*E2931 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: PancreasControl Tissue: BrainCellular Localization: CytoplasmCellular Localization: CytoplasmHuman Pancreas stained withanti-Glucagon antibody E1818Human Brain stained withanti-GluR6/7 antibody E293Product Description: Glucagon is a hormone that is secreted by alpha cells in thepancreas. Glucagon antagonizes insulin by converting glycogen to glucose in theliver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-likepeptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylatecyclase activating polypeptide) are in the glucagons hormone family.Product Description: Glutamate receptors constitute the principal excitatoryneurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropicreceptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomericcomplexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gatedcalcium channels. Metabotropic glutamate receptors are G-protein coupled receptorsand they activate phopholipases or adenylylcyclase once they are activated.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 55

GLUT-1 Rabbit PolyclonalCatalog No. Size FormatE2840 0.1ml ConcentrateE2842 0.5ml ConcentrateE2844 1.0ml ConcentrateE2841 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human GLUT-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, EsophagusCellular Localization: MembraneGlutamic Acid Decarboxylase 65 (GAD65) Rabbit PolyclonalCatalog No. Size FormatE3310 0.1ml ConcentrateE3312 0.5ml ConcentrateE3314 1.0ml ConcentrateE3311 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman GAD65.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmHuman Esophagus stained withanti-GLUT-1 antibody E284Human Pancreas stained withanti-Glutamic Acid Decarboxylase 65 (GAD65) antibody E331Product Description: Glucose is fundamental to the metabolism of mammalian cells.Several glucose transporter protein (Glut) isoforms have been identified and shownto function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable inmany human tissues including those of the colon, lung, stomach, esophagus, and breast.Product Description: Glutamate decarboxylase 2 (GAD65) catalyzes the productionof γ-Aminobutyric acid (GABA). Adults express two forms of glutamate decarboxylase(GAD), GAD65 and GAD67. Both are expressed in most GABA containing neurons. GAD65appears to be targeted to the membrane and nerve endings; GAD65 is predominantlyexpressed in pancreatic beta cells. Autoantibodies to GAD65 are strongly associated withinsulin-dependent diabetes mellitus.GLUT-1 Mouse MonoclonalCatalog No. Size FormatE13810 0.1ml ConcentrateE13812 0.5ml ConcentrateE13814 1.0ml ConcentrateE13811 7.0ml Ready-to-UseClone: SPM498Immunogen: Synthetic peptide derived fromC-terminus of human GLUT-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG2a/kControl Tissue: Breast Carcinoma, EsophagusCellular Localization: MembraneGlutamic Acid Decarboxylase 67 (GAD67) Rabbit PolyclonalCatalog No. Size FormatE10260 0.1ml ConcentrateE10262 0.5ml ConcentrateE10264 1.0ml ConcentrateE10261 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from near N-terminusof mouse GAD67 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain, PancreasCellular Localization: Cytoplasm, MembraneHuman Esophagus stained withanti-GLUT-1 antibody E1381Human Pancreas stained withanti-Glutamic Acid Decarboxylase 67 (GAD67) antibody E1026Product Description: Glucose is fundamental to the metabolism of mammalian cells.Several glucose transporter protein (Glut) isoforms have been identified and shownto function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable inmany human tissues including those of the colon, lung, stomach, esophagus, and breast.Product Description: Glutamic Acid Decarboxylase (GAD) catalyzes the conversionof L-glutamate to gamma-aminobutyric acid (GABA), the principal inhibitoryneurotransmitter in the brain, and a putative paracrine signal molecule in pancreaticislets. GAD67 is a cytoplasmic protein and is encoded on chromosome 2.GLUT-3 Rabbit PolyclonalCatalog No. Size FormatE3270 0.1ml ConcentrateE3272 0.5ml ConcentrateE3274 1.0ml ConcentrateE3271 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human GLUT-3 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-GLUT-3 antibody E327Glypican-3 Rabbit MonoclonalCatalog No. Size Format Clone: SP86M3860 0.1ml Concentrate Immunogen: Synthetic peptide derivedM3862 0.5ml Concentrate from C-terminus of human glypican-3M3864 1.0ml Concentrate protein.M3861 7.0ml Ready-to-Use Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: MembraneControl Lysate: HepG2Molecular Weight: 64 kDaHuman Placenta stained withanti-Glypican-3 antibody M386Product Description: Glucose is fundamental to the metabolism of mammalian cells.Several glucose transporter protein (GLUT) isoforms have been identified and shown tofunction in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a fewnormal cell type spermatids in testis with active spermatogenesis, placental trophoblastmembranes, and neurons in brain. GLUT-3 staining is also detectable in human cancersincluding those of the ovary, lung, and testis.Product Description: Expression alteration of glypican-3 (GPC3) is associated withseveral malignancies and has been identified as an overexpressed gene in hepatocellularcarcinoma (HCC). Glypican-3 is a cell surface proteoglycan that bears heparan sulfate(HS). It may be involved in the suppression/modulation of growth in the predominantlymesodermal tissues and organs.56Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesGlypican-3 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18870 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE18872 0.5ml Concentrate C-terminus of human glypican-3 protein.E18874 1.0ml Concentrate Application: IHC-PE18871 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver CarcinomaCellular Localization: Cytoplasm, MembraneGranzyme B Rabbit PolyclonalCatalog No. Size FormatE2580 0.1ml ConcentrateE2582 0.5ml ConcentrateE2584 1.0ml ConcentrateE2581 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman granzyme B.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmHuman Liver Carcinoma stained withanti-Glypican-3 antibody E1887Human Tonsil stained withanti-Granzyme B antibody E258Product Description: Expression alteration of glypican-3 (GPC3) is associated withseveral malignancies and has been identified as an overexpressed gene in hepatocellularcarcinoma (HCC). Glypican-3 is a cell surface proteoglycan that bears heparan sulfate(HS). It may be involved in the suppression/modulation of growth in the predominantlymesodermal tissues and organs.Product Description: Granzyme A and granzyme B are serine proteases that mediateapoptotic signaling in cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. Bothgranzyme A and granzyme B are synthesized as inactive proenzymes, and are storedwithin cytolytic granules and released by effector cells during degradation. This antibodyshould be useful for the localization of granzyme B-containing lytic granules and for thecharacterization of activated CTLs or NK cells.GNB2 Rabbit PolyclonalCatalog No. Size FormatE17940 0.1ml ConcentrateE17942 0.5ml ConcentrateE17944 1.0ml ConcentrateE17941 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from near N-terminusof human GNB2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: MembraneGRB2-Associated Binder 1 (GAB-1) Rabbit PolyclonalCatalog No. Size FormatE3620 0.1ml ConcentrateE3622 0.5ml ConcentrateE3624 1.0ml ConcentrateE3621 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human GAB-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AstrocytomaCellular Localization: CytoplasmHuman Placenta stained withanti-GNB2 antibody E1794Human Astrocytoma stained withanti-GRB2-Associated Binder 1 (GAB-1) antibody E362Product Description: Guanine nucleotide-binding protein subunit beta-2 (GNB2) is oneof the three subunits (alpha, beta and gamma) for guanine nucleotide binding proteins(G proteins). G proteins are membrane associated and heterotrimeric proteins. Gproteins and their receptors (GPCRs) form one of the most prevalent signaling systems inmammalian cells. G protein signaling system regulates sensory perception, cell growth,and hormonal regulation.Product Description: GAB-1 is a multisubstrate docking protein downstream in thesignaling pathways of different receptor tyrosine kinases, including the epidermalgrowth factor receptor (EGFR) and appears to play a crucial role in cellular growthresponse, transformation, and apoptosis. Experimental data indicates that GAB1coupling to the Met receptor requires binding of GRB2 and correlates with thetransforming potential of Tpr-Met.GP200 (Renal Cell Carcinoma Marker) Mouse MonoclonalCatalog No. Size Format Clone: SPM487E11050 0.1ml Concentrate Immunogen: Recombinant human gp200 protein.E11052 0.5ml Concentrate Application: IHC-PE11054 1.0ml ConcentrateSpecies Reactivity: Human*E11051 7.0ml Ready-to-UseIsotype: Mouse IgG1/kControl Tissue: Kidney, Renal Cell CarcinomaCellular Localization: Cytoplasm, MembraneGRP75 Rabbit PolyclonalCatalog No. Size FormatE12490 0.1ml ConcentrateE12492 0.5ml ConcentrateE12494 1.0ml ConcentrateE12491 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa661-679 of human GRP75 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmHuman Renal Cell Carcinoma stained withanti-GP200 (Renal Cell Carcinoma Marker) antibody E1105Human Colon Carcinoma stained withanti-GRP75 antibody E1249Product Description: GP200 is localized along the brush border of renal tubule andBowman’s capsule, the luminal surfaces of breast lobules and ducts and the epididymaltubular epithelium, cytoplasm of parathyroid parenchymal cells and the colloid ofthyroid follicles. GP200 is reportedly expressed by 93% of primary and 84% of metastaticrenal cell carcinomas.Product Description: The HSP70 family is composed of four highly conserved proteins:HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75expression is restricted to the mitochondrial matrix and aids in the translocation andfolding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 57

GRP78 Rabbit PolyclonalGSK-3 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE12570 0.1ml ConcentrateE12572 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding to nearN-terminus of human GRP78 protein.E2910 0.1ml ConcentrateE2912 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman GSK-3.E12574 1.0ml ConcentrateApplication: IHC-PE2914 1.0ml ConcentrateApplication: IHC-PE12571 7.0ml Ready-to-UseSpecies Reactivity: Human*E2911 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: BrainCellular Localization: CytoplasmCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-GRP78 antibody E1257Human Brain stained withanti-GSK-3 antibody E291Product Description: The HSP70 family is composed of four highly conserved proteins:HSP70, HSC70, GRP75 and GRP78. GRP78 is localized in the endoplasmic reticulumwhere it receives imported secretory proteins and is involved in the folding andtranslocation of nascent peptide chains.Product Description: Glycogen Synthase Kinase-3 (GSK-3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways.Two isoforms exist, GSK-3α and GSK-3β. The function of GSK-3 is to phosphorylateglycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinasepathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.GRP94 Rabbit PolyclonalCatalog No. Size FormatE13860 0.1ml ConcentrateE13862 0.5ml ConcentrateE13864 1.0ml ConcentrateE13861 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to C-terminus of humanGRP94 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 94 kDaHuman Breast Carcinoma stained withanti-GRP94 antibody E1386Guanylyl Cyclase beta Rabbit PolyclonalCatalog No. Size FormatE10270 0.1ml ConcentrateE10272 0.5ml ConcentrateE10274 1.0ml ConcentrateE10271 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human guanylyl cyclase beta 1, soluble.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmHuman Brain stained withanti-Guanylyl Cyclase beta antibody E1027Product Description: Glucose-regulated protein 94kDa (GRP-94), also known asendoplasmin, is a member of human heat shock protein (HSP) 90 family. GRP-94 is acytosolic protein that participates in signaling pathways with PKC ε to protect cells fromexternal damage, particularly in heat shock-mediated events. GRP-94 localizes to the ERand is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated inhuman endothelial cells in response to hypoxia by HIF-1.Product Description: Soluble guanylyl cyclase catalyses in a Mg2+ or Mn2+ dependentmanner, the conversion of guanosine-5’-triphosphate (GTP) to cyclic guanosine-3’,5-monophosphate (cGMP) and pyrophosphate. This enzyme is a major physiologicalreceptor for nitric oxide (NO), an important intra- and intercellular membrane-permeantsignaling molecule.GRSF-1 Rabbit PolyclonalCatalog No. Size FormatE18690 0.1ml ConcentrateE18692 0.5ml ConcentrateE18694 1.0ml ConcentrateE18691 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human GRSF-1 protein.Application: IHC-PSpecies Reactivity: Human*H. Pylori Rabbit PolyclonalCatalog No. Size FormatE3010 0.1ml ConcentrateE3012 0.5ml ConcentrateE3014 1.0ml ConcentrateE3011 7.0ml Ready-to-UseClone: N/AImmunogen: Purified Helicobacter pylori.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: H. Pylori Infected TissueControl Tissue: BrainCellular Localization: H. PyloricCellular Localization: CytoplasmHuman Brain stained withanti-GRSF-1 antibody E1869Human H. Pylori infected Stomach stained withanti-H. Pylori antibody E301Product Description: G-rich sequence factor 1 (GRSF-1) is a cytoplasmic protein thatbinds RNAs containing the G-rich element. GRSF-1 is up-regulated with prostate cancerprogression and down regulated by the differentiating agents phenylacetate andphenylbutyrate. The function of GRSF-1 is uncharacterized, but potential roles includemRNA transport, stabilization, and translation. It might be involved in prostate cancermetastasis.Product Description: Helicobacter pylori (H. Pylori) is known to cause peptic ulcers andchronic gastritis in human. It is associated with duodenal ulcers and may be involvedin development of adenocarcimona and low grade lymphoma of mucosa-associatedlymphoid tissue in the stomach.58Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesH. Pylori Mouse MonoclonalCatalog No. Size FormatE18380 0.1ml ConcentrateE18382 0.5ml ConcentrateE18384 1.0ml ConcentrateE18381 7.0ml Ready-to-UseClone: SPM526Immunogen: Purified helicobacter pylori.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: H. Pylori Infected TissueCellular Localization: H. PyloricHeat Shock Protein 60 (HSP60) Rabbit PolyclonalCatalog No. Size FormatE12450 0.1ml ConcentrateE12452 0.5ml ConcentrateE12454 1.0ml ConcentrateE12451 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide correspondingto aa 31-50 of human HSP60 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 60 kDaHuman H. Pylori infected Stomach stained withanti-H. Pylori antibody E1838Human Colon Carcinoma stained withanti-Heat Shock Protein 60 (HSP60) antibody E1245Product Description: Helicobacter pylori (H. Pylori) is known to cause peptic ulcers andchronic gastritis in human. It is associated with duodenal ulcers and may be involvedin development of adenocarcimona and low grade lymphoma of mucosa-associatedlymphoid tissue in the stomach.Product Description: A wide variety of environmental and pathophysiological stressfulcondition triggers the synthesis of heat shock proteins or stress response proteins.HSP60 is a potential antigen in a number of autoimmune diseases includinghuman arthritis.HDAC1 Rabbit PolyclonalCatalog No. Size FormatE3550 0.1ml ConcentrateE3552 0.5ml ConcentrateE3554 1.0ml ConcentrateE3551 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human HDAC1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilCellular Localization: NucleusHuman Breast Carcinoma stained withanti-HDAC1 antibody E355Heat Shock Protein 70 (HSP70) Rabbit PolyclonalCatalog No. Size FormatE12330 0.1ml ConcentrateE12332 0.5ml ConcentrateE12334 1.0ml ConcentrateE12331 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to aa 574-591 of humanHSP70 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusControl Lysate: 293TMolecular Weight: 70 kDaHuman Breast Carcinoma stained withanti-Heat Shock Protein 70 (HSP70) antibody E1233Product Description: Mammalian HADC1 (also designated HD1), HDAC2 (alsodesignated mammalian RPD3) and HDAC3, all of which are related to the yeasttranscriptional regulator Rpd3p, have been identified as histone deacetylases. Thedeacetylation of histones is associated with transcriptional silencing.Product Description: The HSP70 family is composed of four highly conserved proteins:HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles: they actas molecular chaperones facilitating the assembly of multi-protein complexes, theyparticipate in the translocation of polypeptides across cell membranes and to thenucleus and they aid in the proper folding of nascent polypeptide chain. HSP70expression is strongly induced in response to heat stress.HDAC2 Rabbit PolyclonalhENT1** Rabbit MonoclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: SP120E10640 0.1ml ConcentrateE10642 0.5ml ConcentrateImmunogen: Synthetic peptide fromC-terminus of human HDAC2.M4200 0.1ml ConcentrateM4202 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human hENT1 protein.E10644 1.0ml ConcentrateApplication: IHC-P, WBM4204 1.0ml ConcentrateApplication: IHC-PE10641 7.0ml Ready-to-UseSpecies Reactivity: Human*M4201 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Pancreas, TonsilCellular Localization: NucleusCellular Localization: MembraneControl Lysate: K562Molecular Weight: 60 kDaHuman Tonsil stained withanti-HDAC2 antibody E1064Human Pancreas stained withanti-hENT1 antibody M420Product Description: Histone acetylation is a dynamic process whose levels aredetermined by the net activities of histone acetyltransferases (HATs) and the competingenzymes histone deacetylases (HDACs). Both activities are associated with the nuclearmatrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2and several other HDACs are the catalytic subunits of different multiprotein regulatorycomplexes.Product Description: Human equilibrative nucleoside transporter 1 (hENT1) is a memberof the equilibrative nucleoside transporter family. It is a transmembrane glycoproteinin the plasma and mitochondrial membranes and mediates the cellular uptake ofnucleosides. Nucleoside transporters such as hENT1 are required for nucleotidesynthesis in cells that lack de novo nucleoside synthesis pathways. These transportersare necessary for the uptake of cytotoxic nucleosides used for chemotherapies.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 59

HMGA2 Rabbit PolyclonalID3 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE16330 0.1ml ConcentrateE16332 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman HMGA2.E14050 0.1ml ConcentrateE14052 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human ID3 protein.E16334 1.0ml ConcentrateApplication: IHC-PE14054 1.0ml ConcentrateApplication: IHC-PE16331 7.0ml Ready-to-UseSpecies Reactivity: Human*E14051 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: Breast CarcinomaCellular Localization: NucleusCellular Localization: NucleusHuman Breast Carcinoma stained withanti-HMGA2 antibody E1633Human Breast Carcinoma stained withanti-ID3 antibody E1405Product Description: HMGA2, previously known as HMGI-C, belongs to the groupof high mobility chromosomal proteins that have the AT-hook DNA-binding motif.It is highly and ubiquitously expressed during embryogenesis. Chromosomalrearrangements of the HMGA2 gene and overexpression are frequently associated withbenign and malignant tumors, respectively. Human and mouse HMGA2 share 95%amino acid sequence identity.Product Description: DNA-binding protein inhibitor ID-3 (ID3) is a basic helix-loophelix(bHLH) protein that inhibits muscle-specific gene expression by the binding ofE2A-containing protein complexes to the muscle creatine kinase E-box enhancer. Underconditions that facilitate muscle cell differentiation, the Id protein levels fall, allowingE12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activatemyogenic differentiation.Human Growth Hormone (HGH) Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17470 0.1ml Concentrate Immunogen: Purified human growth hormone.E17472 0.5ml Concentrate Application: IHC-PE17474 1.0ml ConcentrateSpecies Reactivity: Human*E17471 7.0ml Ready-to-UseIsotype: Rabbit IgGControl Tissue: Anterior PituitaryCellular Localization: CytoplasmIKAROS Rabbit MonoclonalCatalog No. Size FormatM4080 0.1ml ConcentrateM4082 0.5ml ConcentrateM4084 1.0ml ConcentrateM4081 7.0ml Ready-to-UseClone: SP108Immunogen: Synthetic peptide near the N-terminus ofhuman IKAROS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Anterior Pituitary stained withanti-Human Growth Hormone (HGH) antibody E1747Human Tonsil stained withanti-IKAROS antibody M408Product Description: HGH is produced by the somatotroph cells in the pituitary.HGH acts through binding to membrane receptors. Ligand binding induces receptordimerization and activation of the receptor-associated kinase. This results inphosphorylation of the kinase itself, of the receptor, and of many cellular proteinsincluding the Stat proteins as well as adaptors leading to the activation of the Ras/MAPkinase pathway and of the PI-3 kinase pathway.Product Description: DNA-binding protein IKAROS functions in the specification andthe maturation of the T lymphocyte. It also interacts with a critical control element in theterminal deoxynucleotidyl transferase promoter as well as with the promoters for othergenes expressed during early stages of B cell and T cell development.ID2 Rabbit PolyclonalIKAROS Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE16410 0.1ml ConcentrateE16412 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman ID2 protein.E19000 0.1ml ConcentrateE19002 0.5ml ConcentrateImmunogen: Synthetic peptide derived from nearN-terminus of human IKAROS protein.E16414 1.0ml ConcentrateApplication: IHC-PE19004 1.0ml ConcentrateApplication: IHC-PE16411 7.0ml Ready-to-UseSpecies Reactivity: Human*E19001 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: TonsilCellular Localization: NucleusCellular Localization: NucleusHuman Breast Carcinoma stained withanti-ID2 antibody E1641Human Tonsil stained withanti-IKAROS antibody E1900Product Description: Inhibitor of differentiation 2 (ID2) protein is a transcriptionalmodulator. ID2 participates in the differentiation and cell cycle progression in organisms.It belongs to a protein family characterized by a helix-loop-helix (HLH) region, butlacking the basic amino acid domain required to bind to DNA. Id2 can disrupt theantiproliferative effects of retinoblastoma (Rb) family tumor suppressor proteins andfacilitate the progression of the cell cycle.Product Description: DNA-binding protein IKAROS functions in the specification andthe maturation of the T lymphocyte. It also interacts with a critical control element in theterminal deoxynucleotidyl transferase promoter as well as with the promoters for othergenes expressed during early stages of B cell and T cell development.60Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesInhibin-alpha Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17390 0.1ml Concentrate Immunogen: Synthetic peptide derivedE17392 0.5ml Concentrate from N-terminus of human inhibin-alpha.E17394 1.0ml Concentrate Application: IHC-P, WBE17391 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TestisCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 40 kDaIPO-38 (Proliferation Marker) Mouse MonoclonalCatalog No. Size FormatE16580 0.1ml ConcentrateE16582 0.5ml ConcentrateE16584 1.0ml ConcentrateE16581 7.0ml Ready-to-UseClone: SPM515Immunogen: Spleen cells of a patient with hairy cellleukemia.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgM/kControl Tissue: TonsilCellular Localization: NucleusHuman Testis stained withanti-Inhibin-alpha antibody E1739Human Tonsil stained withanti-IPO-38 (Proliferation Marker) antibody E1658Product Description: Inhibin is a dimeric glycoprotein hormone from the TGF-betafamily made up of alpha and beta subunits. It inhibits the production of folliclestimulatinghormone from the pituitary while activin stimulates the production of FSH.It is suggested that inhibin may act as a gonadal tumor suppressor.Product Description: IPO-38 antigen is present in the nuclei of proliferating cells such asHodgkin’s disease and non-Hodgkin’s lymphomas, different forms of leukemias, breastand colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in thecells of non-stimulated lymphocytes and granulocytes.Integrin beta 5 Rabbit PolyclonalCatalog No. Size FormatE2860 0.1ml ConcentrateE2862 0.5ml ConcentrateE2864 1.0ml ConcentrateE2861 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman integrin beta 5 subunit.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bone Marrow, Colon Carcinoma, SmallIntestineCellular Localization: Cytoplasm, MembraneIRS-1 Rabbit PolyclonalCatalog No. Size FormatE12590 0.1ml ConcentrateE12592 0.5ml ConcentrateE12594 1.0ml ConcentrateE12591 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa1223-1242 of human IRS-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmHuman Small Intestine stained withanti-Integrin beta 5 antibody E286Human Colon Carcinoma stained withanti-IRS-1 antibody E1259Product Description: Integrins are heterodimers composed of noncovalently associatedtransmembrane α and β subunits. Most integrin receptors bind ligands that arecomponents of the extracellular matrix, including fibronectin, collagen and vitronectin.Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors.In addition to mediating cell adhesion and cytoskeletal organization, integrins functionas signaling receptors.Product Description: The insulin receptor substrate-1 (IRS-1), a 170-185kDa proteinmajor substrate of the insulin receptor is phosphorylated in response to stimulationof cells by insulin, insulin-like growth factor (IGF-I) and interleukin 4. IRS-1 isphosphorylated on serine, threonine and tyrosine residues in a variety of tissues.Involucrin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10630 0.1ml Concentrate Immunogen: Recombinant protein encoding aaE10632 0.5ml Concentrate 420-578 of human involucrin.E10634 1.0ml Concentrate Application: IHC-PE10631 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SkinCellular Localization: CytoplasmJAB1 Rabbit PolyclonalCatalog No. Size FormatE10280 0.1ml ConcentrateE10282 0.5ml ConcentrateE10284 1.0ml ConcentrateE10281 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman JAB1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusHuman Skin stained withanti-Involucrin antibody E1063Human Breast Carcinoma stained withanti-JAB1 antibody E1028Product Description: Involucrin is a cytoplasmic protein precursor of the epidermalcornified envelope that becomes cross-linked by transglutaminase during envelopeassembly. Involucrin is expressed in a range of stratified squamous epithelia, includingthe cornea which lacks a distinct cornified layer. The protein is a useful marker ofterminal differentiation. It is tightly linked to the onset of differentiation and firstexpressed in the immediate suprabasal layers of the epidermis.Product Description: Jun activation domain-binding protein-1 (JAB1) also designatedCOP9 subunits 5 (COPS5) or SGN5 is a coactivator of AP-1 transcription factor that alsopromotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interactswith c-Jun AP-1 containing complexes, and enhances transactivation from AP-1-dependent promoters. JAB1 is highly conserved in evolution and is widely expressed inmammalian tissues. It is localized both in the nucleus and the cytoplasm.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 61

J-chain Rabbit MonoclonalCatalog No. Size FormatM4050 0.1ml ConcentrateM4052 0.5ml ConcentrateM4054 1.0ml ConcentrateM4051 7.0ml Ready-to-UseClone: SP105Immunogen: Synthetic peptide near the internal regionof human J-chain.Application: IHC-PSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmKappa Light Chain (B Cell Marker) Mouse MonoclonalCatalog No. Size Format Clone: SPM508E16520 0.1ml Concentrate Immunogen: B-lymphoma cells.E16522 0.5ml Concentrate Application: IHC-P, WBE16524 1.0ml ConcentrateSpecies Reactivity: Human*E16521 7.0ml Ready-to-UseIsotype: Mouse IgG1Control Tissue: TonsilCellular Localization: CytoplasmControl Lysate: Human SpleenMolecular Weight: 25 kDaHuman Tonsil stained withanti-J-chain antibody M405Human Tonsil stained withanti-Kappa Light Chain (B Cell Marker) antibody E1652Product Description: J chain is a small glycopeptide and is structurally unrelated toheavy or light chains, but is synthesized by all plasma cells. J chain is linked to IgA andIgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells.B cells secrete J chain at an early stage of differentiation. J chains are present in a largeproportion of the immunoglobulin-positive cells in the germinal centers of the tonsiland lymph node.Product Description: Immunoglobulins have similar structures of two identical heavychains and two identical light chains. Both the heavy chains and the light chains aredivided into constant and variable regions. The constant regions have the same aminoacid sequences between all the immunoglobulin classes. The variable regions haveapproximately 110 amino acids with high sequence variability. Kappa light chaincontains one immunoglobulin like domain.J-chain Rabbit PolyclonalKeratin 5 Rabbit MonoclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: SP27E19010 0.1ml ConcentrateE19012 0.5ml ConcentrateImmunogen: Synthetic peptide derived from nearC-terminus of human J-chain protein.M3270 0.1ml ConcentrateM3272 0.5ml ConcentrateImmunogen: Synthetic peptide fromC-terminus of human cytokeratin 5.E19014 1.0ml ConcentrateApplication: IHC-PM3274 1.0ml ConcentrateApplication: IHC-P, WBE19011 7.0ml Ready-to-UseSpecies Reactivity: Human*M3271 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmControl Tissue: Breast, Mesothelioma,Prostate, SkinCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 58 kDaHuman Tonsil stained withanti-J-chain antibody E1901Human Prostate Adenocarcinoma stained withanti-Keratin 5 antibody M327Product Description: J chain is a small glycopeptide and is structurally unrelated toheavy or light chains, but is synthesized by all plasma cells. J chain is linked to IgA andIgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells.B cells secrete J chain at an early stage of differentiation. J chains are present in a largeproportion of the immunoglobulin-positive cells in the germinal centers of the tonsiland lymph node.Product Description: Twenty human keratins are divided into acidic and basicsubfamilies. Members of the acidic and basic subfamilies are found together in pairs. Thecomposition of keratin pairs varies with the epithelial cell type, stage of differentiation,cellular growth environment, and disease state. Human keratins are useful markers incancer research. Cytokeratin 5 is expressed in breast myoepithelia cells, prostate basalcells, most epithelial and biphasic mesotheliomas.Jena-Muenchen4 (JM4) Rabbit PolyclonalCatalog No. Size FormatE14000 0.1ml ConcentrateE14002 0.5ml ConcentrateE14004 1.0ml ConcentrateE14001 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa165-178 of human JM4.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: Cytoplasm, MembraneKeratin 5 Rabbit PolyclonalCatalog No. Size FormatE13820 0.1ml ConcentrateE13822 0.5ml ConcentrateE13824 1.0ml ConcentrateE13821 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human cytokeratin 5.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, Mesothelioma, Prostate, SkinCellular Localization: CytoplasmHuman Brain stained withanti-Jena-Muenchen4 (JM4) antibody E1400Human Skin stained withanti-Keratin 5 antibody E1382Product Description: JM4 is a 19kDa four-transmembrane protein binding to the CCR5receptor. It plays a role in the regulation of intracellular protein transport. It is expressedin normal brain, small intestine, lung, spleen, and pancreas and has also been detectedin tumors of breast, colon, lung, and ovary.Product Description: Twenty human keratins are divided into acidic and basicsubfamilies. Members of the acidic and basic subfamilies are found together in pairs. Thecomposition of keratin pairs varies with the epithelial cell type, stage of differentiation,cellular growth environment, and disease state. Human keratins are useful markers incancer research. Cytokeratin 5 is expressed in breast myoepithelia cells, prostate basalcells, most epithelial and biphasic mesotheliomas.62Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesKeratin 6 Rabbit MonoclonalKeratin 7 Rabbit PolyclonalCatalog No. Size FormatClone: SP87Catalog No. Size FormatClone: N/AM3870 0.1ml ConcentrateM3872 0.5ml ConcentrateImmunogen: Synthetic peptide derivedfrom C-terminus of human keratin 6 protein.E18000 0.1ml ConcentrateE18002 0.5ml ConcentrateImmunogen: Synthetic peptide correspondingto C-terminus of human cytokeratin 7.M3874 1.0ml ConcentrateApplication: IHC-P, WBE18004 1.0ml ConcentrateApplication: IHC-P, WBM3871 7.0ml Ready-to-UseSpecies Reactivity: Human*E18001 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Prostate, SkinCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 60 kDaControl Tissue: Breast Carcinoma, OvarianCarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 54 kDaHuman Prostate Carcinoma stained withanti-Keratin 6 antibody M387Human Breast Carcinoma stained withanti-Keratin 7 antibody E1800Product Description: Cytokeratin 6 (CK6) and 16 (CK16) are expressed in keratinocytes,which undergo rapid turnover in the suprabasal region. CK6 is found in basal cells ofprostate glands, hair follicles, suprabasal cells of a variety of internal stratified epitheliaand in epidermis. Epidermal injury results in activation of keratinocytes which expressCK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cellcarcinomas.Product Description: Cytokeratin 7 (CK7) is a basic cytokeratin which is found inmost glandular and transitional epithelia but not in the stratified squamous epithelia.Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not ofcolon, prostate, or gastrointestinal tract.Keratin 6 Rabbit PolyclonalCatalog No. Size FormatE18880 0.1ml ConcentrateE18882 0.5ml ConcentrateE18884 1.0ml ConcentrateE18881 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cytokeratin 6 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, SkinCellular Localization: CytoplasmKeratin 8 Rabbit MonoclonalCatalog No. Size FormatM4020 0.1ml ConcentrateM4022 0.5ml ConcentrateM4024 1.0ml ConcentrateM4021 7.0ml Ready-to-UseClone: SP102Immunogen: Synthetic peptide corresponding toC-terminus of human keratin 8.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Ductal CarcinomaCellular Localization: Cytoplasm, MembraneHuman Prostate stained withanti-Keratin 6 antibody E1888Human Breast Ductal Carcinoma stained withanti-Keratin 8 antibody M402Product Description: Cytokeratin 6 (CK6) and 16 (CK16) are expressed in keratinocytes,which undergo rapid turnover in the suprabasal region. CK6 is found in basal cells ofprostate glands, hair follicles, suprabasal cells of a variety of internal stratified epitheliaand in epidermis. Epidermal injury results in activation of keratinocytes which expressCK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cellcarcinomas.Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecularweight keratins and exists in association with keratin 18. Keratin 8 is primarily foundin the non-squamous epithelia. It is present in hepatocellular carcinomas and themajority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cellcarcinomas.Keratin 7** Rabbit MonoclonalCatalog No. Size FormatM3520 0.1ml ConcentrateM3522 0.5ml ConcentrateM3524 1.0ml ConcentrateM3521 7.0ml Ready-to-UseClone: SP52Immunogen: Synthetic peptide correspondingto C-terminus of human cytokeratin 7.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, OvarianCarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 54 kDaHuman Breast Carcinoma stained withanti-Keratin 7 antibody M352Keratin 8 Rabbit PolyclonalCatalog No. Size FormatE3260 0.1ml ConcentrateE3262 0.5ml ConcentrateE3264 1.0ml ConcentrateE3261 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant proteinencoding aa 1-483 of human cytokeratin 8.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Lung, SkinCellular Localization: Cytoplasm,MembraneControl Lysate: HeLaMolecular Weight: 52 kDaHuman Lung stained withanti-Keratin 8 antibody E326Product Description: Cytokeratin 7 (CK7) is a basic cytokeratin which is found inmost glandular and transitional epithelia but not in the stratified squamous epithelia.Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not incolon, prostate, or gastrointestinal tract.Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecularweight keratins and exists in association with keratin 18. Keratin 8 is primarily foundin the non-squamous epithelia. It is present in hepatocellular carcinomas and themajority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cellcarcinomas.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 63

Keratin 8 Rabbit PolyclonalCatalog No. Size FormatE18400 0.1ml ConcentrateE18402 0.5ml ConcentrateE18404 1.0ml ConcentrateE18401 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from thehuman cytokeratin 8.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Colon CarcinomaCellular Localization: Cytoplasm, MembraneHuman Colon Carcinoma stained withanti-Keratin 8 antibody E1840Keratin 14 Rabbit MonoclonalCatalog No. Size FormatM3530 0.1ml ConcentrateM3532 0.5ml ConcentrateM3534 1.0ml ConcentrateM3531 7.0ml Ready-to-UseClone: SP53Immunogen: Synthetic peptide corresponding toC-terminus of human cytokeratin 14.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, Skin, SquamousCell CarcinomaCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 50 kDaHuman Prostate stained withanti-Keratin 14 antibody M353Product Description: Keratin 8 belongs to the type B (basic) subfamily of high molecularweight keratins and exists in association with keratin 18. Keratin 8 is primarily foundin the non-squamous epithelia. It is present in hepatocellular carcinomas and themajority of adenocarcinomas and ductal carcinomas, but it is absent in squamous cellcarcinomas.Product Description: Cytokeratin 14 (CK14) belongs to the type A (acidic) subfamily oflow molecular weight keratins and exists in combination with cytokeratin 5.Keratin 10** Rabbit MonoclonalCatalog No. Size FormatM3990 0.1ml ConcentrateM3992 0.5ml ConcentrateM3994 1.0ml ConcentrateM3991 7.0ml Ready-to-UseClone: SP99Immunogen: Synthetic peptide corresponding toC-terminus of human keratin 10.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SkinCellular Localization: CytoplasmKeratin 14 Rabbit PolyclonalCatalog No. Size FormatE2620 0.1ml ConcentrateE2622 0.5ml ConcentrateE2624 1.0ml ConcentrateE2621 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cytokeratin 14.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Skin, Squamous Cell CarcinomaCellular Localization: CytoplasmHuman Skin stained withanti-Keratin 10 antibody M399Human Skin stained withanti-Keratin 14 antibody E262Product Description: Cytokeratin 10 is a type I cytokeratin, expressed by suprabasalcells of stratified and cornified epithelia. Its defects are implicated in bullous congenitalichthyosiform erythroderma, epidermolytic hyperkeratosis and diffuse nonepidermolyticpalomoplantar keratoderma.Product Description: Cytokeratin 14 (CK14) belongs to the type A (acidic) subfamily oflow molecular weight keratins and exists in combination with cytokeratin 5.Keratin 10 Rabbit PolyclonalCatalog No. Size FormatE18980 0.1ml ConcentrateE18982 0.5ml ConcentrateE18984 1.0ml ConcentrateE18981 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human keratin 10 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SkinCellular Localization: CytoplasmHuman Skin stained withanti-Keratin 10 antibody E1898Keratin 17** Rabbit MonoclonalCatalog No. Size FormatM3950 0.1ml ConcentrateM3952 0.5ml ConcentrateM3954 1.0ml ConcentrateM3951 7.0ml Ready-to-UseClone: SP95Immunogen: Synthetic peptidecorresponding to C-terminus of humankeratin 17 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 48 kDaHuman Prostate Carcinoma stained withanti-Keratin 17 antibody M395Product Description: Cytokeratin 10 is a type I cytokeratin, expressed by suprabasalcells of stratified and cornified epithelia. Its defects are implicated in bullous congenitalichthyosiform erythroderma, epidermolytic hyperkeratosis and diffuse nonepidermolyticpalomoplantar keratoderma.Product Description: Cytokeratin 17 (CK17) plays a role in the formation andmaintenance of various skin appendages cytokeratin 17 is expressed in the outer rootsheath and medulla region of hair follicle from mucosal stratified squamous epithelia,basal cells of oral epithelium, palmoplantar epidermis, and sweat and mammary glands.It is also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambialcells of sebaceous gland and in exocervix.64Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesKeratin 17 Rabbit PolyclonalCatalog No. Size FormatE18920 0.1ml ConcentrateE18922 0.5ml ConcentrateE18924 1.0ml ConcentrateE18921 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom C-terminus of cytokeratin 17 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung Adenocarcinoma,Prostate CarcinomaCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 48 kDaHuman Lung Adenocarcinoma stained withanti-Keratin 17 antibody E1892Keratin 18 Mouse MonoclonalCatalog No. Size FormatE17010 0.1ml ConcentrateE17012 0.5ml ConcentrateE17014 1.0ml ConcentrateE17011 7.0ml Ready-to-UseClone: DC10Immunogen: Human breast cancer PMC42cells.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Breast Carcinoma, SkinCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 48 kDaHuman Breast Carcinoma stained withanti-Keratin 18 antibody E1701Product Description: Cytokeratin 17 (CK17) plays a role in the formation andmaintenance of various skin appendages cytokeratin 17 is expressed in the outer rootsheath and medulla region of hair follicle from mucosal stratified squamous epithelia,basal cells of oral epithelium, palmoplantar epidermis, and sweat and mammary glands.It is also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambialcells of sebaceous gland and in exocervix.Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic)subfamily of low molecular weight keratins exists in combination with cytokeratin 8. Itwas reported that tissues from gastrointestinal tract are positive for both cytokeratin 8and 18 but do not contain cytokeratin 14.Keratin 18 Rabbit MonoclonalCatalog No. Size FormatM3690 0.1ml ConcentrateM3692 0.5ml ConcentrateM3694 1.0ml ConcentrateM3691 7.0ml Ready-to-UseClone: SP69Immunogen: Synthetic peptide correspondingto C-terminus of human cytokeratin 18.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 48 kDaKeratin 18 Mouse MonoclonalCatalog No. Size Format Clone: SPM510E16540 0.1ml Concentrate Immunogen: Human breast cancer PMC42 cellsE16542 0.5ml Concentrate Application: IHC-PE16544 1.0ml ConcentrateSpecies Reactivity: Human*E16541 7.0ml Ready-to-UseIsotype: Mouse IgG1Control Tissue: Breast Carcinoma, SkinCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Keratin 18 antibody M369Human Skin stained withanti-Keratin 18 antibody E1654Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic)subfamily of low molecular weight keratins exists in combination with cytokeratin 8. Itwas reported that tissues from gastrointestinal tract are positive for both cytokeratin 8and 18 but do not contain cytokeratin 14.Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic)subfamily of low molecular weight keratins exists in combination with cytokeratin 8. Itwas reported that tissues from gastrointestinal tract are positive for both cytokeratin 8and 18 but do not contain cytokeratin 14.Keratin 18 Rabbit PolyclonalCatalog No. Size FormatE18330 0.1ml ConcentrateE18332 0.5ml ConcentrateE18334 1.0ml ConcentrateE18331 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide correspondingto C-terminus of human cytokeratin 18Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, SkinCellular Localization: CytoplasmControl Lysate: A431Molecular Weight: 48 kDaKeratin 19 Rabbit PolyclonalCatalog No. Size FormatE2630 0.1ml ConcentrateE2632 0.5ml ConcentrateE2634 1.0ml ConcentrateE2631 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human cytokeratin 19.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SkinCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Keratin 18 antibody E1833Human Skin stained withanti-Keratin 19 antibody E263Product Description: Cytokeratin 18 (CK18), which belongs to the type A (acidic)subfamily of low molecular weight keratins exists in combination with cytokeratin 8. Itwas reported that tissues from gastrointestinal tract are positive for both cytokeratin 8and 18 but do not contain cytokeratin 14.Product Description: Cytokeratin 19 (CK19) is a member of type I acidic subfamily ofintermediate filaments. It is expressed in various different human tissues except in liver.Cytokeratin 19 is not expressed in hepatocytes.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 65

Keratin 20** Rabbit MonoclonalCatalog No. Size FormatM3330 0.1ml ConcentrateM3332 0.5ml ConcentrateM3334 1.0ml ConcentrateM3331 7.0ml Ready-to-UseClone: SP33Immunogen: Synthetic peptide from C-terminus ofhuman cytokeratin 20.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmKi-67 Rabbit PolyclonalCatalog No. Size FormatE1870 0.1ml ConcentrateE1872 0.5ml ConcentrateE1874 1.0ml ConcentrateE1871 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from thehuman Ki-67 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Lymph Node, TonsilCellular Localization: NucleusHuman Colon Carcinoma stained withanti-Keratin 20 antibody M333Human Tonsil stained withanti-Ki-67 antibody E187Product Description: Cytokeratin 20 (CK20) is a Type-I keratin primarily expressedin gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressedin adenocarcinomas of the colon, stomach, pancreas and the bile system, mucinousovarian tumors, transitional-cell, and Merkel-cell carcinomas. CK20 is negative insquamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium,non-mucinous tumors of the ovary, and small cell carcinomas.Product Description: Ki-67 is a nuclear protein which is expressed in the proliferatingcells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cellcycle, while cells in the G0 (quiescent) phase are negative for this protein.Keratin 20 Rabbit PolyclonalCatalog No. Size FormatE16440 0.1ml ConcentrateE16442 0.5ml ConcentrateE16444 1.0ml ConcentrateE16441 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human cytokeratin 20.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmKOC/L523S Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18310 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toE18312 0.5ml Concentrate internal region of human KOC/L523S.E18314 1.0ml Concentrate Application: IHC-PE18311 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Placenta, Prostate CarcinomaCellular Localization: CytoplasmHuman Colon Carcinoma stained withanti-Keratin 20 antibody E1644Human Prostate Carcinoma stained withanti-KOC/L523S antibody E1831Product Description: Cytokeratin 20 (CK20) is a Type-I keratin primarily expressedin gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressedin adenocarcinomas of the colon, stomach, pancreas and the bile system, mucinousovarian tumors, transitional-cell, and Merkel-cell carcinomas. CK20 is negative insquamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium,non-mucinous tumors of the ovary, and small cell carcinomas.Product Description: Insulin-like growth factor-II messenger RNA (mRNA)-bindingprotein-3 (IMP-3), also known as K homology domain-containing protein (KOC), isa member of the insulin-like growth factor-II mRNA-binding protein family and isexpressed in some malignancies such as melanoma but not in benign nevi. IMP-3 isexpressed in a significantly higher proportion of melanomas than Spitz nevi. IMP-3appears to be involved in the progression of malignant melanoma.Ki-67 Rabbit Monoclonalk-Ras Rabbit PolyclonalCatalog No. Size FormatClone: SP6Catalog No. Size FormatClone: N/AM3060 0.1ml ConcentrateM3062 0.5ml ConcentrateImmunogen: Synthetic peptide from C-terminus ofhuman Ki-67 protein.E17960 0.1ml ConcentrateE17962 0.5ml ConcentrateImmunogen: Synthetic peptide from internal region ofhuman k-Ras protein.M3064 1.0ml ConcentrateApplication: IHC-PE17964 1.0ml ConcentrateApplication: IHC-PM3061 7.0ml Ready-to-UseSpecies Reactivity: Human*, Mouse*E17961 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Bladder, Breast, Prostate CarcinomaCellular Localization: NucleusCellular Localization: MembraneHuman Tonsil stained withanti-Ki-67 antibody M306Human Prostate Carcinoma stained withanti-k-Ras antibodyy E1796Product Description: Ki-67 is a nuclear protein which is expressed in the proliferatingcells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cellcycle, while cells in the G0 (quiescent) phase are negative for this protein.Product Description: The guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras)cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosinekinases and G-protein-coupled receptors activate Ras. GTPase-activating proteins(GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors,point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The mostcommon oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D).66Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesKu80 Rabbit PolyclonalLamin B Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE19120 0.1ml ConcentrateE19122 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human Ku80 protein.E13490 0.1ml ConcentrateE13492 0.5ml ConcentrateImmunogen: Synthetic peptide correspondingto C-terminus of human lamin B.E19124 1.0ml ConcentrateApplication: IHC-PE13494 1.0ml ConcentrateApplication: IHC-P, WBE19121 7.0ml Ready-to-UseSpecies Reactivity: Human*E13491 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: PlacentaControl Tissue: Breast CarcinomaCellular Localization: NucleusCellular Localization: Nucleus EnvelopeControl Lysate: HepG2Molecular Weight: 67 kDaHuman Placenta stained withanti-Ku80 antibody E1912Human Breast Carcinoma stained withanti-Lamin B antibody E1349Product Description: X-ray repair cross-complementing protein 5 (XRCC5), alternativename (Ku80), is a single stranded DNA-dependent ATP-dependent helicase. Ku80, alongwith Ku70 forms a heterodimeric protein and Ku80 is a widely expressed nuclear proteinthat binds to and stabilizes the ends of DNA at telomeres or double-stranded DNAbreaks. The inhibition of Ku80 can damage the DNA repair pathway of tumor cells andpotentially increase the killing of cancer cells.Product Description: Nuclear lamins form a network of filaments at the nucleoplasmicsite of the nuclear membrane. Two main subtypes of nuclear lamins can bedistinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set ofthree proteins arising from the same gene by alternative splicing: lamin A, lamin C andlamin Adel10. While the B-type lamins include two proteins arising from two distinctgenes: lamin B1 and lamin B2.Ku80 Rabbit PolyclonalLewis A Mouse MonoclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: SPM522E19110 0.1ml ConcentrateE19112 0.5ml ConcentrateE19114 1.0ml ConcentrateE19111 7.0ml Ready-to-UseImmunogen: Synthetic peptide derived fromC-terminus of human Ku80 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: NucleusE17380 0.1ml ConcentrateE17382 0.5ml ConcentrateE17384 1.0ml ConcentrateE17381 7.0ml Ready-to-UseImmunogen: Mucin isolated from an ovarian cyst.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Colon CarcinomaCellular Localization: CytoplasmHuman Placenta stained withanti-Ku80 antibody E1911Human Colon Carcinoma stained withanti-Lewis A antibody E1738Product Description: X-ray repair cross-complementing protein 5 (XRCC5), alternativename (Ku80), is a single stranded DNA-dependent ATP-dependent helicase. Ku80, alongwith Ku70 forms a heterodimeric protein and Ku80 is a widely expressed nuclear proteinthat binds to and stabilizes the ends of DNA at telomeres or double-stranded DNAbreaks. The inhibition of Ku80 can damage the DNA repair pathway of tumor cells andpotentially increase the killing of cancer cells.Product Description: Lewis A is a blood group antigen consisting of Gal Beta1-3 (Fucalpha1-4) GlcNAc. Lewis A is found to be present primarily on epithelial cells such ascolon and kidneys.Lambda Light Chain Rabbit PolyclonalCatalog No. Size FormatE19200 0.1ml ConcentrateE19202 0.5ml ConcentrateE19204 1.0ml ConcentrateE19201 7.0ml Ready-to-UseClone: N/AImmunogen: Free lambda light chain frompooled human IgG lambda myeloma sera.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: Cytoplasm, MembraneControl Lysate: Human SpleenMolecular Weight: 25 kDaLIMD1 Rabbit PolyclonalCatalog No. Size FormatE18700 0.1ml ConcentrateE18702 0.5ml ConcentrateE18704 1.0ml ConcentrateE18701 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human LIMD1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung, Lung CarcinomaCellular Localization: Cytoplasm, NucleusHuman Tonsil stained withanti-Lambda Light Chain antibody E1920Human Lung Carcinoma stained withanti-LIMD1 antibody E1870Product Description: Lambda light chains are polypeptide chains located in the cellmembrane and cytoplasmic regions of normal B cells and plasma cells. The combinationof lambda light chains and heavy chains forms immunoglobulin molecules. There aretwo classes of light chains found in immunoglobulins: kappa light chains and lambdalight chains. Light chain production by lymphoid cells is genetically restricted to singlelight chain class.Product Description: The Zyxin family of proteins contains five members: Ajuba, LIMD1,LPP, TRIP6 and Zyxin. LIMD1 is a ubiquitously expressed tumor suppressor. LIM domainsconsist of a cysteine-rich consensus sequence containing two distinct zinc-bindingsubdomains, which mediate protein-protein interactions. LIMD1 was first identifiedwhen the deletion of its gene was noted in some cervical cancers. LIMD1 blocks tumorgrowth and is downregulated in lung cancer.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 67

Lipocalin-2 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18810 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearE18812 0.5ml Concentrate N-terminus of human lipocalin-2 protein.E18814 1.0ml Concentrate Application: IHC-PE18811 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Stomach CarcinomaCellular Localization: CytoplasmLMO2 Mouse MonoclonalCatalog No. Size FormatE17650 0.1ml ConcentrateE17652 0.5ml ConcentrateE17654 1.0ml ConcentrateE17651 7.0ml Ready-to-UseClone: 1A9-3D11Immunogen: Synthetic peptide corresponding tohuman LMO2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Hodgkin’s Lymphoma, TonsilCellular Localization: NucleusHuman Stomach Carcinoma stained withanti-Lipocalin-2 antibody E1881Human Tonsil stained withanti-LMO2 antibody E1765Product Description: Lipocalin 2 is a member of the lipocalin family whichencompass more than 25 members (including proteins like retinol binding protein, a1microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). Themembers of the lipocalin family are characterized by their ability to bind small lipophilicsubstances in their hydrophobic core, and thereby serve as transporters of substances.Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that isrequired for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role inhematopoietic development and is highly conserved. The LMO-2 transcription start siteis located approximately 25 kb downstream from the 11p13 T cell translocation cluster(11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.LMO2** Rabbit MonoclonalCatalog No. Size FormatM3510 0.1ml ConcentrateM3512 0.5ml ConcentrateM3514 1.0ml ConcentrateM3511 7.0ml Ready-to-UseClone: SP51Immunogen: Synthetic peptidecorresponding to near N-terminus of humanLMO2 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s Lymphoma, TonsilCellular Localization: NucleusControl Lysate: RamosMolecular Weight: 24 kDaHuman Tonsil stained withanti-LMO2 antibody M351Luteinizing Hormone (LH) Rabbit PolyclonalCatalog No. Size FormatE17480 0.1ml ConcentrateE17482 0.5ml ConcentrateE17484 1.0ml ConcentrateE17481 7.0ml Ready-to-UseClone: N/AImmunogen: Purified human luteinizing hormone.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Anterior PituitaryCellular Localization: CytoplasmHuman Anterior Pituitary stained withanti-Luteinizing Hormone (LH) antibody E1748Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that isrequired for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role inhematopoietic development and is highly conserved. The LMO-2 transcription start siteis located approximately 25 kb downstream from the 11p13 T cell translocation cluster(11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.Product Description: LH is a tropic hormone which modulates the secretory activity ofother endocrine glands. It is produced in the anterior hypophysis of the pituitary gland.The glycoprotein hormone, LH, like follicle stimulating hormone and thyroid stimulatinghormone, is composed of a common alpha-subunit but also a specific beta-subunit,which characterizes each of these hormones.LMO2 Rabbit PolyclonalMAGE-1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE17830 0.1ml ConcentrateE17832 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding tohuman LMO2 protein.E4500 0.1ml ConcentrateE4502 0.5ml ConcentrateImmunogen: Full length recombinant human MAGE-1protein.E17834 1.0ml ConcentrateApplication: IHC-PE4504 1.0ml ConcentrateApplication: IHC-PE17831 7.0ml Ready-to-UseSpecies Reactivity: Human*E4501 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Hodgkin’s Lymphoma, TonsilControl Tissue: Melanoma, TestisCellular Localization: NucleusCellular Localization: CytoplasmHuman Tonsil stained withanti-LMO2 antibody E1783Human Melanoma stained withanti-MAGE-1 antibody E450Product Description: LMO-2 encodes a cysteine-rich, two LIM-domain protein that isrequired for yolk sac erythropoiesis. The LMO-2 protein has a central and crucial role inhematopoietic development and is highly conserved. The LMO-2 transcription start siteis located approximately 25 kb downstream from the 11p13 T cell translocation cluster(11p13 ttc), where T cell acute lymphoblastic leukemia-specific translocations occur.Product Description: Melanoma-associated antigen 1 (MAGE-1) genes encode animportant group of antigens. MAGE-1 is expressed on a significant proportion of humanneoplasms such as melanoma, brain glial tumors, neuroblastoma, non-small cell lungcancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas, but not on kidneytumor, leukemias and lymphomas.68Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesMALT-1 Rabbit PolyclonalMCL-1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18900 0.1ml ConcentrateE18902 0.5ml ConcentrateImmunogen: Synthetic peptide derived frominternal region of human MALT-1 protein.E2640 0.1ml ConcentrateE2642 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human MCL-1.E18904 1.0ml ConcentrateApplication: IHC-P, WBE2644 1.0ml ConcentrateApplication: IHC-PE18901 7.0ml Ready-to-UseSpecies Reactivity: Human*E2641 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: B Cell Lymphoma, LymphNode, TonsilCellular Localization: Cytoplasm, NucleusControl Tissue: TonsilCellular Localization: Cytoplasm, NucleusControl Lysate: HeLaMolecular Weight: 90 kDaHuman B Cell Lymphoma stained withanti-MALT-1 antibody E1890Human Tonsil stained withanti-MCL-1 antibody E264Product Description: Mucosa-associated lymphoid tissue lymphoma translocationprotein 1 (MALT-1) has two alternatively spliced transcript variants encoding differentisoforms. Isoform a is protein of 824 amino acids, and isoform b is a protein of 813 aminoacids. MALT-1 is thought to play a role in NF kappaB signaling by enchancing NF kappaBactivation through interaction with BCL-10. Interaction between MALT-1 and BCL-10mediates IKK activation in vitro.Product Description: Myeloid cell leukemia-1 (MCL-1) is a member of the BCL-2 family.The carboxy terminal of MCL-1 and BCL-2 share significant sequence homology.Expression of MCL-1 is increased upon exposure of ML-1 cells to ionizing radiation,ultraviolet radiation, and alkylating drugs along with increases in GADD45 and Bax anda decrease in BCL-2. Like BCL-2, MCL-1 has the capacity to promote cell viability underconditions that otherwise cause apoptosis.Mammaglobin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE16400 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toE16402 0.5ml Concentrate human mammmaglobin protein.E16404 1.0ml Concentrate Application: IHC-PE16401 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Mammaglobin antibody E1640MCM2 Rabbit MonoclonalCatalog No. Size FormatM3850 0.1ml ConcentrateM3852 0.5ml ConcentrateM3854 1.0ml ConcentrateM3851 7.0ml Ready-to-UseClone: SP85Immunogen: Synthetic peptide derivedfrom internal region of human MCM2protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Rectal CarcinomaCellular Localization: NucleusControl Lysate: HeLaMolecular Weight: 130 kDaHuman Rectal Carcinoma stained withanti-MCM2 antibody M385Product Description: The mammaglobin gene was first identified using a differentialscreening approach directed at the isolation of novel, human breast cancer-associatedgenes. Mammaglobin encodes a 10kDa glycoprotein and is distantly related to a familyof epithelial secretory proteins. Mammaglobin is overexpressed in human breast cancer.Mammaglobin is a relatively specific mammary marker.Product Description: The mini-chromosome maintenance (MCM) family ofproteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) andMCM7(CDC47), are regulators of DNA replication that act to ensure replication occursonly once in the cell cycle. Expression of MCM proteins increases during cell growth,peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/cyclin B in vitro.MART-1/Melan-A Rabbit PolyclonalClone: N/ACatalog No. Size FormatE2830 0.1ml Concentrate Immunogen: Recombinant human MART-1 protein.E2832 0.5ml Concentrate Application: IHC-PE2834 1.0ml ConcentrateSpecies Reactivity: Human*E2831 7.0ml Ready-to-UseIsotype: Rabbit IgGControl Tissue: MelanomaCellular Localization: CytoplasmMCM2 Rabbit PolyclonalCatalog No. Size FormatE18730 0.1ml ConcentrateE18732 0.5ml ConcentrateE18734 1.0ml ConcentrateE18731 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human MCM2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Rectal CarcinomaCellular Localization: NucleusHuman Melanoma stained withanti-MART-1/Melan-A antibody E283Human Rectal Carcinoma stained withanti-MCM2 antibody E1873Product Description: MART-1 / Melan-A is a melanocyte differentiation antigenrecognized by autologous cytotoxic T lymphocytes. Seven other melanoma associatedantigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1 tyrosinase,gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 ispresent in melanosomes and endoplasmic reticulum.Product Description: The mini-chromosome maintenance (MCM) family ofproteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) andMCM7(CDC47), are regulators of DNA replication that act to ensure replication occursonly once in the cell cycle. Expression of MCM proteins increases during cell growth,peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/cyclin B in vitro.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 69

MCM2 Rabbit PolyclonalCatalog No. Size FormatE12480 0.1ml ConcentrateE12482 0.5ml ConcentrateE12484 1.0ml ConcentrateE12481 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human MCM2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusMDGI Rabbit PolyclonalCatalog No. Size FormatE18620 0.1ml ConcentrateE18622 0.5ml ConcentrateE18624 1.0ml ConcentrateE18621 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human MDGI protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-MCM2 antibody E1248Human Colon Carcinoma stained withanti-MDGI antibody E1862Product Description: The mini-chromosome maintenance (MCM) family ofproteins, including MCM2, MCM3, MCM4(CDC21), MCM5(CDC46), MCM6(Mis5) andMCM7(CDC47), are regulators of DNA replication that act to ensure replication occursonly once in the cell cycle. Expression of MCM proteins increases during cell growth,peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for CDC2/cyclin B in vitro.Product Description:MDGI/FABP3 is a small cytosolic protein responsible for thetransport and deposition of fatty acids inside the cell.MCM5 Rabbit PolyclonalCatalog No. Size FormatE13830 0.1ml ConcentrateE13832 0.5ml ConcentrateE13834 1.0ml ConcentrateE13831 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 1-150of human MCM5.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ProstateCellular Localization: NucleusMDM2 Rabbit PolyclonalCatalog No. Size FormatE3500 0.1ml ConcentrateE3502 0.5ml ConcentrateE3504 1.0ml ConcentrateE3501 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human MDM2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusHuman Prostate stained withanti-MCM5 antibody E1383Human Breast Carcinoma stained withanti-MDM2 antibody E350Product Description: MCM5 (Mini Chromosome Maintainance protein-5) is involved inthe control of DNA replication. MCM proteins have DNA dependent ATPase motifs intheir central domain which is conserved from yeast to mammals. MCM proteins forma complex which exhibits ATPase and helicase activities. G0 phase levels of MCM2 andMCM5 are lower than that of MCM7 and MCM3 but increase as the cell enters G1/Sphase of the cell cycle.Product Description: Human homolog of murine double minute-2 (MDM2) and p53function in mutual regulation: activation of p53 results in expression of MDM2 whichconsequently inhibits the ability of wild type p53 to stimulate expression of targetgenes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and toa much lesser extent in a wide variety of malignancies of bladder, breast, testis, cervix,glial cells.MDGI Rabbit PolyclonalCatalog No. Size FormatE18670 0.1ml ConcentrateE18672 0.5ml ConcentrateE18674 1.0ml ConcentrateE18671 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human MDGI protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmMEF-2 Rabbit PolyclonalCatalog No. Size FormatE12390 0.1ml ConcentrateE12392 0.5ml ConcentrateE12394 1.0ml ConcentrateE12391 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa487-507 of human MEF-2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: NucleusHuman Colon Carcinoma stained withanti-MDGI antibody E1867Human Brain stained withanti-MEF-2 antibody E1239Product Description: MDGI/FABP3 is a small cytosolic protein responsible for thetransport and deposition of fatty acids inside the cell.Product Description:The myocyte enhancer factors-2 (MEF-2) family of transcriptionfactors associate with a variety of co-repressors or co-activators to regulate developmentand function of T cells, neuronal cells, and muscle cells. Four distinct family membersarise from alternatively splices transcripts, termed MEF-2A, MEF-2B, MEF-2C, and MEF-2D. These members bind as homo- and heterodimers to the MEF2 site in the promoterregion of affected genes.70Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesMelanoma (gp100) Rabbit PolyclonalCatalog No. Size FormatE4450 0.1ml ConcentrateE4452 0.5ml ConcentrateE4454 1.0ml ConcentrateE4451 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 1-150human gp100.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: MelanomaCellular Localization: CytoplasmmGluR1 Rabbit PolyclonalCatalog No. Size FormatE2890 0.1ml ConcentrateE2892 0.5ml ConcentrateE2894 1.0ml ConcentrateE2891 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman mGluR1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmHuman Melanoma stained withanti-Melanoma (gp100) antibody E445Human Brain stained withanti-mGluR1 antibody E289Product Description: By immunohistochemistry, this antibody specifically recognizesa protein in melanocytes and melanomas. Intradermal nevi, normal adult melanocytes,and non-melanocytic cells are negative. It does not stain tumor cells of epithelial,lymphoid, glial, or mesenchymal origin.Product Description: Glutamate receptors constitute the principal excitatoryneurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropicreceptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomericcomplexes of various subunits (GluR1-7, NMDA1-3, KAI-2) which comprise ligand-gatedcalcium channels. Metabotropic glutamate receptors are G-protein coupled receptors,which when activated can activate phopholipases or adenylylcyclase.Mesothelin Rabbit MonoclonalCatalog No. Size Format Clone: SP74M3740 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toM3742 0.5ml Concentrate C-terminus of human mesothelin protein.M3744 1.0ml Concentrate Application: IHC-PM3741 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: MesotheliomaCellular Localization: MembranemGluR5 Rabbit PolyclonalCatalog No. Size FormatE2870 0.1ml ConcentrateE2872 0.5ml ConcentrateE2874 1.0ml ConcentrateE2871 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman mGluR5.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmHuman Mesothelioma stained withanti-Mesothelin antibody M374Human Brain stained withanti-mGluR5 antibody E287Product Description: Mesothelin is a glycocylphosphotidylianositol-linked cell-surfaceglycoprotein that is present on the surface of normal mesothelium and is overexpressedin epithelial ovarian cancer and malignant mesotheliomas.Product Description: Glutamate receptors constitute the principal excitatoryneurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropicreceptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomericcomplexes of various subunits (GluR1-7, NMDA1-3, KAI-2) which comprise ligand-gatedcalcium channels. Metabotropic glutamate receptors are G-protein coupled receptors,which when activated can activate phopholipases or adenylylcyclase.Mesothelin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18390 0.1ml Concentrate Immunogen: Synthetic peptide derived from theE18392 0.5ml Concentrate human mesothelin.E18394 1.0ml Concentrate Application: IHC-PE18391 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: MesotheliomaCellular Localization: MembraneMicrophthalmia (MiTF) Rabbit PolyclonalCatalog No. Size FormatE17900 0.1ml ConcentrateE17902 0.5ml ConcentrateE17904 1.0ml ConcentrateE17901 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tohuman MiTF.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: MelanomaCellular Localization: NucleusHuman Mesothelioma stained withanti-Mesothelin antibody E1839Human Melanoma stained withanti-Microphthalmia (MiTF) antibody E1790Product Description: Mesothelin is a glycocylphosphotidylianositol-linked cell-surfaceglycoprotein that is present on the surface of normal mesothelium and is overexpressedin epithelial ovarian cancer and malignant mesotheliomas.Product Description: Microphthalmia associated transcription factor (MiTF) is a basichelix-loop-helix leucine zipper transcription factor implicated in pigmentation, mastcells and bone development. There are two known isoforms of MiTF. The shorterforms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, whilethe longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16melonoma cells, as well as mast cells and heart.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 71

MIP-1 alpha Rabbit PolyclonalCatalog No. Size FormatE12680 0.1ml ConcentrateE12682 0.5ml ConcentrateE12684 1.0ml ConcentrateE12681 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human MIP-1 alpha.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: Cytoplasm, MembraneMMP-2 (72kDa Collagenase IV) Rabbit PolyclonalCatalog No. Size FormatE18010 0.1ml ConcentrateE18012 0.5ml ConcentrateE18014 1.0ml ConcentrateE18011 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from nearN-terminus of human MMP-2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bladder, Breast, Ovarian Carcinoma,PlacentaCellular Localization: CytoplasmHuman Lung stained withanti-MIP-1 alpha antibody E1268Human Placenta stained withanti-MMP-2 (72kDa Collagenase IV) antibody E1801Product Description: Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa memberof the C-C chemokine subfamily. C-C chemokines are chemoattractants and activatorsfor monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFsproduced by CD8+ T cells, along with MIP-1b and RANTES.Product Description: MMPs are proteolytic enzymes capable of degrading connectivetissue components. They have a common mode of activation, a conserved amino acidsequence in the putative metal-binding active site region, and are inhibited by specifictissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role inregulating angiogenesis. MMP-2 is activated by cleavage of the first 80 amino acids. Thisantibody reacts with both latent and active MMP-2.MLH1 Rabbit PolyclonalCatalog No. Size FormatE17810 0.1ml ConcentrateE17812 0.5ml ConcentrateE17814 1.0ml ConcentrateE17811 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human MLH1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Rectal CarcinomaCellular Localization: NucleusMMP-3 (Stromelysin-1) Rabbit PolyclonalCatalog No. Size FormatE12670 0.1ml ConcentrateE12672 0.5ml ConcentrateE12674 1.0ml ConcentrateE12671 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 251-478 of human MMP-3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Colon Carcinoma stained withanti-MLH1 antibody E1781Human Placenta stained withanti-MMP-3 (Stromelysin-1) antibody E1267Product Description: MUTL protein homolog 1 (MLH1) is a DNA mismatch repairprotein. The repair of mismatch DNA is essential to maintain the integrity of geneticinformation. An alteration of microsatellite repeats due to strand misalignment duringDNA replication is referred to as microsatellite instability (MSI). These defects in DNArepair pathways have been related to human carcinogenesis.Product Description: Matrix metalloproteinases (MMP) are proteolytic enzymes capableof degrading connective tissue components. Imbalanced secretion of certain MMP ordisturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) isimplicated in the invasive potential of malignant tumors. Stromelysin (MMP3/Transin)degrades several matrix components including the core proteins of proteoglycans,laminin and non-helical regions of collagens.MMP-1 Rabbit PolyclonalCatalog No. Size FormatE3570 0.1ml ConcentrateE3572 0.5ml ConcentrateE3574 1.0ml ConcentrateE3571 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from near C-terminus ofhuman MMP-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bladder, Breast, Ovarian Carcinoma,PlacentaCellular Localization: CytoplasmMMP-9 (92kDa Collagenase IV) Rabbit PolyclonalCatalog No. Size FormatE3660 0.1ml ConcentrateE3662 0.5ml ConcentrateE3664 1.0ml ConcentrateE3661 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from nearC-terminus of human MMP-9 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-MMP-1 antibody E357Human Placenta stained withanti-MMP-9 (92kDa Collagenase IV) antibody E366Product Description: Matrix metalloproteinases (MMP) are proteolytic enzymes capableof degrading connective tissue components. Imbalanced secretion of certain MMPor disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs)is implicated in the invasive potential of tumors. MMP-1, MMP2, and MMP-9 dissolveextracellular matrix (ECM) and may initiate and promote angiogenesis.Product Description: MMPs are proteolytic enzymes capable of degrading connectivetissue components. They have a common mode of activation, a conserved amino acidsequence in the putative metal binding-active site region, and are inhibited by specifictissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role inregulating angiogenesis. This antibody reacts with both latent and active MMP-9.72Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesMMP-10 (Stromelysin-2) Rabbit PolyclonalCatalog No. Size FormatE3670 0.1ml ConcentrateE3672 0.5ml ConcentrateE3674 1.0ml ConcentrateE3671 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from the hingedomain of human MMP-10 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: CytoplasmMMP-19 Rabbit PolyclonalCatalog No. Size FormatE4030 0.1ml ConcentrateE4032 0.5ml ConcentrateE4034 1.0ml ConcentrateE4031 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman MMP-19 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-MMP-10 (Stromelysin-2) antibody E367Human Placenta stained withanti-MMP-19 antibody E403Product Description: MMPs are proteolytic enzymes capable of degrading connectivetissue components. They have a common mode of activation, a conserved amino acidsequence in the putative metal binding-active site region, and are inhibited by specifictissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role inregulating angiogenesis. This antibody reacts with both latent and active MMP-10.Product Description: MMPs are proteolytic enzymes capable of degrading connectivetissue components. They have a common mode of activation, a conserved amino acidsequence in the putative metal binding-active site region, and are inhibited by specifictissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role inregulating angiogenesis. MMP-19 is the target of autoantibodies in rheumatoid arthritisand SLE.MMP-14/MT1-MMP Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3860 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE3862 0.5ml Concentrate region of human MMP-14 protein.E3864 1.0ml Concentrate Application: IHC-PE3861 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: CytoplasmMMP-23 Rabbit PolyclonalCatalog No. Size FormatE4040 0.1ml ConcentrateE4042 0.5ml ConcentrateE4044 1.0ml ConcentrateE4041 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman MMP-23 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-MMP-14/MT1-MMP antibody E386Human Placenta stained withanti-MMP-23 antibody E404Product Description: MMPs are a group of enzymes involved in matrix degradation.MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively,contain a C-terminus transmembrane domain anchoring them to the cell surface. Theyhave an 8 amino acid insert in their catalytic domain. MMP-14 cleaves progelatinaseA (MMP-2) and progelatinase B to their active forms and it gets activated through themembrane plasmin cascade.Product Description: MMPs are proteolytic enzymes capable of degrading connectivetissue components. They have a common mode of activation, a conserved amino acidsequence in the putative metal binding-active site region, and are inhibited by specifictissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role inregulating angiogenesis. MMP-23 transcripts have been detected in reproductive organslike ovary, testis and prostate. MMP-23 is inhibited by TIMP-1.MMP-15/MT2-MMP Rabbit PolyclonalCatalog No. Size Format Clone: N/AE4120 0.1ml Concentrate Immunogen: Synthetic peptide from the catalyticE4122 0.5ml Concentrate domain of mouse MMP-15/MT2-MMP protein.E4124 1.0ml Concentrate Application: IHC-PE4121 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmMSH2 Rabbit MonoclonalCatalog No. Size FormatM3460 0.1ml ConcentrateM3462 0.5ml ConcentrateM3464 1.0ml ConcentrateM3461 7.0ml Ready-to-UseClone: SP46Immunogen: Synthetic peptide corresponding tointernal region of human MSH2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusHuman Placenta stained withanti-MMP-15/MT2-MMP antibody E412Human Colon Carcinoma stained withanti-MSH2 antibody M346Product Description: MMPs are a group of enzymes involved in matrix degradation.MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectivelycontain a C-terminus transmembrane domain anchoring them to the cell surface.MMP15 plays a minor role in activation of pro-MMP-2 to its active form in breastcarcinomas. MMP14 and MMP15 can be coexpressed in advanced stages of breastcarcinoma.Product Description: MSH2 as a mismatch repair protein is involved in DNA repair.Mutations of MSH2 are found in approximately 50% of inherited non polyposiscolorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal,dominantly inherited disease associated with marked increase in cancer susceptibility.It is characterized by a familial predisposition to early onset colorectal carcinoma andcancers of the gastrointestinal, urological and female reproductive tracts.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 73

MSH2 Rabbit PolyclonalCatalog No. Size FormatE17790 0.1ml ConcentrateE17792 0.5ml ConcentrateE17794 1.0ml ConcentrateE17791 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human MSH2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusMSH6 Mouse MonoclonalCatalog No. Size FormatE18370 0.1ml ConcentrateE18372 0.5ml ConcentrateE18374 1.0ml ConcentrateE18371 7.0ml Ready-to-UseClone: SPM525Immunogen: Synthetic peptide corresponding tointernal region of human MSH6 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Colon CarcinomaCellular Localization: NucleusHuman Colon Carcinoma stained withanti-MSH2 antibody E1779Human Colon Carcinoma stained withanti-MSH6 antibody E1837Product Description: MSH2 as a mismatch repair protein is involved in DNA repair.Mutations of MSH2 are found in approximately 50% of inherited non polyposiscolorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal,dominantly inherited disease associated with marked increase in cancer susceptibility.It is characterized by a familial predisposition to early onset colorectal carcinoma andcancers of the gastrointestinal, urological and female reproductive tracts.Product Description: MSH6 as a mismatch repair protein is central to mismatch DNArepair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma(HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited diseaseassociated with marked increase in cancer susceptibility. It is characterized by a familialpredisposition to early onset colorectal carcinoma and cancers of the gastrointestinal,urological and female reproductive tracts.MSH3 Rabbit PolyclonalCatalog No. Size FormatE18940 0.1ml ConcentrateE18942 0.5ml ConcentrateE18944 1.0ml ConcentrateE18941 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human MSH3 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Rectal CarcinomaCellular Localization: NucleusMSH6 Rabbit PolyclonalCatalog No. Size FormatE17670 0.1ml ConcentrateE17672 0.5ml ConcentrateE17674 1.0ml ConcentrateE17671 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human MSH6 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusHuman Rectal Carcinoma stained withanti-MSH3 antibody E1894Human Colon Carcinoma stained withanti-MSH6 antibody E1767Product Description: MSH3 belongs to the MutS DNA mismatch repair family althoughits precise function in mammals is still unknown. Yeast MSH3 is thought to act in acomplex with MSH2 to recognize DNA insertion/deletion mispairs.Product Description: MSH6 as a mismatch repair protein is central to mismatch DNArepair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma(HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited diseaseassociated with marked increase in cancer susceptibility. It is characterized by a familialpredisposition to early onset colorectal carcinoma and cancers of the gastrointestinal,urological and female reproductive tracts.MSH6 Rabbit MonoclonalCatalog No. Size FormatM3930 0.1ml ConcentrateM3932 0.5ml ConcentrateM3934 1.0ml ConcentrateM3931 7.0ml Ready-to-UseClone: SP93Immunogen: Synthetic peptidecorresponding to internal region of humanMSH6 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Rectal CarcinomaCellular Localization: NucleusControl Lysate: A431Molecular Weight: 160 kDamTOR Rabbit PolyclonalCatalog No. Size FormatE18590 0.1ml ConcentrateE18592 0.5ml ConcentrateE18594 1.0ml ConcentrateE18591 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human mTOR protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Rectal Carcinoma stained withanti-MSH6 antibody M393Human Breast Carcinoma stained withanti-mTOR antibody E1859Product Description: MSH6 as a mismatch repair protein is central to mismatch DNArepair. Mutations of MSH6 are found in inherited non polyposis colorectal carcinoma(HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited diseaseassociated with marked increase in cancer susceptibility. It is characterized by a familialpredisposition to early onset colorectal carcinoma and cancers of the gastrointestinal,urological and female reproductive tracts.Product Description: Mammalian target of rapamycin (mTOR) or FKBP12-rapamycinassociated protein (FRAP) is a serine/threonine-protein kinase involved in cell cycleprogression, DNA recombination, and DNA damage detection. The MTOR-rapamycincomplex is known to inhibit progression through the G1 cell cycle stage by interferingwith mitogenic signaling pathways. The binding of FRAP to FKBP12-rapamycin iscorrelated with the ability of these ligands to inhibit cell cycle progression.74Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesmTOR Rabbit PolyclonalCatalog No. Size FormatE18450 0.1ml ConcentrateE18452 0.5ml ConcentrateE18454 1.0ml ConcentrateE18451 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromhuman mTOR.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneMUC-1/EMA/Epithelial Membrane Antigen Mouse MonoclonalCatalog No. Size Format Clone: SPM492E11190 0.1ml Concentrate Immunogen: Human breast cell line ZR-75-1.E11192 0.5ml Concentrate Application: IHC-PE11194 1.0ml ConcentrateSpecies Reactivity: Human*E11191 7.0ml Ready-to-UseIsotype: Mouse IgG1/kControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-mTOR antibody E1845Human Breast Carcinoma stained withanti-MUC-1/EMA/Epithelial Membrane Antigen antibody E1119Product Description: Mammalian target of rapamycin (mTOR) or FKBP12-rapamycinassociated protein (FRAP) is a serine/threonine-protein kinase involved in cell cycleprogression, DNA recombination, and DNA damage detection. The MTOR-rapamycincomplex is known to inhibit progression through the G1 cell cycle stage by interferingwith mitogenic signaling pathways. The binding of FRAP to FKBP12-rapamycin iscorrelated with the ability of these ligands to inhibit cell cycle progression.Product Description: MUC-1 is a heavily O-glycosylated transmembrane proteinexpressed on most secretory epithelium, including mammary gland and somehematopoietic cells. It is expressed abundantly in lactating mammary glands andoverexpressed abundantly in >90% breast carcinomas and metastases. TransgenicMUC-1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands.MUC-1 Underglycosylated Mouse MonoclonalCatalog No. Size FormatE11200 0.1ml ConcentrateE11202 0.5ml ConcentrateE11204 1.0ml ConcentrateE11201 7.0ml Ready-to-UseClone: SPM493Immunogen: Human breast cell line ZR-75-1.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm,MembraneControl Lysate: MCF7Molecular Weight: 230 kDaMucin 5AC Mouse MonoclonalCatalog No. Size FormatE11080 0.1ml ConcentrateE11082 0.5ml ConcentrateE11084 1.0ml ConcentrateE11081 7.0ml Ready-to-UseClone: 45M1Immunogen: M1 mucin preparation from the fluid of anovarian mucinous cyst.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: StomachCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-MUC-1 Underglycosylated antibody E1120Human Stomach stained withanti-Mucin 5AC antibody E1108Product Description: Epithelial membrane antigen (EMA), or CA15-3, or polymorphicepithelial mucin (PEM), or sialomucin, or episialin, is a mucin-like glycoprotein. Thisantibody stains an underglycosylated MUC1 often present on carcinoma cells.Product Description: Mucins are high molecular weight glycoproteins with 80%carbohydrate contents and the remaining 20% is constituted by protein core. Gastricmucin is found in normal human gastrointestinal tract. Resurgence of gastric mucinreactivity during colonic carcinogenesis is due to re-expression of the peptide core ofgastric (or fetal colonic) mucins.MUC-1/EMA/Epithelial Membrane Antigen Rabbit PolyclonalCatalog No. Size FormatE3540 0.1ml ConcentrateE3542 0.5ml ConcentrateE3544 1.0ml ConcentrateE3541 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from the cytoplasmictail of human MUC-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneMucin 5AC Mouse MonoclonalCatalog No. Size Format Clone: SPM488E11070 0.1ml Concentrate Immunogen: Recombinant Mucin 5AC protein.E11072 0.5ml Concentrate Application: IHC-PE11074 1.0ml ConcentrateSpecies Reactivity: Human*E11071 7.0ml Ready-to-UseIsotype: Mouse IgG1/kControl Tissue: StomachCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-MUC-1/EMA/Epithelial Membrane Antigen antibody E354Product Description: MUC-1 is a heavily O-glycosylated transmembrane proteinexpressed on most secretory epithelium, including mammary gland and somehematopoietic cells. It is expressed abundantly in lactating mammary glands andoverexpressed abundantly in >90% breast carcinomas and metastases. TransgenicMUC-1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands.Human Stomach stained withanti-Mucin 5AC antibody E1107Product Description: Mucins are high molecular weight glycoproteins with 80%carbohydrate contents and the remaining 20% is constituted by protein core. Gastricmucin is found in normal human gastrointestinal tract. Resurgence of gastric mucinreactivity during colonic carcinogenesis is due to re-expression of the peptide core ofgastric (or fetal colonic) mucins.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 75

Mucin-2/MUC-2 Mouse MonoclonalCatalog No. Size Format Clone: SPM512E16560 0.1ml Concentrate Immunogen: Synthetic peptide corresponding to a siteE16562 0.5ml Concentrate on the MUC-2 glycoprotein.E16564 1.0ml Concentrate Application: IHC-PE16561 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Small IntestineCellular Localization: Cytoplasm, MembraneMyeloperoxidase (MPO) Rabbit MonoclonalCatalog No. Size FormatM3720 0.1ml ConcentrateM3722 0.5ml ConcentrateM3724 1.0ml ConcentrateM3721 7.0ml Ready-to-UseClone: SP72Immunogen: Synthetic peptide corresponding toC-terminus of human myeloperoxidase protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmHuman Small Intestine stained withanti-Mucin-2/MUC-2 antibody E1656Human Tonsil stained withanti-Myeloperoxidase (MPO) antibody M372Product Description: Secreted epithelial mucins are large macromolecules which exhibitextreme polydispersity. Mucin-2 is the major intestinal mucin. O-glycans are attachedto MUC-2 in a potentially diverse arrangement, which is crucial for their interaction withendogeneous and exogeneous lectins.Product Description: Myeloperoxidase (MPO) is an important enzyme usedby granulocytes during phagocytic lysis. In normal tissues and in a variety ofmyeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic typesexhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes,lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is postive in myeloid,but negative in lymphoid leukemias.MUM1/IRF4 Rabbit MonoclonalCatalog No. Size Format Clone: SP114M4140 0.1ml Concentrate Immunogen: Synthetic peptide nearM4142 0.5ml Concentrate C-terminus of human MUM1/IRF4.M4144 1.0ml Concentrate Application: IHC-P, WBM4141 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s LymphomaCellular Localization: NucleusControl Lysate: RamosMolecular Weight: ~51.7 kDaMyeloperoxidase (MPO) Rabbit PolyclonalCatalog No. Size FormatE17710 0.1ml ConcentrateE17712 0.5ml ConcentrateE17714 1.0ml ConcentrateE17711 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human myeloperoxidase protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lymph Node, Spleen, TonsilCellular Localization: CytoplasmHuman Hodgkin’s Lymphoma stained withanti-MUM1/IRF4 antibody M414Human Lymph Node stained withanti-Myeloperoxidase (MPO) antibody E1771Product Description: The MUM1 (Multiple Myeloma Oncogene 1) gene was originallyidentified because of its involvement in the t(6;14) translocation observed in multiplemyeloma, which causes juxtaposition of the MUM1 gene to the Ig heavy-chain locus.MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloid andextra-hematopoitic tumors. It is an excellent marker for Reed-Sternberg cells of classicHodgkin’s disease.Product Description: Myeloperoxidase (MPO) is an important enzyme usedby granulocytes during phagocytic lysis. In normal tissues and in a variety ofmyeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic typesexhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes,lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is postive in myeloid,but negative in lymphoid leukemias.MUM1/IRF4 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18350 0.1ml Concentrate Immunogen: Recombinant protein encoding aa 144-E18352 0.5ml Concentrate 451 of human MUM1 protein.E18354 1.0ml Concentrate Application: IHC-PE18351 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hodgkin’s LymphomaCellular Localization: Cytoplasm, NucleusMyoglobin Rabbit PolyclonalCatalog No. Size FormatE2990 0.1ml ConcentrateE2992 0.5ml ConcentrateE2994 1.0ml ConcentrateE2991 7.0ml Ready-to-UseClone: N/AImmunogen: Purified human myoglobin.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Skeletal MuscleCellular Localization: CytoplasmControl Lysate: Human Skeletal MuscleMolecular Weight: 17 kDaHuman Hodgkin’s Lymphoma stained withanti-MUM1/IRF4 antibody E1835Human Skeletal Muscle stained withanti-Myoglobin antibody E299Product Description: The MUM1 (Multiple Myeloma Oncogene 1) gene was originallyidentified because of its involvement in the t(6;14) translocation observed in multiplemyeloma, which causes juxtaposition of the MUM1 gene to the Ig heavy-chain locus.MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloidand extra-hematopoitic tumors. MUM1 is a valuable marker for understanding andcharacterizing histogenesis of B-cell lymphomas.Product Description: Myoglobin is a cytoplasmic, single chain polypeptide of 153 aminoacids and contains a single heme group. Myoglobin is found in skeletal and cardiacmuscle but not in smooth muscle and functions as an oxygen transporting pigment.Upon damage to the muscle cell due to infarction of a coronary artery, neurologicaltrauma, infection of tumor processes, myoglobin escapes to the environment.76Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesNCK2 Rabbit PolyclonalNestin Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE11040 0.1ml ConcentrateE11042 0.5ml ConcentrateImmunogen: Recombinant protein encodingaa 119-276 of human NCK2.E18750 0.1ml ConcentrateE18752 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human nestin protein.E11044 1.0ml ConcentrateApplication: IHC-PE18754 1.0ml ConcentrateApplication: IHC-PE11041 7.0ml Ready-to-UseSpecies Reactivity: Human*E18751 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilControl Tissue: Liver CarcinomaCellular Localization: CytoplasmCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-NCK2 antibody E1104Human Liver Carcinoma stained withanti-Nestin antibody E1875Product Description: The NCK family of SH2/SH3 adaptor proteins consists of twomembers, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGFand PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein bindshuman PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membraneruffling and lamellipod formation.Product Description: Nestin is a class VI intermediate filament expressed in thedeveloping central nervous system (CNS) in early embryonic neuroepithelial stem cells.This protein has been widely used as a predominant marker for stem / progenitor cells,glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is asuperior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.Nephrin Rabbit PolyclonalNestin Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18160 0.1ml ConcentrateE18162 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding tointernal region of human nephrin.E18610 0.1ml ConcentrateE18612 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human nestin protein.E18164 1.0ml ConcentrateApplication: IHC-PE18614 1.0ml ConcentrateApplication: IHC-PE18161 7.0ml Ready-to-UseSpecies Reactivity: Human*E18611 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: KidneyControl Tissue: Kidney, Liver CarcinomaCellular Localization: MembraneCellular Localization: CytoplasmHuman Kidney stained withanti-Nephrin antibody E1816Human Liver Carcinoma stained withanti-Nestin antibody E1861Product Description: Nephrin, a cell adhesion molecule, may play a crucial role inmaintaining the glomerular filtration barrier. Three novel podocyte proteins, podocin,nephrin and alpha actinin 4 have been identified. The role of nephrin in anti-apoptoticactivity in podocyte is believed to be associated with vascular endothelial derivedgrowth factors VEGF signaling. Nephrin seems to play a role in the development orfunction of the kidney glomerular filtration barrier.Product Description: Nestin is a class VI intermediate filament expressed in thedeveloping central nervous system (CNS) in early embryonic neuroepithelial stem cells.This protein has been widely used as a predominant marker for stem / progenitor cells,glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is asuperior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.Nestin Rabbit MonoclonalCatalog No. Size FormatM4030 0.1ml ConcentrateM4032 0.5ml ConcentrateM4034 1.0ml ConcentrateM4031 7.0ml Ready-to-UseClone: SP103Immunogen: Synthetic peptidecorresponding to C-terminus of humannestin.Application: IHC-P, WBSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: KidneyCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 230 kDaHuman Kidney stained withanti-Nestin antibody M403Neuron Specific Enolase (NSE) Rabbit PolyclonalCatalog No. Size FormatE3300 0.1ml ConcentrateE3302 0.5ml ConcentrateE3304 1.0ml ConcentrateE3301 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human NSE.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmHuman Pancreas stained withanti-Neuron Specific Enolase (NSE) antibody E330Product Description: Nestin is a class VI intermediate filament expressed in thedeveloping central nervous system (CNS) in early embryonic neuroepithelial stem cells.This protein has been widely used as a predominant marker for stem / progenitor cells,glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is asuperior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.Product Description: Enolase is a glycolytic enzyme catalyzing the reaction pathwaybetween 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolasemolecules are dimers composed of three distinct subunits (α,β and γ). The alpha-subunitis expressed in most tissues and the beta-subunit only in muscle. The gamma-subunitis expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells.Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 77

NFkB p50 Rabbit PolyclonalCatalog No. Size FormatE3630 0.1ml ConcentrateE3632 0.5ml ConcentrateE3634 1.0ml ConcentrateE3631 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman NFκB p50.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, Testis, TonsilCellular Localization: CytoplasmNitric Oxide Synthase-Inducible (iNOS) Rabbit MonoclonalCatalog No. Size FormatM4260 0.1ml ConcentrateM4262 0.5ml ConcentrateM4264 1.0ml ConcentrateM4261 7.0ml Ready-to-UseClone: SP126Immunogen: Synthetic peptide near the C-terminus ofmouse iNOS protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: CytoplasmHuman Tonsil stained withanti-NFkB p50 antibody E363Human Lung stained withanti-Nitric Oxide Synthase-Inducible (iNOS) antibody M426Product Description: NF kappa B is a heterodimer consisting of a DNA binding subunitand a transactivating subunit (RelA). P50 subunit of NFkB has an isoform and twoproteins of 105 and 100kDa respectively. The p50/p65 heterodimer is present in thecytoplasm in active form bound to its inhibitor, IkB. Upon stimulation of cells with stimulilike LPS, IL-1, IkB gets phosphorylated and degraded by proteosomes releasing theactivated NFkB which goes to the nucleus inducing gene expression.Product Description: Inducible NOS (iNOS) or macrophage NOS (mNOS) is calcium/calmodulin independent and is expressed in activated macrophages and stimulated glialcells. NOS oxidizes a guanidine nitrogen of arginine, releasing nitric oxide in the form ofa free radical and citrulline. Nitric oxide thus generated acts as a messenger in diversefunctions including vasodilation, neurotransmission, anti-tumor and anti-pathogenicactivities.NFkB p65 Rabbit PolyclonalCatalog No. Size FormatE2750 0.1ml ConcentrateE2752 0.5ml ConcentrateE2754 1.0ml ConcentrateE2751 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internaldomain of human NFκB p65.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Placenta, ProstateCellular Localization: Cytoplasm, NucleusNitric Oxide Synthase-Inducible (iNOS) Rabbit PolyclonalCatalog No. Size FormatE3740 0.1ml ConcentrateE3742 0.5ml ConcentrateE3744 1.0ml ConcentrateE3741 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of mouse i-NOS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: CytoplasmHuman Prostate stained withanti-NFkB p65 antibody E275Human Lung stained withanti-Nitric Oxide Synthase-Inducible (iNOS) antibody E374Product Description: NF kappa B is a heterodimer consisting of a 50kDa DNA bindingsubunit and a 65kDa transactivating subunit (RelA). The p50/p65 heterodimer is presentin the cytoplasm in inactive form bound to its inhibitor, IκB. In unstimulated cells,nuclear localization signals present on p65 are masked by members of the IκB familyof inhibitory proteins. Subsequent to cell stimulation, IκB undergoes phosphorylation,ubiquitination and degradation by a proteosome-dependent pathway.Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitricoxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as amessenger in diverse functions including vasodilation neurotransmission, anti-tumorand anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) iscalcium/calmodulin independent and is expressed in activated macrophages andstimulated glial cells. This antibody reacts with iNOS.Nitric Oxide Synthase-Endothelial (eNOS) Rabbit PolyclonalCatalog No. Size FormatE4110 0.1ml ConcentrateE4112 0.5ml ConcentrateE4114 1.0ml ConcentrateE4111 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human e-NOS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmNitric Oxide Synthase-Universal (uNOS) Rabbit PolyclonalCatalog No. Size FormatE3930 0.1ml ConcentrateE3932 0.5ml ConcentrateE3934 1.0ml ConcentrateE3931 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of mouse iNOS and nNOS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-Nitric Oxide Synthase-Endothelial (eNOS) antibody E411Human Placenta stained withanti-Nitric Oxide Synthase-Universal (uNOS) antibody E393Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitricoxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as amessenger in diverse functions including vasodilation neurotransmission, anti-tumorand anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS)or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelialNOS (eNOS). This antibody reacts with eNOS.Product Description: NOS oxidizes a guanidine nitrogen of arginine releasing nitricoxide in the form of a free radical and citrulline. Nitric oxide thus generated, acts as amessenger in diverse functions including vasodilation neurotransmission, anti-tumorand anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS)or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelialNOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.78Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesNKX3.1 Rabbit PolyclonalCatalog No. Size FormatE14100 0.1ml ConcentrateE14102 0.5ml ConcentrateE14104 1.0ml ConcentrateE14101 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human NKX3.1Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, Prostate CarcinomaCellular Localization: Cytoplasm, NucleusOccludin Rabbit PolyclonalCatalog No. Size FormatE17460 0.1ml ConcentrateE17462 0.5ml ConcentrateE17464 1.0ml ConcentrateE17461 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to C-terminus of humanoccludin.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: MembraneControl Lysate: Human KidneyMolecular Weight: 65 kDaHuman Prostate stained withanti-NKX3.1 antibody E1410Human Lung stained withanti-Occludin antibody E1746Product Description: The NKX3.1 gene is an androgen regulated prostate-specifichomeobox gene, located on chromosome 8p21. In mice, the homologue NKX3.1 isexclusively expressed in prostate epithelium. In humans, NKX3.1 expression is alsorestricted to the prostate. Many data show that NKX3.1 expression is highly, but notexclusively, specific for the prostate.Product Description: Occludin can exist in a variety of phosphorylated forms, rangingup to approximately 82kDa. This phosphorylation is thought to be involved in regulatingboth the localization and the function of occludin. Polyunsaturated fatty acids areknown to up-regulate occludin expression. The level of occludin varies. In brain tissue,occludin is highly expressed at cell-cell contact sites, whereas non-neural tissues showlower expression and discontinuous distribution.Notch-1 Rabbit PolyclonalCatalog No. Size FormatE10860 0.1ml ConcentrateE10862 0.5ml ConcentrateE10864 1.0ml ConcentrateE10861 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman notch-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, Ovarian CarcinomaCellular Localization: Cytoplasm, MembraneOCT-1 Rabbit PolyclonalCatalog No. Size FormatE3840 0.1ml ConcentrateE3842 0.5ml ConcentrateE3844 1.0ml ConcentrateE3841 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human OCT-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Ovarian Carcinoma stained withanti-Notch-1 antibody E1086Human Tonsil stained withanti-OCT-1 antibody E384Product Description: The 280-330kDa Notch proteins have been found to beoverexpressed or rearranged in human tumors. Ligands for notch include Jagged,Jagged-2 and delta. Jagged can activate notch and prevent myoblast differentiation byinhibiting the expression of muscle regulatory and structural genes.Product Description: The POU dimer from the OCT-1 gene formed on the palindromicOCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit thetranscriptional coactivator OBF1. OCT-1 encodes one protein, NF-A1, which is foundin nuclear extracts from all cell types and thus is not specific to lymphoid cells as isthe protein NF-A2, which is encoded by OCT-2. The OCT-1 and OCT-2 map to humanchromosomes 1q22-q23 and 19, respectively.NRF2 Rabbit PolyclonalCatalog No. Size FormatE12500 0.1ml ConcentrateE12502 0.5ml ConcentrateE12504 1.0ml ConcentrateE12501 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human NRF2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Placenta, LungCellular Localization: Cytoplasm, NucleusOCT-2 Rabbit PolyclonalCatalog No. Size FormatE4300 0.1ml ConcentrateE4302 0.5ml ConcentrateE4304 1.0ml ConcentrateE4301 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human OCT-2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Placenta stained withanti-NRF2 antibody E1250Human Tonsil stained withanti-OCT-2 antibody E430Product Description: NRF1 (also designated TCF11), NRF2 and NRF3 are bZIPtranscription factors that heterodimerize with Maf proteins to bind MARE (Mafrecognition elements) sequences. NRF2 is widely expressed and is thought to translocateto the nucleus after treatment with xenobiotics and antioxidants, which stimulate itsrelease from a repressor protein Keap1.Product Description: OCT-2 is a transcription factor belonging to the POU homeodomainfamily that binds to the Ig gene octomer sites regulating B cell specific genes.OCT-2 protein expression is not restricted to B cells, although expression levels are muchhigher in these cells. OCT-2 expression is reported to be significantly greater in germinalcenter derived lymphomas, although other B cell lymphomas also display high levels ofexpression.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 79

OCT-3/OCT-4 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18280 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toE18282 0.5ml Concentrate internal region of human OCT-3/OCT-4 protein.E18284 1.0ml Concentrate Application: IHC-PE18281 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SeminomaCellular Localization: Nucleusp120 (catenin delta 1) Rabbit MonoclonalCatalog No. Size FormatM3630 0.1ml ConcentrateM3632 0.5ml ConcentrateM3634 1.0ml ConcentrateM3631 7.0ml Ready-to-UseClone: SP63Immunogen: Synthetic peptide from C-terminus ofhuman p120.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Seminoma stained withanti-OCT-3/OCT-4 antibody E1828Human Breast Carcinoma stained withanti-p120 (catenin delta 1) antibody M363Product Description: OCT-3 (also known as OCT-4) is a mammalian POU transcriptionfactor expressed by early embryo cells and germ cells. OCT-3/4 is essential for theidentity of the pluripotential founder cell population in the mammalian embryo. Acritical amount of OCT-3/4 is required to sustain stem cell renewal, and up or downregulation induce divergent developmental programs.Product Description: Catenin delta 1 (p120) is an efficient tyrosine kinase substrateimplicated both in cell transformation by SRC and in ligand-induced receptor signalingthrough the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins tocadherins produces a complex which is linked to the actin filament network, and whichseems to be of primary importance for the cell-adhesion properties of cadherins.OCT-3/OCT-4 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17720 0.1ml Concentrate Immunogen: Synthetic peptide corresponding toE17722 0.5ml Concentrate internal region of human OCT-3/OCT-4 protein.E17724 1.0ml Concentrate Application: IHC-PE17721 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: SeminomaCellular Localization: Nucleusp120 (catenin delta 1) Rabbit PolyclonalCatalog No. Size FormatE17530 0.1ml ConcentrateE17532 0.5ml ConcentrateE17534 1.0ml ConcentrateE17531 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human p120.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Seminoma stained withanti-OCT-3/OCT-4 antibody E1772Human Breast Carcinoma stained withanti-p120 (catenin delta 1) antibody E1753Product Description: OCT-3 (also known as OCT-4) is a mammalian POU transcriptionfactor expressed by early embryo cells and germ cells. OCT-3/4 is essential for theidentity of the pluripotential founder cell population in the mammalian embryo. Acritical amount of OCT-3/4 is required to sustain stem cell renewal, and up or downregulation induce divergent developmental programs.Product Description: Catenin delta 1 (p120) is an efficient tyrosine kinase substrateimplicated both in cell transformation by SRC and in ligand-induced receptor signalingthrough the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins tocadherins produces a complex which is linked to the actin filament network, and whichseems to be of primary importance for the cell-adhesion properties of cadherins.Osteopontin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3280 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE3282 0.5ml Concentrate domain of human osteopontin.E3284 1.0ml Concentrate Application: IHC-PE3281 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasmp14ARF Rabbit PolyclonalCatalog No. Size FormatE4350 0.1ml ConcentrateE4352 0.5ml ConcentrateE4354 1.0ml ConcentrateE4351 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human p14ARF.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LymphomaCellular Localization: NucleusHuman Breast Carcinoma stained withanti-Osteopontin antibody E328Human Lymphoma stained withanti-p14ARF antibody E435Product Description: Osteopontin is a 34kDa extracellular matrix protein with acell binding domain. It was originally identified as a major component of the noncollagenousorganic bone matrix, however, it has subsequently been demonstrated in awide range of normal adult tissue and body fluids. It is involved in bone mineralization,cell adhesion, cell migration, chronic inflammatory disease and transformation.Product Description: The INK4a-ARF locus encodes two unrelated proteins both ofwhich function in tumor suppression. p14ARF binds to mdm2 and promotes the rapiddegradation of mdm2 protein required for mdm2 modification and concurrent p53stabilization and accumulation. This interaction is mediated by the exon 1beta-encodedN-terminal domain of p14ARF and a C-terminus region of mdm2.80Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary Antibodiesp27Kip1 Rabbit PolyclonalCatalog No. Size FormatE2600 0.1ml ConcentrateE2602 0.5ml ConcentrateE2604 1.0ml ConcentrateE2601 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human p27Kip1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, ProstateCellular Localization: Nucleusp53 Rabbit PolyclonalCatalog No. Size FormatE2010 0.1ml ConcentrateE2012 0.5ml ConcentrateE2014 1.0ml ConcentrateE2011 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant human full length wild typep53 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusHuman Prostate stained withanti-p27Kip1 antibody E260Human Colon Carcinoma stained withanti-p53 antibody E201Product Description: This antibody recognizes a protein of 27kDa, identified as p27Kip1.p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulatorof G1 progression and has been proposed to function as a possible mediator of TGF-binduced G1 arrest.Product Description: p53 is a tumor suppressor gene expressed in a wide variety oftissue types and is involved in regulating cell growth, replication, and apoptosis. Itbinds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNAdamage and possibly facilitates repair. Mutation involving p53 is found in a wide varietyof malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.p300/CBP Rabbit PolyclonalCatalog No. Size FormatE14070 0.1ml ConcentrateE14072 0.5ml ConcentrateE14074 1.0ml ConcentrateE14071 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human p300.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Nucleusp53 Mouse MonoclonalCatalog No. Size FormatE16570 0.1ml ConcentrateE16572 0.5ml ConcentrateE16574 1.0ml ConcentrateE16571 7.0ml Ready-to-UseClone: SPM514Immunogen: Recombinant human p53 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG2a/kControl Tissue: Colon CarcinomaCellular Localization: NucleusHuman Breast Carcinoma stained withanti-p300/CBP antibody E1407Human Colon Carcinoma stained withanti-p53 antibody E1657Product Description: The p300-CBP is composed of two transcriptional co-activatingproteins with similar structures: p300 and CBP. Both p300 and CBP interact withnumerous transcription factors and act to increase the expression of their target genessuch as cAMP-regulated enhancer (CRE). Both contain five protein interaction domains:the nuclear receptor interaction domain, the CREB-MYB interaction domain, thecysteine/histidine regions and the interferon binding domain.Product Description: p53 is a tumor suppressor gene expressed in a wide variety oftissue types and is involved in regulating cell growth, replication, and apoptosis. Itbinds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNAdamage and possibly facilitates repair. Mutation involving p53 is found in a wide varietyof malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.p53 Rabbit MonoclonalCatalog No. Size FormatM3050 0.1ml ConcentrateM3052 0.5ml ConcentrateM3054 1.0ml ConcentrateM3051 7.0ml Ready-to-UseClone: SP5Immunogen: Recombinant human full length wild typep53 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Nucleusp57 ** Rabbit MonoclonalCatalog No. Size FormatM4180 0.1ml ConcentrateM4182 0.5ml ConcentrateM4184 1.0ml ConcentrateM4181 7.0ml Ready-to-UseClone: SP118Immunogen: Synthetic peptide corresponding to aninternal region of human p57 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: NucleusHuman Colon Carcinoma stained withanti-p53 antibody M305Human Placenta stained withanti-p57 antibody M418Product Description: p53 is a tumor suppressor gene expressed in a wide variety oftissue types and is involved in regulating cell growth, replication, and apoptosis. Itbinds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNAdamage and possibly facilitates repair. Mutation involving p53 is found in a wide varietyof malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.Product Description: Cyclin-dependent kinase inhibitor 1C (CDKN 1C or p57/Kip2) is anuclear protein and a potent inhibitor for several G1 cyclin/Cdk complexes. P57 inhibitscell proliferation and the mutation of p57 gene can lead to uncontrollable cell growthand malignancy such as hydatidiform mole.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 81

p57 Rabbit PolyclonalCatalog No. Size FormatE18990 0.1ml ConcentrateE18992 0.5ml ConcentrateE18994 1.0ml ConcentrateE18991 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human p57/kip2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Hydatid Mole, PlacentaCellular Localization: Nucleusp70S6k (Phospho T421/S424) Rabbit PolyclonalCatalog No. Size FormatE18500 0.1ml ConcentrateE18502 0.5ml ConcentrateE18504 1.0ml ConcentrateE18501 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from theregion of human p70S6K that contains phosphorylatedthreonine 421 and serine 424.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Alzheimer’s Brain, Breast CarcinomaCellular Localization: CytoplasmHuman Hydatid Mole stained withanti-p57 antibody E1899Human Alzheimer’s Brain stained withanti-p70S6k (Phospho T421/S424) antibody E1850Product Description: p57/Kip 2 (or CDKN 1C) is a potent tight binding inhibitor ofseveral G1 cyclin complexes, and is a negative regulator of cell proliferation. The geneencoding human p57/Kip 2 is located on chromosome 11p15.5, a region implicatedin both sporadic cancers, Wilm’s tumor, and Beckwith Wiedemann syndrome (BWS, acancer syndrome) making p57/Kip 2 a tumor suppressor candidate.Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka)and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with amolecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecularmass of 85kDa. Both p70 and p85 S6K1 are activated by multiple S/T phosphorylations inresponse to various mitogenic stimuli and nutrients. This antibody detects p70S6K onlywhen phosphorylated at T421/S424.p70S6k Rabbit PolyclonalPAK alpha Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18640 0.1ml ConcentrateE18642 0.5ml ConcentrateImmunogen: Synthetic peptide derived from internalregion of human p70S6k protein.E12550 0.1ml ConcentrateE12552 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding to aa525-545 of rat PAK alpha.E18644 1.0ml ConcentrateApplication: IHC-PE12554 1.0ml ConcentrateApplication: IHC-PE18641 7.0ml Ready-to-UseSpecies Reactivity: Human*E12551 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: Breast CarcinomaCellular Localization: CytoplasmCellular Localization: Cytoplasm, NucleusHuman Breast Carcinoma stained withanti-p70S6k antibody E1864Human Breast Carcinoma stained withanti-PAK alpha antibody E1255Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka)and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with amolecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecularmass of 85kDa. Both, p70 and p85 S6K1 are activated by multiple S/T phosphorylationsin response to various mitogenic stimuli and nutrients.Product Description: Alpha PAK p68, beta PAK p65 and gamma PAK p62 are threeisoforms of serine/threonine kinases. These three isoforms complex specifically withRac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activityleading to their autophosphorylation.p70S6k (Paired 421/424) Rabbit PolyclonalCatalog No. Size FormatE18510 0.1ml ConcentrateE18512 0.5ml ConcentrateE18514 1.0ml ConcentrateE18511 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from the regionof human p70S6K that contains non-phosphorylatedthreonine 421 and serine 424.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmParathyroid Hormone Rabbit PolyclonalCatalog No. Size FormatE18090 0.1ml ConcentrateE18092 0.5ml ConcentrateE18094 1.0ml ConcentrateE18091 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human parathyroid hormone.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Parathyroid GlandCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-p70S6k (Paired 421/424) antibody E1851Human Parathyroid Gland stained withanti-Parathyroid Hormone antibody E1809Product Description: Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka)and S6K2 (S6Kb). The S6K1 isoform has two splicing forms: p70S6K or p70S6K1 with amolecular mass of 70kDa and the second isoform, p85S6K or p85S6K1 with a molecularmass of 85kDa. Both p70 and p85 S6K1 are activated by multiple S/T phosphorylations inresponse to various mitogenic stimuli and nutrients. This antibody detects p70S6K that isnon-phosphorylated at T421/S424.Product Description: Parathyroid hormone (PTH) is secreted by parathyroid cells. Thishormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing theirrenal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism.Most PTH is secreted in its intact, mature 9.5kDa form, amino acids 1-84; however, itcan also be secreted as N-terminal truncated fragments or C-terminus fragments afterintracellular degradation, as in case of hypercalcemia.82Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesParathyroid Hormone Receptor 1 Rabbit PolyclonalCatalog No. Size FormatE17890 0.1ml ConcentrateE17892 0.5ml ConcentrateE17894 1.0ml ConcentrateE17891 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human parathyroid hormone receptortype 1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Parathyroid GlandCellular Localization: MembranePAX-5 Rabbit PolyclonalCatalog No. Size FormatE4390 0.1ml ConcentrateE4392 0.5ml ConcentrateE4394 1.0ml ConcentrateE4391 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human PAX-5 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Parathyroid Gland stained withanti-Parathyroid Hormone Receptor 1 antibody E1789Human Tonsil stained withanti-PAX-5 antibody E439Product Description: Two human parathyroid hormone (hPTH) receptors are known:one that binds both hPTH and a PTH-related protein called PTHrP (the Type I receptor);and one that only binds hPTH (the Type II receptor).Product Description: PAX-5 is a B cell specific activator protein. In the early stages of Bcell development, PAX-5 influences the expression of several B cell specific genes, suchas CD19 and CD20. PAX-5 is expressed primarily in pro-, pre-, and mature B cells, but notin plasma cells. PAX-5 mRNA is transiently detected in the mesencephalon and spinalcord during embryogenesis and then shifts to the fetal liver. This indicates that PAX-5 isimportant in B cell and neuronal development.Parkin Rabbit PolyclonalPaxillin Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE3470 0.1ml ConcentrateE3472 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromN-terminus of human parkin protein.E13870 0.1ml ConcentrateE13872 0.5ml ConcentrateImmunogen: Recombinant protein encoding aa356-558 of human paxillin.E3474 1.0ml ConcentrateApplication: IHC-PE13874 1.0ml ConcentrateApplication: IHC-PE3471 7.0ml Ready-to-UseSpecies Reactivity: Human*E13871 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Alzheimer’s BrainControl Tissue: Breast CarcinomaCellular Localization: CytoplasmCellular Localization: CytoplasmHuman Alzheimer’s Brain stained withanti-Parkin antibody E347Human Breast Carcinoma stained withanti-Paxillin antibody E1387Product Description: Parkin has been mapped to chromosome 6 and encodes a proteinof 465 amino acids with similarity to ubiquitin at the amino terminus and a RINGfingermotif at the carboxyl terminus. Parkinson’s disease is the second most commonneurodegenerative disease following Alzheimer’s. The characteristic movement disorderresults from dysfunction and neuron loss in the substantia nigra. Deletions in Parkinhave been associated with autosomal-recessive juvenile parkinsonism.Product Description: Paxillin is a focal adhesion protein and a substrate for severaltyrosine kinases such as SRC, FAK, and p210BRC/ABL. The tyrosine phosphorylation ofpaxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightlywith FAK and CRK through its SH2 domain. Although paxillin was initially discovered infibroblasts, its phosphorylation may also be important during neurite extension duringdifferentiation.PAX-5 Rabbit MonoclonalPCNA Rabbit PolyclonalCatalog No. Size FormatClone: SP34Catalog No. Size FormatClone: N/AM3340 0.1ml ConcentrateM3342 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human PAX-5 protein.E2850 0.1ml ConcentrateE2852 0.5ml ConcentrateImmunogen: Recombinant human PCNAprotein.M3344 1.0ml ConcentrateApplication: IHC-PE2854 1.0ml ConcentrateApplication: IHC-P, WBM3341 7.0ml Ready-to-UseSpecies Reactivity: Human*E2851 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Small Intestine, TonsilCellular Localization: NucleusCellular Localization: NucleusControl Lysate: 293TMolecular Weight: 30 kDaHuman Tonsil stained withanti-PAX-5 antibody M334Human Tonsil stained withanti-PCNA antibody E285Product Description: PAX-5 is a B cell specific activator protein. In the early stages of Bcell development, PAX-5 influences the expression of several B cell specific genes, suchas CD19 and CD20. PAX-5 is expressed primarily in pro-, pre-, and mature B cells, but notin plasma cells. PAX-5 mRNA is transiently detected in the mesencephalon and spinalcord during embryogenesis and then shifts to the fetal liver. This indicates that PAX-5 isimportant in B cell and neuronal development.Product Description: Expression of proliferating cell nuclear antigen (PCNA) or cyclinor polymerase delta auxiliary protein is elevated in the nucleus during late G1 phaseimmediately before the onset of DNA synthesis, becoming maximal during S-phase anddeclining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNApolymerase-delta to play a fundamental role in the initiation of cell proliferation.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 83

PD-1/CD279 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18660 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearE18662 0.5ml Concentrate N-terminus of human CD279 protein.E18664 1.0ml Concentrate Application: IHC-PE18661 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembranePDGFR alpha Rabbit PolyclonalCatalog No. Size FormatE2690 0.1ml ConcentrateE2692 0.5ml ConcentrateE2694 1.0ml ConcentrateE2691 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human PDGFR alpha.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, Ovarian Carcinoma, SkinCellular Localization: Cytoplasm, MembraneHuman Tonsil stained withanti-PD-1/CD279 antibody E1866Human Ovarian Carcinoma stained withanti-PDGFR alpha antibody E269Product Description: CD279, also known as Programmed Cell Death 1 (PD-1), is animmunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8thymocytes, and on activated T and B cells. It is thought to be involved in lymphocyteclonal selection and peripheral tolerance. The PD-1 ligands, PDL1 (also known as B7H1)and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.Product Description: PDGFR (Platelet Derived Growth Factor Receptor) exhibits twodifferent isoforms, alpha and beta, coded by two different genes. PDGF alpha receptorsare expressed in oligodendrocyte progenitor cells and PDGF beta receptors areexpressed on neurons. This antibody is specific for PDGFR alpha and does not react withPDGFR beta.PD-1/CD279 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18630 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE18632 0.5ml Concentrate region of human PD-1/CD279 protein.E18634 1.0ml Concentrate Application: IHC-PE18631 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lymph Node, TonsilCellular Localization: MembranePDGFR beta Rabbit PolyclonalCatalog No. Size FormatE2730 0.1ml ConcentrateE2732 0.5ml ConcentrateE2734 1.0ml ConcentrateE2731 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human PDGFR beta.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Kidney, Ovarian Carcinoma, SkinCellular Localization: Cytoplasm, MembraneHuman Lymph Node stained withanti-PD-1/CD279 antibody E1863Human Ovarian Carcinoma stained withanti-PDGFR beta antibody E273Product Description: CD279, also known as Programmed Cell Death 1 (PD-1), is animmunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8thymocytes, and on activated T and B cells. It is thought to be involved in lymphocyteclonal selection and peripheral tolerance. The PD-1 ligands, PDL1 (also known as B7H1)and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.Product Description: PDGFR (Platelet Derived Growth Factor Receptor) exhibits twodifferent isoforms, alpha and beta, coded by two different genes. PDGF alpha receptorsare expressed in oligodendrocyte progenitor cells and PDGF beta receptors areexpressed on neurons. This antibody is specific for PDGFR beta and does not react withPDGFR alpha.PDCD6 Rabbit PolyclonalPDK-1 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE17690 0.1ml ConcentrateE17692 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human PDCD6 protein.E18410 0.1ml ConcentrateE18412 0.5ml ConcentrateImmunogen: Synthetic peptide derived from thehuman PDK-1.E17694 1.0ml ConcentrateApplication: IHC-PE18414 1.0ml ConcentrateApplication: IHC-PE17691 7.0ml Ready-to-UseSpecies Reactivity: Human*E18411 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Lung CarcinomaControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneCellular Localization: CytoplasmHuman Lung Carcinoma stained withanti-PDCD6 antibody E1769Human Breast Carcinoma stained withanti-PDK-1 antibody E1841Product Description: This gene encodes a calcium-binding protein belonging to thepenta-EF-hand protein family. Calcium binding is important for homodimerization andfor conformational changes required for binding to other protein partners. This geneproduct participates in T cell receptor-, Fas-, and glucocorticoid-induced programmedcell death. In mice deficient for this gene product, however, apoptosis was not blockedsuggesting this gene product is functionally redundant.Product Description: Phosphoinositide-dependent protein kinase 1 (PDK1) plays acentral role in many signal transduction pathways, including the activation of Akt andPKC isoenzyme p70S6 kinase and RSK. Through its effects on these kinases, PDK1 isinvolved in the regulation of a wide variety of processes, including cell proliferation,differentiation and apoptosis.84Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesPDS-1/Securin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18530 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE18532 0.5ml Concentrate region of human PDS-1 protein.E18534 1.0ml Concentrate Application: IHC-PE18531 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Rectal CarcinomaCellular Localization: Cytoplasm, NucleusPhosphotyrosine Mouse MonoclonalCatalog No. Size Format Clone: SPM102E3080 0.1ml Concentrate Immunogen: Phosphotyrosine conjugated toE3082 0.5ml Concentrate protein carriers.E3084 1.0ml Concentrate Application: IHC-PE3081 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Mouse IgG1Control Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneHuman Rectal Carcinoma stained withanti-PDS-1/Securin antibody E1853Human Breast Carcinoma stained withanti-Phosphotyrosine antibody E308Product Description: Securin is an anaphase inhibitor and plays an important rolein DNA damage and spindle check point pathways. It is targeted for degradation byubiquitination mediated by a ubiquitin ligase - anaphase promoting complex (APC).Securin is highly expressed in various human tumors, including pituitary, adrenal,kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breasttumors, endometrial tumors, and ovarian tumors.Product Description: Protein phosphorylation is a fundamental event in the regulationof a large number of intracellular processes. This antibody is specific to phosphotyrosineand shows no cross-reaction with other phosphoamino acids.Peripheral Myelin Protein (PMP-22) Rabbit PolyclonalCatalog No. Size FormatE3520 0.1ml ConcentrateE3522 0.5ml ConcentrateE3524 1.0ml ConcentrateE3521 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from near C-terminus ofhuman PMP-22.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmPI3K p110beta Rabbit PolyclonalCatalog No. Size FormatE18210 0.1ml ConcentrateE18212 0.5ml ConcentrateE18214 1.0ml ConcentrateE18211 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human PI3k p110beta.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: CytoplasmHuman Pancreas stained withanti-Peripheral Myelin Protein (PMP-22) antibody E352Human Breast Carcinoma stained withanti-PI3K p110beta antibody E1821Product Description: PMP-22 (Peripheral Myelin Protein) is a glycoprotein expressed inthe compact myolin of the peripheral nervous system. In the peripheral nervous system,PMP-22 is produced by myelinating Schwann cells and is coexpressed with the genes formyelin basic protein (MBP) and protein P0 during nerve development and regeneration.Alterations in the level of this protein cause several genetic human diseases.Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring ofphosphatidylinositol. The enzyme comprises a 110kDa catalytic subunit and a regulatorysubunit of either 85, 55, or 50kDa. The loss of p110 beta decreases tumorigenesis in amouse cancer model with lipid phosphatase PTEN deficiency. This antibody is specific toPI3K p110beta.Phospho-Ser/Thr/Tyr Mouse MonoclonalCatalog No. Size FormatE3070 0.1ml ConcentrateE3072 0.5ml ConcentrateE3074 1.0ml ConcentrateN/A N/A N/AClone: SPM101Immunogen: Phosphoserine, phosphothreonine, andphosphotyrosine conjugated to protein carriers.Application: WBSpecies Reactivity: Human*Isotype: Mouse IgG1Control Tissue: N/ACellular Localization: N/AControl Lysate: JurkatMolecular Weight: 20 KDaPI3K p85 Rabbit MonoclonalCatalog No. Size FormatM3620 0.1ml ConcentrateM3622 0.5ml ConcentrateM3624 1.0ml ConcentrateM3621 7.0ml Ready-to-UseClone: SP62Immunogen: Synthetic peptide from N-terminus ofhuman PI3K p85.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmN/A E307Human Placenta stained withanti-PI3K p85 antibody M362Product Description: This antibody recognizes serine, threonine, and tyrosinephosphorylated proteins as determined by ELISA using phosphorylated peptides.Reactivity has also been shown with phosphorylated proteins in western blots.Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring ofphosphatidylinositol at the 3-prime position. The enzyme comprises a 110kDa catalyticsubunit and a regulatory subunit of either 85, 55, or 50kDa. This antibody is specific toPI3K p85 which is a 85kDa regulatory subunit.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 85

PI3K p85 Rabbit PolyclonalCatalog No. Size FormatE18200 0.1ml ConcentrateE18202 0.5ml ConcentrateE18204 1.0ml ConcentrateE18201 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human PI3K p85.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, PlacentaCellular Localization: CytoplasmPlacental Lactogen (hPL) Rabbit PolyclonalCatalog No. Size FormatE2980 0.1ml ConcentrateE2982 0.5ml ConcentrateE2984 1.0ml ConcentrateE2981 7.0ml Ready-to-UseClone: N/AImmunogen: Purified human placentallactogen.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmControl Lysate: Human PlacentaMolecular Weight: 21 kDaHuman Breast Carcinoma stained withanti-PI3K p85 antibody E1820Human Placenta stained withanti-Placental Lactogen (hPL) antibody E298Product Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring ofphosphatidylinositol at the 3-prime position. The enzyme comprises a 110kDa catalyticsubunit and a regulatory subunit of either 85, 55, or 50kDa. This antibody is specific toPI3K p85 which is a 85kDa regulatory subunit.Product Description: Human placental lactogen (hPL) is known to originate in thesyncytiotrophoblasts. Ultrastructural localization indicates that placental lactogenis stored in small morphologically distinct cytoplasmic granules within these cellsand has a different secretory mechanism from other hormonal peptides such ashuman chorionic gonadotropin. The level of expression of placental lactogen throughpregnancy increases gradually to term.PIM-1 Rabbit PolyclonalPLAP Rabbit MonoclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: SP15E18710 0.1ml ConcentrateE18712 0.5ml ConcentrateE18714 1.0ml ConcentrateE18711 7.0ml Ready-to-UseImmunogen: Synthetic peptide derived from internalregion of human PIM-1 protein.Application: IHC-PSpecies Reactivity: Human*M3150 0.1ml ConcentrateM3152 0.5ml ConcentrateM3154 1.0ml ConcentrateM3151 7.0ml Ready-to-UseImmunogen: Recombinant protein encoding humanplacental alkaline phosphatase.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Bone MarrowControl Tissue: Placenta, SeminomaCellular Localization: Cytoplasm, Membrane, NucleusCellular Localization: Cytoplasm, MembraneHuman Bone Marrow stained withanti-PIM-1 antibody E1871Human Seminoma stained withanti-PLAP antibody M315Product Description: PIM-1 is a member of the serine/threonine kinase PIM oncogenefamily. PIM-1 has been implicated in lymphomagenesis, cell proliferation, apoptosis,differentiation and tumourigenesis. The PIM-1 protein kinase is upregulated in prostatecancer.Product Description: This antibody reacts with a membrane-bound isoenzyme ofplacental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimesterof gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAPpositivesomatic cell tumors uniformly express epithelial membrane antigen (EMA).PIM-2 Rabbit PolyclonalPLAP Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE18800 0.1ml ConcentrateE18802 0.5ml ConcentrateE18804 1.0ml ConcentrateE18801 7.0ml Ready-to-UseImmunogen: Synthetic peptide derived from nearC-terminus of human PIM-2 protein.Application: IHC-PSpecies Reactivity: Human*E2040 0.1ml ConcentrateE2042 0.5ml ConcentrateE2044 1.0ml ConcentrateE2041 7.0ml Ready-to-UseImmunogen: Recombinant protein encoding humanplacental alkaline phosphatase.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: B Cell LymphomaControl Tissue: Placenta, SeminomaCellular Localization: Cytoplasm, NucleusCellular Localization: Cytoplasm, MembraneHuman B Cell Lymphoma stained withanti-PIM-2 antibody E1880Human Seminoma stained withanti-PLAP antibody E204Product Description: PIM-2 belongs to a family of cytoplasmic serine/threonineprotein kinases that consists of two other members, PIM-1 and PIM-3. Expression of thisprotein is regulated by hematopoietic cytokines. PIM-2 is most abundantly expressedin hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well asin testis, small intestine, and colon. PIM-2 is thought to be a mediator of p53 mediatedapoptosis inhibition by interleukin 3.Product Description: This antibody reacts with a membrane-bound isoenzyme ofplacental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimesterof gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAPpositivesomatic cell tumors uniformly express epithelial membrane antigen (EMA).86Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Poly ADP-Ribose Polymerase (PARP) Rabbit PolyclonalCatalog No. Size FormatE4220 0.1ml ConcentrateE4222 0.5ml ConcentrateE4224 1.0ml ConcentrateE4221 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human PARP protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusProgesterone Receptor (PR) Rabbit MonoclonalCatalog No. Size FormatM3420 0.1ml ConcentrateM3422 0.5ml ConcentrateM3424 1.0ml ConcentrateM3421 7.0ml Ready-to-UseClone: SP42Immunogen: Recombinant protein encoding aa 412-526 of human progesterone receptor.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusHuman Tonsil stained withanti-Poly ADP-Ribose Polymerase (PARP) antibody E422Human Breast Carcinoma stained withanti-Progesterone Receptor (PR) antibody M342Product Description: PARP uses nicotinamide adenine dinucleotide (oxidized form)NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear proteinacceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker ofprogrammed cell death and is mediated by the caspase CPP32 protein.Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. Ithas been proposed that expression of PR determination indicates a responsive estrogenreceptor (ER) pathway. A number of studies have shown that PR determination providessupplementary information to ER.Potassium Channel Kv3.2 Rabbit PolyclonalCatalog No. Size FormatE10800 0.1ml ConcentrateE10802 0.5ml ConcentrateE10804 1.0ml ConcentrateE10801 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from internal region ofrat potassium channel Kv3.2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmProgesterone Receptor (PR) Rabbit MonoclonalCatalog No. Size FormatM3020 0.1ml ConcentrateM3022 0.5ml ConcentrateM3024 1.0ml ConcentrateM3021 7.0ml Ready-to-UseClone: SP2Immunogen: Recombinant protein encoding aa412-526 of human progesterone receptor.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusHuman Brain stained withanti-Potassium Channel Kv3.2 antibody E1080Human Breast Carcinoma stained withanti-Progesterone Receptor (PR) antibody M302Product Description: Potassium channels contribute to maintaining cell volume,membrane potential, neuronal excitability and the secretion of transmitters, salt andhormones. Two families of potassium channels have been identified. One family includesthe inwardly rectifying potassium channels whereas, the other family includes: voltagesensing(KV); big conductance, calcium activated (BKca); and small conductance, calciumactivated (SK) potassium channels.Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. Ithas been proposed that expression of PR determination indicates a responsive estrogenreceptor (ER) pathway. A number of studies have shown that PR determination providessupplementary information to ER.Presenilin Rabbit PolyclonalCatalog No. Size FormatE3480 0.1ml ConcentrateE3482 0.5ml ConcentrateE3484 1.0ml ConcentrateE3481 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human presenilin 1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: Cytoplasm, MembraneProgesterone Receptor (PR) Rabbit PolyclonalCatalog No. Size FormatE2070 0.1ml ConcentrateE2072 0.5ml ConcentrateE2074 1.0ml ConcentrateE2071 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human PgR.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusHuman Brain stained withanti-Presenilin antibody E348Human Breast Carcinoma stained withanti-Progesterone Receptor (PR) antibody E207Product Description: In addition to β-amyloid precursor protein (β-APP), three othergene products have been implicated in Familial Alzheimer’s Disease (FAD): presenilin1 (PS-1), presenilin 2 (PS-2), and apolipoprotein E (apoE4). Mutations in the genesencoding β-APP, PS-1, and PS-2 are believed to contribute to presenile FAD.Product Description: The progesterone receptor (PR) is an estrogen-regulated protein. Ithas been proposed that expression of PR determination indicates a responsive estrogenreceptor (ER) pathway. A number of studies have shown that PR determination providessupplementary information to ER.88Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesProhibitin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17750 0.1ml Concentrate Immunogen: Synthetic peptide corresponding to nearE17752 0.5ml Concentrate C-terminus of human prohibitin protein.E17754 1.0ml Concentrate Application: IHC-PE17751 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: HeartCellular Localization: CytoplasmProstate Specific Antigen (PSA) Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2960 0.1ml Concentrate Immunogen: Purified human PSA.E2962 0.5ml Concentrate Application: IHC-PE2964 1.0ml ConcentrateSpecies Reactivity: Human*E2961 7.0ml Ready-to-UseIsotype: Rabbit IgGControl Tissue: Prostate, Prostate CarcinomaCellular Localization: CytoplasmHuman Heart stained withanti-Prohibitin antibody E1775Human Prostate stained withanti-Prostate Specific Antigen (PSA) antibody E296Product Description: Prohibitin is an evolutionarily conserved protein located in theinner membrane of mitochondria. Prohibitin appears to be a very reliable marker ofmitochondria. Prohibitin shows antiproliferative activity and has been proposed to playa role in normal cell cycle regulation, replicative senescence, cellular immortalization,and tumor suppression.Product Description: PSA is a chymotrypsin-like serine protease (kallikrein family)exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSAcan be detected in the sera of patients with prostatic carcinoma. It is predominantlycomplexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT).A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benignprostate hyperplasia.Prolactin Rabbit PolyclonalCatalog No. Size FormatE17490 0.1ml ConcentrateE17492 0.5ml ConcentrateE17494 1.0ml ConcentrateE17491 7.0ml Ready-to-UseClone: N/AImmunogen: Purified human prolactin.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PituitaryCellular Localization: CytoplasmProstate Stem Cell Antigen (PSCA) Rabbit PolyclonalCatalog No. Size FormatE3290 0.1ml ConcentrateE3292 0.5ml ConcentrateE3294 1.0ml ConcentrateE3291 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromextracellular region of human PSCA protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate, Prostate CarcinomaCellular Localization: Cytoplasm, MembraneHuman Pituitary stained withanti-Prolactin antibody E1749Human Prostate Carcinoma stained withanti-Prostate Stem Cell Antigen (PSCA) antibody E329Product Description: Prolactin is a pituitary hormone involved in the stimulation of milkproduction, salt and water regulation, growth, development and reproduction. Prolactinproducing cells make up approximately 20% of the pituitary.Product Description: PSCA (Prostate Stem Cell antigen) is a cell surface antigen, whichis overexpressed in approximately 40% of primary prostate cancers and in as many as100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell andpancreatic carcinomas.Prostate Apoptosis Response 4 (PAR-4) Rabbit PolyclonalCatalog No. Size FormatE3420 0.1ml ConcentrateE3422 0.5ml ConcentrateE3424 1.0ml ConcentrateE3421 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human prostate apoptosis response 4protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: HeartCellular Localization: CytoplasmProtein Gene Protein 9.5 (PGP9.5) Rabbit PolyclonalCatalog No. Size FormatE3340 0.1ml ConcentrateE3342 0.5ml ConcentrateE3344 1.0ml ConcentrateE3341 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant full lengthhuman protein gene product 9.5 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Pancreas, Small IntestineCellular Localization: Cytoplasm, NucleusControl Lysate: Human BrainMolecular Weight: 27 kDaHuman Heart stained withanti-Prostate Apoptosis Response 4 (PAR-4) antibody E342Human Pancreas stained withanti-Protein Gene Protein 9.5 (PGP9.5) antibody E334Product Description: Prostate apoptosis response-4 (PAR-4) is a 38kDa proteinoriginally identified as the product of a gene that is upregulated in prostate tumor cellsundergoing apoptosis. It is a leucine zipper and death domain-containing protein whoselevels increase in neurons undergoing apoptosis as a result of trophic factor withdrawalor exposure to oxidative and metabolic insults. PAR-4 levels are increased in the spinalcord specimens from patients with neuronal degeneration.Product Description: Protein gene product (PGP9.5) is a neuron specific protein distinctfrom neuron specific enolase. PGP9.5 is present in neurons, nerve fibers, neuroendocrinecells, renal tubules, spermatogonia, Leydig cells of the testis, ova and some cells of boththe pregnant and non-pregnant corpus luteum.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 89

PSMA Rabbit MonoclonalCatalog No. Size FormatM3290 0.1ml ConcentrateM3292 0.5ml ConcentrateM3294 1.0ml ConcentrateM3291 7.0ml Ready-to-UseClone: SP29Immunogen: Synthetic peptide derived fromC-terminus of human PSMA.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: MembraneRAD51 Rabbit PolyclonalCatalog No. Size FormatE19100 0.1ml ConcentrateE19102 0.5ml ConcentrateE19104 1.0ml ConcentrateE19101 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human RAD51 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilCellular Localization: NucleusHuman Prostate Carcinoma stained withanti-PSMA antibody M329Human Breast Carcinoma stained withanti-RAD51 antibody E1910Product Description: Prostate-specific membrane antigen (PMSA) is a type IItransmembrane glycoprotein belonging to the M28 peptidase family. Three functionallydistinct proteins are encoded including folylpoly-gamma-glutamate carboxypeptidasein the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, andprostate-specific membrane antigen in the prostate. PSMA is involved in prostate tumorprogression.Product Description: RAD51 is a member of the RAD51 protein family involved in repairof DNA double strand breaks. The protein is highly conserved in most eukaryotes fromyeast to humans. It can interact with the ssDNA-binding protein RPA and Rad52, and isthought to play roles in homologous pairing and strand transfer of DNA. The interactionsbetween Rad51 with BRCA1 and BRCA2 may be important for the cellular response toDNA damage. RAD51 is overexpressed in a variety of tumors.PSMA Rabbit PolyclonalCatalog No. Size FormatE17330 0.1ml ConcentrateE17332 0.5ml ConcentrateE17334 1.0ml ConcentrateE17331 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human PSMA.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: MembraneRAN Rabbit PolyclonalCatalog No. Size FormatE12460 0.1ml ConcentrateE12462 0.5ml ConcentrateE12464 1.0ml ConcentrateE12461 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa197-216 of human RAN protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BreastCellular Localization: NucleusHuman Prostate Carcinoma stained withanti-PSMA antibody E1733Human Breast stained withanti-RAN antibody E1246Product Description: Prostate-specific membrane antigen (PMSA) is a type IItransmembrane glycoprotein belonging to the M28 peptidase family. Three functionallydistinct proteins are encoded including folylpoly-gamma-glutamate carboxypeptidasein the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, andprostate-specific membrane antigen in the prostate. PSMA is involved in prostate tumorprogression.Product Description: The small Ras-related protein Ran, also called TC4, is a 24kDa,nuclear localized GTPase implicated in a diverse array of cellular processes includingDNA replication, entry into and exit from mitosis and the transport of RNA and proteinsthrough the nuclear pore complex.RACK-1 Rabbit PolyclonalCatalog No. Size FormatE18910 0.1ml ConcentrateE18912 0.5ml ConcentrateE18914 1.0ml ConcentrateE18911 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human RACK-1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Prostate CarcinomaCellular Localization: CytoplasmRCAS1 Rabbit PolyclonalCatalog No. Size FormatE16370 0.1ml ConcentrateE16372 0.5ml ConcentrateE16374 1.0ml ConcentrateE16371 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human RCAS1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Prostate Carcinoma stained withanti-RACK-1 antibody E1891Human Breast Carcinoma stained withanti-RCAS1 antibody E1637Product Description: RACK-1 (receptor for activated C kinase 1) was identified throughits binding to various PKC isoforms. Its main function is to recruit PKC and various otherproteins to specific locations to form multiprotein complexes, mediating various signalpathways.Product Description: RCAS-1 (Receptor binding cancer antigen expressed on SiSo cells)is a type III transmembrane protein mostly in the Golgi. It induces apoptosis of naturalkiller (NK)/T cells through activation of interleukin-1-beta converting enzyme (ICE)-likeproteases. RCAS-1 is abundantly expressed in prostate cancer cells when compared tonormal epithelial cells.90Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesRCC1 Rabbit PolyclonalCatalog No. Size FormatE13440 0.1ml ConcentrateE13442 0.5ml ConcentrateE13444 1.0ml ConcentrateE13441 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human RCC1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusRetinoic X Receptor gamma Rabbit PolyclonalCatalog No. Size FormatE4330 0.1ml ConcentrateE4332 0.5ml ConcentrateE4334 1.0ml ConcentrateE4331 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom N-terminus of mouse RXR gamma.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, Prostate CarcinomaCellular Localization: NucleusControl Lysate: NIH3T3Molecular Weight: 60 kDaHuman Colon Carcinoma stained withanti-RCC1 antibody E1344Human Prostate Carcinoma stained withanti-Retinoic X Receptor gamma antibody E433Product Description: Regulator of Chromosome Condensation 1 (RCC-1) binds tochromatin and participates in the regulation of chromosome condensation in the Sphase. RCC-1 interacts with GTP-binding nuclear protein Ran and catalyzes guaninenucleotide exchange. Both RCC-1 and Ran are components of an intrinsic checkpointcontrol that prevents the premature initiation of mitosis.Product Description: Retinoids are metabolites of vitamin A and represent importantsignaling molecules during vertebrate development and tissue differentiation. Twofamilies of retinoid receptors have been identified: retinoic acid receptors (RAR-α, RAR-βand RAR-γ); and retinoid X receptors (RXR-α, RXR-β and RXR-γ). RXRs are activated by9-cis-retinoic acid, a stereo- and photoisomer of all-trans-RA expressed in vivo in bothliver and kidney.Retinoblastoma (Rb) Rabbit PolyclonalCatalog No. Size FormatE4310 0.1ml ConcentrateE4312 0.5ml ConcentrateE4314 1.0ml ConcentrateE4311 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human retinoblastoma.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Colon CarcinomaCellular Localization: NucleusRHO Rabbit PolyclonalCatalog No. Size FormatE17850 0.1ml ConcentrateE17852 0.5ml ConcentrateE17854 1.0ml ConcentrateN/A N/A N/AClone: N/AImmunogen: Synthetic peptidecorresponding to human Rho protein.Application: WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: N/ACellular Localization: N/AControl Lysate: MCF7Molecular Weight: 21 kDaHuman Colon Carcinoma stained withanti-Retinoblastoma (Rb) antibody E431N/A E1785Product Description: Rb is a tumor suppressor nuclear phosphoprotein capable ofbinding to DNA. It is phosphorylated on serine and threonine, but not on tyrosineresidues. It forms a complex with SV40 large T antigen, adenovirus E1A, and humanpapilloma virus-16 E. Rb protein may act by regulating transcription and loss of itsfunction leads to uncontrolled cell growth.Product Description: The RHO proteins are members of the Ras superfamily of smallGTP-binding proteins. The RHO proteins play an important role in the organization ofactin cytoskeleton, cell adhesion, cell motility and smooth muscle contraction. The RHOproteins are also involved in the signal transduction pathways mediated by the actincytoskeleton and the activation of the stress activated MAP kinase cascades.Retinoic Acid Receptor beta Rabbit PolyclonalCatalog No. Size FormatE4370 0.1ml ConcentrateE4372 0.5ml ConcentrateE4374 1.0ml ConcentrateE4371 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human RAR beta.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Heart, TestisCellular Localization: NucleusROC1 Rabbit PolyclonalCatalog No. Size FormatE4190 0.1ml ConcentrateE4192 0.5ml ConcentrateE4194 1.0ml ConcentrateE4191 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human ROC1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusHuman Testis stained withanti-Retinoic Acid Receptor beta antibody E437Human Tonsil stained withanti-ROC1 antibody E419Product Description: Retinoids are metabolites of vitamin A and represent importantsignaling molecules during vertebrate development and tissue differentiation. Twofamilies of retinoid receptors have been identified: retinoic acid receptors (RARs-α, -βand -γ); and retinoid X receptors (RXR-α, -β and -γ). RXRs have a high affinity for all-transretinoic acids and belong to the same class of nuclear transcription factors such asthyroid hormone receptors and vitamin D3 receptor.Product Description: ROC1 and ROC2 are the two highly conserved RING finger proteinsthat are homologous to APC11, a subunit of the anaphase-promoting complex (APC).ROC1 and ROC2 commonly interact with all cullins. ROC1 and APC11 immunocomplexescan catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 is thought to play a unique role in the ubiquitination reactionby heterodimerizing with cullin1 to catalyze ubiquitin polymerization.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 91

RRM1 Rabbit PolyclonalCatalog No. Size FormatE18040 0.1ml ConcentrateE18042 0.5ml ConcentrateE18044 1.0ml ConcentrateE18041 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman RRM1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: Cytoplasm, NucleusS6 Ribosomal Rabbit PolyclonalCatalog No. Size FormatE17780 0.1ml ConcentrateE17782 0.5ml ConcentrateE17784 1.0ml ConcentrateE17781 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom internal region of human S6 ribosomal.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: NIH3T3Molecular Weight: 32 kDaHuman Placenta stained withanti-RRMI antibody E1804Human Breast Carcinoma stained withanti-S6 Ribosomal antibody E1778Product Description: RRM1 (Ribonucleotide reductase M1 polypeptide) is one oftwo non-identical subunits that constitute ribonucleoside-diphosphate reductase,an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from thecorresponding ribonucleotides. It provides the precursors necessary for DNA synthesis.RRM1 is involved in carcinogenesis and tumor progression.Product Description: 40S ribosomal protein S6 (also known as RPS6) is a 31kDasubstrate of p70 S6 kinase (p70S6K) and a major component of translational machineryinvolved in protein synthesis, cell growth, proliferation, and metabolism. RPS6undergoes phosphorylation on multiple serines in the carboxyl terminal region in theorder 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates proteinsynthesis that mediates progression through the cell cycle.S100 Rabbit PolyclonalCatalog No. Size FormatE2140 0.1ml ConcentrateE2142 0.5ml ConcentrateE2144 1.0ml ConcentrateE2141 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding humanS100 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Melanoma, SchwannomaCellular Localization: CytoplasmHuman Melanoma stained withanti-S100 antibody E214Product Description: S100 belongs to the family of calcium binding proteins such ascalmodulin and troponin C. S100A is composed of an alpha and beta chain whereasS100B is composed of two beta chains. The antibody to S100 stains schwannomas,ependymomas, astrogliomas, almost all benign and malignant melanomas and theirmetastases. S100 protein is also expressed in the antigen presenting cells such as theLangerhans cells in skin and interdigitating reticulum cells.S6 Ribosomal (Paired 235/236) Rabbit PolyclonalCatalog No. Size FormatE18080 0.1ml ConcentrateE18082 0.5ml ConcentrateE18084 1.0ml ConcentrateE18081 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom the region of human S6 ribosomal thatcontains unphosphorylated serines 235and 236.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmControl Lysate: 293TMolecular Weight: 32 kDaHuman Breast Carcinoma stained withanti-S6 Ribosomal (Paired 235/236) antibody E1808Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase(p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. This antibody detects non-phosphorylated S6 ribosomal at 235/236 serine residue.S100A4 Rabbit PolyclonalCatalog No. Size FormatE4440 0.1ml ConcentrateE4442 0.5ml ConcentrateE4444 1.0ml ConcentrateE4441 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant proteinencoding full length human S100A4.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Melanoma, TonsilCellular Localization: CytoplasmControl Lysate: Human PlacentaMolecular Weight: 12 kDaS6 Ribosomal (Paired 240/244) Rabbit PolyclonalCatalog No. Size FormatE17770 0.1ml ConcentrateE17772 0.5ml ConcentrateE17774 1.0ml ConcentrateE17771 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from theregion of human S6 ribosomal that contains nonphosphorylatedserines 240 and 244.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Tonsil stained withanti-S100A4 antibody E444Human Breast Carcinoma stained withanti-S6 Ribosomal (Paired 240/244) antibody E1777Product Description: S100 belongs to the family of calcium binding proteins such ascalmodulin and troponin C. S100A is composed of an alpha and beta chain whereasS100B is composed of two beta chains. S100 protein is also expressed in the antigenpresenting cells such as the Langerhans cells in skin and interdigitating reticulum cells inthe paracortex of lymph node.Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase(p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detectsnon-phosphorylated S6 ribosomal at 240/244 serine residue.92Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesS6 Ribosomal (Phospho S235/236) Rabbit MonoclonalCatalog No. Size FormatM3500 0.1ml ConcentrateM3502 0.5ml ConcentrateM3504 1.0ml ConcentrateM3501 7.0ml Ready-to-UseClone: SP50Immunogen: Synthetic peptide derived from the regionof human S6 ribosomal that contains phosphorylatedserines 235 and 236.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmS6 Ribosomal (Phospho S240/244) Rabbit PolyclonalCatalog No. Size FormatE17760 0.1ml ConcentrateE17762 0.5ml ConcentrateE17764 1.0ml ConcentrateE17761 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from the regionof human S6 ribosomal that contains phosphorylatedserines 240 and 244.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-S6 Ribosomal (Phospho S235/236) antibody M350Human Breast Carcinoma stained withanti-S6 Ribosomal (Phospho S240/244) antibody E1776Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase(p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detectsS6 ribosomal only when it is phosphorylated at 235/236 serine residue.Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase(p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detectsS6 Ribosomal only when it is phosphorylated at 240/244 serine residue.S6 Ribosomal (Phospho S235/236) Rabbit PolyclonalCatalog No. Size FormatE18070 0.1ml ConcentrateE18072 0.5ml ConcentrateE18074 1.0ml ConcentrateE18071 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from the regionof human S6 ribosomal that contains phosphorylatedserines 235 and 236.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmSHIP-1 Rabbit PolyclonalCatalog No. Size FormatE14060 0.1ml ConcentrateE14062 0.5ml ConcentrateE14064 1.0ml ConcentrateE14061 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human SHIP-1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: MembraneHuman Breast Carcinoma stained withanti-S6 Ribosomal (Phospho S235/236) antibody E1807Human Tonsil stained withanti-SHIP-1 antibody E1406Product Description: 40S ribosomal protein S6 (RPS6) is a substrate of p70 S6 kinase(p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. Hyperphosphorylation of RPS6 stimulates protein synthesis. This antibody detectsS6 ribosomal only when it is phosphorylated at 235/236 serine residue.Product Description: SHIP-1 p145 (SH2-containing-inositol phosphatase) containstwo phosphotyrosine-binding domains (PTB), a unique amino terminal SH2 domain,and two highly conserved motifs found among inositol phosphate 5-phosphatases.SHIP-1 displays both phosphatidylinositol 3,4,5-triphosphate and inositol 1, 3, 4, 5-tetrakisphosphate polyphosphate 5-phosphatase activity. Evidence suggests thatSHIP-1 may modulate Ras signaling in addition to inositol signaling pathways.S6 Ribosomal (Phospho S240/244) Rabbit MonoclonalCatalog No. Size FormatM3450 0.1ml ConcentrateM3452 0.5ml ConcentrateM3454 1.0ml ConcentrateM3451 7.0ml Ready-to-UseClone: SP45Immunogen: Synthetic peptide derived from the regionof human S6 ribosomal that contains phosphorylatedserines 240 and 244.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmSH-PTP2 Rabbit PolyclonalCatalog No. Size FormatE12440 0.1ml ConcentrateE12442 0.5ml ConcentrateE12444 1.0ml ConcentrateE12441 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding to aa576-593 of human SH-PTP2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bone MarrowCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-S6 Ribosomal (Phospho S240/244) antibody M345Human Bone Marrow stained withanti-SH-PTP2 antibody E1244Product Description: 40S ribosomal protein S6 (RPS6) is a 31kDa substrate of p70 S6kinase (p70S6K) and a major component of translational machinery involved in proteinsynthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylationon multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247. This antibody detects S6 ribosomal only when it is phosphorylated at 240/244serine residue.Product Description: The steady state of protein tyrosyl phosphorylation in cells isregulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases(PTPs). SH-PTP1 is primarily expressed in hematopoietic cells and characterized by thepresence of two SH2 domains N-terminal to the PTP domain. SH-PTP2 works on thedownstream of various receptor and cytoplasmic protein tyrosine kinases to participatein the signal transduction from the cell surface to the nucleus.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 93

SKP1 p19 Rabbit PolyclonalCatalog No. Size FormatE14120 0.1ml ConcentrateE14122 0.5ml ConcentrateE14124 1.0ml ConcentrateE14121 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to C-terminus of humanSKP1 p19.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: NucleusControl Lysate: NIH3T3Molecular Weight: 19 kDaSLIT2 Rabbit PolyclonalCatalog No. Size FormatE19050 0.1ml ConcentrateE19052 0.5ml ConcentrateE19054 1.0ml ConcentrateE19051 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide near N-terminus ofhuman SLIT2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Fetal Kidney, Prostate CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-SKP1 p19 antibody E1412Human Prostate Carcinoma stained withanti-SLIT2 antibody E1905Product Description: SKP1 p19 and SKP2 p45 are two cyclin A-CDK2 complex bindingproteins participating in cell cycle regulation. Cyclin A accumulates prior to cyclin Bin the cell cycle, appears to be involved in control of S phase and has been shown toassociate with cyclin dependent kinase-2 (CDK2). Although the Skps (S phase kinaseassociatedproteins) associate with the active cyclin A-Cdk2 complex, they do not exhibitany regulatory effects on the complex.Product Description: Slit homolog 2 proteins (SLIT2) are ligands for the Roundabout(Robo) receptors and act as molecule guidance cues in cell migration. Slit1 and Slit2 areessential for midline guidance in the forebrain by acting as repulsive signals preventinginappropriate midline crossing by axons projecting from the olfactory bulb. Slit2protein is expressed in fetal lung and kidney, and adult spinal cord. SLIT2 is a target forrepression by EZH2 and is up-regulated in prostate cancer.SKP2 p45 Rabbit PolyclonalCatalog No. Size FormatE13880 0.1ml ConcentrateE13882 0.5ml ConcentrateE13884 1.0ml ConcentrateE13881 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa221-425 of human SKP2 alpha.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ProstateCellular Localization: Cytoplasm, NucleusSMA Rabbit PolyclonalCatalog No. Size FormatE19220 0.1ml ConcentrateE19222 0.5ml ConcentrateE19224 1.0ml ConcentrateE19221 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide near the N-terminus ofhuman smooth muscle actin-alpha.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, Small IntestineCellular Localization: CytoplasmHuman Prostate stained withanti-SKP2 p45 antibody E1388Human Small Intestine stained withanti-SMA antibody E1922Product Description: SKP2 (S-phase Kinase-associated Protein 2) belongs to the familyof F-box proteins that interact with the cyclin A-CDK2 complex. SKP2 is essential forthe G1-S transition in both transformed cells and diploid fibroblasts. SKP2 is widelyexpressed in normal and cancer tissues such as tonsil, placenta and cancers from colon,prostate, pancreas, skin, lung, breast, ovarian, and endometrium. SKP2 plays a role inoncogenesis, particularly in lymphomas and breast cancers.Product Description: Smooth muscle actin-alpha is a cytoskeleton protein in smoothmuscle cells and their derived tumors such as leiomyoma and leiomyosarcoma. It is alsoexpressed in myoepithelial cells of the breast and salivary gland, but not in fibroblasts,striated muscle, and myocardium.SLIT2 Rabbit MonoclonalCatalog No. Size FormatM4220 0.1ml ConcentrateM4222 0.5ml ConcentrateM4224 1.0ml ConcentrateM4221 7.0ml Ready-to-UseClone: SP122Immunogen: Synthetic peptide corresponding toC-terminus of human SLIT2 protein.Application: IHC-PSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: Prostate AdenocarcinomaCellular Localization: CytoplasmSMAD3 Rabbit PolyclonalCatalog No. Size FormatE11250 0.1ml ConcentrateE11252 0.5ml ConcentrateE11254 1.0ml ConcentrateE11251 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human SMAD3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast, Breast CarcinomaCellular Localization: NucleusHuman Prostate Adenocarcinoma stained withanti-SLIT2 antibody M422Human Breast Carcinoma stained withanti-SMAD3 antibody E1125Product Description: SLIT2 are ligands for Robo receptors and play a vital role for axonalmigration/navigation during neural development. A number of cleavage productsthrough alternate splicing are reported in the literature for SLIT2 proteins. The C-terminalcleavage proteins are more diffusible than the larger N-terminal protein that is moretightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, andadult spinal cord.Product Description: SMAD proteins mediate signaling events leading to transcriptionalactivation initiated by members of the TGF-beta superfamily. Biological activitiesmediated by smads include cell growth and morphogenesis, development, andimmune response. A reduction in SMAD2/3 signaling enhances tumorigenesis, but alsosuppresses metastasis of breast cancer cell lines.94Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesSMRTe Rabbit PolyclonalSOCS-6 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE10990 0.1ml ConcentrateE10992 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding to aa994-1005 of human SMRTe protein.E16780 0.1ml ConcentrateE16782 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human SOCS-6 protein.E10994 1.0ml ConcentrateApplication: IHC-PE16784 1.0ml ConcentrateApplication: IHC-PE10991 7.0ml Ready-to-UseSpecies Reactivity: Human*E16781 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Brain, Lung CarcinomaControl Tissue: PlacentaCellular Localization: NucleusCellular Localization: CytoplasmHuman Brain stained withanti-SMRTe antibody E1099Human Placenta stained withanti-SOCS-6 antibody E1678Product Description: SMRT is 1495 amino acids and contains an 8 amino acid sequencethat is not present in SMRTe (SMRT-extended), which contains 2514 amino acids. SMRTecontains an N-terminal sequence spanning over 1,000 amino acids that is not presentin SMRT, but that shows significant similarity with N-CoR. SMRTe expression is regulatedduring cell cycle progression, suggesting a role for SMRTe in the regulation of cyclespecificgene expression in diverse signaling pathways.Product Description: Suppressor of Cytokine Signaling 6 (SOCS-6) interacts with theinsulin receptor (IR) when expressed in human hepatoma cells (HepG2) overexpressinghuman IR. SOCS-1 and SOCS-6 inhibit insulin-dependent activation of ERK1/2 andprotein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. SOCS-6proteins may be inhibitors of IR signaling and could mediate cytokine-induced insulinresistance and contribute to the pathogenesis of type II diabetes.SOCS-2 Rabbit PolyclonalCatalog No. Size FormatE16720 0.1ml ConcentrateE16722 0.5ml ConcentrateE16724 1.0ml ConcentrateE16721 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human SOCS-2 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: CytoplasmSodium Channel-pan Rabbit PolyclonalCatalog No. Size FormatE10290 0.1ml ConcentrateE10292 0.5ml ConcentrateE10294 1.0ml ConcentrateE10291 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofthe alpha subunit of rat type 1 voltage-gated sodiumchannel.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: CytoplasmHuman Pancreas stained withanti-SOCS-2 antibody E1672Human Brain stained withanti-Sodium Channel-pan antibody E1029Product Description: Suppressor of Cytokine Signaling (SOCS) includes eight membersof SOCS family (SOCS-1, 2, 3, 4, 5, 6, 7 and CIS). Structurally the SOCS proteins arecomposed of an N-terminal region of variable length and amino acid composition, acentral SH2 domain, and a previously unrecognized C-terminus motif called the SOCSbox. The SOCS-2 appear to form part of a classical negative feed back loop that regulatescytokine signal transduction.Product Description: Voltage-gated sodium channels (VGSC) are present in mostexcitable cells. In neuronal tissue, they are responsible for generating and propagatingaction potentials. Brain VGSCs are heteromers of αβ1β2 subunits. Of these, the α subunitforms the channel pore. This antibody recognizes the alpha subunit of type I voltagegatedsodium channel.SOCS-3 Rabbit PolyclonalCatalog No. Size FormatE16850 0.1ml ConcentrateE16852 0.5ml ConcentrateE16854 1.0ml ConcentrateE16851 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human SOCS-3 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Placenta, TestisCellular Localization: CytoplasmSonic Hedgehog Rabbit PolyclonalCatalog No. Size FormatE17920 0.1ml ConcentrateE17922 0.5ml ConcentrateE17924 1.0ml ConcentrateE17921 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human sonic hedgehog.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Fetal Brain, LungCellular Localization: Extracellular Matrix, MembraneHuman Placenta stained withanti-SOCS-3 antibody E1685Human Lung stained withanti-Sonic Hedgehog antibody E1792Product Description: Suppressor of Cytokine Signaling 3 (SOCS-3) is involved innegative regulation of cytokines that signal through the JAK/STAT pathway. It inhibitscytokine signal transduction by binding to tyrosine kinase receptors including gp130,LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. It suppresses fetal livererythropoiesis and regulates onset and maintenance of allergic responses mediated byT-helper type 2 cells.Product Description: Sonic Hedgehog (SHH) is expressed only during embryogenesisand is instrumental in patterning in the early embryo. It has been implicated as the keyinductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis,and the ventral somites. SHH binds to the patched (PTC) receptor, which functions inassociation with smoothened (SMO), to activate the transcription of target genes.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 95

SOX-2 Rabbit MonoclonalCatalog No. Size FormatM3760 0.1ml ConcentrateM3762 0.5ml ConcentrateM3764 1.0ml ConcentrateM3761 7.0ml Ready-to-UseClone: SP76Immunogen: Synthetic peptidecorresponding to N-terminus of humanSOX-2 protein.Application: IHC-P, WBSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: Newborn BrainCellular Localization: NucleusControl Lysate: MCF7Molecular Weight: 34 kDaSOX-9 Rabbit PolyclonalCatalog No. Size FormatE14040 0.1ml ConcentrateE14042 0.5ml ConcentrateE14044 1.0ml ConcentrateE14041 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa316-510 of human SOX-9 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TestisCellular Localization: NucleusHuman Newborn Brain stained withanti-SOX-2 antibody M376Human Testis stained withanti-SOX-9 antibody E1404Product Description: SOX-2 is also known as SRY related HMG BOX gene 2. It is atranscription factor that forms a trimeric complex with OCT4 on DNA and controls theexpression of a number of genes involved in embryonic development such as YES1,FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cellpluripotency. SOX-2 is highly expressed in glioma, lung cancer and stomach cancer.Product Description: SOX-9 is a member of the SOX family (Sry-type high mobilitygroup box) genes that was first identified on the basis of region with high homologyto that of SRY (Sex-determining region Y). SOX-9 is a transcription factor with a highmobility group DNA-binding domain in all prechondrocytic and chondrocytic cellsduring embryonic development. SOX-9 regulates expression of chondrocyte phenotyperelated genes.SOX-2 Rabbit PolyclonalCatalog No. Size FormatE18600 0.1ml ConcentrateE18602 0.5ml ConcentrateE18604 1.0ml ConcentrateE18601 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human SOX-2 proteinApplication: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Newborn Brain, SkinCellular Localization: NucleusSOX-9 Rabbit PolyclonalCatalog No. Size FormatE13470 0.1ml ConcentrateE13472 0.5ml ConcentrateE13474 1.0ml ConcentrateE13471 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein encoding aa 1-150of human SOX-9 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TestisCellular Localization: NucleusHuman Newborn Brain stained withanti-SOX-2 antibody E1860Human Testis stained withanti-SOX-9 antibody E1347Product Description: SOX-2 is also known as SRY related HMG BOX gene 2. It is atranscription factor that forms a trimeric complex with OCT4 on DNA and controls theexpression of a number of genes involved in embryonic development such as YES1,FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cellpluripotency. SOX-2 is highly expressed in glioma, lung cancer and stomach cancer.Product Description: SOX-9 is a member of the SOX family (Sry-type high mobilitygroup box) genes that was first identified on the basis of region with high homologyto that of SRY (Sex-determining region Y). SOX-9 is a transcription factor with a highmobility group DNA-binding domain in all prechondrocytic and chondrocytic cellsduring embryonic development. SOX-9 regulates expression of chondrocyte phenotyperelated genes.SOX-4 Rabbit PolyclonalCatalog No. Size FormatE16380 0.1ml ConcentrateE16382 0.5ml ConcentrateE16384 1.0ml ConcentrateE16381 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human SOX-4 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: NucleusSOX-11 Rabbit PolyclonalCatalog No. Size FormatE18760 0.1ml ConcentrateE18762 0.5ml ConcentrateE18764 1.0ml ConcentrateE18761 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human SOX-11 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Mantle Cell LymphomaCellular Localization: NucleusHuman Brain stained withanti-SOX-4 antibody E1638Human Mantle Cell Lymphoma stained withanti-SOX-11 antibody E1876Product Description: SOX-4 is a member of the SOX (SRY-related HMG-box) family oftranscription factors involved in the regulation of embryonic development and in thedetermination of the cell fate. SOX-4 binds with high affinity to the T cell enhancermotif 5’-AACAAAG-3’ motif. Sox-4 is expressed in lymphocytes (B and T) and it isrequired for B lymphocyte development. It is also expressed in breast, breast cancer,medulloblastoma, and prostate cancer.Product Description: The SOX (SRY-related HMG-box) family are transcription factorsinvolved in the regulation of embryonic development and in the determination of thecell fate. All SOX proteins have a single HMG box and bind linear DNA. SOX-1, SOX-2and SOX-3 show the closest homology to SRY. SOX-11 appears to be important in thedevelopment of nervous system and tissue modeling during development.96Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesSOX-14 Rabbit PolyclonalCatalog No. Size FormatE16820 0.1ml ConcentrateE16822 0.5ml ConcentrateE16824 1.0ml ConcentrateE16821 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human SOX-14 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Fetal BrainCellular Localization: NucleusStanniocalcin-2 Rabbit PolyclonalCatalog No. Size Format Clone: N/AE18840 0.1ml Concentrate Immunogen: Synthetic peptide derived from internalE18842 0.5ml Concentrate region of human stanniocalcin-2 protein.E18844 1.0ml Concentrate Application: IHC-PE18841 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Small IntestineCellular Localization: CytoplasmHuman Fetal Brain stained withanti-SOX-14 antibody E1682Human Small Intestine stained withanti-Stanniocalcin-2 antibody E1884Product Description: The SOX (SRY-box containing gene) gene family encodes a groupof transcription factors defined by the conserved high motility group (HMG) DNAbindingdomain. They are involved in the regulation of embryonic development andin the determination of cell fate (specifically neuronal). SOX-14 is most closely relatedto SOX factors 1, 2 and 3 and likely mirrors expression of these genes in the developingbrain and spinal cord.Product Description: Stanniocalcin-2 is a homodimeric glycoprotein that is expressedin a wide variety of tissues and may have autocrine or paracrine functions. The proteinmay play a role in the regulation of renal and intestinal calcium and phosphate transportand cell metabolism. In the ovary, it has been shown to be a paracrine hormone thatregulates granulosa cell function. Expression of this gene is induced by estrogen andaltered in some breast cancers.SP1 Rabbit PolyclonalSTAT3 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE12540 0.1ml ConcentrateE12542 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human SP1.E3690 0.1ml ConcentrateE3692 0.5ml ConcentrateImmunogen: Recombinant protein encoding aa 50-240of human Stat3.E12544 1.0ml ConcentrateApplication: IHC-PE3694 1.0ml ConcentrateApplication: IHC-PE12541 7.0ml Ready-to-UseSpecies Reactivity: Human*E3691 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Breast CarcinomaCellular Localization: NucleusCellular Localization: Cytoplasm, NucleusHuman Tonsil stained withanti-SP1 antibody E1254Human Breast Carcinoma stained withanti-STAT3 antibody E369Product Description: SP1 is a sequence-specific transcription factor that recognizesGGGGCGGGGC and closely related sequences, which are often referred to as GC boxes.SP1 belongs to a subgroup of transcription factors that are phosphorylated uponbinding to promoter sequences.Product Description: The STAT3 protein is a transcription factor expressed in mostcell types and is activated by multiple cytokines and growth factors including: IFN-a,IL-10, IL-6, IL-11, IL-12, IL-2, EGF etc. STAT3 binds to DNA as either a homodimer or as aheterodimer with STAT1. STAT3 interacts with the APRE (acute phase response element)and SIE (c-sis inducible element).ST13 Rabbit PolyclonalCatalog No. Size FormatE17870 0.1ml ConcentrateE17872 0.5ml ConcentrateE17874 1.0ml ConcentrateE17871 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tohuman ST13.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: NucleusSTAT5 alpha Rabbit PolyclonalCatalog No. Size FormatE3590 0.1ml ConcentrateE3592 0.5ml ConcentrateE3594 1.0ml ConcentrateE3591 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom C-terminus of human STAT5 alpha.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilCellular Localization: NucleusControl Lysate: K562Molecular Weight: 95 kDaHuman Colon Carcinoma stained withanti-ST13 antibody E1787Human Breast Carcinoma stained withanti-STAT5 alpha antibody E359Product Description: ST13 is a cofactor of heat shock protein 70 (HSP70). To date, alldata since the discovery of ST13 in 1993 until more recent studies in 2007 have provedthat ST13 is down regulated in tumors and it is proposed to be a tumor suppressor gene.Product Description: STAT5a protein is a transcription factor activated by hormoneand cytokines. Two highly related, but distinct STAT5 genes (STAT5a and STAT5b) wereidentified in mouse. STAT5a is critically involved in a variety of physiological functions,including reproduction, lactation, immune function and somatic growth.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 97

STAT6 Rabbit PolyclonalCatalog No. Size FormatE4290 0.1ml ConcentrateE4292 0.5ml ConcentrateE4294 1.0ml ConcentrateE4291 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human STAT6.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Placenta, TonsilCellular Localization: Cytoplasm, NucleusSurvivin Rabbit MonoclonalCatalog No. Size FormatM3790 0.1ml ConcentrateM3792 0.5ml ConcentrateM3794 1.0ml ConcentrateM3791 7.0ml Ready-to-UseClone: SP79Immunogen: Synthetic peptide corresponding toC-terminus of human survivin protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: CytoplasmHuman Placenta stained withanti-STAT6 antibody E429Human Stomach stained withanti-Survivin antibody M379Product Description: STAT6 protein is a transcription factor activated by cytokines,particularly interleukin-4 and IL13. STAT6 was found to be deficient in IL-4-mediatedfunctions including Th2 helper T-cell differentiation, expression of cell surfacemarkers, and T-cell proliferation. STAT6 mRNA has been detected in peripheral bloodlymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung andplacenta. STAT6 is critically involved in Th2 immune response.Product Description: Survivin is an inhibitor of apoptosis (IAP) protein. It is expressedin the G2/M phase of the cell cycle in a cycle-regulated manner. The overexpressionof survivin in cancer may overcome this apoptotic checkpoint and favor aberrantprogression of transformed cells through mitosis.Stathmin Rabbit MonoclonalCatalog No. Size Format Clone: SP49M3490 0.1ml Concentrate Immunogen: Synthetic peptide derivedM3492 0.5ml Concentrate from internal region of human stathmin.M3494 1.0ml Concentrate Application: IHC-P, WBM3491 7.0ml Ready-to-Use Species Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmControl Lysate: NIH3T3Molecular Weight: 18 kDaSurvivin Rabbit PolyclonalCatalog No. Size FormatE3770 0.1ml ConcentrateE3772 0.5ml ConcentrateE3774 1.0ml ConcentrateE3771 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human survivin.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: StomachCellular Localization: CytoplasmHuman Tonsil stained withanti-Stathmin antibody M349Human Stomach stained withanti-Survivin antibody E377Product Description: Stathmin is a ubiquitous cytosolic phosphoprotein proposedto function as an intracellular relay integrating regulatory signals of the cellularenvironment. The encoded protein is involved in the regulation of the microtubulefilament system by destabilizing microtubules. It prevents assembly and promotesdisassembly of microtubules. Alternatively spliced transcript variants encoding the sameprotein have been identified.Product Description: Survivin is an inhibitor of apoptosis (IAP) protein. It is expressedin the G2/M phase of the cell cycle in a cycle-regulated manner. The overexpressionof survivin in cancer may overcome this apoptotic checkpoint and favor aberrantprogression of transformed cells through mitosis.Stathmin Rabbit PolyclonalCatalog No. Size FormatE18060 0.1ml ConcentrateE18062 0.5ml ConcentrateE18064 1.0ml ConcentrateE18061 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom internal region of human stathmin.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast Carcinoma, TonsilCellular Localization: CytoplasmControl Lysate: NIH3T3Molecular Weight: 18 KDaSYK Rabbit PolyclonalCatalog No. Size FormatE4050 0.1ml ConcentrateE4052 0.5ml ConcentrateE4054 1.0ml ConcentrateE4051 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derivedfrom internal region of human SYK protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bone Marrow, LymphomaCellular Localization: CytoplasmControl Lysate: HepG2Molecular Weight: 72 KDaHuman Breast Carcinoma stained withanti-Stathmin antibody E1806Human Bone Marrow stained withanti-SYK antibody E405Product Description: Stathmin is a ubiquitous cytosolic phosphoprotein proposedto function as an intracellular relay integrating regulatory signals of the cellularenvironment. The encoded protein is involved in the regulation of the microtubulefilament system by destabilizing microtubules. It prevents assembly and promotesdisassembly of microtubules. Alternatively spliced transcript variants encoding the sameprotein have been identified.Product Description: SYK, a protein tyrosine kinase (PTK) is expressed in immature Tcells, all B cells, basophils, hepatocytes, platelets, and erythrocytes. It functions in someT cell receptor signaling processes in addition to B cell receptor and integrin-mediatedsignal transduction processes. SYM-/syk- mice exhibit defective phagocytosis bymacrophages.98Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesSynaptophysin Rabbit MonoclonalCatalog No. Size Format Clone: SP11M3110 0.1ml Concentrate Immunogen: Synthetic peptideM3112 0.5ml Concentrate corresponding to C-terminus of humanM3114 1.0ml Concentrate synaptophysin.M3111 7.0ml Ready-to-Use Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast NeuroendocrineTumor, Pancreas, PheochromcytomaCellular Localization: CytoplasmControl Lysate: Human BrainMolecular Weight: 38 kDaHuman Neuroendocrine Tumor stained withanti-Synaptophysin antibody M311Product Description: Synaptophysin is expressed in neuroendocrine cells ofhuman adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas,gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietalcells, neurons in the brain, spinal cord, and retina. It is also expressed in neuroendocrinetumors such as neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas,chromaffin, and non-chromaffin paragangliomas.Syntaxin 1A Rabbit PolyclonalCatalog No. Size FormatE17700 0.1ml ConcentrateE17702 0.5ml ConcentrateE17704 1.0ml ConcentrateE17701 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human syntaxin 1A protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: MembraneHuman Brain stained withanti-Syntaxin 1A antibody E1770Product Description: Syntaxin 1A (STX1A) is potentially involved in docking of synapticvesicles at presynaptic active zones and may play a critical role in neurotransmitterexocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular andmusculoskeletal abnormalities observed in Williams-Beuren syndrome (WBS), a raredevelopmental disorder. It is a contiguous gene deletion syndrome involving genesfrom chromosome band 7q11.23.Synaptophysin Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2170 0.1ml Concentrate Immunogen: Synthetic peptide of humanE2172 0.5ml Concentrate synaptophysin.E2174 1.0ml Concentrate Application: IHC-PE2171 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon, PancreasCellular Localization: CytoplasmSynuclein-alpha Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2680 0.1ml Concentrate Immunogen: Synthetic peptide corresponding to nearE2682 0.5ml Concentrate C-terminus of human synuclein-alpha.E2684 1.0ml Concentrate Application: IHC-PE2681 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain (Posterior Cingulated Gyrus)Cellular Localization: Cytoplasm, Presynaptic TerminalsHuman Pancreas stained withanti-Synaptophysin antibody E217Human Brain stained withanti-Synuclein-alpha antibody E268Product Description: Synaptophysin is expressed in neuroendocrine cells ofhuman adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas,gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietalcells, neurons in the brain, spinal cord, and retina. It is also expressed in neuroendocrinetumors such as neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas,chromaffin, and non-chromaffin paragangliomas.Product Description: Synucleins are small, soluble proteins expressed primarily in neuraltissue and in certain tumors. The family includes three known proteins: alpha-synuclein,beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins arefound primarily in brain tissue, where they are seen mainly in presynaptic terminals. Thisantibody is specific for alpha-synuclein and is useful for the localization and detection ofalpha-synuclein.Syntaxin 1A Rabbit MonoclonalCatalog No. Size FormatM3360 0.1ml ConcentrateM3362 0.5ml ConcentrateM3364 1.0ml ConcentrateM3361 7.0ml Ready-to-UseClone: SP36Immunogen: Synthetic peptide corresponding toN-terminus of human syntaxin 1A protein.Application: IHC-PSpecies Reactivity: Human*, Mouse*Isotype: Rabbit IgGControl Tissue: BrainCellular Localization: MembraneSynuclein-beta Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2770 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearE2772 0.5ml Concentrate C-terminus of human synuclein-beta.E2774 1.0ml Concentrate Application: IHC-PE2771 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain (Posterior Cingulated Gyrus)Cellular Localization: Cytoplasm, Presynaptic TerminalsHuman Brain stained withanti-Syntaxin 1A antibody M336Human Brain stained withanti-Synuclein-beta antibody E277Product Description: Syntaxin 1A (STX1A) is potentially involved in docking of synapticvesicles at presynaptic active zones and may play a critical role in neurotransmitterexocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular andmusculoskeletal abnormalities observed in Williams-Beuren syndrome (WBS), a raredevelopmental disorder. It is a contiguous gene deletion syndrome involving genesfrom chromosome band 7q11.23.Product Description: Synucleins are small, soluble proteins expressed primarily in neuraltissue and in certain tumors. The family includes three known proteins: alpha-synuclein,beta-synuclein, and gamma-synuclein. The alpha- and beta-synuclein proteins arefound primarily in brain tissue, where they are seen mainly in presynaptic terminals. Thisantibody is specific for beta-synuclein and does not cross react with alpha-and gammasynuclein.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 99

Synuclein-pan Rabbit PolyclonalCatalog No. Size Format Clone: N/AE2790 0.1ml Concentrate Immunogen: Synthetic peptide derived from nearE2792 0.5ml Concentrate N-terminus of human α, β, γ-Synuclein protein.E2794 1.0ml Concentrate Application: IHC-PE2791 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Brain (Posterior Cingulated Gyrus)Cellular Localization: Cytoplasm, Presynaptic TerminalsTdT Rabbit PolyclonalCatalog No. Size FormatE19230 0.1ml ConcentrateE19232 0.5ml ConcentrateE19234 1.0ml ConcentrateE19231 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide in the internal region ofhuman TdT protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Thymus, ThymomasCellular Localization: NucleusHuman Brain stained withanti-Synuclein-pan antibody E279Human Thymus stained withanti-TdT antibody E1923Product Description: Synucleins are small, soluble proteins expressed primarily in neuraltissue and in certain tumors. The family includes three known proteins: alpha-synuclein,beta-synuclein, and gamma-synuclein. The alpha- and beta- synuclein proteins arefound primarily in brain tissue. This antibody detects all synuclein proteins.Product Description: Terminal deoxynucleotidyl transferase (TdT) is a nuclear DNApolymerase responsible for the template-independent addition of N-nucleotides. Itis expressed in normal hematopoietic cells, normal cortical thymocytes (immature,pre-B, pre-T lymphoid cells), and megakaryocytes of the bone marrow as well as acutelymphoblastic leukemia/lymphoma cells.Tau (Neuronal Marker) Rabbit MonoclonalCatalog No. Size FormatM3700 0.1ml ConcentrateM3702 0.5ml ConcentrateM3704 1.0ml ConcentrateM3701 7.0ml Ready-to-UseClone: SP70Immunogen: Synthetic peptide corresponding toN-terminus of human tau protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: CytoplasmThioredoxin Reductase 2 (TrxR2) Rabbit PolyclonalCatalog No. Size FormatE3610 0.1ml ConcentrateE3612 0.5ml ConcentrateE3614 1.0ml ConcentrateE3611 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human TrxR2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Tau (Neuronal Marker) antibody M370Human Liver Carcinoma stained withanti-Thioredoxin Reductase 2 (TrxR2) antibody E361Product Description: Tau is a neuronal microtubule associated protein found mainlyon axons. Its function is to promote tubulin polymerization and stabilize microtubulesand to link certain signaling pathways to the cytoskeleton. Tau is the major componentof paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer’s disease (AD)brain. Hyperphosphorylation impairs the microtubule binding function of tau, resultingin the destruction of microtubules and neurons in AD brain.Product Description: The reduced form of thioredoxin serves as a hydrogen donor,contributes to the up-regulation of various transcription factors and binds to andinhibits MAPKKK activity. Oxidized thioredoxin is converted back to the reduced form byThioredoxin Reductase (TrxR2), a redox active enzyme. There are two distinct isoforms inmammalian cells, TrxR1, a cytosolic protein and TrxR2, almost exclusively a mitochondrialprotein. TrxR expression is induced in several tumors.Tau (Neuronal Marker) Rabbit PolyclonalCatalog No. Size FormatE18290 0.1ml ConcentrateE18292 0.5ml ConcentrateE18294 1.0ml ConcentrateE18291 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toN-terminus of human tau protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Alzheimer’s Brain, Breast CarcinomaCellular Localization: CytoplasmHuman Breast Carcinoma stained withanti-Tau (Neuronal Marker) antibody E1829Thrombin R/ATAP2 Rabbit PolyclonalCatalog No. Size FormatE12530 0.1ml ConcentrateE12532 0.5ml ConcentrateE12534 1.0ml ConcentrateE12531 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to near N-terminus of humanthrombin R.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PancreasCellular Localization: Cytoplasm, MembraneControl Lysate: HeLaMolecular Weight: 66 kDaHuman Pancreas stained withanti-Thrombin R/ATAP2 antibody E1253Product Description: Tau is a neuronal microtubule associated protein found mainlyon axons. Its function is to promote tubulin polymerization and stabilize microtubulesand to link certain signaling pathways to the cytoskeleton. Tau is the major componentof paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer’s disease (AD)brain. Hyperphosphorylation impairs the microtubule binding function of tau, resultingin the destruction of microtubules and neurons in AD brain.Product Description: Thrombin is a serine protease that is involved in plateletaggregation and blood coagulation. It is cleaved from its precursor, prothrombin, andconverts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediatesits regulatory effects by activating cell surface G-protein coupled receptors whichinclude PAR-1, PAR-2 and PAR3.100Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesThymidylate Synthase (TS) Rabbit MonoclonalCatalog No. Size FormatM4120 0.1ml ConcentrateM4122 0.5ml ConcentrateM4124 1.0ml ConcentrateM4121 7.0ml Ready-to-UseClone: SP112Immunogen: Synthetic peptide near C-terminus ofhuman TS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon Carcinoma, TonsilCellular Localization: Cytoplasm, NucleusThyroglobulin Mouse MonoclonalClone: SPM517Catalog No. Size FormatE16600 0.1ml Concentrate Immunogen: Human thyroglobulin.E16602 0.5ml Concentrate Application: IHC-PE16604 1.0ml ConcentrateSpecies Reactivity: Human*E16601 7.0ml Ready-to-UseIsotype: Mouse IgG1Control Tissue: ThyroidCellular Localization: CytoplasmHuman Colon Carcinoma stained withanti-Thymidylate Synthase (TS) antibody M412Human Thyroid stained withanti-Thyroglobulin antibody E1660Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate(dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNAbiosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplasticdrugs that are widely used in the treatment of solid tumors. Overexpressionof TS has been demonstrated in many types of tumors including breast, colon, gastricand melanoma.Product Description: Thyroglobulin is expressed in thyroid carcinomas of the papillaryand follicular types. Adenocarcinomas of non-thyroid origin are not reactive.Thymidylate Synthase (TS) Rabbit PolyclonalCatalog No. Size FormatE18570 0.1ml ConcentrateE18572 0.5ml ConcentrateE18574 1.0ml ConcentrateE18571 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human TS protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, NucleusThyroid Hormone Receptor alpha1/alpha2 Rabbit PolyclonalCatalog No. Size FormatE4060 0.1ml ConcentrateE4062 0.5ml ConcentrateE4064 1.0ml ConcentrateE4061 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human TR-alpha 1 protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ThyroidCellular Localization: NucleusHuman Colon Carcinoma stained withanti-Thymidylate Synthase (TS) antibody E1857Human Thyroid stained withanti-Thyroid Hormone Receptor alpha1/alpha2 antibody E406Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate(dUMP) to deoxythymidine monophosphate (dTMP) during DNA biosynthesis. TS is acritical target for the fluoropyrimidines, an important group of antineoplastic drugs inthe treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumorcells to FdUMP which inactivates TS. Over expression of TS has been demonstrated inmany types of tumors including breast, colon, gastric and melanoma.Product Description: Thyroid hormone receptors (TRs) are ligand dependent membersof the steroid/retinoic acid superfamily of transcription factors. The two genes encodingTRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) withhalf site binding motifs in the orientation of palindromes, direct repeats, or invertedpalindromes.Thymidylate Synthase (TS) Rabbit PolyclonalCatalog No. Size FormatE17910 0.1ml ConcentrateE17912 0.5ml ConcentrateE17914 1.0ml ConcentrateE17911 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tohuman TS.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: Cytoplasm, NucleusThyroid Hormone Receptor beta Rabbit PolyclonalCatalog No. Size FormatE4070 0.1ml ConcentrateE4072 0.5ml ConcentrateE4074 1.0ml ConcentrateE4071 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human TR-beta protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: ThyroidCellular Localization: NucleusHuman Colon Carcinoma stained withanti-Thymidylate Synthase (TS) antibody E1791Human Thyroid stained withanti-Thyroid Hormone Receptor beta antibody E407Product Description: Thymidylate Synthase (TS) converts deoxyuridine monophosphate(dUMP) to deoxythymidine monophosphate (dTMP) during DNA biosynthesis. TS is acritical target for the fluoropyrimidines, an important group of antineoplastic drugs inthe treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumorcells to FdUMP which inactivates TS. Over expression of TS has been demonstrated inmany types of tumors including breast, colon, gastric and melanoma.Product Description: Thyroid hormone receptors (TRs) are ligand dependent membersof the steroid/retinoic acid superfamily of transcription factors. The two genes encodingTRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) withhalf site binding motifs in the orientation of palindromes, direct repeats, or invertedpalindromes.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 101

Thyroid Stimulating Hormone (TSH) Rabbit PolyclonalCatalog No. Size Format Clone: N/AE17500 0.1ml Concentrate Immunogen: Purified human TSH.E17502 0.5ml Concentrate Application: IHC-PE17504 1.0ml ConcentrateSpecies Reactivity: Human*E17501 7.0ml Ready-to-UseIsotype: Rabbit IgGControl Tissue: Anterior PituitaryCellular Localization: CytoplasmTIMP-2 Rabbit PolyclonalCatalog No. Size FormatE4180 0.1ml ConcentrateE4182 0.5ml ConcentrateE4184 1.0ml ConcentrateE4181 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus half of human TIMP-2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmHuman Anterior Pituitary stained withanti-Thyroid Stimulating Hormone (TSH) antibody E1750Human Colon Carcinoma stained withanti-TIMP-2 antibody E418Product Description: TSH is a pituitary hormone of 28kDa, which stimulates thyroidgrowth and production of thyroid hormones. This antibody labels thyrotropic cells of thepituitary.Product Description: Tissue inhibitor of metalloproteinases-1 and 2 (TIMP-1 and TIMP-2)have similar properties, specifically in inhibiting enzymes of matrix metalloproteinasefamily, and are thought to be of great importance in the maintenance of connectivetissue integrity. TIMP-1 forms a complex with activated interstitial collagenases,stromelysin, and type IV collagenase. TIMPs inhibit the proteolytic invasiveness of tumorcells and normal placental trophoblast cells.TIE-1 Rabbit PolyclonalTIMP-3 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE17520 0.1ml ConcentrateE17522 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human TIE-1.E3850 0.1ml ConcentrateE3852 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human TIMP-3.E17524 1.0ml ConcentrateApplication: IHC-PE3854 1.0ml ConcentrateApplication: IHC-PE17521 7.0ml Ready-to-UseSpecies Reactivity: Human*E3851 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: TonsilControl Tissue: Breast Carcinoma, PlacentaCellular Localization: MembraneCellular Localization: CytoplasmHuman Tonsil stained withanti-TIE-1 antibody E1752Human Placenta stained withanti-TIMP-3 antibody E385Product Description: TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1)and TIE2/Tek are expressed primarily on endothelial and hematopoietic progenitorcells, and play important roles in angiogenesis, vasculogenesis, and hematopoiesis.In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Twoligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. The in vivofunctions of TIE1 are related to endothelial cell differentiation.Product Description: TIMPs (Tissue Inhibitors of Metallo Proteinases) form tightinhibitory complexes with MMPs which are involved in matrix degradation and hence, intumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumorcells and normal placental trophoblast cells.TIMP-1 Rabbit PolyclonalTNF-R2 Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE3360 0.1ml ConcentrateE3362 0.5ml ConcentrateImmunogen: Synthetic peptide from N-terminus half ofhuman TIMP-1.E4320 0.1ml ConcentrateE4322 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of mouse TNF-R2.E3364 1.0ml ConcentrateApplication: IHC-PE4324 1.0ml ConcentrateApplication: IHC-PE3361 7.0ml Ready-to-UseSpecies Reactivity: Human*E4321 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Bladder, Breast, Colon Carcinoma,Ovarian CarcinomaCellular Localization: CytoplasmControl Tissue: Liver Carcinoma, ThymusCellular Localization: CytoplasmHuman Colon Carcinoma stained withanti-TIMP-1 antibody E336Human Liver Carcinoma stained withanti-TNF-R2 antibody E432Product Description: Tissue inhibitor of metalloproteinases-1 and 2 (TIMP-1 and TIMP-2)have similar properties, specifically in inhibiting enzymes of matrix metalloproteinasefamily, and are thought to be of great importance in the maintenance of connectivetissue integrity. TIMP-1 forms a complex with activated interstitial collagenases,stromelysin, and type IV collagenase. TIMPs inhibit the proteolytic invasiveness of tumorcells and normal placental trophoblast cells.Product Description: Tumor necrosis factor (TNF) is a pleiotropic cytokine whosefunction is mediated through two distinct cell surface receptors. These receptors,designated TNF-R1 and TNF-R2, are 55kDa and 75kDa respectively, and are expressed onmost cell types. Signaling through TNF-R2 is confined to cells of the immune system.102Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesTopo II beta Rabbit PolyclonalCatalog No. Size FormatE4140 0.1ml ConcentrateE4142 0.5ml ConcentrateE4144 1.0ml ConcentrateE4141 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant of human topoisomerase IIbeta protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: NucleusTRADD Rabbit PolyclonalCatalog No. Size FormatE4340 0.1ml ConcentrateE4342 0.5ml ConcentrateE4344 1.0ml ConcentrateE4341 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human TRADD.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Liver CarcinomaCellular Localization: CytoplasmHuman Tonsil stained withanti-Topo II beta antibody E414Human Liver Carcinoma stained withanti-TRADD antibody E434Product Description: Topo II beta plays important roles in synthesis and transcriptionof DNA as well as chromosomal segregation during mitosis. It is present ubiquitously innormal cells and is upregulated in tumors and proliferating cells.Product Description: TRADD (TNF-R1-associated death domain) is an importantmediator of FAS and TNF-r1 associated apoptotic signaling. It contains a deathdomain homologous region (DDH) through which it binds to both FAS and TNF-R1.Overexpression of TRADD causes NFkB activation and apoptosis in absence of TNF.Topoisomerase I Rabbit PolyclonalCatalog No. Size FormatE18860 0.1ml ConcentrateE18862 0.5ml ConcentrateE18864 1.0ml ConcentrateE18861 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human topoisomerase I protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, NucleusTransforming Growth Factor beta R3 Rabbit PolyclonalCatalog No. Size FormatE11230 0.1ml ConcentrateE11232 0.5ml ConcentrateE11234 1.0ml ConcentrateE11231 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant human TGF beta Type IIIreceptor protein encoding aa 644-850 expressed in E.coli.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-Topoisomerase I antibody E1886Human Placenta stained withanti-Transforming Growth Factor beta R3 antibody E1123Product Description: Topoisomerases are nuclear enzymes involved in chromosomecondensation, DNA replication, transcription, recombination and segregation duringmitosis. Human topoisomerase I is a 100kDa protein capable of relaxing positivelyand negatively supercoiled DNA by performing a transient single stranded nick.Autoantibodies for both DNA topoisomerases I and II were found to in the sera ofpatients with certain autoimmune diseases such as systemic lupus erythematosus.Product Description: A total of three members of the TGF beta family, TGF beta1, TGFbeta2 and TGF beta3, have been identified in mammals. The type III TGF beta receptor,also referred to as beta-glycan, confers an additional level of regulation to the TGFbeta signaling system by influencing the affinity with which the TGF betas bind to theirrespective receptors.Toxoplasma Gondii Rabbit PolyclonalCatalog No. Size Format Clone: N/AE4520 0.1ml Concentrate Immunogen: Toxoplasma gondii.E4522 0.5ml Concentrate Application: IHC-PE4524 1.0ml ConcentrateSpecies Reactivity: Human*E4521 7.0ml Ready-to-UseIsotype: Rabbit IgGControl Tissue: Toxoplasma Gondii Infected TissueCellular Localization: NucleusTransforming Growth Factor beta1 (TGFb1) Rabbit PolyclonalCatalog No. Size FormatE11260 0.1ml ConcentrateE11262 0.5ml ConcentrateE11264 1.0ml ConcentrateE11261 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant protein located in aa177-391 of human TGF beta 1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: Cytoplasm, MembraneHuman Toxoplasma Gondii Infected Brain stained withanti-Toxoplasma Gondii antibody E452Human Placenta stained withanti-Transforming Growth Factor beta1 (TGFb1) antibody E1126Product Description: Toxoplasma is as an intracellular parasite in various tissuesof vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Toxoplasma infects tissueof the GI tract where an active infection is accompanied by fever and enlargementof the spleen. Symptoms of toxoplasmosis are generally mild. However congenitaltoxoplasmosis can produce blindness or mental retardation in the newborn.Product Description: A total of three members of the TGF beta family, TGF beta1,TGF beta2 and TGF beta3, have been identified in mammals. TGF betas mediate theiractivity by high affinity binding to the type II receptor transmembrane protein with acytoplasmic serine/threonine kinase domain.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 103

Transforming Growth Factor beta2 (TGFb2) Rabbit PolyclonalCatalog No. Size FormatE18020 0.1ml ConcentrateE18022 0.5ml ConcentrateE18024 1.0ml ConcentrateE18021 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived from internalregion of human TGF beta 2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmTransglutaminase II Rabbit PolyclonalCatalog No. Size FormatE4170 0.1ml ConcentrateE4172 0.5ml ConcentrateE4174 1.0ml ConcentrateE4171 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant full length humantransglutaminase II protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, Extracellular Matrix,MembraneHuman Placenta stained withanti-Transforming Growth Factor beta2 (TGFb2) antibody E1802Human Breast Carcinoma stained withanti-Transglutaminase II antibody E417Product Description: Transforming growth factor betas (TGFbs) mediate many cell-cellinteractions that occur during embryonic development. Three TGFbs (TGFb1, TGFb2 andTGFb3) have been identified in mammals. They are synthesized as precursor proteinsand then cleaved to yield a 112 amino acid polypeptide. This antibody reacts with theprecursor and mature form of TGFb2 and to a lesser extent TGF3, but does not crossreact with TGFb1.Product Description: TGase II is implicated in programmed cell death, signaltransduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion,cataract formation, and wound healing.Transforming Growth Factor beta3 (TGFb3) Rabbit PolyclonalCatalog No. Size FormatE3940 0.1ml ConcentrateE3942 0.5ml ConcentrateE3944 1.0ml ConcentrateE3941 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of the precursor form of human TGF beta 3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: CytoplasmHuman Placenta stained withanti-Transforming Growth Factor beta3 (TGFb3) antibody E394TTF-1 Rabbit PolyclonalCatalog No. Size FormatE19180 0.1ml ConcentrateE19182 0.5ml ConcentrateE19184 1.0ml ConcentrateE19181 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide in theinternal region of human TTF-1 protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung, LungAdenocarcinoma, ThyroidCellular Localization: NucleusControl Lysate: HeLaMolecular Weight: 39 kDaHuman Thyroid stained withanti-TTF-1 antibody E1918Product Description: A total of three members of the TGF beta family, TGF beta1, TGFbeta2 and TGF beta3, have been identified in mammals. The type III TGF beta receptor,also referred to as beta-glycan, confers an additional level of regulation to the TGFbeta signaling system by influencing the affinity with which the TGF betas bind to theirrespective receptors.Product Description: Thyroid transcription factor-1 (TTF-1) is a member of the NKx2family of homeodomain transcription factors. It binds and activates the promoter ofthyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor.TTF-1 is important in the maintenance of thyroid differentiation and lung development.It is expressed in epithelial cells of the thyroid gland and the lung, and tumors derivedfrom such epithelial cells.Transforming Growth Factor R1 Rabbit PolyclonalCatalog No. Size FormatE12340 0.1ml ConcentrateE12342 0.5ml ConcentrateE12344 1.0ml ConcentrateE12341 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding tointernal region of human TGF beta R1.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PlacentaCellular Localization: MembraneTTF-1 Mouse MonoclonalCatalog No. Size FormatE17080 0.1ml ConcentrateE17082 0.5ml ConcentrateE17084 1.0ml ConcentrateE17081 7.0ml Ready-to-UseClone: 8G7G3/1Immunogen: Rat TTF-1 recombinant protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Mouse IgG1/kControl Tissue: Lung, ThyroidCellular Localization: NucleusHuman Placenta stained withanti-Transforming Growth Factor R1 antibody E1234Human Lung stained withanti-TTF-1 antibody E1708Product Description: A total of three members of the TGF beta family, TGF beta1, TGFbeta2 and TGF beta3, have been identified in mammals. Each is synthesized as a latentprecursor that is subsequently cleaved forming the 112 amino acid growth factor whichbecomes active upon dimerization. TGFbetas mediate their activity by high affinitybinding to the type II receptor 70kDa transmembrane protein with a cytoplasmic serine/threonine kinase domain.Product Description: TTF-1 (Thyroid transcription factor-1) is a member of the NKx2family of homeodomain transcription factors. It is expressed in epithelial cells of thethyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon,kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. TTF-1is detected in primary lung adenocarcinomas and small cell carcinomas and is absent incolon and breast carcinomas.104Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesTubulin-alpha Rabbit PolyclonalCatalog No. Size Format Clone: N/AE4130 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE4132 0.5ml Concentrate C-terminus of human tubulin alpha.E4134 1.0ml Concentrate Application: IHC-PE4131 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: LungCellular Localization: CytoplasmUbiquitin Rabbit PolyclonalCatalog No. Size FormatE3350 0.1ml ConcentrateE3352 0.5ml ConcentrateE3354 1.0ml ConcentrateE3351 7.0ml Ready-to-UseClone: N/AImmunogen: Recombinant full length humanubiquitin protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Alzheimer’s Brain, PancreasCellular Localization: Cytoplasm, Membrane, NucleusHuman Lung stained withanti-Tubulin-alpha antibody E413Human Alzheimer’s Brain stained withanti-Ubiquitin antibody E335Product Description: Microtubules, the major cytoskeletal elements found in alleukaryotic cells, consist of tubulin, which is a dimer of two 55kDa subunits: alpha andbeta. Microtubules play key roles in chromosome segregation in mitosis, intracellulartransport, ciliary and flagellar bending, and structural support of the cytoskeleton.Product Description: Ubiquitin (Ub) plays a very important role in regulated nonlysosomalATP dependent protein degradation. The protein to be degraded isconjugated to Ub and the ubiquinated protein is then selectively degraded by a 26Scomplex, multicatalytic cytosolic and nuclear protease termed proteasome. TheUb-proteasome proteolytic pathway, which is a complex process, is implicated to be ofgreat importance for regulating numerous cellular processes.Tubulin-beta Rabbit PolyclonalCatalog No. Size Format Clone: N/AE3810 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE3812 0.5ml Concentrate C-terminus of human tubulin beta.E3814 1.0ml Concentrate Application: IHC-PE3811 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Lung, SkinCellular Localization: CytoplasmUrocortin Rabbit PolyclonalCatalog No. Size FormatE4480 0.1ml ConcentrateE4482 0.5ml ConcentrateE4484 1.0ml ConcentrateE4481 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of urocortin.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: PituitaryCellular Localization: CytoplasmHuman Lung stained withanti-Tubulin-beta antibody E381Human Pituitary stained withanti-Urocortin antibody E448Product Description: Microtubules, the major cytoskeletal elements found in alleukaryotic cells, consist of tubulin, which is a dimer of two 55kDa subunits: alpha andbeta. Microtubules play key roles in chromosome segregation in mitosis, intracellulartransport, ciliary and flagellar bending, and structural support of the cytoskeleton. Thisantibody blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) topromote a stable inhibition of adenylyl cyclase.Product Description: Urocortin (UCN), a neuropeptide widely distributed in the centralnervous system (CNS), belongs to a family of structurally related peptides includingcorticotrophin releasing factor (CRF), sauvagine and urotensin I. Urocortin is derivedfrom a larger precursor protein (pro-urocortin). Urocortin has biological actions similarto CRF. It is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion bycorticotroph cells from the anterior pituitary.Tubulin-gamma Rabbit PolyclonalCatalog No. Size Format Clone: N/AE10330 0.1ml Concentrate Immunogen: Synthetic peptide derived fromE10332 0.5ml Concentrate C-terminus of human tubulin gamma protein.E10334 1.0ml Concentrate Application: IHC-PE10331 7.0ml Ready-to-Use Species Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Colon CarcinomaCellular Localization: CytoplasmUroplakin III Rabbit MonoclonalCatalog No. Size FormatM3730 0.1ml ConcentrateM3732 0.5ml ConcentrateM3734 1.0ml ConcentrateM3731 7.0ml Ready-to-UseClone: SP73Immunogen: Synthetic peptide corresponding toC-terminus of human uroplakin III protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bladder, Bladder CarcinomaCellular Localization: Cytoplasm, MembraneHuman Colon Carcinoma stained withanti-Tubulin-gamma antibody E1033Human Bladder Carcinoma stained withanti-Uroplakin III antibody M373Product Description: Tubulin gamma, an essential structural protein for cell growth,organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma-depletedcells fail to form functional spindles and arrest during nuclear division. Tubulin gammaassociates with spindle body components Spc97 and Spc98 to form the tubulin gammacomplex. The phosphorylation of tubulin gamma at Tyr445 plays a regulatory role inmicrotubule formation.Product Description: Uroplakins are specific differentiation products of terminallydifferentiated superficial urothelial cells. Uroplakin III is expressed in primary andmetastatic urothelial carcinomas.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 105

Uroplakin III Rabbit PolyclonalCatalog No. Size FormatE18240 0.1ml ConcentrateE18242 0.5ml ConcentrateE18244 1.0ml ConcentrateE18241 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to C-terminus of humanuroplakin III.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Bladder, Bladder CarcinomaCellular Localization: Cytoplasm, MembraneControl Lysate: Human BladderMolecular Weight: 32 kDaHuman Bladder Carcinoma stained withanti-Uroplakin III antibody E1824Vascular Endothelial Growth Factor (VEGF) Rabbit PolyclonalCatalog No. Size FormatE2610 0.1ml ConcentrateE2612 0.5ml ConcentrateE2614 1.0ml ConcentrateE2611 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromN-terminus of human VEGF.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AngiosarcomaCellular Localization: Cytoplasm, Extracellular Matrix,MembraneHuman Angiosarcoma stained withanti-Vascular Endothelial Growth Factor (VEGF) antibody E261Product Description: Uroplakins are specific differentiation products of terminallydifferentiated superficial urothelial cells. Uroplakin III is expressed in primary andmetastatic urothelial carcinomas.Product Description: VEGF (Vascular Endothelial Growth Factor) is a homodimeric,disulfide-linked glycoprotein involved in angiogenesis which promotes tumorprogression and metastasis. This antibody reacts with the 165, 189 and 121 amino acidsplice variants of human VEGF.USF-2 Rabbit PolyclonalVillin Rabbit PolyclonalCatalog No. Size FormatClone: N/ACatalog No. Size FormatClone: N/AE12560 0.1ml ConcentrateE12562 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding to aa327-346 of mouse USF-2.E16640 0.1ml ConcentrateE16642 0.5ml ConcentrateImmunogen: Synthetic peptide corresponding toC-terminus of human villin.E12564 1.0ml ConcentrateE12561 7.0ml Ready-to-UseApplication: IHC-PSpecies Reactivity: Human*E16644 1.0ml ConcentrateE16641 7.0ml Ready-to-UseApplication: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Breast CarcinomaControl Tissue: Small IntestineCellular Localization: NucleusCellular Localization: Cytoplasm, MembraneHuman Breast Carcinoma stained withanti-USF-2 antibody E1256Human Small Intestine stained withanti-Villin antibody E1664Product Description: The ubiquitously expressed cellular upstream stimulatory factor(USF) consists of 43kDa (USF-1) and 44kDa (USF-2) polypeptides which independentlyexhibit site-specific DNA binding and are members of the c-Myc-related family ofregulatory factors containing helix-loop-helix domains. USF also contains a leucinerepeat that is required for efficient DNA binding.Product Description: Villin is an actin binding protein expressed mainly in the brushborder of the epithelium in vertebrates. Villin can cap, nucleate, sever and bundle actinin a Ca2+ and phosphoinositide-regulated manner. Villin is composed of six repeatsthat together constitute the core domain. The core domain retains the Ca2+ dependentcapping nucleating and severing activity, whereas the headpiece domain contributestowards actin filament bundling and binding F-actin.Vascular Endothelial Growth Factor (VEGF) Rabbit MonoclonalCatalog No. Size FormatN/A N/A N/AN/A N/A N/AN/A N/A N/AM3281 7.0ml Ready-to-UseClone: SP28Immunogen: Synthetic peptide derived fromN-terminus of human VEGF.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: AngiosarcomaCellular Localization: Cytoplasm, Extracellular Matrix,MembraneVimentin Rabbit MonoclonalCatalog No. Size FormatM3200 0.1ml ConcentrateM3202 0.5ml ConcentrateM3204 1.0ml ConcentrateM3201 7.0ml Ready-to-UseClone: SP20Immunogen: Recombinant humanvimentin protein.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Melanoma, SarcomasCellular Localization: CytoplasmControl Lysate: HeLaMolecular Weight: 53 kDaHuman Angiosarcoma stained withanti-Vascular Endothelial Growth Factor (VEGF) antibody M328Human Melanoma stained withanti-Vimentin antibody M320Product Description: VEGF (Vascular Endothelial Growth Factor) is a homodimeric,disulfide-linked glycoprotein involved in angiogenesis which promotes tumorprogression and metastasis. This antibody reacts with the 165, 189 and 121 amino acidsplice variants of human VEGF.Product Description: Vimentin is the main intermediate filament protein inmesenchymal cells. This protein is believed to be involved with the intracellulartransport of proteins between the nucleus and plasma membrane. Vimentin isexpressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas aregenerally negative. Melanomas, lymphomas and vascular tumors may express vimentin.106Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

Primary AntibodiesVinculin Rabbit MonoclonalWNT-1 Rabbit PolyclonalCatalog No. Size FormatClone: SP117Catalog No. Size FormatClone: N/AM4170 0.1ml ConcentrateM4172 0.5ml ConcentrateImmunogen: Synthetic peptide derived fromC-terminus of human vinculin protein.E3960 0.1ml ConcentrateE3962 0.5ml ConcentrateImmunogen: Synthetic peptide derivedfrom N-terminus of human WNT-1.M4174 1.0ml ConcentrateApplication: IHC-PE3964 1.0ml ConcentrateApplication: IHC-P, WBM4171 7.0ml Ready-to-UseSpecies Reactivity: Human*E3961 7.0ml Ready-to-UseSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Heart, Small IntestineControl Tissue: Breast CarcinomaCellular Localization: Cytoplasm, MembraneCellular Localization: CytoplasmControl Lysate: NIH3T3Molecular Weight: 42 kDaHuman Heart stained withanti-Vinculin antibody M417Human Breast Carcinoma stained withanti-WNT-1 antibody E396Product Description: Vinculin is a muscle-specific cytoskeletal protein associatedwith the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens typejunctions. It functions as one of several interacting proteins involved in anchoringF-actin to the membrane and regulating cell-surface E-cadherin expression. The defectof this protein can cause dilated cardiomyopathyProduct Description: Wnt-1 is a cysteine-rich secreted glycoprotein that associates withcell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1 isessential for normal development of the embryonic nervous system and its expression isnormally limited to the embryonic neural tube and adult spermatids.Vinculin Rabbit PolyclonalCatalog No. Size FormatE18720 0.1ml ConcentrateE18722 0.5ml ConcentrateE18724 1.0ml ConcentrateE18721 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide derived fromC-terminus of human vinculin protein.Application: IHC-PSpecies Reactivity: Human*XMRV Rabbit PolyclonalCatalog No. Size FormatE19070 0.1ml ConcentrateE19072 0.5ml ConcentrateE19074 1.0ml ConcentrateE19071 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide near N-terminus ofXMRV envelope protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGIsotype: Rabbit IgGControl Tissue: Pancreas, Small IntestineControl Tissue: Prostate Carcinoma with XMRV InfectionCellular Localization: CytoplasmCellular Localization: Cytoplasm, MembraneHuman Pancreas stained withanti-Vinculin antibody E1872Human Prostate Carcinoma with XMRV Infection stained withanti-XMRV antibody E1907Product Description: Vinculin is a cytoskeletal protein associated with the cytoplasmicfaces of both cell-cell and cell extracellular matrix adherens type junctions. It functionsas one of several interacting proteins involved in anchoring F-actin to the membrane.Vinculin represents a key element in the transmembrane linkage of the extracellularmatrix to the cytoplasmic microfilament system.Product Description: Xenotropic murine leukemia virus-related virus (XMRV) is a newlyrecognized human retrovirus first identified in prostate cancer tissues from men with adeficiency in the innate immunity gene RNASEL. At present, studies have detected XMRVDNA and protein in prostate cancer at widely different rates and in patients with chronicfatigue syndrome. The XMRV protein is synthesized in the Golgi by host cells.Wilm’s Tumor (WT) Rabbit PolyclonalCatalog No. Size FormatE3990 0.1ml ConcentrateE3992 0.5ml ConcentrateE3994 1.0ml ConcentrateE3991 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from C-terminus ofhuman Wilm’s tumor protein.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Wilm’s TumorCellular Localization: NucleusXRCC2 Rabbit PolyclonalCatalog No. Size FormatE4010 0.1ml ConcentrateE4012 0.5ml ConcentrateE4014 1.0ml ConcentrateE4011 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide from N-terminus ofhuman XRCC2.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TestisCellular Localization: Cytoplasm, NucleusHuman Wilm’s Tumor stained withanti-Wilm’s Tumor (WT) antibody E399Human Testis stained withanti-XRCC2 antibody E401Product Description: Wilm’s tumor (WT), a sporadic and familial childhood kidneytumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, azinc-finger transcription factor that is essential for the development of the metanephrickidney and the urogenital system. The WT1 gene is normally expressed in fetal kidneyand mesothelioma.Product Description: XRCC2 (X-Ray Cross Complementing 2) is essential for repairingthe double strand DNA breaks by homologous recombination between sisterchromatids. It is homologous to Rad51. Five human homologues of RAD 51; XRCC2,XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complexor several smaller complexes.US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com 107

ZAP-70 Rabbit PolyclonalCatalog No. Size FormatE12520 0.1ml ConcentrateE12522 0.5ml ConcentrateE12524 1.0ml ConcentrateE12521 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptidecorresponding to C-terminus of humanZAP-70.Application: IHC-P, WBSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: TonsilCellular Localization: CytoplasmControl Lysate: JurkatMolecular Weight: 70 kDaHuman Tonsil stained withanti-ZAP-70 antibody E1252Product Description: Mutations in ZAP-70 gene results in a form of Severe CombinedImmunodeficiency Syndrome (SCID) in humans. ZAP-70 is also expressed in a subset ofChronic Lymphocytic Leukemia (CLL).ZO-3 Rabbit PolyclonalCatalog No. Size FormatE17450 0.1ml ConcentrateE17452 0.5ml ConcentrateE17454 1.0ml ConcentrateE17451 7.0ml Ready-to-UseClone: N/AImmunogen: Synthetic peptide corresponding toC-terminus of human ZO-3.Application: IHC-PSpecies Reactivity: Human*Isotype: Rabbit IgGControl Tissue: Small IntestineCellular Localization: MembraneHuman Small Intestine stained withanti-ZO-3 antibody E1745Product Description: Tight junctions are complexes of proteins between the plasmamembrane domains of epithelial and endothelial cells. ZO-3 forms complexes with ZO-1and interacts with occludin and claudins. ZO-2 and ZO-3 are ubiquitously expressedwithin epithelial tight junctions, and unlike ZO-1, which is also expressed at celljunctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.108Applications Legend: IHC-P = Immunohistochemistry (Paraffin); WB = Western Blotting*Only tested species are listed. Based on sequence homology it is likely that this antibody will work in additional species. Please reference www.springbio.com for more details.**Product available to direct customers and Spring-appointed distributors only.

DETECTION AND ANCILLARY PRODUCTSSpring Bioscience offers a complete line of detection systemsoptimized for immunohistochemistry (IHC) and are designedto meet the complex needs of any laboratory.Featuring:• Improved signal to noise ratios• Shorter incubation times• Works with mouse and rabbit primary antibodies• Clean and crisp stainingREVEAL Detection Systems – novel biotin-free chemistriesenable our best-in-class detection systems, including:• REVEAL HRP DAB System• REVEAL HRP AEC System• REVEAL AP Red System

REVEAL Best-in-Class Detection SystemsSpring Bioscience’s novel biotin-free detection system, REVEAL, eliminates the polymerbackbone and allows the enzyme to be conjugated directly to the secondary antibodyby long-arm linkers. The compact size of the molecule enables diffusion through tissuepromoting access to targeted binding sites.p53 (SP5) Cat# M3054, Colon Adenocarcinomap53 (SP5) Cat #M3054, Colon CarcinomaREVEAL AP Conjugate Leading Competitor’s AP Conjugate REVEAL HRP Conjugate Leading Competitor’s HRP PolymerSpring REVEAL Biotin-Free Detection SystemsCatalogNumberProduct Name Size FormatSPA-015 Reveal - Biotin-Free Polyvalent AEC 15ml Ready-to-useSPA-060 Reveal - Biotin-Free Polyvalent AEC 60ml Ready-to-useSPA-125 Reveal - Biotin-Free Polyvalent AEC 125ml Ready-to-useSPD-015 Reveal - Biotin-Free Polyvalent DAB 15ml Ready-to-useSPD-060 Reveal - Biotin-Free Polyvalent DAB 60ml Ready-to-useSPD-125 Reveal - Biotin-Free Polyvalent DAB 125ml Ready-to-usePAR-015 Reveal - Biotin-Free Polyvalent AP (Red) 15ml Ready-to-usePAR-060 Reveal - Biotin-Free Polyvalent AP (Red) 60ml Ready-to-usePAR-125 Reveal - Biotin-Free Polyvalent AP (Red) 125ml Ready-to-useSRA-015 Reveal - Rabbit-Specific AEC 15ml Ready-to-useSRA-060 Reveal - Rabbit-Specific AEC 60ml Ready-to-useSRA-125 Reveal - Rabbit-Specific AEC 125ml Ready-to-useSRD-015 Reveal - Rabbit-Specific DAB 15ml Ready-to-useSRD-060 Reveal - Rabbit-Specific DAB 60ml Ready-to-useSRD-125 Reveal - Rabbit-Specific DAB 125ml Ready-to-useRAR-015 Reveal - Rabbit Specific AP (Red) 15ml Ready-to-useRAR-060 Reveal - Rabbit Specific AP (Red) 60ml Ready-to-useRAR-125 Reveal - Rabbit Specific AP (Red) 125ml Ready-to-useMAR-015 Reveal - Mouse Specific AP (Red) 15ml Ready-to-useMAR-060 Reveal - Mouse Specific AP (Red) 60ml Ready-to-useMAR-125 Reveal - Mouse Specific AP (Red) 125ml Ready-to-useSPB-999 Reveal - Polyvalent HRP 1000ml Ready-to-useSpring Bioscience offers acomplete line of detectionsystems optimized forimmunohistochemistry (IHC)and are designed to meetthe complex needs ofany laboratory.110

Detection and Ancillary ProductsBiotin-Streptavidin Detection SystemsCatalogNumberProduct Name Size FormatTPA-015 Polyvalent HRP AEC Detection System 15ml Ready-to-useTPD-015 Polyvalent HRP DAB Detection System 15ml Ready-to-useTRA-015 Anti-rabbit HRP AEC Detection System 15ml Ready-to-useTRD-015 Anti-rabbit HRP DAB Detection System 15ml Ready-to-useTMA-015 Anti-mouse HRP AEC Detection System 15ml Ready-to-useTMD-015 Anti-mouse HRP DAB Detection System 15ml Ready-to-useHRP-060 Polyvalent HRP Kit 60ml Ready-to-useHRP-125 Polyvalent HRP Kit 125ml Ready-to-useHRP-999 Polyvalent HRP Kit 1000ml Ready-to-useUVX-060 Polyvalent HRP Plus Kit 60ml Ready-to-useUVX-125 Polyvalent HRP Plus Kit 125ml Ready-to-useUVP-060 Polyvalent Alk Phos Kit 60ml Ready-to-useUVP-125 Polyvalent Alk Phos Kit 125ml Ready-to-useDetection ComponentsCatalogNumberProduct Name Size FormatDGP-125 Biotinylated Goat Polyvalent IgG's 125ml Ready-to-useDGP-999 Biotinylated Goat Polyvalent IgG's 1000ml Ready-to-useDGR-125 Biotinylated Goat anti-Rabbit IgG's 125ml Ready-to-useDGM-125 Biotinylated Goat anti-Mouse IgG's 125ml Ready-to-useDSA-125 Streptavidin Alkaline Phosphatase 125ml Ready-to-useDSP-125 Streptavidin Horseradish Peroxidase 125ml Ready-to-useDSP-999 Streptavidin Horseradish Peroxidase 1000ml Ready-to-useDiluent/Buffers/MiscellaneousCatalogNumberProduct Name Size FormatADS-125 Antibody Diluent 125ml Ready-to-usePBS-125 PBS (25x) pH 7.6 125ml ConcentratePBS-999 PBS (25x) pH 7.6 1000ml ConcentratePBT-125 PBS with Tween 20 (20x) 125ml ConcentratePBT-999 PBS with Tween 20 (20x) 1000ml ConcentrateTBS-125 TBS (25x), pH7.4 125ml ConcentrateTBS-999 TBS (25x), pH7.4 1000ml ConcentrateTBT-125 TBS with Tween 20 (20x) 125ml ConcentrateTBT-999 TBS with Tween 20 (20x) 1000ml ConcentrateDMM-060 Mounting Media 60ml Ready-to-useDMM-125 Mounting Media 125ml Ready-to-useUS Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com111

Epitope Recovery/Pretreatment ProductsCatalogNumberProduct Name Size FormatPMB1-125 Antigen Retrieval Buffer 1 (100x) pH=6.0 125ml ConcentratePMB1-250 Antigen Retrieval Buffer 1 (100x) pH=6.0 250ml ConcentratePMB2-125 Antigen Retrieval Buffer 2 (100x) pH=8.0 125ml ConcentratePMB2-250 Antigen Retrieval Buffer 2 (100x) pH=8.0 250ml ConcentratePMB3-125 Antigen Retrieval Buffer 3 (100x) pH=10.0 125ml ConcentratePMB3-250 Antigen Retrieval Buffer 3 (100x) pH=10.0 250ml ConcentratePMB4-125 Antigen Retrieval Buffer 4 (100x) pH=9.0 125ml ConcentratePMB4-250 Antigen Retrieval Buffer 4 (100x) pH=9.0 250ml ConcentrateDCB-125 Citrate Buffer (10x) pH 6.0 125ml ConcentrateDCBX-050 Citrate Buffer (100x) pH 6.0 50ml ConcentrateDEB-125 EDTA Buffer (10x) pH 8.0 125ml ConcentrateDEBX-050 EDTA Buffer (100x) pH 8.0 50ml ConcentrateDTB-125 Tris Buffer (10x) pH 10.0 125ml ConcentrateDTBX-050 Tris Buffer (100x) pH 10.0 50ml ConcentrateDTS-007 Trypsin (2 components) 7ml ConcentrateDPS-007 Pepsin 7ml Ready-to-usePRK-004 Proteinase K 4ml Ready-to-useBlocking ReagentsCatalogNumberProduct Name Size FormatDHP-060 Hydrogen Peroxide Block 60ml Ready-to-useDHP-125 Hydrogen Peroxide Block 125ml Ready-to-useDPB-060 Protein Block 60ml Ready-to-useDPB-125 Protein Block 125ml Ready-to-useBRB-050 Background Reducing Buffer 50ml Ready-to-useDBB-015 Avidin/Biotin Blocking System (2 components) 15ml Ready-to-useChromogensCatalogNumberProduct Name Size FormatASS-060 AEC Single Solution 60ml Ready-to-useASS-125 AEC Single Solution 125ml Ready-to-useDAB-060 DAB (2 components, 50x) 60ml ConcentrateDAB-125 DAB (2 components, 50x) 125ml ConcentrateDAB-999 DAB (2 components, 50x) 1000ml ConcentrateDABD-999 DAB (2 components, 25X) 1000ml ConcentrateLFR-060 Liquid Fast Red Substrate System (75x) 60ml ConcentrateLFR-125 Liquid Fast Red Substrate System (75x) 125ml ConcentrateLFR-999 Liquid Fast Red Substrate System (75x) 1000ml ConcentrateLFRD-999 Liquid Fast Red Substrate System (25x) 1000ml Concentrate112

ANTIBODY MICROARRAY PRODUCTSAntibody microarrays provide researchers the convenience ofdetecting and analyzing multiple proteins simultaneously on asingle slide.Formats Include:• Master Array (650+ antibodies)• Arrays by Pathway (Angiogenesis, Apoptosis,Cancer Marker, Cell Cycle, Cytokine Array,Hematopoiesis, Hormone, Signal Transduction,and Stem Cell)Research Applications Include:• Protein expression profiling• Comparison profiles of normal, diseased, or treated samples• Identification of candidate biomarkersResearcher Benefits Include:• Time savings• Reduction in reagent costs• Reduced variability• Flexible sample preparations (cell extracts,tissue lysates, treated samples)• Built-in control

Array Options to Meet Your Varied Research InterestsAntibody microarrays provide a high-throughput platform for sensitive, efficient and accurateprotein expression profiling, screening and comparison between normal and diseased ortreated samples. Proteins from cell extracts, tissue lysates or treated samples may be used foranalysis and detection. Antibody microarrays enable the qualitative measurement of proteinexpression.Spring’s Master Antibody Microarray kit consists of a set of two slides containing over 650antibodies printed in duplicate. These high affinity antibodies are well characterized forimmunohistochemistry or Western blot.Spring’s antibody microarrays by pathway are designed for researchers interested in a specificfield such as cancer markers. Each pathway microarray kit contains two identical subarrays(one subarray for normal sample and another for the treated sample). Each subarray contains6 replicates of each antibody. Positive and negative controls are included on each subarray.New Master Array coming in 2012!Microarray ProductsCatalogNumberProduct NameSizeAMD-001 Antibody Microarray Detection Kit 1 kitAMS-700 Master Antibody Microarray Slides (656 Abs) 2 slidesAAG-100 Angiogenesis Microarray Slides (127 Abs) 2 slidesAAP-100 Apoptosis Microarray Slides (71 Abs) 2 slidesACC-100 Cell Cycle Microarray Slides (60 Abs) 2 slidesACK-100 Cytokine Microarray Slides (77 Abs) 2 slidesACM-100 Cancer Marker Microarray Slides (86 Abs) 2 slidesAHM-100 Hormone Microarray Slides (30 Abs) 2 slidesAHP-100 Hematopoiesis Microarray Slides (50 Abs) 2 slidesASC-100 Stem Cell Microarray Slides (58 Abs) 2 slidesAST-100 Signal Transduction Microarray Slides (165 Abs) 2 slidesAntibody microarraysprovide researchers theconvenience of detectingand analyzing multipleproteins simultaneously ona single slide.114

RECOMBINANT PROTEIN PRODUCTSRecombinant proteins – expressed in E. coli and are:• Full length or partial sequence of specific proteins• Fused at N-terminus with GST/His-tag• Analyzed by SDS-PAGE using coomassie Blue staining• Available in 0.1ml (0.1mg/ml) size

CatalogNumberProduct NameDomainP1094 ALK/p80 aa 426-528P7014 BCL-6 aa 1-150P3464 Cadherin E aa 600-707P1174 Calretinin full lengthP2824 Cathepsin D full lengthP3044 CD2 aa 25-209P7044 CD4 aa 26-226P7979 CD4 aa 207-316P3024 CD23 aa 48-248P7122 CD34 full lengthP7054 CD68 full lengthP3394 CD99 full lengthP7982 CD138 aa 18-218P2444 c-erbB2 aa 537-636P1524 Chromogranin A aa 260-454P7978 c-Met aa 18-217P2434 D4GDI full lengthP1644 ER alpha aa 1-300P7977 ERCC1 full lengthP3644 FHIT full lengthP7127 gp100 aa 1-150P7113 GST full lengthP7513 IGF-1R aa 1213-1367P7861 Involucrin aa 420-578P7126 IRF4/MUM1 aa 144-451P3264 Keratin 8 full lengthP7514 LYVE-1 aa 243-323P7120 MAGE-1 full lengthP7522 NY-ESO-1 full lengthP7074 p53 mutant (aa135) full lengthP2014 p53 wild type full lengthP2854 PCNA full lengthP3344 PGP9.5 full lengthP7125 PPAR gamma aa 1-477P2074 Progesterone Receptor (PR) aa 412-562P7935 PSMA aa 1-150P7094 PTEN full lengthP2144 S100 beta full lengthP7121 S100A4 full lengthP7914 SOX9 aa 1-150P7874 TGF beta1 aa 177-391P3354 Ubiquitin full lengthP2944 Vimentin full length116

Product IndexRbMab – SP ClonesActin-beta Rabbit Monoclonal 12ALK/p80 Rabbit Monoclonal 14Androgen Receptor (AR) Rabbit Monoclonal 15BAX Rabbit Monoclonal 16BCL-2 alpha Rabbit Monoclonal 16BOB-1 Rabbit Monoclonal 18C4d Rabbit Monoclonal 19Cadherin-E/E-Cadherin Rabbit Monoclonal 19Cadherin-N/N-Cadherin Rabbit Monoclonal 20Calcitonin Rabbit Monoclonal 20Calpain Rabbit Monoclonal 21Calpastatin Rabbit Monoclonal 21Calretinin Rabbit Monoclonal 22Carbonic Anhydrase 9 (CA-9) Rabbit Monoclonal 22Caveolin-1 Rabbit Monoclonal 24CD3 Rabbit Monoclonal 25CD4 Rabbit Monoclonal 26CD5 Rabbit Monoclonal 26CD7 Rabbit Monoclonal 26CD8 Rabbit Monoclonal 27CD10 Rabbit Monoclonal 27CD19 Rabbit Monoclonal 27CD20 Rabbit Monoclonal 28CD22 Rabbit Monoclonal 28CD23 Rabbit Monoclonal 28CD31 Rabbit Monoclonal 29CD43 Rabbit Monoclonal 30CD44 Std Rabbit Monoclonal 30CD79a Rabbit Monoclonal 32CD99 Rabbit Monoclonal 33CD117/c-kit Rabbit Monoclonal 33CD163 Rabbit Monoclonal 34CD278/ICOS Rabbit Monoclonal 35CDK2 Rabbit Monoclonal 35CDX2 Rabbit Monoclonal 36c-erbB-2/HER-2 Rabbit Monoclonal 37c-erbB-3/HER-3 Rabbit Monoclonal 37Chromogranin A Rabbit Monoclonal 38c-Met Rabbit Monoclonal 40c-Met (Paired 1003) Rabbit Monoclonal 40c-Met (Phospho Y1003) Rabbit Monoclonal 40COX-2 Rabbit Monoclonal 42c-TEN Rabbit Monoclonal 42Cyclin D1 Rabbit Monoclonal 43DOG-1 Rabbit Monoclonal 45Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47ERCC1 Rabbit Monoclonal 48Estrogen Receptor (ER) Rabbit Monoclonal 49F4/80 Rabbit Monoclonal 49FLK-1 (mouse) Rabbit Monoclonal 51FOXA1 Rabbit Monoclonal 52FOXP3 Rabbit Monoclonal 53Glial Fibrillary Acidic Protein (GFAP) Rabbit Monoclonal 54Glypican-3 Rabbit Monoclonal 56hENT1 Rabbit Monoclonal 59IKAROS Rabbit Monoclonal 60J-chain Rabbit Monoclonal 62Keratin 5 Rabbit Monoclonal 62Keratin 6 Rabbit Monoclonal 63Keratin 7 Rabbit Monoclonal 63Keratin 8 Rabbit Monoclonal 63Keratin 10 Rabbit Monoclonal 64Keratin 14 Rabbit Monoclonal 64Keratin 17 Rabbit Monoclonal 64Keratin 18 Rabbit Monoclonal 65Keratin 20 Rabbit Monoclonal 66Ki-67 Rabbit Monoclonal 66LMO2 Rabbit Monoclonal 68MCM2 Rabbit Monoclonal 69Mesothelin Rabbit Monoclonal 71MSH2 Rabbit Monoclonal 73MSH6 Rabbit Monoclonal 74MUM1/IRF4 Rabbit Monoclonal 76Myeloperoxidase (MPO) Rabbit Monoclonal 76Nestin Rabbit Monoclonal 77Nitric Oxide Synthase-Inducible (iNOS) Rabbit Monoclonal 78p120 (catenin delta 1) Rabbit Monoclonal 80p53 Rabbit Monoclonal 81p57 Rabbit Monoclonal 81PAX-5 Rabbit Monoclonal 83PI3K p85 Rabbit Monoclonal 85PLAP Rabbit Monoclonal 86Progesterone Receptor (PR) Rabbit Monoclonal 88Progesterone Receptor (PR) Rabbit Monoclonal 88PSMA Rabbit Monoclonal 90S6 Ribosomal (Phospho S235/236) Rabbit Monoclonal 93S6 Ribosomal (Phospho S240/244) Rabbit Monoclonal 93SLIT2 Rabbit Monoclonal 94SOX-2 Rabbit Monoclonal 96Stathmin Rabbit Monoclonal 98Survivin Rabbit Monoclonal 98Synaptophysin Rabbit Monoclonal 99Syntaxin 1A Rabbit Monoclonal 99Tau (Neuronal Marker) Rabbit Monoclonal 100Thymidylate Synthase (TS) Rabbit Monoclonal 101Uroplakin III Rabbit Monoclonal 105Vascular Endothelial Growth Factor (VEGF) Rabbit Monoclonal 106Vimentin Rabbit Monoclonal 106Vinculin Rabbit Monoclonal 10714-3-3 beta Rabbit Polyclonal 1214-3-3 zeta Rabbit Polyclonal 12AActin-beta Rabbit Monoclonal 12Actin-beta Rabbit Polyclonal 12Actin-Skeletal Muscle Rabbit Polyclonal 12Actin-Skeletal Muscle Mouse Monoclonal 12Actin-Smooth Muscle Rabbit Polyclonal 13Actin-Smooth Muscle Mouse Monoclonal 13Adenomatous Polyposis Coli (APC) Rabbit Polyclonal 13Adrenocorticotrophic Hormone (ACTH) Rabbit Polyclonal 13Adrenocorticotrophic Hormone (ACTH) Mouse Monoclonal 13AKT-1/AKT-2 Rabbit Polyclonal 13AKT-1/PKB (Phospho S473) Rabbit Polyclonal 14ALK/p80 Rabbit Monoclonal 14ALK/p80 Rabbit Polyclonal 14alpha Fetoprotein (AFP) Rabbit Polyclonal 14alpha-1-Antichymotrypsin Rabbit Polyclonal 14alpha-1-Antitrypsin Rabbit Polyclonal 14Amylin Peptide Rabbit Polyclonal 15Amyloid Precursor Protein (APP) Rabbit Polyclonal 15Amyloid-beta/Amyloid A4 Rabbit Polyclonal 15Androgen Receptor (AR) Rabbit Monoclonal 15Androgen Receptor (AR) Rabbit Polyclonal 15Annexin I Rabbit Polyclonal 15Annexin VI Rabbit Polyclonal 16Apoptosis Protease Activating Factor 1 (APAF-1) Rabbit Polyclonal 16BBAK Rabbit Polyclonal 16BAX Rabbit Monoclonal 16BAX Rabbit Polyclonal 16BCL-2 alpha Rabbit Monoclonal 16BCL-2 alpha Rabbit Polyclonal 17BCL-6 Rabbit Polyclonal 17BCL-10 Rabbit Polyclonal 17BCL-10 Rabbit Polyclonal 17BCL-10 Mouse Monoclonal 17BCL-X Rabbit Polyclonal 17BCL-XL Rabbit Polyclonal 18BCL-XL Mouse Monoclonal 18BIM/BOD Rabbit Polyclonal 18Bnip3L Rabbit Polyclonal 18BOB-1 Rabbit Monoclonal 18BOB-1 Rabbit Polyclonal 18Bone Morphogenetic Protein 4 (BMP-4) Rabbit Polyclonal 19CC4d Rabbit Monoclonal 19C4d Rabbit Polyclonal 19c-Abl Rabbit Polyclonal 19Cadherin-E/E-Cadherin Rabbit Monoclonal 19Cadherin-E/E-Cadherin Mouse Monoclonal 19Cadherin-E/E-Cadherin Rabbit Polyclonal 20Cadherin-N/N-Cadherin Rabbit Monoclonal 20Cadherin-N/N-Cadherin Rabbit Polyclonal 20Cadherin-pan Rabbit Polyclonal 20Calbindin D28 Rabbit Polyclonal 20Calcitonin Rabbit Monoclonal 20Calcitonin Rabbit Polyclonal 21Calcium Sensing Receptor (CaSR) Rabbit Polyclonal 21Calpain Rabbit Monoclonal 21Calpain Rabbit Polyclonal 21Calpastatin Rabbit Monoclonal 21Calpastatin Rabbit Polyclonal 21Calretinin Rabbit Monoclonal 22Calretinin Rabbit Polyclonal 22CaM Kinase IV Rabbit Polyclonal 22CaM Kinase Kinase alpha Rabbit Polyclonal 22Carbonic Anhydrase 9 (CA-9) Rabbit Monoclonal 22Carbonic Anhydrase 9 (CA-9) Rabbit Polyclonal 22CAS Rabbit Polyclonal 23Caspase-1 Rabbit Polyclonal 23Caspase-8 Rabbit Polyclonal 23Caspase-10 Rabbit Polyclonal 23Catenin-beta Rabbit Polyclonal 23US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com117

Catenin-gamma Rabbit Polyclonal 23Cathepsin D Rabbit Polyclonal 24Caveolin-1 Rabbit Monoclonal 24Caveolin-1 Rabbit Polyclonal 24Caveolin-1 Rabbit Polyclonal 24CBP/p300-Interacting Transactivator 1 (CITED-1) Rabbit Polyclonal 24CCR7 Rabbit Polyclonal 24CCR8 Rabbit Polyclonal 25CD1 alpha Rabbit Polyclonal 25CD2 Rabbit Polyclonal 25CD3 Rabbit Monoclonal 25CD3 Rabbit Polyclonal 25CD3 zeta Rabbit Polyclonal 25CD4 Rabbit Monoclonal 26CD4 Rabbit Polyclonal 26CD5 Rabbit Monoclonal 26CD5 Rabbit Polyclonal 26CD7 Rabbit Monoclonal 26CD7 Rabbit Polyclonal 26CD8 Rabbit Monoclonal 27CD8 Rabbit Polyclonal 27CD9 Rabbit Polyclonal 27CD10 Rabbit Monoclonal 27CD10 Rabbit Polyclonal 27CD19 Rabbit Monoclonal 27CD19 Rabbit Polyclonal 28CD20 Rabbit Monoclonal 28CD20 Rabbit Polyclonal 28CD22 Rabbit Monoclonal 28CD22 Rabbit Polyclonal 28CD23 Rabbit Monoclonal 28CD23 Rabbit Polyclonal 29CD30 Rabbit Polyclonal 29CD31 Rabbit Monoclonal 29CD31 Rabbit Polyclonal 29CD34 (Endothelial Cell Marker) Rabbit Polyclonal 29CD34 (Endothelial Cell Marker) Mouse Monoclonal 29CD38 Rabbit Polyclonal 30CD40 Rabbit Polyclonal 30CD43 Rabbit Monoclonal 30CD43 Rabbit Polyclonal 30CD44 Std Rabbit Monoclonal 30CD44 Std Rabbit Polyclonal 30CD44 Std Mouse Monoclonal 31CD45/T200/LCA Mouse Monoclonal 31CD45RA Mouse Monoclonal 31CD56 Mouse Monoclonal 31CD56 Mouse Monoclonal 31CD63 Mouse Monoclonal 31CD68 Rabbit Polyclonal 32CD68 Rabbit Polyclonal 32CD68 Rabbit Polyclonal 32CD74 Mouse Monoclonal 32CD79a Rabbit Monoclonal 32CD79a Rabbit Polyclonal 32CD95/FAS Rabbit Polyclonal 33CD99 Rabbit Monoclonal 33CD99/MIC2 Rabbit Polyclonal 33CD105/Endoglin/TGF 1/3 Receptor Rabbit Polyclonal 33CD117/c-kit Rabbit Monoclonal 33CD117/c-kit Rabbit Polyclonal 33CD138 Rabbit Polyclonal 34CD146 Rabbit Polyclonal 34CD150 Rabbit Polyclonal 34CD163 Rabbit Monoclonal 34CD163 Rabbit Polyclonal 34CD168 Rabbit Polyclonal 34CD274/B7-H1 Rabbit Polyclonal 35CD278/ICOS Rabbit Monoclonal 35CD278/ICOS Rabbit Polyclonal 35CDC2 p34 Rabbit Polyclonal 35CDH1 Rabbit Polyclonal 35CDK2 Rabbit Monoclonal 35CDK2 Rabbit Polyclonal 36CDX2 Rabbit Monoclonal 36CDX2 Rabbit Polyclonal 36CEA/CD66e Rabbit Polyclonal 36CEA/CD66e Mouse Monoclonal 36c-erbB-2 (Paired 1248) Rabbit Polyclonal 36c-erbB-2 (Phospho Y1248) Rabbit Polyclonal 37c-erbB-2/HER-2 Rabbit Monoclonal 37c-erbB-2/HER-2 Rabbit Polyclonal 37c-erbB-3/HER-3 Rabbit Monoclonal 37c-erbB-3/HER-3 Rabbit Polyclonal 37c-erbB-3/HER-3 Rabbit Polyclonal 37c-erbB-4/HER-4 Rabbit Polyclonal 38c-Fos Rabbit Polyclonal 38Cholecystokinin (CCK-8) Rabbit Polyclonal 38Chromogranin A Rabbit Monoclonal 38Chromogranin A Rabbit Polyclonal 38Claudin-1 Rabbit Polyclonal 38Claudin-3 Rabbit Polyclonal 39Claudin-4 Rabbit Polyclonal 39Claudin-5 Rabbit Polyclonal 39Claudin-7 Rabbit Polyclonal 39Claudin-10 Rabbit Polyclonal 39Claudin-11 Rabbit Polyclonal 39c-Met Rabbit Monoclonal 40c-Met Rabbit Polyclonal 40c-Met (Paired 1003) Rabbit Monoclonal 40c-Met (Paired 1003) Rabbit Polyclonal 40c-Met (Paired 1349) Rabbit Polyclonal 40c-Met (Phospho Y1003) Rabbit Monoclonal 40c-Met (Phospho Y1003) Rabbit Polyclonal 41c-Met (Phospho Y1234/1235) Rabbit Polyclonal 41c-Met (Phospho Y1349) Rabbit Polyclonal 41Connexin-43 Rabbit Polyclonal 41Corticotropin Releasing Factor (CRF) Rabbit Polyclonal 41COX-1 Rabbit Polyclonal 41COX-2 Rabbit Monoclonal 42COX-2 Rabbit Polyclonal 42CREB-Binding Protein (CBP) Rabbit Polyclonal 42Cripto-1 Rabbit Polyclonal 42c-TEN Rabbit Monoclonal 42c-TEN Rabbit Polyclonal 42Cullin-1 (CUL-1) Rabbit Polyclonal 43Cullin-2 (CUL-2) Rabbit Polyclonal 43CXCR4 Rabbit Polyclonal 43Cyclin B1 Rabbit Polyclonal 43Cyclin D1 Rabbit Monoclonal 43Cyclin D1 Rabbit Polyclonal 43Cyclin E Rabbit Polyclonal 44CYR61 Rabbit Polyclonal 44Cytochrome C Rabbit Polyclonal 44DD4-GDI Rabbit Polyclonal 44D5DR Rabbit Polyclonal 44DAPK2 Rabbit Polyclonal 44Desmin Rabbit Polyclonal 45DFF-45 Rabbit Polyclonal 45DHFR Rabbit Polyclonal 45DJ-1 Rabbit Polyclonal 45DOG-1 Rabbit Monoclonal 45DOG-1 Rabbit Polyclonal 45DOK-1 Rabbit Polyclonal 46DOK-2 Rabbit Polyclonal 46DRAK2 Rabbit Polyclonal 46Dysferlin Rabbit Polyclonal 46Dystrophin Rabbit Polyclonal 46Dystrophin Mouse Monoclonal 46EE2F-2 Rabbit Polyclonal 47EGFL7 Rabbit Polyclonal 47Ephrin A4 Rabbit Polyclonal 47Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47Epidermal Growth Factor Receptor (EGFR) Rabbit Monoclonal 47Epidermal Growth Factor Receptor (EGFR) Mouse Monoclonal 47Epidermal Growth Factor Receptor (EGFR) Rabbit Polyclonal 48ERAB Rabbit Polyclonal 48ERCC1 Rabbit Monoclonal 48ERCC1 Rabbit Polyclonal 48ERCC1 Rabbit Polyclonal 48ERK-1 Rabbit Polyclonal 48ERK-2 Rabbit Polyclonal 49ESA/Ep-CAM Mouse Monoclonal 49Estrogen Receptor (ER) Rabbit Monoclonal 49Estrogen Receptor (ER) Rabbit Polyclonal 49EZH2 Rabbit Polyclonal 49FF4/80 Rabbit Monoclonal 49F4/80 Rabbit Polyclonal 50FAS Ligand Rabbit Polyclonal 50FAS-Associated Phosphatase (FAP-1) Rabbit Polyclonal 50FcRH5 Rabbit Polyclonal 50FGFR-1 Rabbit Polyclonal 50FGFR-2 Rabbit Polyclonal 50FGFR-3 Rabbit Polyclonal 51FLI-1 Rabbit Polyclonal 51FLK-1 (mouse) Rabbit Monoclonal 51FLK-1/KDR/VEGFR-2 Rabbit Polyclonal 51FLT-1/VEGFR1 Rabbit Polyclonal 51FLT-4/VEGFR3 Rabbit Polyclonal 51Fn14 Rabbit Polyclonal 52FOXA1 Rabbit Monoclonal 52FOXA1 Rabbit Polyclonal 52FOXO1A Rabbit Polyclonal 52FOXO3A Rabbit Polyclonal 52FOXP1 Rabbit Polyclonal 52FOXP3 Rabbit Monoclonal 53FOXP3 Rabbit Polyclonal 53FOXP4 Rabbit Polyclonal 53FPGS Rabbit Polyclonal 53FRA-2 Rabbit Polyclonal 53Fragile Histidine Triad (FHIT) Rabbit Polyclonal 53118

Product IndexGGADD153 Rabbit Polyclonal 54Gamma Glutamylcysteine Synthetase (GCS) Rabbit Polyclonal 54GANP Rabbit Polyclonal 54Gastrin-1 Rabbit Polyclonal 54GDNF Rabbit Polyclonal 54Glial Fibrillary Acidic Protein (GFAP) Rabbit Monoclonal 54Glial Fibrillary Acidic Protein (GFAP) Rabbit Polyclonal 55Glial Fibrillary Acidic Protein (GFAP) Mouse Monoclonal 55Glucagon Rabbit Polyclonal 55GluR1 Rabbit Polyclonal 55GluR2/3 Rabbit Polyclonal 55GluR6/7 Rabbit Polyclonal 55GLUT-1 Rabbit Polyclonal 56GLUT-1 Mouse Monoclonal 56GLUT-3 Rabbit Polyclonal 56Glutamic Acid Decarboxylase 65 (GAD65) Rabbit Polyclonal 56Glutamic Acid Decarboxylase 67 (GAD67) Rabbit Polyclonal 56Glypican-3 Rabbit Monoclonal 56Glypican-3 Rabbit Polyclonal 57GNB2 Rabbit Polyclonal 57GP200 (Renal Cell Carcinoma Marker) Mouse Monoclonal 57Granzyme B Rabbit Polyclonal 57GRB2-Associated Binder 1 (GAB-1) Rabbit Polyclonal 57GRP75 Rabbit Polyclonal 57GRP78 Rabbit Polyclonal 58GRP94 Rabbit Polyclonal 58GRSF-1 Rabbit Polyclonal 58GSK-3 Rabbit Polyclonal 58Guanylyl Cyclase beta Rabbit Polyclonal 58HH. Pylori Rabbit Polyclonal 58H. Pylori Mouse Monoclonal 59HDAC1 Rabbit Polyclonal 59HDAC2 Rabbit Polyclonal 59Heat Shock Protein 60 (HSP60) Rabbit Polyclonal 59Heat Shock Protein 70 (HSP70) Rabbit Polyclonal 59hENT1 Rabbit Monoclonal 59HMGA2 Rabbit Polyclonal 60Human Growth Hormone (HGH) Rabbit Polyclonal 60IID2 Rabbit Polyclonal 60ID3 Rabbit Polyclonal 60IKAROS Rabbit Monoclonal 60IKAROS Rabbit Polyclonal 60Inhibin-alpha Rabbit Polyclonal 61Integrin beta 5 Rabbit Polyclonal 61Involucrin Rabbit Polyclonal 61IPO-38 (Proliferation Marker) Mouse Monoclonal 61IRS-1 Rabbit Polyclonal 61JJAB1 Rabbit Polyclonal 61J-chain Rabbit Monoclonal 62J-chain Rabbit Polyclonal 62Jena-Muenchen4 (JM4) Rabbit Polyclonal 62KKappa Light Chain (B Cell Marker) Mouse Monoclonal 62Keratin 5 Rabbit Monoclonal 62Keratin 5 Rabbit Polyclonal 62Keratin 6 Rabbit Monoclonal 63Keratin 6 Rabbit Polyclonal 63Keratin 7 Rabbit Monoclonal 63Keratin 7 Rabbit Polyclonal 63Keratin 8 Rabbit Monoclonal 63Keratin 8 Rabbit Polyclonal 63Keratin 8 Rabbit Polyclonal 64Keratin 10 Rabbit Monoclonal 64Keratin 10 Rabbit Polyclonal 64Keratin 14 Rabbit Monoclonal 64Keratin 14 Rabbit Polyclonal 64Keratin 17 Rabbit Monoclonal 64Keratin 17 Rabbit Polyclonal 65Keratin 18 Rabbit Monoclonal 65Keratin 18 Rabbit Polyclonal 65Keratin 18 Mouse Monoclonal 65Keratin 18 Mouse Monoclonal 65Keratin 19 Rabbit Polyclonal 65Keratin 20 Rabbit Monoclonal 66Keratin 20 Rabbit Polyclonal 66Ki-67 Rabbit Monoclonal 66Ki-67 Rabbit Polyclonal 66KOC/L523S Rabbit Polyclonal 66k-Ras Rabbit Polyclonal 66Ku80 Rabbit Polyclonal 67Ku80 Rabbit Polyclonal 67LLambda Light Chain Rabbit Polyclonal 67Lamin B Rabbit Polyclonal 67Lewis A Mouse Monoclonal 67LIMD1 Rabbit Polyclonal 67Lipocalin-2 Rabbit Polyclonal 68LMO2 Rabbit Monoclonal 68LMO2 Rabbit Polyclonal 68LMO2 Mouse Monoclonal 68Luteinizing Hormone (LH) Rabbit Polyclonal 68MMAGE-1 Rabbit Polyclonal 68MALT-1 Rabbit Polyclonal 69Mammaglobin Rabbit Polyclonal 69MART-1/Melan-A Rabbit Polyclonal 69MCL-1 Rabbit Polyclonal 69MCM2 Rabbit Monoclonal 69MCM2 Rabbit Polyclonal 69MCM2 Rabbit Polyclonal 70MCM5 Rabbit Polyclonal 70MDGI Rabbit Polyclonal 70MDGI Rabbit Polyclonal 70MDM2 Rabbit Polyclonal 70MEF-2 Rabbit Polyclonal 70Melanoma (gp100) Rabbit Polyclonal 71Mesothelin Rabbit Monoclonal 71Mesothelin Rabbit Polyclonal 71mGluR1 Rabbit Polyclonal 71mGluR5 Rabbit Polyclonal 71Microphthalmia (MiTF) Rabbit Polyclonal 71MIP-1 alpha Rabbit Polyclonal 72MLH1 Rabbit Polyclonal 72MMP-1 Rabbit Polyclonal 72MMP-2 (72kDa Collagenase IV) Rabbit Polyclonal 72MMP-3 (Stromelysin-1) Rabbit Polyclonal 72MMP-9 (92kDa Collagenase IV) Rabbit Polyclonal 72MMP-10 (Stromelysin-2) Rabbit Polyclonal 73MMP-14/MT1-MMP Rabbit Polyclonal 73MMP-15/MT2-MMP Rabbit Polyclonal 73MMP-19 Rabbit Polyclonal 73MMP-23 Rabbit Polyclonal 73MSH2 Rabbit Monoclonal 73MSH2 Rabbit Polyclonal 74MSH3 Rabbit Polyclonal 74MSH6 Rabbit Monoclonal 74MSH6 Mouse Monoclonal 74MSH6 Rabbit Polyclonal 74mTOR Rabbit Polyclonal 74mTOR Rabbit Polyclonal 75MUC-1 Underglycosylated Mouse Monoclonal 75MUC-1/EMA/Epithelial Membrane Antigen Rabbit Polyclonal 75MUC-1/EMA/Epithelial Membrane Antigen Mouse Monoclonal 75Mucin 5AC Mouse Monoclonal 75Mucin 5AC Mouse Monoclonal 75Mucin-2/MUC-2 Mouse Monoclonal 76MUM1/IRF4 Rabbit Monoclonal 76MUM1/IRF4 Rabbit Polyclonal 76Myeloperoxidase (MPO) Rabbit Monoclonal 76Myeloperoxidase (MPO) Rabbit Polyclonal 76Myoglobin Rabbit Polyclonal 76NNCK2 Rabbit Polyclonal 77Nephrin Rabbit Polyclonal 77Nestin Rabbit Monoclonal 77Nestin Rabbit Polyclonal 77Nestin Rabbit Polyclonal 77Neuron Specific Enolase (NSE) Rabbit Polyclonal 77NFkB p50 Rabbit Polyclonal 78NFkB p65 Rabbit Polyclonal 78Nitric Oxide Synthase-Endothelial (eNOS) Rabbit Polyclonal 78Nitric Oxide Synthase-Inducible (iNOS) Rabbit Monoclonal 78Nitric Oxide Synthase-Inducible (iNOS) Rabbit Polyclonal 78Nitric Oxide Synthase-Universal (uNOS) Rabbit Polyclonal 78NKX3.1 Rabbit Polyclonal 79Notch-1 Rabbit Polyclonal 79NRF2 Rabbit Polyclonal 79OOccludin Rabbit Polyclonal 79OCT-1 Rabbit Polyclonal 79OCT-2 Rabbit Polyclonal 79OCT-3/OCT-4 Rabbit Polyclonal 80OCT-3/OCT-4 Rabbit Polyclonal 80Osteopontin Rabbit Polyclonal 80Pp120 (catenin delta 1) Rabbit Monoclonal 80p120 (catenin delta 1) Rabbit Polyclonal 80p14ARF Rabbit Polyclonal 80p27Kip1 Rabbit Polyclonal 81p300/CBP Rabbit Polyclonal 81p53 Rabbit Monoclonal 81p53 Rabbit Polyclonal 81p53 Mouse Monoclonal 81p57 Rabbit Monoclonal 81p57 Rabbit Polyclonal 82US Orders Online: www.springbio.com Email: orders@springbio.com Tel: (925) 474-8440 Fax: (925) 474-8469 International Orders Email: intlorders@springbio.com119

p70S6k Rabbit Polyclonal 82p70S6k (Paired 421/424) Rabbit Polyclonal 82p70S6k (Phospho T421/S424) Rabbit Polyclonal 82PAK alpha Rabbit Polyclonal 82Parathyroid Hormone Rabbit Polyclonal 82Parathyroid Hormone Receptor 1 Rabbit Polyclonal 83Parkin Rabbit Polyclonal 83PAX-5 Rabbit Monoclonal 83PAX-5 Rabbit Polyclonal 83Paxillin Rabbit Polyclonal 83PCNA Rabbit Polyclonal 83PD-1/CD279 Rabbit Polyclonal 84PD-1/CD279 Rabbit Polyclonal 84PDCD6 Rabbit Polyclonal 84PDGFR alpha Rabbit Polyclonal 84PDGFR beta Rabbit Polyclonal 84PDK-1 Rabbit Polyclonal 84PDS-1/Securin Rabbit Polyclonal 85Peripheral Myelin Protein (PMP-22) Rabbit Polyclonal 85Phospho-Ser/Thr/Tyr Mouse Monoclonal 85Phosphotyrosine Mouse Monoclonal 85PI3K p110beta Rabbit Polyclonal 85PI3K p85 Rabbit Monoclonal 85PI3K p85 Rabbit Polyclonal 86PIM-1 Rabbit Polyclonal 86PIM-2 Rabbit Polyclonal 86Placental Lactogen (hPL) Rabbit Polyclonal 86PLAP Rabbit Monoclonal 86PLAP Rabbit Polyclonal 86PLC beta 1 Rabbit Polyclonal 87PLC gamma 2 Rabbit Polyclonal 87Pleiotrophin Rabbit Polyclonal 87PML Rabbit Polyclonal 87PMS2 Rabbit Polyclonal 87Podocin Rabbit Polyclonal 87Poly ADP-Ribose Polymerase (PARP) Rabbit Polyclonal 88Potassium Channel Kv3.2 Rabbit Polyclonal 88Presenilin Rabbit Polyclonal 88Progesterone Receptor (PR) Rabbit Monoclonal 88Progesterone Receptor (PR) Rabbit Monoclonal 88Progesterone Receptor (PR) Rabbit Polyclonal 88Prohibitin Rabbit Polyclonal 89Prolactin Rabbit Polyclonal 89Prostate Apoptosis Response 4 (PAR-4) Rabbit Polyclonal 89Prostate Specific Antigen (PSA) Rabbit Polyclonal 89Prostate Stem Cell Antigen (PSCA) Rabbit Polyclonal 89Protein Gene Protein 9.5 (PGP9.5) Rabbit Polyclonal 89PSMA Rabbit Monoclonal 90PSMA Rabbit Polyclonal 90RRACK-1 Rabbit Polyclonal 90RAD51 Rabbit Polyclonal 90RAN Rabbit Polyclonal 90RCAS1 Rabbit Polyclonal 90RCC1 Rabbit Polyclonal 91Retinoblastoma (Rb) Rabbit Polyclonal 91Retinoic Acid Receptor beta Rabbit Polyclonal 91Retinoic X Receptor gamma Rabbit Polyclonal 91RHO Rabbit Polyclonal 91ROC1 Rabbit Polyclonal 91RRM1 Rabbit Polyclonal 92SS100 Rabbit Polyclonal 92S100A4 Rabbit Polyclonal 92S6 Ribosomal Rabbit Polyclonal 92S6 Ribosomal (Paired 235/236) Rabbit Polyclonal 92S6 Ribosomal (Paired 240/244) Rabbit Polyclonal 92S6 Ribosomal (Phospho S235/236) Rabbit Monoclonal 93S6 Ribosomal (Phospho S235/236) Rabbit Polyclonal 93S6 Ribosomal (Phospho S240/244) Rabbit Monoclonal 93S6 Ribosomal (Phospho S240/244) Rabbit Polyclonal 93SHIP-1 Rabbit Polyclonal 93SH-PTP2 Rabbit Polyclonal 93SKP1 p19 Rabbit Polyclonal 94SKP2 p45 Rabbit Polyclonal 94SLIT2 Rabbit Monoclonal 94SLIT2 Rabbit Polyclonal 94SMA Rabbit Polyclonal 94SMAD3 Rabbit Polyclonal 94SMRTe Rabbit Polyclonal 95SOCS-2 Rabbit Polyclonal 95SOCS-3 Rabbit Polyclonal 95SOCS-6 Rabbit Polyclonal 95Sodium Channel-pan Rabbit Polyclonal 95Sonic Hedgehog Rabbit Polyclonal 95SOX-2 Rabbit Monoclonal 96SOX-2 Rabbit Polyclonal 96SOX-4 Rabbit Polyclonal 96SOX-9 Rabbit Polyclonal 96SOX-9 Rabbit Polyclonal 96SOX-11 Rabbit Polyclonal 96SOX-14 Rabbit Polyclonal 97SP1 Rabbit Polyclonal 97ST13 Rabbit Polyclonal 97Stanniocalcin-2 Rabbit Polyclonal 97STAT3 Rabbit Polyclonal 97STAT5 alpha Rabbit Polyclonal 97STAT6 Rabbit Polyclonal 98Stathmin Rabbit Monoclonal 98Stathmin Rabbit Polyclonal 98Survivin Rabbit Monoclonal 98Survivin Rabbit Polyclonal 98SYK Rabbit Polyclonal 98Synaptophysin Rabbit Monoclonal 99Synaptophysin Rabbit Polyclonal 99Syntaxin 1A Rabbit Monoclonal 99Syntaxin 1A Rabbit Polyclonal 99Synuclein-alpha Rabbit Polyclonal 99Synuclein-beta Rabbit Polyclonal 99Synuclein-pan Rabbit Polyclonal 100TTau (Neuronal Marker) Rabbit Monoclonal 100Tau (Neuronal Marker) Rabbit Polyclonal 100TdT Rabbit Polyclonal 100Thioredoxin Reductase 2 (TrxR2) Rabbit Polyclonal 100Thrombin R/ATAP2 Rabbit Polyclonal 100Thymidylate Synthase (TS) Rabbit Monoclonal 101Thymidylate Synthase (TS) Rabbit Polyclonal 101Thymidylate Synthase (TS) Rabbit Polyclonal 101Thyroglobulin Mouse Monoclonal 101Thyroid Hormone Receptor alpha1/alpha2 Rabbit Polyclonal 101Thyroid Hormone Receptor beta Rabbit Polyclonal 101Thyroid Stimulating Hormone (TSH) Rabbit Polyclonal 102TIE-1 Rabbit Polyclonal 102TIMP-1 Rabbit Polyclonal 102TIMP-2 Rabbit Polyclonal 102TIMP-3 Rabbit Polyclonal 102TNF-R2 Rabbit Polyclonal 102Topo II beta Rabbit Polyclonal 103Topoisomerase I Rabbit Polyclonal 103Toxoplasma Gondii Rabbit Polyclonal 103TRADD Rabbit Polyclonal 103Transforming Growth Factor beta R3 Rabbit Polyclonal 103Transforming Growth Factor beta1 (TGFb1) Rabbit Polyclonal 103Transforming Growth Factor beta2 (TGFb2) Rabbit Polyclonal 104Transforming Growth Factor beta3 (TGFb3) Rabbit Polyclonal 104Transforming Growth Factor R1 Rabbit Polyclonal 104Transglutaminase II Rabbit Polyclonal 104TTF-1 Rabbit Polyclonal 104TTF-1 Mouse Monoclonal 104Tubulin-alpha Rabbit Polyclonal 105Tubulin-beta Rabbit Polyclonal 105Tubulin-gamma Rabbit Polyclonal 105UUbiquitin Rabbit Polyclonal 105Urocortin Rabbit Polyclonal 105Uroplakin III Rabbit Monoclonal 105Uroplakin III Rabbit Polyclonal 106USF-2 Rabbit Polyclonal 106VVascular Endothelial Growth Factor (VEGF) Rabbit Monoclonal 106Vascular Endothelial Growth Factor (VEGF) Rabbit Polyclonal 106Villin Rabbit Polyclonal 106Vimentin Rabbit Monoclonal 106Vinculin Rabbit Monoclonal 107Vinculin Rabbit Polyclonal 107WWilm’s Tumor (WT) Rabbit Polyclonal 107WNT-1 Rabbit Polyclonal 107XXMRV Rabbit Polyclonal 107XRCC2 Rabbit Polyclonal 107ZZAP-70 Rabbit Polyclonal 108ZO-3 Rabbit Polyclonal 108120

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