Anisha Patel COSMOS UC Davis Cluster 1: Biotechnology

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Step 1Myeloblastic Cell CultureStep 2Transfection with siRNAStep 3Extract Proteins from CellStep 4Western Blot

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Step 1Myeloblastic Cell CultureStep 2Transfection with siRNAStep 3Extract Proteins from CellStep 4Western Blot

1. Culture Myeloblastic cells derived from apatient with acute myeloblastic leukemia in aRPMI 1640 medium with 10% fetal bovine serum.10% Fetal bovine serum

Page 6: - During the process of RNAi the do
Page 9 and 10: Initially had difficulty on designi
Page 11: ‣Myb is the gene thatinfluences l
Page 15 and 16: ‣ Lipofectamine•Is a common tra
Page 17 and 18: PART 114. Add 10-15 µl of Laemmli
Page 19 and 20: PART 340. Prepare the chemiluminsce
Page 21 and 22: I would expect the human Myb gene t
Page 23 and 24: -Some studies have suggested that i
Page 25 and 26: Abdelrahim, Maen, Safe, Stephen, Ba
Page 27: http://www.pawelmazur.org/blog/?tag

Anisha Patel COSMOS UC Davis Cluster 1: Biotechnology

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