Anisha Patel COSMOS UC Davis Cluster 1: Biotechnology

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2. Obtain a 20bp sequence of the Myb gene that codes forthe transcription factor the myeloblastin protein.3. Find a company such as Thermo Scientific to request thedouble-stranded siRNA to be made.4. Once the sequence is obtained it should be used totransfect the cell culture.Example:Known DNA: ATTCGGCGTAATGC (part of gene thatcodes for myeloblastin)mRNA: UAAGCCGCAUUACGDouble stranded RNA: UAAGCCGCAUUACGAU<strong>UC</strong>GGCGUAAGC
‣ Lipofectamine•Is a common transfection reagent used in cellularbiology to introduce siRNA into in vitro cell cultures bylipofection.-Lipofection: is a technique used to injectgenetic material into a cell by means of liposomes,which are vesicles that can easily merge with the cellmembrane since they are both made of a phospholipidbilayer.5. Use lipofection to transfect the Myeloblastic culturewith the siRNA
Page 6: - During the process of RNAi the do
Page 9 and 10: Initially had difficulty on designi
Page 11 and 12: ‣Myb is the gene thatinfluences l
Page 13: 1. Culture Myeloblastic cells deriv
Page 17 and 18: PART 114. Add 10-15 µl of Laemmli
Page 19 and 20: PART 340. Prepare the chemiluminsce
Page 21 and 22: I would expect the human Myb gene t
Page 23 and 24: -Some studies have suggested that i
Page 25 and 26: Abdelrahim, Maen, Safe, Stephen, Ba
Page 27: http://www.pawelmazur.org/blog/?tag

Anisha Patel COSMOS UC Davis Cluster 1: Biotechnology

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